LOCUS       NZ_CP104673             3257 bp    DNA     circular CON 09-OCT-2023
DEFINITION  Escherichia coli strain 2021CK-00607 plasmid unnamed8, complete
            sequence.
ACCESSION   NZ_CP104673
VERSION     NZ_CP104673.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17815181
            Assembly: GCF_025398375.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Sangster,A., Hergert,J., Hirschi,B., Casey,R., Wagner,J., Young,E.L.
            and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-SEP-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 138.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/09/2023 03:50:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,322
            CDSs (total)                      :: 5,202
            Genes (coding)                    :: 4,979
            CDSs (with protein)               :: 4,979
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 223
            CDSs (without protein)            :: 223
            Pseudo Genes (ambiguous residues) :: 0 of 223
            Pseudo Genes (frameshifted)       :: 81 of 223
            Pseudo Genes (incomplete)         :: 151 of 223
            Pseudo Genes (internal stop)      :: 44 of 223
            Pseudo Genes (multiple problems)  :: 49 of 223
            Pseudo Genes (short protein)      :: 4 of 223
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104673.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2021CK-00607"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed8"
                     /geo_loc_name="USA"
                     /collection_date="2021-01-11"
     gene            complement(72..266)
                     /locus_tag="N5914_RS26490"
                     /old_locus_tag="N5914_26500"
     CDS             complement(72..266)
                     /locus_tag="N5914_RS26490"
                     /old_locus_tag="N5914_26500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041633.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVRFETESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1410..1691)
                     /locus_tag="N5914_RS26495"
                     /old_locus_tag="N5914_26505"
     CDS             complement(1410..1691)
                     /locus_tag="N5914_RS26495"
                     /old_locus_tag="N5914_26505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000586174.1"
                     /translation="MIINIKHKGLRKFAETGNNAGIPQQFTQRLKLILTALSTATNLQD
                     MMDLKALRCHPLKGDRQGEYSLEVNGNWRVVFKLEEPNVFDVDLEDYH"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2036..2332
                     /locus_tag="N5914_RS26500"
                     /old_locus_tag="N5914_26510"
     CDS             2036..2332
                     /locus_tag="N5914_RS26500"
                     /old_locus_tag="N5914_26510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609270.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_000609270.1"
                     /translation="MINLVDPVHPGVFIREMFMEPFEISAADLSEKLHVSPSTLSRVLN
                     GKADLSVEMALRLSRVLGRSAESWINMQTQYSLNASRKSPNVIKELDELVPIF"
                     /name="HigA family addiction module antitoxin"
     gene            complement(2563..2675)
                     /locus_tag="N5914_RS26625"
                     /pseudo=""
     CDS             complement(2563..2675)
                     /locus_tag="N5914_RS26625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     Typing          303..401
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.22e-45"
     Typing          526..781
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.512"
                     /coverage="89.0"
                     /evalue="1.21e-116"
     Typing          3174..3256
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.79e-36"
     Typing          complement(2604..3161)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.286"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acgcgccctg gcgggcttgt ctgctgccga aaacgctcgc ggacaagccg tgagcgcctc
       61 cgtcagcttc gttatgttcc tgtttcactt tcggtctcaa agcgaacctg gatgctgttc
      121 tggagttctt ccgcgagttc gtgcagtcgt tcgcacatgg ccgcctgttc gtcggcatcc
      181 agagcatcca gtttttcaag cagtgtcagg ctctggcttt tgataaatcg cgccatattc
      241 agcgcagttt gttgctgttt gttcaccttc cgttgttctc cgtcagtacc gggtgtcggg
      301 tggaccctga ccaggtggta attgtaagac gttgcgggcg acggtattac aattgcacat
      361 cctgtcctgt ttttttagga cgtatcatgg cacagcatat caactgataa ataatccctg
      421 tgcagtgcag ccgctgttgc ataaaatttc gctctgcgtc gagtgtttct taatcaataa
      481 ttccgctgat actccggaaa aacgcgagtc tgttctcatg cctgaaatgc ccacacccca
      541 cgcaaaaaca attttttgct gatttttctt tataaataga gagttatgac aaattagttc
      601 ttcttgctct ctttgtgata tttaaaaaag cggtgtcggc gcggtgttgt agctgcgcca
      661 acaccgcttt ttaggggtgg tactgactat tttcataaaa aacatcattt tatattaggg
      721 gtgctgctag cggcgcggtg tgttttttta caggacgccc ctgggggcgc tgctaggggt
      781 gtctgttcag atatgtagca atgagtcagg gattcacgaa ctgaaaatgt ccgaaaatgt
      841 gtctcatggg caccgtatgc gcccaaaccg atcgccattc actttcatgc atagctatgc
      901 agtgagctga aagcgatcct gacgcatttt tccggttttc gcttggtaaa acatctcttt
      961 ttgcggtgtt cgcggcagaa tcgcgttcag cgcgttttag cggtgcgcgt aatgcgacgt
     1021 tatggtaaat gctgtgattt acgggatggt tttatctatg ttgatatcaa taccacctcc
     1081 tatggaggtg gcttacaccg atttttcctt gttgtctgtt tcagaacaga tagaggcttt
     1141 tttggcaatt atccaaccac tattgatagt gtttattact acttagataa ctgagccttt
     1201 actgtatctg cccctgcaag caggaaccca acagttaaga accaccccca gccattgata
     1261 ccttttagcg ctagaattgc tgcgctaatg atggctgcta tagggactat ctggtacaga
     1321 aaatacttca tataacttac cccgaggttt tgctttttta tgagacccta aagcatcgat
     1381 actgtcactt tgatgcttta aggaccttct tagtgataat cctcgagatc cacgtcaaag
     1441 acgttaggtt cttcgagctt aaacactacg cgccagtttc cattgacctc taacgaatac
     1501 tctccttgac ggtctccctt caatggatgg cagcgcaatg cctttaaatc catcatatcc
     1561 tgcaagttcg tcgctgtgct taaggctgtt aggatcagct tcagacgttg agtaaactgt
     1621 tgcggaattc ctgcgttgtt acctgtttcg gcaaacttac gcaaaccttt atgcttgata
     1681 tttatgatca ttaatcacca tcccatattt gtttagatag aaacatcgca gtttattttt
     1741 ccggttcatc cgtatttcca ttgcaaaccc cttgctctat ttgaataaat gacgtaggtt
     1801 ttacttaacc aagtcgcatt ttaatctgtt gacattggtt tgatgcgctg tacaatgccg
     1861 gggccttgaa tttcacatca agtacccaca tcataaggaa tgattagacg caccaatgtg
     1921 catcataatg tcattctttt ttttagtcaa caatgagttg cgttatgcgc aatgcgcaat
     1981 ctgaaaacgc aataggccta tctttttaaa actaatatag gcttcagagg aaggtatgat
     2041 taatcttgtc gatccagtac atccaggggt ttttatccgt gagatgttta tggaaccgtt
     2101 tgaaatatca gccgcggacc tgtctgaaaa actgcatgta tcaccttcca ctctaagtag
     2161 agtgctgaat ggaaaagctg atttaagtgt tgagatggca ttaaggcttt ctcgagttct
     2221 tggtcgcagt gccgagtcgt ggataaacat gcagactcaa tacagtctta acgcgagcag
     2281 gaagtcacct aatgtcatta aggaattaga tgaattggtt cctatatttt gagaaaagaa
     2341 aaggccgcta tatgcggcct ttttctgtct tgactctcag gcaactggat cgtatcgttt
     2401 ctcgtaaacg aaaaaaccac ccggcaaggt ggttttttcg aaggttcagt aaccggcaag
     2461 tttctgaacc agggtaactg gcttggagga gcgcagtcac caaaacttgt tctttcagtt
     2521 tagccttaac tggcgcataa cttcaagact aactcctcta aatcagttac cagtggctgc
     2581 tgccaatggt gttttgcatg tctttccggg ttggactcaa gatgatagtt accggataag
     2641 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2701 tacactgaac cgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     2761 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2821 ccaccagggg gaaacgtctg gtatctttaa gtcctgtcgg gtttcgccac ctctgacttg
     2881 agcgtcgatt tctgtgatgc tcgtcagggg ggcggagcct atggaaaaac gcctgcggcg
     2941 cggccttctt cctgttcttg gtcttttgct cacatgttct ttccggcctt atcccctgat
     3001 tctgtagata accgtgctgc cgtttttgtg taagtcagta ccgctcgccg cagccgaacg
     3061 accgagcgta gcgagtcagt gagcgaggaa gcggaaaagc gcctggacgc gcattttctc
     3121 cttacgcatc tgtgcggaat ttcacacccg gcatggggca cttttcatac aatctgcact
     3181 gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcagggctt
     3241 cgccccgaca cccgctg
//
