LOCUS       NZ_CP104672             4074 bp    DNA     circular CON 09-OCT-2023
DEFINITION  Escherichia coli strain 2021CK-00607 plasmid unnamed7, complete
            sequence.
ACCESSION   NZ_CP104672
VERSION     NZ_CP104672.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17815181
            Assembly: GCF_025398375.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Sangster,A., Hergert,J., Hirschi,B., Casey,R., Wagner,J., Young,E.L.
            and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-SEP-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 138.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/09/2023 03:50:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,322
            CDSs (total)                      :: 5,202
            Genes (coding)                    :: 4,979
            CDSs (with protein)               :: 4,979
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 223
            CDSs (without protein)            :: 223
            Pseudo Genes (ambiguous residues) :: 0 of 223
            Pseudo Genes (frameshifted)       :: 81 of 223
            Pseudo Genes (incomplete)         :: 151 of 223
            Pseudo Genes (internal stop)      :: 44 of 223
            Pseudo Genes (multiple problems)  :: 49 of 223
            Pseudo Genes (short protein)      :: 4 of 223
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104672.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2021CK-00607"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed7"
                     /geo_loc_name="USA"
                     /collection_date="2021-01-11"
     gene            join(3023..4074,1..1108)
                     /gene="mobQ"
                     /locus_tag="N5914_RS26475"
                     /old_locus_tag="N5914_26485"
     CDS             join(3023..4074,1..1108)
                     /gene="mobQ"
                     /locus_tag="N5914_RS26475"
                     /old_locus_tag="N5914_26485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_016245161.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(1366..2058)
                     /locus_tag="N5914_RS26480"
                     /old_locus_tag="N5914_26490"
     CDS             complement(1366..2058)
                     /locus_tag="N5914_RS26480"
                     /old_locus_tag="N5914_26490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281403.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(2590..2844)
                     /gene="mobC"
                     /locus_tag="N5914_RS26485"
                     /old_locus_tag="N5914_26495"
     CDS             complement(2590..2844)
                     /gene="mobC"
                     /locus_tag="N5914_RS26485"
                     /old_locus_tag="N5914_26495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091747.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          complement(2846..3022)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          3023..3381
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          complement(1367..2058)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.821"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3..1105
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.457"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2937..4072
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.208"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ccagtcgctg agccagcaaa tggaaaaatt gcgacagcat taccggaagg tggagaaaca
       61 ggacattccc gcgagtcaga cggcatttga aaaacagtgg cagcagtggg gggcatccgg
      121 ccttgcagct ctcagagtcc agttgcttaa acgtgaagag gaattgcgcc ggaaggagca
      181 ggaggataca gaacgtagtc gcgcggagcg acgagcggaa cagctcagga aaaatgacaa
      241 tgccgttatg gattcagggg tactgagcgc agtcgttacg gggtatggcg aggcccccat
      301 gcctggtaac ggggaaatga cctgttacct tctgcttcac aaccggaacg gagaatacac
      361 gctctggggg aacgagctgg agaaataccg caccagaatc tttgagcgtg ttgatttaat
      421 gcgtgacagg agtggttata tctgtgaccg aagcgaaatc ggtcaacatc caccactgca
      481 acgtgtttat tcctctgcga cctttgagca acttctgacg caggtttgcc agacatggcc
      541 gcaatatacc cgcaatctcc gccagccgaa gacatggccg gagtctttct gtcttggaga
      601 agaccgccag ccagccatgc catcactggc agcaaggaag gtggatttta cgcagggacg
      661 tctgccaccg acgctgatgc cggtaatgag ttctgtcgac cgggaaaccc gccagcttca
      721 gcttttgctg gtcatgggcg tggatgactc tctgggcggt gttgtccggc ttaatggtac
      781 gctgtatccc gcgtttgccg ttccctcagc cgataacagc cagctggtca tcagtgcact
      841 gaccgataag gggttacggt atgcagggta tggtgttgcc gtaaatcacg atgcagacag
      901 ccacatatca cctgcgccgg aacttatgga gtttcatctg aaaacccgtg aggcaccgct
      961 ttttgctgcc gtcaacacac cagaaaaaca gccggatcat ctgttccgct cgctgggttt
     1021 caatcgtacc tgggacgaat ggcggcgcga ggaagatgca agaacacaca ccaccgagag
     1081 gagacatgat cgcggttggt cacagtgaat gtgaacaata ttttgttttg tttaacatcg
     1141 tgttatgttc ttcgatgttc ttcgatgttc atcgacaggg atgaatgcaa gaagcacact
     1201 cacataatgc aagcatagtt ttgtttttcc gcccccaaaa attgggggtt cttttttcgc
     1261 tgcatcagag cgtttttgac gcaatttttc acttcaatag taccttcgca ctactaacac
     1321 aaaaaaaacg cagcagggag cgttttaggt gccttaaaat cgattttaga tcgtcatctt
     1381 gatctgttta tcttccttga agcgaaaatg cagggctaca accgtgcgcc cctttttgat
     1441 cgtctcaacc gttacagcca ggtcgctttt tttgttcagt tcgtcaacac agggttttat
     1501 tagtatttta tttaagtctc tgaattgttt gtatttattt tcaatcccta acattgaacg
     1561 aaagtcatta agagcaataa ttctctctcc cgtactgcgg aattgctgga ggaactcgta
     1621 tattcttata ctgtaactac ttgataaacc tgatatgttt tttataacaa cgcgcgtgaa
     1681 ttgccccttc agttgcgtca gataaggcat taccgcgtca gaaaaggtta ttcgagcctt
     1741 ggcctcacct ttagcgtact gtgcccgata ctggatccag cggaactcct cacgcttctc
     1801 gtcattcttg agaatgatcg acctgtccca taaacgatcg atcgcttcct gtaactgaac
     1861 ctcggcattt ttccgcccca tgtcagggaa tgaatcaaaa tattccgcag cggtaatggt
     1921 cattgtcttt tgcaattcag catcagcacc agatttcaat cgactgatac acagcaacag
     1981 aaaacgttgt tcctgtaggg tcaacttgta gctggcctca accaaagcat tcgacttgta
     2041 ggcaatcaag tccgacatac aaagatcccg ttcaaatgtg gtgatgcttt aatgtgcccc
     2101 acaaataaga aatgtggaat gttttttttt atatcctcac atttctgtga ataacacctt
     2161 aaattcctcc acctttacct taaattcctc cacctaaaca ccttaaattc ctccaccttt
     2221 accttaaatt cctccacata taccactata acatattgat tttattaatg aatttcaccc
     2281 ctaaaacaag tattaaaaac aagatcttaa aaacaagatc taaaaactta acggcgagcc
     2341 tgtggataac ttggggggat gaaaacaaaa aaccaataaa aacaatgtgt tgcaagaaag
     2401 cagaattgca agttccgaat actccccttc caaccctgtg gaaatccagt attcatgcgg
     2461 gttcagcgca tatcacctgc cgaaaaaccg tgcgtatgtg ctcaacctgg cgacggattc
     2521 acttccgctt cctcgcccac tcgctcgcta acgctcggtc gctggggctg cggcgagcgg
     2581 acgatacaat cactgtcctg gcagcgcctc cagctcgtta atcagcggca gtatgcgcgt
     2641 cacatcagcc tgtcgtgtca cctctgcctt caggaactgg agtaaccagg cacgcaactg
     2701 cacctcctcc ggctttctga cctttgccag caatgctgca cccacaacaa ccttctgcgc
     2761 tgtgtccagg ctccggctct tcgccttcag gcgctgtaat ctcgcctcag catcagcaat
     2821 cttctgttcg agcgttctgc tcatgatgta actccgtgcg cgatgaaaaa tcgcatttta
     2881 gcgcgtcact ggtagtttaa aaactgagct ggcataatgc acggcaaatc acgaagtgcg
     2941 cacttataca atctccactc cgtttcgatt gtgtgcgccc tgcggggctg aaagaaaact
     3001 acaaaaaggc attacggcag aaatggcgat ttatcatctc agcatgaaaa ttatctccag
     3061 aagcagcggc tacagcgccg tagcttccgc tgcctatcgt tccggttcac taatgcttga
     3121 tgagcgtacc ggactgactc acgactacac ccgcaaaagc ggtgtggctg aagctgtgat
     3181 tctgaccccc gcgacagccc ctgcctggtg tacgaaccgt gccgaactct ggaatgccgt
     3241 tgagaaggcc gaacgccgga agaactcaca acttgcgcgg gaaattgaac tcgctatccc
     3301 ccgtgaactg ccgcaggatg ccgcacggga aaccgttctc gcctttgtca gagaaaactt
     3361 tgtcagtcag ggcatgattg ctgatgtggc gttccatcac atggataaaa caaatcccca
     3421 tgcgcacatc atgctgacca cgagggctgt cggacctgct gggttcggcg gaaaggtcag
     3481 ggactggaac gaccggacac atgccgagac gtggcgtgca tcatgggctg accatgcaaa
     3541 cagagcactg gcgaacgccg gttatcagga agagatagac catcgttcat acgagcgaca
     3601 ggggcttgag aaaacgcctg gtattcacct cggaaagtct gcctgtgcga tggaaacacg
     3661 cggtatcgaa acagaacgcg gcgaacagaa tcgtctgatt aacaggctta acctggaaat
     3721 acagatttcc cgcactgaac tcaggaacag cgggctgaca gagcctgccc gccgtgtggc
     3781 tgaccttctg aatatcgtgc ttcctgacaa cgcaacttcg tacacactac gtgacatcat
     3841 cgaagcgttg ccgaaggaca gtaatgcggc atggaaactc accagtaatg ccatgtcgat
     3901 gatggcagat atgcaggcga cgcgcagacg ctgggaagag ctgaacaccg aacgaaagga
     3961 agcgatgagc catgctgcca gcctgaaaaa atcatcgccg ttttcgtccg gtttttcccg
     4021 tattcccctg atgcaatggg cggcaccgga ataccgtagc gaacagggaa aaat
//
