LOCUS       NZ_CP104671             4362 bp    DNA     circular CON 09-OCT-2023
DEFINITION  Escherichia coli strain 2021CK-00607 plasmid unnamed6, complete
            sequence.
ACCESSION   NZ_CP104671
VERSION     NZ_CP104671.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17815181
            Assembly: GCF_025398375.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Sangster,A., Hergert,J., Hirschi,B., Casey,R., Wagner,J., Young,E.L.
            and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-SEP-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 138.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/09/2023 03:50:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,322
            CDSs (total)                      :: 5,202
            Genes (coding)                    :: 4,979
            CDSs (with protein)               :: 4,979
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 223
            CDSs (without protein)            :: 223
            Pseudo Genes (ambiguous residues) :: 0 of 223
            Pseudo Genes (frameshifted)       :: 81 of 223
            Pseudo Genes (incomplete)         :: 151 of 223
            Pseudo Genes (internal stop)      :: 44 of 223
            Pseudo Genes (multiple problems)  :: 49 of 223
            Pseudo Genes (short protein)      :: 4 of 223
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104671.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2021CK-00607"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed6"
                     /geo_loc_name="USA"
                     /collection_date="2021-01-11"
     gene            complement(join(3503..4362,1..619))
                     /locus_tag="N5914_RS26445"
                     /old_locus_tag="N5914_26455"
     CDS             complement(join(3503..4362,1..619))
                     /locus_tag="N5914_RS26445"
                     /old_locus_tag="N5914_26455"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_220414910.1"
                     /translation="MKGSVAKTGKSFKNRVDYILKDDHDFICSNMASDYNNVSDLTDEF
                     KAVSSFRQDIKKPVFHAFLSLPKNEHLTDEQWQEIAKDYLKEMNINIEKHQYICVRHND
                     TDQDHIHIVANRVGLDGSVWLGQHSAFNTIAACERLEIKHNLTITQGLKGQKSDVSAPT
                     KNEIEMALRTGEKPARLVLQASLQAAMTGKPDLETFVERLQAVGIEPQFNVASSTGNVA
                     GCSFSVGDVAFKGSQLGKKFSWNTIKNKVKYDKNRDDELVRRFSARKADNENSIGRSFV
                     ESNINDSRTSDRINDDIIGVGQYGIKPDTTDTYEGEQLVRSNNHSFKLSEKYNRPASHK
                     VRRTTQKAAQILNRVKKQYNKEPIKTYNKNGLNSWRDKSDSSSISRILDHAETATTATN
                     NFRLKSGSRFNSRATKIEEIRERLGIKKPIIQPKKIVKEEIKKKPLGLSIDENRFKSKL
                     KEALKKEKPKNETTPTQLQQEDDQHLKPRGFKPR"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(616..987)
                     /gene="mobC"
                     /locus_tag="N5914_RS26450"
                     /old_locus_tag="N5914_26460"
     CDS             complement(616..987)
                     /gene="mobC"
                     /locus_tag="N5914_RS26450"
                     /old_locus_tag="N5914_26460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_113282474.1"
                     /translation="MDTDRKRGRKGLNHAERRDISIQFRVNKAEFDYLKELVEASGLER
                     GHYIRKEILNSEPVIKRKMPEFEQSLYREIQQQGNNINQIAKKLNTNEDFKQSLAAEAN
                     FAIKQLMKRILKACEVYTV"
                     /name="mobC"
     gene            complement(974..1234)
                     /locus_tag="N5914_RS26455"
                     /old_locus_tag="N5914_26465"
     CDS             complement(974..1234)
                     /locus_tag="N5914_RS26455"
                     /old_locus_tag="N5914_26465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063617446.1"
                     /translation="MNENDIDQSLLELLARYFYYIDPNEENTAFLEANKDEQDLCYFVA
                     YRYIKENKTQDLIDALKDQKDEDYIKAMKDYVYGGGKYGYR"
                     /name="hypothetical protein"
     gene            complement(1852..2076)
                     /locus_tag="N5914_RS26460"
                     /old_locus_tag="N5914_26470"
     CDS             complement(1852..2076)
                     /locus_tag="N5914_RS26460"
                     /old_locus_tag="N5914_26470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_113282475.1"
                     /translation="MHEQKDKKRRLGFMVGKSNATLPADEQTEAELDQEVLKLFYPNQC
                     TKDTIERSEAGTDVFTASGSDDLFKKLKL"
                     /name="hypothetical protein"
     gene            2374..3225
                     /locus_tag="N5914_RS26465"
                     /old_locus_tag="N5914_26475"
     CDS             2374..3225
                     /locus_tag="N5914_RS26465"
                     /old_locus_tag="N5914_26475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_113282473.1"
                     /translation="MFNIENNREILKSQIPLKPYATNDLEFGLTIQNKNKALDMLYLQV
                     NHPLYLHTMIFDLDKENCFYEFENAHLPIPSFITKSPESGRCHYGYMLNAPISSTEKSR
                     QKPVKFARALYYNMATRLGADLSYAGLITKNPMNSHWSPFWSGADLYELNDLADCFDDL
                     EEPKKRENTEFAFGRNVEMFDTIRQWAYKNVLKYQSESSFNDFHNELLLKCQMHNAYLN
                     ADDLLPYNEIKATAKSIAKFCWKEFSEEKLNKIQSKKQSYRGKKNKGIVKSKTRKFLEA
                     IK"
                     /name="replication initiation protein"
     gene            3222..3494
                     /locus_tag="N5914_RS26470"
                     /old_locus_tag="N5914_26480"
     CDS             3222..3494
                     /locus_tag="N5914_RS26470"
                     /old_locus_tag="N5914_26480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein"
                     /protein_id="WP_072205318.1"
                     /translation="MKVKNRKRELTAKEYAEQYEISVRTVKRYFSQDREDYEKEAKQRR
                     LKAFVLRESGMKWADVGIALGTTKHGAIALYKRYQQIDAPTTKEA"
                     /name="plasmid replication protein"
ORIGIN
        1 ctacggcctg cagacgttct acgaacgtct caaggtctgg tttacctgtc atagctgctt
       61 gcagagaggc ttgaagaact aaacgggctg gcttttctcc tgtcctcaat gccatctcga
      121 tctcgttctt ggttggtgct gatacgtctg atttttgacc tttaagcccc tgtgtaatgg
      181 ttaagttgtg ctttatctca agacgttcac aagctgctat tgtgttaaat gctgagtgct
      241 gcccaagcca tactgaaccg tctaaaccga ctctatttgc tactatatgg atatggtctt
      301 gatctgtatc gttatgacgc acacaaatat attgatgttt ttctatatta atattcattt
      361 ctttcaaata gtcttttgct atctcttgcc attgttcatc tgttaaatgt tcattcttgg
      421 gaagagataa aaaagcgtga aaaactggtt tttttatatc ttgcctaaaa ctgcttacag
      481 ctttaaactc atcagttaaa tcactaacat tattataatc acttgccata ttgctacata
      541 taaaatcgtg gtcatcttta agaatgtaat caactctatt tttaaaagac ttacccgtct
      601 tagctacgct tcctttcata ctgtataaac ctcgcaagct tttaatattc ttttcataag
      661 ttgctttatg gcaaagttag cttctgcggc taaagattgt ttaaagtctt cattcgtatt
      721 tagcttttta gcaatctgat ttatattatt accttgctgt tgtatttctc tgtataatga
      781 ttgctcaaac tcaggcattt tcctttttat aacgggttca gagtttaaaa tctcttttcg
      841 gatgtaatga cctctttcaa gccctgatgc ttccacaagc tcttttaaat aatcaaactc
      901 tgctttgttt actctaaact gtatagatat atctctgcgt tctgcgtgat ttaaaccctt
      961 gcggcctctt tttctatctg tatccatatt taccacctcc ataaacataa tctttcattg
     1021 ctttgatata atcttcatct ttttgatctt tcaaagcatc aataagatct tgagttttat
     1081 tttctttaat atagcgataa gctacaaaat aacataaatc ttgttcatct ttatttgctt
     1141 caagaaatgc tgtattttct tcatttgggt ctatataata aaaatatctt gctaatagtt
     1201 ctagcaagga ctgatctata tcattctcat tcattttaaa cactcctata tatatttata
     1261 atattcaatg taaaactatg gtttaccgcc tcccctttga taaggggaat aggcggaagg
     1321 gtagatcttt ttcaacccta cgagcaaagc gagatagggc aatccgaagg attgggtata
     1381 ataccggcta cgggattaca ccttgcctca gtttccgaaa agctaaaaaa aagctgaact
     1441 caacctatat ttaaataaat aaccgatcta aaaagtttgt caatatagcg gctacataaa
     1501 taaatacact gcacaacaaa taaaatgcaa ataaaaaata gggtaaataa tatattgtct
     1561 tgatgggtag ctgtgcgtaa aaaatgctgc tgctacgtga aatctggaaa agttcgtgac
     1621 ctcttggtag tcagatttca ccagatgcaa agggatgggg ttgatcggcg gcgacagaaa
     1681 cgagtaaaac ttgcttttgc tcgtttgctg gcgtctatgg gtttaaaatg ctcataaaat
     1741 caacagacta tagtatatat tagtatatac tgcatcgagg gttggccttc tctcaccgtt
     1801 ccgggtcggt ttgttctcaa gtggtgaaga tggccagctc tcccctaccc tctacagctt
     1861 caattttttg aacaggtcat cagaaccgga ggccgtgaaa acgtctgtac cagcttctga
     1921 gcgttctatg gtgtctttgg tgcattggtt aggataaaac agcttcagga cttcctgatc
     1981 aagctctgct tctgtttgtt cgtctgctgg caatgttgca ttgctcttac caaccataaa
     2041 acctaaccgc cgtttcttgt ccttctgttc gtgcatgatg tacctcccca gcacagccag
     2101 atcctgcata gagtttagta tacgttacag tgtgacttac tctgtagatg gccttcggcc
     2161 tgtgcttcgc ggttgtatgg ttttactgaa aactccgtct aatacctgat attggttaat
     2221 ggcacacata acaacttttt ttgatggatc tattgacaag tttttttggt atagtaaaac
     2281 tgtaagagat tataaataaa agaaaagccc gcgtttcttg gaaagtttgg cgaccaacaa
     2341 gaaacggggc attacagaaa ggattactgc taaatgttca atatagaaaa taacagagag
     2401 atattaaaaa gtcaaatacc tttaaagcca tatgctacaa atgatttaga gtttggattg
     2461 actattcaaa ataagaataa agctttagat atgctttatt tacaagtaaa tcacccgttg
     2521 tatcttcata caatgatatt tgatttagac aaagaaaact gcttttatga gtttgaaaat
     2581 gctcatttgc ctataccgtc ttttataact aaatcaccgg aatctggtag atgtcattac
     2641 ggttatatgc ttaatgctcc gatctcatca acagagaaaa gcagacaaaa gccagtaaag
     2701 tttgctagag cattatatta caacatggct actcgtttag gtgctgatct tagttatgcc
     2761 ggactgatta ctaaaaatcc tatgaatagc cactggtcgc cgttctggtc aggtgctgat
     2821 ttatatgagt taaatgattt agctgattgt tttgatgatt tagaagaacc aaagaaaaga
     2881 gaaaatacag agtttgcttt tggtcgtaat gttgaaatgt tcgacaccat aaggcagtgg
     2941 gcttataaaa atgtgctaaa ataccagagt gaaagctcat ttaatgattt ccacaatgaa
     3001 ctattgctaa agtgtcaaat gcataatgca tatttaaatg ctgatgattt actgccatat
     3061 aatgagataa aagccacagc aaaaagcatt gcaaagtttt gttggaaaga attcagcgaa
     3121 gaaaaactca acaagataca gagtaaaaaa cagtcttacc gtggaaagaa aaacaaagga
     3181 atagtaaaaa gtaaaactcg taagtttttg gaggccataa aatgaaagtt aaaaatagaa
     3241 aaagagaact taccgcaaaa gagtatgctg aacagtatga gattagcgtt agaacagtaa
     3301 aaagatattt ctctcaagat cgtgaagact atgagaaaga agcaaaacaa agacgattaa
     3361 aagcctttgt attacgtgaa agcgggatga aatgggcaga tgtcggtatt gctcttggaa
     3421 ctacaaaaca cggggctata gctctttata aacgttatca acaaatagac gcaccaacaa
     3481 caaaagaggc ttaaagcctc ttttatcttg gtttaaaacc acgcggtttt aaatgctgat
     3541 cgtcttcctg ctgtagttgt gttggtgttg tttcattttt tggtttttct tttttaagag
     3601 cttcttttaa cttcgattta aatctatttt catcaatact caaccccaaa ggctttttct
     3661 ttatttcttc tttaactatt ttctttggct gaataatagg tttttttata cctaaccttt
     3721 ctcttatctc ttcgatctta gttgctctac tattgaaacg gctaccgctc ttgagtctaa
     3781 agttattcgt ggctgttgtg gctgtttctg catgatcaag tatcctactg atagaactgc
     3841 tatcagattt atcacgccaa ctattgaggc catttttatt ataagttttg attggttctt
     3901 tattgtattg ttttttaact ctgttgagta tttgtgcagc tttttgtgtt gttcttctaa
     3961 ctttatggct tgctggtcta ttatattttt cgctgagttt aaagctgtgg ttgttgcttc
     4021 taacgagttg ttcaccctcg taagtgtctg ttgtgtctgg tttaatgccg tactgaccga
     4081 ctccgataat atcatcattg attctgtcag aggttctact gtcattgata tttgattcaa
     4141 caaatgatcg tccaatacta ttttcgttat ctgcttttct tgctgagaat cttctaacga
     4201 gttcatcatc tctatttttg tcatatttca ccttgttttt tatagtgttc caactaaatt
     4261 ttttacccaa ctgtgagcct ttaaatgcca catctcccac actgaaagaa caacccgcca
     4321 cgttgccagt gctgctggca acattgaact ggggttcaat gc
//
