LOCUS       NZ_CP104663             9294 bp    DNA     circular CON 09-OCT-2023
DEFINITION  Klebsiella pneumoniae strain 2021CK-01729 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP104663
VERSION     NZ_CP104663.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN24145033
            Assembly: GCF_025398355.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Sangster,A., Hergert,J., Hirschi,B., Casey,R., Wagner,J., Young,E.L.
            and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-SEP-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 349.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/09/2023 03:29:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,527
            CDSs (total)                      :: 5,406
            Genes (coding)                    :: 5,283
            CDSs (with protein)               :: 5,283
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 49 of 123
            Pseudo Genes (incomplete)         :: 66 of 123
            Pseudo Genes (internal stop)      :: 30 of 123
            Pseudo Genes (multiple problems)  :: 20 of 123
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104663.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2021CK-01729"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA"
                     /collection_date="2021-11-22"
     gene            complement(join(8674..9294,1..111))
                     /locus_tag="N4T33_RS27545"
                     /old_locus_tag="N4T33_27550"
     CDS             complement(join(8674..9294,1..111))
                     /locus_tag="N4T33_RS27545"
                     /old_locus_tag="N4T33_27550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            complement(111..2075)
                     /locus_tag="N4T33_RS27550"
                     /old_locus_tag="N4T33_27555"
     CDS             complement(111..2075)
                     /locus_tag="N4T33_RS27550"
                     /old_locus_tag="N4T33_27555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(3556..3705)
                     /locus_tag="N4T33_RS27555"
                     /old_locus_tag="N4T33_27560"
     CDS             complement(3556..3705)
                     /locus_tag="N4T33_RS27555"
                     /old_locus_tag="N4T33_27560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(3790..4047)
                     /locus_tag="N4T33_RS27560"
                     /old_locus_tag="N4T33_27565"
     CDS             complement(3790..4047)
                     /locus_tag="N4T33_RS27560"
                     /old_locus_tag="N4T33_27565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(4057..5742)
                     /locus_tag="N4T33_RS27565"
                     /old_locus_tag="N4T33_27570"
     CDS             complement(4057..5742)
                     /locus_tag="N4T33_RS27565"
                     /old_locus_tag="N4T33_27570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            6145..6564
                     /locus_tag="N4T33_RS27570"
                     /old_locus_tag="N4T33_27575"
     CDS             6145..6564
                     /locus_tag="N4T33_RS27570"
                     /old_locus_tag="N4T33_27575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            6870..7133
                     /locus_tag="N4T33_RS27575"
                     /old_locus_tag="N4T33_27580"
     CDS             6870..7133
                     /locus_tag="N4T33_RS27575"
                     /old_locus_tag="N4T33_27580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            7123..7422
                     /locus_tag="N4T33_RS27580"
                     /old_locus_tag="N4T33_27585"
     CDS             7123..7422
                     /locus_tag="N4T33_RS27580"
                     /old_locus_tag="N4T33_27585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7484..7861)
                     /locus_tag="N4T33_RS27585"
                     /old_locus_tag="N4T33_27590"
     CDS             complement(7484..7861)
                     /locus_tag="N4T33_RS27585"
                     /old_locus_tag="N4T33_27590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            7930..8109
                     /locus_tag="N4T33_RS27590"
                     /old_locus_tag="N4T33_27595"
     CDS             7930..8109
                     /locus_tag="N4T33_RS27590"
                     /old_locus_tag="N4T33_27595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8137..8667)
                     /locus_tag="N4T33_RS27595"
                     /old_locus_tag="N4T33_27600"
     CDS             complement(8137..8667)
                     /locus_tag="N4T33_RS27595"
                     /old_locus_tag="N4T33_27600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     BGC             1..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2875..3453
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8678..9294)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="85.0"
                     /evalue="2.39e-134"
ORIGIN
        1 ctccgccgtg gaacacagct cttcccgcaa ccagcgcagg gtcagccggg ttttctccgc
       61 gttccgcgcc tgtcgtttcg cgtggctgac gttgtacagc tccagcgcca tcagtaaatc
      121 cccgcaaaat catcatccgc tttcggcccg ccgccgtcgg ccgggggccg gaactccgcc
      181 aggggatcgg ttttcgccag caccgggaaa cgctgcaacg ggatttcccg gcgctcaacc
      241 cgcagcggac tgctgaacgc catccgggcc acacccagcc cggtcatgac cgcgaacccg
      301 tccggcaggt gttgcagggt gttcacgtca aacagcggcc gcgcctcctt ctggatgaag
      361 ctgtccccgc tgatatgctc cacgttcccc gcctcccggc tggcacggcg gcgctccacg
      421 tccaccctga tttccccggt ctgccccgcc gcccactgcg cggactccag gctggccgcc
      481 cggtaaaacc agcggatcgg cgtgttatcc agtaccgtgg ttttcacaaa ttcagcgggc
      541 agatcctgcc cgcactgccc gaaatcgccc agcgactggt gcgcgagcaa cagattgcag
      601 tcccggctgc gcagcgtccc cagtgcgttc agtacatact tcgacagcag atatttaatt
      661 tcatcgagca tcacgctgac gtgtggccac tcccggaact cgtcccgcgc aatgatgatt
      721 tgcgcgcagc gggcaaacag cattttttgc acacggatca ccgcctcgtc gtccatcgag
      781 ccgataacgt acaggcagcc gccgccgttg ataatccccg ccacatcgat gccactgtcc
      841 gtctgcagga cgctcaggtc agcgacgttt tccagcctag tgatcaaccc cttcacgtcc
      901 tttttcaggg cctccggcag cgcgtacgcc gccgccagca cctgctgaat gtttgccccc
      961 tgcggaaact gctccgcaat cagcttcgcc gctttctgct cgctgatgcg gtagtggtcc
     1021 gccacgtcgc cggtctcgcc caggttaagc ccggcaatca gcaggttttt cagcgcgtac
     1081 tggtcgagat cgcggaacag attcagctgc gccggtttcc ccggccgcag atccagcagg
     1141 gtgaagtccg gacagtgcgc ccggaagacg tgcggcgccc atgcgtcccc tttggggtcg
     1201 aagtacacca ccgcgtcccc gaaatcgcgt acgcagcgaa tgagcgcgtt ggtggccatc
     1261 acgctcttgc cgctcccggg caggccgagg atctgaatgt tggtcttgcg ccaggtatca
     1321 cgcggaacca gcaccggctt gccctgctca tccttaccca gaaacaccgc ctgctcctgc
     1381 cgggcctgct ccgaggaaaa atcggccagc gcattaaccg gtaccggctc cccgaaatgc
     1441 aggtggcgca cgtcggtcat ctctcctgtc cgctggctgc tgcgccggct ctcctgattc
     1501 agtttcgcgt tcaccgcctc gtcagcagcc gcaaaatgac cgtcaagcag caggaacgcc
     1561 agcagccgcg ccaacacaat cccgacagcc agcccgcccg cgcaccaggc aatcccgaac
     1621 aggttagccg gctgcagcag gtaataatct gcgtttgcgt agaggtccgg acgcaggacg
     1681 gtttctcccc agctctcgat aaacacggcg acaaccagcc cccccagaag ggccagaccg
     1741 gccgcgccgg tcagccagta cgcgtagttc cagacatgtc gacgtgccct gaccatccgc
     1801 ccgggcgaat ccagcgaggc cagcaccgaa aatggcgctg gccgtttatt atcgcgctta
     1861 tagcgatcgg catactcccg tcgctcctta tgactcagcc ccacaaaacg catccgtccg
     1921 gcccaaagtt cagccagacc gaaaaacagg ccgataacta ggaaagcccc ggccagaccg
     1981 taatactcct ggtacagcca gctgacccac ggataaatct gccgggccag ctccggaatg
     2041 tgccacttcc aggcaagcga atccgtatta aacatgttct ctctccttca gacgatggtt
     2101 tgccggcgtg atgtgcagcc cccgctgcac aagccgcgga catgccccaa ggcatggcgg
     2161 cggcggtcac gtccgtggtg attgcgttca gctttcacca cggaggccag cctctttccg
     2221 ccccatgcgg aaaaggcgca gccccggagg aacaacgagc acgccccagc gtgtgagagg
     2281 aaaaggcggc accccagtgc ggtcttttcc ggtgttccgg cccccgtcag agttcgccat
     2341 agcgagcggc tgaccacccg caaaatcagg agcagctttg ctgcttcgcc aggtgcgtcc
     2401 agcaccgtgg atttttgtgg gtggtcaggc gtgagcctga cgggggccgg gtgaatttct
     2461 ggcgtggccg gcgtccagcc ggacggagcc agaaagaggg ggagagaccg tgcgtcggtg
     2521 gcgtccagcc agcgtaagca ggggatatcc ccctgaggtc agccacgccc tcgcggggat
     2581 gacctgtatt tctccgtaag gagaaaaaag caacagagat aggttaacgt aaaatgttca
     2641 gcgaaaaacc gatatgattc acctatcctg gtaatttatt atcagggaat ataaagactt
     2701 aatcagggga caatatgcca catcagcgcg tccggtgtcc gtcttgtagg tgccggggaa
     2761 attgcgcctc ctcgctcact cggtcgctac gctccggccg tgagactgcg gcgggcgctg
     2821 cggacacatg caaagttacc cacagattcc gtgaataagc aggggactaa catgtgaggc
     2881 aaaacggcaa ggccacgcag gtggcgtttt tccataggct ccgccctcct gactgagttc
     2941 acataaacag acgctttttc ggtgcatctg tgggagccgg tggctcaacc atgaatcagt
     3001 caggagtggc gaaacccgac aggacttaaa gatcatcacc gtttccgatg aatcgctccc
     3061 tcttgcgctc tcctgttcca accctgccgt ttcccggata cctgttccgc ctttctccct
     3121 tgcgggaagt gtggcgcttt ctcatagctc acactctggt atctcggctc ggtgtaggtc
     3181 gttcgctcca agctgggctg taagcaagaa ccccccgttc agcccgactg ctgcgcctta
     3241 tccggtaact gttcacttga gtccaacccg gaaaagcacg caaaacgcca ctggcagcag
     3301 ccattggtaa ctgggagttc gcagaggatt tgtttagcta aacacgcggt tgctcttgaa
     3361 gtgtgcgcca aagtccggct acactggaag gacagatttg gttgctgtgc tctgcgaaag
     3421 ccagttacca cggttaagca gttccccaac tgacttaacc ttcgatcaaa ccacctcccc
     3481 aggtggtttt ttcgtttaca gggcaaaaga ttacgcgcag aaaaaaagga tctcaagaag
     3541 atcctttgat cttttctact gaaccgcgat ccccgtcagt tcagaggagg acgatggtgc
     3601 caccgttcca ccctgaacat cccggatata gtttgcctga catgcgacca gaagaaagcc
     3661 tgaaaaaatc aatataaaaa gaaaaacaac ctttaccttt ttcatatacc ttccctgtat
     3721 ttaaaaaccc cggtattcat ccctgaacac cggaagtctg tatttcaaat catccttgat
     3781 aacttttgct taccagttac catcccggta aacaaaggat acaaagtaat caaatttact
     3841 aatatcgatt acatttttaa agtgtggctg taatatcgaa acccatgatt tttcaacatc
     3901 aaaccaacca ttattaatat tatcctttaa aggcatcccc agactttcaa tgacagaacc
     3961 atcatcaccg aagtcttttg agtattcacc acctttaaac tcttcggttt tcttatcaaa
     4021 ccaatgaata tgtaatttaa gccccatatt tacctcttaa agatattttt taatgtttcg
     4081 ttttggatcc ggccctttaa cctgcttacc cgtttttgga tcgaatgccc cgaggtgttc
     4141 gccatcactg gcgcggtacc cttcaagctc accgtgctgg gaatcccatt cataaatttt
     4201 acgcttttta tcaccatacc atcgggcccg cttaccacca ccaccttgtt taggggtttt
     4261 aggtttaccc tctttcaact ctcccaaccc cttaatatct tcagtcttgg gatcaggaaa
     4321 ataatcatgg ccgtaatctt tagttccctt acgaggtttt ttcttttcct catttaattt
     4381 attttctgca tccttcgctt ttttctcctt ttgtttccgg ctctccatcg ccccggaaag
     4441 cgcagcctta tccctggata aggattcatt catttcgttt aaagatgtct gggcagcttt
     4501 taatttctca ttctgattct gaatatcctt atcaagatag cgaacctgat cctgccaccc
     4561 ggagccatat tcatgaggcg ggttataagt cacaaaatca tctttatttg cctctgcctc
     4621 ttttaccttt ttatcggctt cctgaacagc agcccttgca ccggggatcg ccttctctgt
     4681 cgatgcaatg cgactgttta aggtcgcaat atttttatct tcagcatcca gctcagcttt
     4741 cgctttatca tactctcttt tcagcccctc ttccgggtga gcagcgtccc atgcctgctg
     4801 gcgacgcttt tcttcctcca gacgctgttt tacctgtgcc ggagtaagaa catccgtcac
     4861 cgacacatag accggtttct gtccgctctc tttcgggaaa cgaataacgg cttcacggga
     4921 gttaccaccg gccgtaaaac cagccggacg tgaatcacct ttttcagcaa taatgccttt
     4981 tggcggggct ttcgcggccg gaacaccttt aggtacgctc acctgcagag acgggagacc
     5041 cggaatcgac acactgaata cccccggacg ttttgtcggt ttcgcatcca ccacaggaac
     5101 actcattggc cggccggtga caaccgcaat atgctgccgc tcgtccttca caatatccgc
     5161 cacacgttta gtgacgctga ccgtcgcctg gtccagcggc agggagctga ccggcgtctc
     5221 cgttaccgtg tctgccggca atgacgtcac gatttttgac atcattttcg gatcatcttt
     5281 tgctatctca gaaggcagca ctccgtaaat cgcaatcccc cacagaccaa acttaaacgg
     5341 acctttcagg gcagccagca catcagcaac agcggccgac aacgcatcgc cggaaacgga
     5401 taaagccagt ccttcagccc ccggagtggc caaggcaggc agaccgaagg ccatcgcggt
     5461 ggccgaagac tgtccgccac cagagctccc gctatgattg ccgctgttac cgccattacc
     5521 accgggttta ctcccgccgt taccaaactc ccccgggtta tagttatgaa tatccccgtt
     5581 aggtgtatgc gtcgtacccc agcccggccc gctgtttgga tccgtaccac cacctgatga
     5641 agggccacct ttacctgatg taccgttcac attcccactg gcctgaccac cattgtgtcc
     5701 cagacctgaa ttacccggac ctcgtccgtc tccgccactc atatactttt cctctttgac
     5761 ttttaaaaca ataaactaaa ataaaatact gtacatataa ccactggttt tatatacagt
     5821 ataaaaatta cgctgccttg tttttcatgt caagtctgta aatttgcgtt tttgtgaaaa
     5881 tgatcaaaaa aacagccact cacaccatgc gcaatcccct ccagatgcaa caaaaaccag
     5941 cacaaacggc gccgttctcc ggcctcagta tgttttactg aaatcattat atgagatacc
     6001 agaaagcccc ctccgggaca ggaaaagaaa cggggggtct ggttcgggtt gttcggtcac
     6061 tgaacacggt cagaaactga tgataagata gcctgcggat atataactca atgagaaaaa
     6121 agaattaaag gtaaggagac gctcatgaga cgttccggca gcaaactgtg tcttatcgcc
     6181 atgataacag cgacagcaac tctcactgcc tgtgctccaa agggcagcgt ggaacagcat
     6241 acccggcatt acgtatatgc ttcagatgac ggttttgatc ccaatttttc cacccagaaa
     6301 gctgacacaa cacgaatgat ggtgcctttt ttccggcagt tctgggatat gggcgcaaag
     6361 gataaagcga ccggaaaatc acggagtgat gtgcagcaac gcattcagca gtttcacagt
     6421 caggaatttt taaactcact ccaaggcaca acacaatttg cgggtaccga ttaccgcagc
     6481 aaagacctca caccgaaaaa atcaaggcta ctggctgaca ccatttcagc tgtttatctc
     6541 gatggctatg aaggcagaca gtaagtggat ttaccataac gcgtcattac gcgcaccgct
     6601 cagacgggct cagggcgttt ccggcggcta cgggataaaa caggcaacaa cccccccgga
     6661 aaactgccgg aaacgcgccc gataaatttt atcctaatgc atgcctatgc acgcaactgc
     6721 atggcatatg ttttgacgta aaatggatgc cgtaagccct tttttaccga ctttctgctt
     6781 catcagcctg actgacacga ccatattctt tcagaccgcc ccctgctaaa cttccttcat
     6841 attgccgcat taacccggga ggctccgcaa tgagcaacac tacacactac gaaaacgcca
     6901 actttcttcg ggaactggct gaaagcctgc cccgcatact gccggaaggt ggcccggaca
     6961 aagctgcgct gttgcagcga ctggctaacg aagaactggc ccaggcagag tatgaagatc
     7021 aggttcgcgc caaagtgacc gccgcccgtg ctgacacgcg tccgggtatg accacggaac
     7081 aactccgcca gcgcctgcat ggccgttatc aggaactgcg cgatgccgta tgacgtggaa
     7141 tggaagcagg gagccatcga ggatgtaacg acgctgtttg actatatcgc ggaaaattca
     7201 tccctgtggg atgcgcagaa cgttaccgac cgggttctcg ctgcggcgga caaactggcc
     7261 gacttcccgc gactctatga agtggactca cgctatggcg aggacgttcg ccggatcagt
     7321 ctgatgggac agcatgtcct ctatgatgtt gatgaccaga ccagaaaagt ccgggttctg
     7381 gccgtggtcg gccagcgcca gaatccccat attgttctct aataaccggt gacggggacg
     7441 caattcattg cgtggccgtc gccggttgga caagcacagc gcgtcagcgt tttccccacg
     7501 gccaccaccg gcgcggtgtt ccggccgggg tctctgctgt ctccggtttg tgttccagca
     7561 gtctcatgga ctgcatttcc tgccgtagcc cggccacctc ctccctgagt gcttttatct
     7621 ccgcgatcaa cgcgtcggag gccatttttt cagtctcacc cgacagtctc aactcgtccc
     7681 gctcgtgagt ctcatcctgt cttaactcac cgtacacccg gataagctcg ctggtatcca
     7741 ccacccggcc gccatcagct tccgtacggt atgatacgcg gcctttcgcc atatcccgat
     7801 acagggaact tcgggcttta cccgtcagtt ccgtcgcctg tctcaccgtc acccacgcca
     7861 ttgtctcacc ccgctatttt tgagacagta tacctgacag gcgcacacca tgcccggaag
     7921 gaaagaatca tgacaaaaca ggaaaaaaca gccctcaata tggctaaatt tattaaggag
     7981 cagtcgctgc tattgctcga taaactgaat gagctggata tggatacccc ggccgacatg
     8041 tgcgagcggc tacacgagga tgcggaaaca ctctggcagg tgatgcggga taagctcggg
     8101 gaggaataac agcaccggca tggccggtgc tggcatttat tttttgccga acggccacca
     8161 ccggcgggca ggtttgtcct ccggcagttc aggtaccggc acgttaaaat agcggctgtt
     8221 atattcccgc atcagctccg ctttcagctt ctcgttcacc gcgctttccc cgtgccgctc
     8281 actcaccacg tcaatcatcg cgttaatctg ctccggtgag cacttcccgg catcaaccac
     8341 ctgcacaccg ttctcgatgg cgaaccgctc caccttgcga tagaggtaat cacacagctt
     8401 cattcctgtg ggtttgttcc gttcccattg cggattttta ccagtgaaaa agtacgtggc
     8461 tttttcgtcg ctgttgagcc gctcgtcata catcccgatg gatttcatca tcgccagatg
     8521 ctgggcccgg tcgacaaaac agaaaacata cgccccggtc tgccaggcga gcaccaccat
     8581 atcttccaga tgctcgctgg tcggcatgat gcagcgctcg ccgttccggt cctccgcgtc
     8641 ttcaataaat accatgtaca gcgccataat ccctcactcc gttgtcataa atttaaagca
     8701 cgcaaacaac cgttcacgat ccagctctgc cgttttgcgg gcctcgccgg tctgtgggtt
     8761 cacgatttca tccaccgaca ggattgcccg ctgcaccctg acctgcgtcg cccggtccgg
     8821 gcagatgtac cagatttcat cgatgccgtt agctttgcgg ttgaacagat acccggacac
     8881 cacttcctga taccggcgac ggctcttgac ggtgcgctcc acctcaaacg ccaccgtttt
     8941 accgtccagc tccgccaggg cgtccggcac tttcatgcct ttaagactca gacggtgcag
     9001 aatgcgccag ccggtggccc ctctggcttc catatcaagg cgcagggatt gcacggccag
     9061 ccggtgcggc agatgtgctg ccgacaccct tgacggctga aacggaataa catccgccag
     9121 cggttcgtcc tcgtcgaacg agaacgccac gccatgcggc gtcagccccc acacgctgac
     9181 ctgacggccg tccaccggat atttggtccg gaccagcagc cctttcgcca ccaggctgtc
     9241 gaggaagcgg tagaccggct gcaccgccgc aatccccagc cgccgcgcaa ccag
//
