LOCUS       NZ_CP104626             2606 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Escherichia coli strain AHM9C68I plasmid pHNAH968-7, complete
            sequence.
ACCESSION   NZ_CP104626
VERSION     NZ_CP104626.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30667243
            Assembly: GCF_030169475.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   He,W., Gao,M., Lv,L., Wang,J., Cai,Z., Bai,Y., Gao,X., Gao,G.,
            Pu,W., Jiao,Y., Wan,M., Song,Q., Chen,S. and Liu,J.H.
  TITLE     Persistence and molecular epidemiology of blaNDM-positive
            Gram-negative bacteria in three broiler farms: A longitudinal study
            (2015-2021)
  JOURNAL   J Hazard Mater 446, 130725 (2023)
   PUBMED   36630875
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   He,W., Gao,M., Lv,L. and Liu,J.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2022) College of Veterinary Medicine, South China
            Agricultural University, Wushan, Tianhe District, Guangzhou,
            Guangdong 510642, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030169475.1-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 05:22:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,917
            Genes (coding)                    :: 4,647
            CDSs (with protein)               :: 4,647
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 82 of 270
            Pseudo Genes (incomplete)         :: 192 of 270
            Pseudo Genes (internal stop)      :: 45 of 270
            Pseudo Genes (multiple problems)  :: 44 of 270
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104626.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="AHM9C68I"
                     /isolation_source="chicken feces"
                     /db_xref="taxon:562"
                     /plasmid="pHNAH968-7"
                     /geo_loc_name="China"
                     /collection_date="2019-12"
     gene            complement(join(2289..2606,1..393))
                     /locus_tag="N5859_RS25120"
                     /old_locus_tag="N5859_25035"
     CDS             complement(join(2289..2606,1..393))
                     /locus_tag="N5859_RS25120"
                     /old_locus_tag="N5859_25035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000967646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_284632981.1"
                     /translation="MLFWKKMPSLWIGNQIAEFSDLDTAKAIAALKIYLTFCLFCKESD
                     SGCRTVKLTFSEICETASMSRSLVNEGLKILYAKKLIKNVSQTERKKIYTVDVLGPHED
                     GWCKLPLKGVVGEDNKISAFQSMHNRYPFELLALQTYMYLLYARDNRNDYTLARKKTIC
                     TKLKCKLTDLNKAITYLIHIGLLDRVVKKAIVDKPLDLFHDSCYFYMKTGSERALTYRK
                     IPTSLVVAEEELPF"
                     /name="hypothetical protein"
     Typing          1541..2037
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.494"
                     /coverage="97.0"
                     /evalue="3.55e-179"
ORIGIN
        1 gtatctgttg tgcatggact gaaatgcact tattttatta tcttcaccga ccactccttt
       61 cagtggaagc ttgcaccacc catcctcatg cgggccgagt acgtctaccg tataaatttt
      121 tttacgttca gtctgcgaaa catttttgat taattttttt gcatatagaa ttttaagccc
      181 ttcgttaact aacgaacggc tcatagacgc agtctcacaa atttcgctaa aggttagctt
      241 gactgttcta caccccgaat cagactcttt gcaaaataag caaaaagtta aataaatttt
      301 taatgctgct atagcctttg ctgtatccag atcgctgaat tcagcgatct ggtttcctat
      361 ccataatgaa ggcatttttt tccagaacag cataggcaac ctctacgcaa tatttttaaa
      421 aatgaagtaa accaatccca atacggaaag cgataagaca agattggtta atacagtagc
      481 aaccttcgct aacctctcag ccagataaac gcttttttca aacattttca caccccttcc
      541 ctcgcccttg ttgtttttat tatctcaggc cgtgaaggcg ttgtaacgcc ttcacaaaaa
      601 tctatctaga tagataagga aagtgaaaaa tttcataggt gtttgtttat aaaataaatt
      661 tagttcattt atagtccaag tggaaactga attatggttc atggtgaacc ataatctaag
      721 tgcaccggca tattttctcc atgtatagat gcatccatac gatgaaaagc agccaatgca
      781 gttgtattta ccataacatc cgttgcgtgc accgcctgag aggccctcag ggcgtttctg
      841 ccgcgtccgg ccgatttccg gcacctttcc ctgtcccaaa cactgaaaaa cgcccctgat
      901 gatttttgcg cagctgcatg gctatgcaca gacatgcctg gccgccattt tagcggctaa
      961 aatccggcga gtgccctgaa agccatcatg aaccaccctg ataacggtga acgggtgcgc
     1021 agtttactgt gcggccgtga ggcgttggcc aggccgtcag gcatggcagg atttattgcc
     1081 aaaggggtcg gatttaacct ggggtgatca gcgcgcagag atgaacggga ttcggaaggg
     1141 gaacccttgc ggcgcggcga tgtatacatc gctaacgtgc ctgactcttt tcacagcaga
     1201 gggtagcggt agcaaacaag tagcaaacaa gtagcaaaaa gatagcaaac aagtagcaaa
     1261 cagatagcaa acaggtagca aaaagatagc aaacatacgg tgtcgggcgg aacctgacca
     1321 ggagtcattg gcgtgccggc ggtactccaa aaacacatct tgtcccgctg ttttgtgaat
     1381 aagccagcat ggctgtgggc acaaaacatt tcctgtccgc tgctttaacc ttgttaatcc
     1441 aggaacgtca ggtgacgtca ctttgcggcg ttagccctat cctgcatcgt attaaaaaat
     1501 gcagctatgg cgtgtacggt gccagaaata agtacgaaga tatgccgctt cctcgctcac
     1561 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggag ggaacaggca catcaaaaga
     1621 tgaagccatg agggacgatg agagggaaaa aaacgccgcg gcgttgccgg ttttccatag
     1681 gctccgcccc cctgacaagc atcacagaat ctgacgctca aatcagtggt ggcgaaaccc
     1741 gacaggacta taaagatccc aggcgtttcc ccctggtagc tccctcgtgc gctctcctgt
     1801 tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcggtttc tcattccacg
     1861 cctgacactc agttccgtga aggcagttcg ctccaagctg gactgtatgc acgaaccccc
     1921 cgttcagtcc gactgctgcg cctgttccgg taactttcga cttgagtcca acccggaaaa
     1981 gcacgacaaa acgccactgg cagcagccaa cggtaaaagg gtgtacaaga gattactaat
     2041 gcagagttct tgaagtatca gcctaactac ggctaaactg gaaggacgta tttggtggca
     2101 gcggtcttgt acaccctgtt accacggttc ataaacttgc cggtttaatg aaccttcgaa
     2161 aaaccacctt gccgggtggt tttttcgtat ctgggtcaag agactacgac gacaaacgat
     2221 gaatctcaag agaatatggt cactcccctg acaaaaatat catcaatcgc cacctcggca
     2281 ccgtgcaatc aaaacggaag ttcttcttca gcaacaacta gtgaggtagg tatcttccgg
     2341 taggttaaag ctctctcact acctgttttc atatagaaat aacaggagtc atgaaataaa
     2401 tctaatggct tatctacgat agcttttttt acaactctat caagtaaacc aatatgaatc
     2461 agatatgtaa tggctttatt taggtcagtt aatttgcact tcagctttgt acaaattgtt
     2521 ttttttctag ccaatgtgta gtcattacga ttatcgcgag cgtaaagaag atacatgtaa
     2581 gtttgtaagg ctagcaactc aaaagg
//
