LOCUS       NZ_CP104612             2817 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Klebsiella pneumoniae strain AHM8C161BI plasmid pHNAH8161B-8,
            complete sequence.
ACCESSION   NZ_CP104612
VERSION     NZ_CP104612.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30667241
            Assembly: GCF_030169265.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   He,W., Gao,M., Lv,L., Wang,J., Cai,Z., Bai,Y., Gao,X., Gao,G.,
            Pu,W., Jiao,Y., Wan,M., Song,Q., Chen,S. and Liu,J.H.
  TITLE     Persistence and molecular epidemiology of blaNDM-positive
            Gram-negative bacteria in three broiler farms: A longitudinal study
            (2015-2021)
  JOURNAL   J Hazard Mater 446, 130725 (2023)
   PUBMED   36630875
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   He,W., Gao,M., Lv,L. and Liu,J.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2022) College of Veterinary Medicine, South China
            Agricultural University, Wushan, Tianhe District, Guangzhou,
            Guangdong 510642, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_030169265.1-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 05:51:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,218
            CDSs (total)                      :: 6,096
            Genes (coding)                    :: 5,888
            CDSs (with protein)               :: 5,888
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 208
            CDSs (without protein)            :: 208
            Pseudo Genes (ambiguous residues) :: 0 of 208
            Pseudo Genes (frameshifted)       :: 72 of 208
            Pseudo Genes (incomplete)         :: 138 of 208
            Pseudo Genes (internal stop)      :: 38 of 208
            Pseudo Genes (multiple problems)  :: 32 of 208
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104612.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="AHM8C161BI"
                     /isolation_source="chicken feces"
                     /db_xref="taxon:573"
                     /plasmid="pHNAH8161B-8"
                     /geo_loc_name="China"
                     /collection_date="2018-11"
     gene            67..309
                     /locus_tag="N5862_RS30950"
                     /old_locus_tag="N5862_30935"
     CDS             67..309
                     /locus_tag="N5862_RS30950"
                     /old_locus_tag="N5862_30935"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(655..1077)
                     /locus_tag="N5862_RS30955"
                     /old_locus_tag="N5862_30940"
     CDS             complement(655..1077)
                     /locus_tag="N5862_RS30955"
                     /old_locus_tag="N5862_30940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_022644822.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKDIKVID
                     VSELIRVFGSLDGVTDTVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPSHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1077..1268)
                     /locus_tag="N5862_RS30960"
                     /old_locus_tag="N5862_30945"
     CDS             complement(1077..1268)
                     /locus_tag="N5862_RS30960"
                     /old_locus_tag="N5862_30945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004207910.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDSLQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1684..1827
                     /locus_tag="N5862_RS31160"
     CDS             1684..1827
                     /locus_tag="N5862_RS31160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058671098.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHDLSPLAAATGNGFAEQRSV
                     EV"
                     /name="hypothetical protein"
     gene            2246..2671
                     /locus_tag="N5862_RS30965"
                     /old_locus_tag="N5862_30950"
     CDS             2246..2671
                     /locus_tag="N5862_RS30965"
                     /old_locus_tag="N5862_30950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676030.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="WP_015328245.1"
                     /translation="MGSYVDQSLTRNESVISRAQTSWIPTIIPVIIGILLLPFYGLGLL
                     IIIPVLLRVWSTELALTNQRVIAKVGLIRRNTVELRIDKVESLGIHQGILGRIFNYGSI
                     VIKGTGGTNTPIPNIKAPMQFRSIVNNHIEEMNSKQQ"
                     /name="PH domain-containing protein"
     Typing          1427..1792
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.095"
                     /coverage="85.0"
                     /evalue="1.36e-162"
ORIGIN
        1 accacccgaa aacccgccag aaacgcgcag gctctgtttt tacggcatgc atgagtatgc
       61 gcgcaagtgc atagcgcgga aattcacacc attcagccag gggaagtcct gtttccggcg
      121 ttcttctcct ccgaaaacgg tgaacgggga cgcaatttat tgcgtggccg tgaagcgttg
      181 ccggcgcagc cggaaaaccg gttttcagca ccttcaggaa tcgggttcgt cgacggccgg
      241 caatcacgtt atggcaagtc gccggacgac tcaccacacg attcaccggc gattcgaatc
      301 gtgaattgaa tcaagagtca ccatgagcaa aaaacaccca aagtgatgaa ttgaatcaag
      361 agtcgattca tcataacaat atgataataa agcgtgattc acatatgaat cgtgggcaat
      421 tcgtcagcga ttcgccatag agtcgtccac gacttggatc tgcactttgt aacacatcac
      481 cgcacattta tttttcatta acccattgtt gtaaataagg aatctgcaaa attttaattt
      541 tgcgtggggt gtgggtattt ttagtggtga ggagcgttca gagatctggc gatttttggg
      601 aaggttcaga aactctccgg gatggccgga gaggaaaact caagcatggc aggatcagga
      661 ttttttccag aatttccacc acggggaatg ggatggtgaa gaaggttcgt gcttgtgctc
      721 cagaagctgc atagcctgac gaagggaatc aatatgctca tctcgagcct taacagcccc
      781 tttaagacca tcgttttcag cttcaagtaa agcgattcta tgttgtaaat catgtaaacc
      841 aacgctgtta atacctgtag attctgcgtc actttttaca tcatatttta cagtatcagt
      901 tacaccatca agagaaccaa acaccctaat aagctcggat acatcaataa ctttaatatc
      961 cttcccgtgc tttacaacag aggtataact taacttcccg ctgttaatca tcttgtaaat
     1021 agtgggacga ctaaccttaa caagccgtgc agcttcacta atagacactc tggccatcag
     1081 ttaccatcct gtaatgaatc cagtctcgag gagagtgtac ggaaaagatg ctctgcatca
     1141 tcgtggagtt tctcacaaag gtcggcctca gcatcaagat caagctcgtt aagcttctcc
     1201 agcaggagca gagactgatt ctggatgaat ttagccatgt tcagcgccgt tttctgttgt
     1261 ttcgtcataa agggcctcag agcgtcagtg ctcagtgtaa aacctgtctc ttttccgctt
     1321 cctcgctcac tcagtcgcta cgctccggtc gttcggctgc ggcgagcggc aggagcatgc
     1381 atagaaaagc tcgagaaaac cgagcggtca aaggaggaaa aaaaggaaag gccacggcgt
     1441 tgccgttttt ccataggctc cgcccccctg acaagcatcg cagaaacgga cgctcaaatc
     1501 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
     1561 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cactccgctg ttatggccgc
     1621 gtttatctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     1681 tgtatgcacg aaccccccgt tcagtccgac tgctgcgcct tatccggtaa ctatcgtctt
     1741 gagtccaatc cggaaagaca cgacttatcg ccactggcag cagccactgg taacggattc
     1801 gcagaacaaa gaagtgttga agtgtgaggt gaagtacagc tacactgtaa taactgattt
     1861 ggtttctgtg ctctgcgaaa gccagttacc tcggttcaaa gagttgctgc tctaagaacc
     1921 ttcgaaaaaa ccgccttgct gggcggtttt ttcgtttcca gagcaagaga ttacgacgca
     1981 acggacagga tctcaagaag atcatctgtg atattcgaca gatatgggat aaaggattgc
     2041 aacgccaaga gtatatgaga catagcagca gccaccacca ccacagaatc aagccagttg
     2101 cggcacgaaa ggaaagaacc gaactgtgcg atggtcgcta cgctctccgt aaattttgca
     2161 tacccaccac ggagacataa agtttctatt gctgattcgc cttaaaaagg gaaaaatcct
     2221 attttttcta aatggatata accttatggg aagctacgta gaccaatcat taacgagaaa
     2281 tgaatccgta atatctcgcg ctcaaacctc ctggattcca acaatcatcc cagtaattat
     2341 cggaatcttg ttgctgcctt tttacggctt agggttactt ataataatcc ctgtgctcct
     2401 tagagtttgg tcaacggagc ttgcattaac taaccaaaga gtcatcgcaa aagtgggttt
     2461 gatccgacgc aatacggtgg aactccgtat tgataaagtt gaaagccttg gcatacatca
     2521 agggatactg ggacgaatct ttaactatgg gtctatcgtg attaaaggca ctggcggaac
     2581 caacactcca attccaaaca tcaaggcgcc aatgcaattc agatcgattg tgaacaatca
     2641 tatcgaggaa atgaattcta agcagcagta gcttcctcgt agcattacaa aaaaaagccc
     2701 tacagggctt ttttttgtac ctgccaatca gctggattta ccataacacc cgttatcagc
     2761 accgcctgag aggccctcag ggcgtttctg acgggaaacc ggtaaaacag gcaacaa
//
