LOCUS       NZ_CP104597             6647 bp    DNA     circular CON 01-MAR-2024
DEFINITION  Escherichia coli strain EC9952 plasmid pEC9952-2, complete sequence.
ACCESSION   NZ_CP104597
VERSION     NZ_CP104597.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30844958
            Assembly: GCF_028421485.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Lin,J., Zhang,C., Wen,F. and Li,X.
  TITLE     Characterization and Genomic Analysis of Escherichia coli EC9952
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Lin,J., Zhang,C., Wen,F. and Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-SEP-2022) Department of pediatrics, Zhuhai People's
            Hospital, 79 Kangning Road, Zhuhai 519000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Hifiasm v. 0.13-r308; Canu v. 2.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 58.67x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/01/2024 04:02:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,958
            CDSs (total)                      :: 4,839
            Genes (coding)                    :: 4,639
            CDSs (with protein)               :: 4,639
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 83 of 200
            Pseudo Genes (incomplete)         :: 118 of 200
            Pseudo Genes (internal stop)      :: 43 of 200
            Pseudo Genes (multiple problems)  :: 43 of 200
            Pseudo Genes (short protein)      :: 3 of 200
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104597.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC9952"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC9952-2"
                     /geo_loc_name="China:Zhuhai"
                     /lat_lon="22.28 N 113.58 E"
                     /collection_date="2022-06"
                     /collected_by="Zhuhai People's Hospital"
     gene            join(5250..6647,1..171)
                     /gene="cea"
                     /locus_tag="N5B57_RS24705"
                     /old_locus_tag="N5B57_24645"
     CDS             join(5250..6647,1..171)
                     /gene="cea"
                     /locus_tag="N5B57_RS24705"
                     /old_locus_tag="N5B57_24645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            557..694
                     /locus_tag="N5B57_RS24710"
                     /old_locus_tag="N5B57_24650"
     CDS             557..694
                     /locus_tag="N5B57_RS24710"
                     /old_locus_tag="N5B57_24650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(893..1051)
                     /locus_tag="N5B57_RS24825"
     CDS             complement(893..1051)
                     /locus_tag="N5B57_RS24825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(1721..1912)
                     /locus_tag="N5B57_RS24715"
                     /old_locus_tag="N5B57_24655"
     CDS             complement(1721..1912)
                     /locus_tag="N5B57_RS24715"
                     /old_locus_tag="N5B57_24655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1976..2299
                     /locus_tag="N5B57_RS24720"
                     /old_locus_tag="N5B57_24660"
     CDS             1976..2299
                     /locus_tag="N5B57_RS24720"
                     /old_locus_tag="N5B57_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2508..>2939
                     /locus_tag="N5B57_RS24725"
                     /old_locus_tag="N5B57_24665"
                     /pseudo=""
     CDS             2508..>2939
                     /locus_tag="N5B57_RS24725"
                     /old_locus_tag="N5B57_24665"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2977..3495
                     /locus_tag="N5B57_RS24730"
                     /old_locus_tag="N5B57_24670"
     CDS             2977..3495
                     /locus_tag="N5B57_RS24730"
                     /old_locus_tag="N5B57_24670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            3502..3735
                     /locus_tag="N5B57_RS24735"
                     /old_locus_tag="N5B57_24675"
     CDS             3502..3735
                     /locus_tag="N5B57_RS24735"
                     /old_locus_tag="N5B57_24675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4053..4472)
                     /locus_tag="N5B57_RS24740"
                     /old_locus_tag="N5B57_24680"
     CDS             complement(4053..4472)
                     /locus_tag="N5B57_RS24740"
                     /old_locus_tag="N5B57_24680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(4553..4990)
                     /locus_tag="N5B57_RS24745"
                     /old_locus_tag="N5B57_24685"
     CDS             complement(4553..4990)
                     /locus_tag="N5B57_RS24745"
                     /old_locus_tag="N5B57_24685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     Typing          1549..1636
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(980..1535)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.52"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2290..3839
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttagagaaga aagctgcaga tgcaggggtg agttatgttg ttgctttact ttttagcttg
       61 cttgctggaa ctacattagg tatttggggt attgctattg ttacaggaat tctatgctcc
      121 tatattgata agaataaact taatactata aatgaggtgt tagggattta attattcttt
      181 attgatataa aaagtcctag caatccaaat gggattgcta ggaccaaaca aagtagatta
      241 tatagcataa ataggtttaa ttttgctacg ggggcgttat ttaggttttt tcttctttcg
      301 aaaaaatctt tctttatgaa gttaaaagct atgtgttcaa tagcatattt tgaatatgga
      361 catagaatag tgcttatcac tattgcatat agcatcttat ctgacacaag gaaataatac
      421 ccctcgctgt tttttgttat aaggtatata tatataagtg tgcagtacag gccaaataaa
      481 atatttttta tgtagtatct taagctcata aattaaacct cgccatatat tcttttcatt
      541 ttataaggat cgagttatga ggaaaagatt ttttgtggga atattcgcga taaacctcct
      601 tgttggatgt caggctaact atatacgtga tgttcaggga gggaccatcg caccatcctc
      661 ctcttctaaa ctgacgggga tcgcggttca gtagaaaaga tcaaaggatc ttcttgagat
      721 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accaacggtg
      781 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      841 gcgcagatac caaatactgt ccttctagtg tagccgtagt cgggccacta cttcaagaac
      901 tctgtagcac cgtttgtgcc atcatcgctc tgctaatccg gttaccagtg gctgctgcca
      961 gtggcgttaa ggcgtgcctt accgggttgg actcaagacg atagttaccg gataaggcgc
     1021 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     1081 ccgaactgag ataccaacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     1141 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     1201 cagggggaaa cgcctggtgt ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     1261 gtctattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc tgctacgcgg
     1321 ccttcttcct gtttctggtc ttttgctcac atgttctttc cggccttatc ccctgattct
     1381 gtggataact gtgttaccgt ttttgtgtga gtcagtaccg ctcgccgcag tcgaacgacc
     1441 gagcgtagcg agtcagtgag cgaggaagcg gaaaagcgcc tggacgtgca ttttctcctt
     1501 acgcatctgt gcggcatttc acacccggca tggcgtactt ttcatacaat ccgcactgat
     1561 gccgcatggt taagccagta tacactccgc tatcgctacg tgactgggtc agggctgcgc
     1621 cccgacaccc gctaaaacct gctgacgcgc cctgacgggc ttgtcagctc ccggcatccg
     1681 ctcacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     1741 ccccgaaacg tgcgaggcag ctgcggtaaa gctcatcggc gtggtcgtga agcgattcac
     1801 aaatatcggc ctgttcatct gcgtccagtt cgttgagctt ctccagcagc gttaatgtct
     1861 ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttagtc actgatgcct
     1921 ccgtgtaagg gggatttctg ttcatggggt aatgataccg atgaaacgcg agaggatgct
     1981 cacaatacgg gttactgatg atgaacatgc ccggttactt gaacgctgtg agggtaaaca
     2041 actggcggta tggatgcggc gggtctgcct gggggagccg gttgcccgtt ccggaaaact
     2101 gccgacactg gcaccgccgt tactgcgtca gctggccgcc atcggaaata acctgaatca
     2161 gacagcccgt aaggtgaaca gcgggcagtg gtcttccggt gaccgggttc aggtggtggc
     2221 cgcactgatg gccatcgggg atgagctgcg ccggctgcgt ctggccgtca gggaacaggg
     2281 ggcgcgggat gatagttaaa tttcatgcca ggggaaaagg tggtggcagt ggtccggttg
     2341 attacctgct ggggagggag cgtaaccgcg aaggcgcaac ggtgcttcag ggtaatccgg
     2401 aagaagtccg ggaactcatc gatgccacgc catttgcgaa gaaatacacg tccggtgttc
     2461 tgtcgttcgc ggagaaggag ctgccgccgg gaggacgtga aaaagtgatg gcgagctttg
     2521 agcgtgtact gatgcccggt ctcgaaaaaa atcagtacag catcctgtgg gtggagcacc
     2581 aggacaaggg acggcttgag ctgaattttg tcattccgaa catggagcta cagaccggaa
     2641 aacgcctcca gccgtactac gaccgcgcag acaggcctag aattgatgcc tggcagacgc
     2701 tggtgaatca ccattacggg ctgcatgacc cgaacgcccc ggagaaccgc aggacgctga
     2761 cactccctga taacctgcct gaaacgaaac aggcgcttgc tgagggcgtc acgcgaggta
     2821 tagatgcact ttaccatgcc ggagagataa aaggccgtca ggatgtgatt caggcgctca
     2881 ctgaggcggg gctggaagtg gtcagggtga cgcgaagcag tatcagcatt gcagatccga
     2941 acggcgggaa gaatatcagg ctgaaaggag cattttatga gcaatctttt gcagacgggc
     3001 gcggagttcg agaaaaagct gaaagagaga gccgaatcta cagagaaaat gctgaacaac
     3061 gagttcagga ggctcggcga atctgtaagc gaggctgtga catcaaacga gacgaaaatc
     3121 agagacgcta tagccctgtt cacagcctcg acagaggaat cgctggaaaa acaccgggaa
     3181 ggggtgaaag aggcgatgat gcagcacagg agggacgtgt taaagctggc agggaatacg
     3241 ggcatgatgt tactggggat agtctttctc ctgtttaccg cgagtggcgg gacgctctgg
     3301 tatcttggag ggaggataca ggcgaacctg gaagaaatca ggaagcagga agagacattg
     3361 cagaaactga acgcgaagac atggggcgtg gagtttgtgc aggacgggaa caggaaattc
     3421 cttgtccttc cgtacgggaa atcagcggag gtgattccct ttcaggggaa agagtgggta
     3481 catctgaagg agtgacacag agtgacagag ctggaaacac atttgctgaa cgccttagag
     3541 cagctgcaac aggactatat gcagcggctg agcgaatggg agagcgcctt cgtggaattg
     3601 cagaagatgt tttcgcttac gcaacgggac aacgcgatgc tgaacgagcg ggtcatgcag
     3661 ttgagtcagc aggtgcagca cttgagcgag cagacagaac gcttgagcca gttatacagc
     3721 gagaactgga gataagagag gaacggctga tacaggagcg cgaacatgtg ttatccctgg
     3781 aacgggagcg tcagccggaa atacaggaac gcacgctgga tggcccttcg ctgggatggt
     3841 gaaaccatga aaaatggcag cttcagtgga ttaagtgggg gtaatgtggc ctgtaccctc
     3901 tggttgcata ggtattcata cggttaaaat ttatcaggcg cgatcgcggc agtttttcgg
     3961 gtggtttgtt gccattttta cctgtctgct gccgtgatcg cgctgaacgc gttttagcgg
     4021 tgcgtacaat taagggatta tggtaaatcc acttactgtc tgccctcgta gccatcgaga
     4081 taaaccgcag aaatcgtgtc agccagcagc ctggattttt tcggggtaag gtctttgctg
     4141 cggtaatcag tacccgcaaa ttgagttgtg ccccggagtg agtttaaaaa ttcctggctg
     4201 tgaaactgct gaatgcgttg ttgcacatca ctccgtgatt ttcctgtcgc tttatcttta
     4261 gctcccatat cccagaactg ccgaaaaaaa ggcaccatca ttcgtgttgt gtcggctttt
     4321 tgggtggaaa agttgggatc aaaaccgtca tcagaagcat atacgtaatg ccgggtatgt
     4381 tgttccacgc tgccctttgg ggtacaggct gtgagagcta ctgttgctgt tatcatggcg
     4441 ataagacaaa gtttgtttcc ggtacgtctc atgagtgtct ccctacctta aagtatttat
     4501 tctcggtgag ttattatccg aagactattt tatcatcagt ctctgacagc gttcagcgac
     4561 cgaacaacct ggaccagaat ccccgtttct tttccagttc cagtgcctgc ctgagctggg
     4621 ctatctcatt ttgtagctgt tcccgttctg cttcctggcg tctgcgatcc atatcctgtg
     4681 cctgtttatc ctcaagcatc agcgtcaggc attgtttcag ctcattcagc tcccggagaa
     4741 tgcgttctgt ctgctgatca tgtggatttt ctgcatgtcc ctcactgtgc ctttctggtg
     4801 tctcattctg ctttaattcg ccgtatgccc ggatcagttc actggtttca aactcccgtc
     4861 gtccgtccct gccggtacgg taactgacaa ggcctgcgcg catatcacgg taaaactgtg
     4921 aacgcgatct gcctgtcatt tttagtgcgt cccggagtgt gtgccatgcc ataaagtgac
     4981 agtgtcccat agatgtctca tctcatagtt tcagtaaaac ataatgaggt ctgagaacgg
     5041 taatgtttgt gctggttttt gtggcatcgg gcgagaatag cgcgtggtgt gaaagactgt
     5101 ttttttgatc gttttcacaa aaatggaagt ccacagtctt gacagggaaa atgcagcggc
     5161 gtagctttta tgctgtatat aaaaccagtg gttatatgta cagtatttat ttttaactta
     5221 ttgttttaaa agtcaaagag gattttataa tggaaaccgc ggtagcgtac tataaagatg
     5281 gtgttcctta tgatgataag ggacaggtaa ttattactct tttgaatggt actcctgacg
     5341 ggagtggctc tggcggcgga ggtggaaaag gaggcagtaa aagtgaaagt tctgcagcta
     5401 ttcatgcaac tgctaaatgg tctactgctc aattaaagaa aacacaggca gagcaggctg
     5461 cccgggcaaa agctgcagcg gaagcacagg cgaaagcaaa ggcaaacagg gatgcgctga
     5521 ctcagcgcct gaaggatatc gtgaatgagg ctcttcgtca caatgcctca cgtacgcctt
     5581 cagcaacaga gcttgctcat gctaataatg cagctatgca ggcggaagca gagcgtttgc
     5641 gccttgcgaa agcagaagaa aaagcccgta aagaagcgga agcagcagaa aaggcttttc
     5701 aggaagcaga acaacgacgt aaagagattg aacgggagaa ggctgaaaca gaacgccagt
     5761 tgaaactggc tgaagctgaa gagaaacgac tggctgcatt gagtgaagaa gctaaagctg
     5821 ttgagatcgc ccaaaaaaaa ctttctgctg cacaatctga agtggtgaaa atggatggag
     5881 agattaagac tctcaattct cgtttaagct ccagtatcca tgcccgtgat gcagaaatga
     5941 aaacgctcgc tggaaaacga aatgaactgg ctcaggcatc cgctaaatat aaagaactgg
     6001 atgagctggt caaaaaacta tcaccaagag ccaatgatcc gcttcagaac cgtccttttt
     6061 ttgaagcaac cagacgacgg gttggggccg gtaagattag agaagaaaaa caaaaacagg
     6121 taacagcatc agaaacacgt attaaccgga taaatgctga tataactcag atccagaagg
     6181 ctatttctca ggtcagtaat aatcgtaatg ccggtatcgc tcgtgttcat gaagctgaag
     6241 aaaatttgaa aaaagcacag aataatctcc ttaattcaca gattaaggat gctgttgatg
     6301 caacagttag cttttatcaa acgctgactg aaaaatatgg tgaaaaatat tcgaaaatgg
     6361 cacaggaact tgctgataag tctaaaggta agaaaatcgg caatgtgaat gaagctctcg
     6421 ctgcttttga aaaatacaag gatgttttaa ataagaaatt cagcaaagcc gatcgtgatg
     6481 ctatttttaa tgcgttggca tcggtgaagt atgatgactg ggctaaacat ttagatcagt
     6541 ttgccaagta cttgaagatt acggggcatg tttcttttgg atatgatgtg gtatctgata
     6601 tcctaaaaat taaggataca ggtgactgga agccactatt tcttaca
//
