LOCUS       NZ_CP104552             5596 bp    DNA     circular CON 09-OCT-2023
DEFINITION  Klebsiella pneumoniae strain K28074 plasmid punamed2, complete
            sequence.
ACCESSION   NZ_CP104552
VERSION     NZ_CP104552.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30825845
            Assembly: GCF_025397975.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Pu,D.
  TITLE     A 1.5-Year Within-Patient Resistance evolution of a fatal ST11
            carbapenem-resistant hypervirulent Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Pu,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2022) China-Japan Friendship Hospital, Peking
            Union Medical College, No.2 Yinghua dong Avenue, Beijing 100020,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. v3.15.5; Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 196.5x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/09/2023 03:06:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,360
            CDSs (total)                      :: 5,239
            Genes (coding)                    :: 5,126
            CDSs (with protein)               :: 5,126
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 34 of 113
            Pseudo Genes (incomplete)         :: 76 of 113
            Pseudo Genes (internal stop)      :: 12 of 113
            Pseudo Genes (multiple problems)  :: 9 of 113
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104552.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="K28074"
                     /isolation_source="bronchoalveolar lavage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="punamed2"
                     /geo_loc_name="China:Beijing"
                     /collection_date="2020-01-04"
     gene            104..613
                     /locus_tag="N5911_RS26695"
                     /old_locus_tag="N5911_26695"
     CDS             104..613
                     /locus_tag="N5911_RS26695"
                     /old_locus_tag="N5911_26695"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            620..832
                     /locus_tag="N5911_RS26700"
                     /old_locus_tag="N5911_26700"
     CDS             620..832
                     /locus_tag="N5911_RS26700"
                     /old_locus_tag="N5911_26700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1499..1723
                     /locus_tag="N5911_RS26705"
                     /old_locus_tag="N5911_26705"
     CDS             1499..1723
                     /locus_tag="N5911_RS26705"
                     /old_locus_tag="N5911_26705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            1707..1973
                     /locus_tag="N5911_RS26710"
                     /old_locus_tag="N5911_26710"
     CDS             1707..1973
                     /locus_tag="N5911_RS26710"
                     /old_locus_tag="N5911_26710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2043..2393)
                     /locus_tag="N5911_RS26715"
                     /old_locus_tag="N5911_26715"
     CDS             complement(2043..2393)
                     /locus_tag="N5911_RS26715"
                     /old_locus_tag="N5911_26715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2459..3004)
                     /locus_tag="N5911_RS26720"
                     /old_locus_tag="N5911_26720"
     CDS             complement(2459..3004)
                     /locus_tag="N5911_RS26720"
                     /old_locus_tag="N5911_26720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(3030..3356)
                     /locus_tag="N5911_RS26725"
                     /old_locus_tag="N5911_26725"
     CDS             complement(3030..3356)
                     /locus_tag="N5911_RS26725"
                     /old_locus_tag="N5911_26725"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(3714..3872)
                     /locus_tag="N5911_RS26830"
     CDS             complement(3714..3872)
                     /locus_tag="N5911_RS26830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            4512..4688
                     /locus_tag="N5911_RS26730"
                     /old_locus_tag="N5911_26730"
     CDS             4512..4688
                     /locus_tag="N5911_RS26730"
                     /old_locus_tag="N5911_26730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4678..5022
                     /locus_tag="N5911_RS26735"
                     /old_locus_tag="N5911_26735"
     CDS             4678..5022
                     /locus_tag="N5911_RS26735"
                     /old_locus_tag="N5911_26735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(5204..5449)
                     /locus_tag="N5911_RS26740"
                     /old_locus_tag="N5911_26740"
     CDS             complement(5204..5449)
                     /locus_tag="N5911_RS26740"
                     /old_locus_tag="N5911_26740"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032433971.1"
                     /translation="MQPQSPVHHGLPRIDARTVCPVVVRLETPAGQQFRVRYQKIQFQP
                     PLVPVFNPQHAVLVFIESGHQNPLEARHQLFTLAGR"
                     /name="hypothetical protein"
     Typing          4347..4426
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(3764..4161)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          2..420
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="86.0"
                     /evalue="3.09e-83"
     Typing          5013..5596
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.36e-123"
ORIGIN
        1 gcgctggaaa gcgcaggttt tgaggtcgtg cgcaccacaa agagcagcat cagcattgcc
       61 gacccggacg gagggcgaaa catccgactt aagggagcca tctatgaaca gtcttttaac
      121 gctggcgaag gacttagagc agaaatcgaa agcgcagcag cagagtaccg gcgaaatgct
      181 gaaagccgca ttcagcgagc acgagaagtc tgtcagagcg gaactgagcg aaagcgggaa
      241 gagaatcagc gccgccatcc gcgcccacga gcagggtatg agcgcggcca tgcagtcgaa
      301 ccgcctgagc gtgatgcgta tggtcgggcg gacatggctg accatcacga tggtgtcagg
      361 actgctgttc gccagcctga gcggggtgtt gtggtatcag gggagcttga tagcgtcaaa
      421 tctggccgaa atcgaccggc agaacgcagc gctgtcgaag ctgaacgcga agacctgggg
      481 cgtgacgtac ctggaagaca gcaacggacg ttttctggtg ctgccgaagg ggacggccgt
      541 agacaggacg cagagctgga cggtcgggaa cgggcggtcg aagcagaacg cgctgagact
      601 ggtgaaggag taagtgccga tgaccgagct ggaaaaacag ttgctgagcg cattagagca
      661 gctacagcag gactactcga aaaggctgga cgagtgggag agcgccttcg gggaatggcg
      721 gaaaatgtca gggcttatgc aacgggagaa cgcggcgctg agcgagcgcg tgacgcgctt
      781 gagtcagcag gtggaacgct tgagcgggca actgcagcgt ttgagccggt agtgcagcgt
      841 catgagatgg ccgtggccgc agaacgggcg cacgagcaac ggcagcacga aaaggaactg
      901 acggaagcgc gttcccggca gcgaagctat gacggtccgt cgctgggctg aaatgcagga
      961 aaacagggtt ctcatcgggc atttacgctg aaaccggatg tcattcacct ggctgcatgg
     1021 ctatgcagcc aggtaaaatt tatctgtcgc gaacggggcg gtttatcggg tggtttgttg
     1081 ccggttttac ctgactgcca ccccgttcgc ggcgaacccg tccggggcgg tgcgggcaac
     1141 gggtgttatg tttaatcagc gtgaacggca gatacaaaaa agccccggag ggcttttaac
     1201 tactgactta tttttttgaa gatttgctgg gtttcaattt gtcagtattt cccggcgttt
     1261 ttttattgtc acgctggcgt ttatcaggtt cccctgttga atcggcaatt cgtttcggac
     1321 ccggcttaag acccataatc gttatccttt caatagttaa gaggaaagcc ctcaagctag
     1381 atatattgac cgcgttacgg attatcaaga ggggctaaca agattatttt ttgttctttt
     1441 aggttgattg tggtactgtt gatatacatg tatagcaaat ggtgttcggg aggtttatat
     1501 gcttgctatc cggttatctg atgagattga gtcccgtctg gactcgctgg cgaagcaaac
     1561 cggcagaaca aagacgtttt atgcgcggga agcaatactg gcgcatctgg aagacctgga
     1621 ggattattat ctttcagcag aaactgctgc acgcgttcgc cgtggtgatg aagcagtgca
     1681 ttcgtctgaa gacgtgagga agtcacttgg tctggacgat taactattcc gatcgggcgc
     1741 tcaaatcgtt acgcaagatg gacaaacaga acgcacgacg gattgtggat tttatgagtt
     1801 tacgcattgc agttgctgcc gatcctcgcc agtcagggaa gccgctcaaa ggtgagctgg
     1861 gcgagttctg gcgctatcgg gtgggagatt atcgcgttct gtgtgagatc cgagatgacg
     1921 agcttgttat ccttgccgcc acgattggac atcgccgcga agtttacgac tgaagcccgc
     1981 tatgcgggct tttttgatgc cgacaacagg tattatggta aatactcgac taactacgtg
     2041 ttctactgtt tgtcggtttt agttaaccag caggttgtct taacgataat gtagccgtta
     2101 tttttaacta ataaggtcat attgttaacg tcaggcataa taaaagacat tatggcctca
     2161 gagttatcat cagaagcagc tctctgcatt actacttcga ttcccttttc tttgaatcta
     2221 taagtatcca gttgcctatt aaacttagaa agacctacaa ccatacttag tttgttatct
     2281 ttaacagtaa gagatgcttc ctgagtacca ttacatcggt agtaagcggt gtaaccgtca
     2341 gcaaaagatc gaccggggaa tataaatgct aggaacgcaa ttaaccaata catagttgag
     2401 aatcttggta ttacacattt cactatcctc tctttcattt ttcgttcacc atactttgtt
     2461 atctgatttt taatttttct attttataaa ccaagtctct accaaaaagg cgaaaattat
     2521 cctcaaataa taataattgt ttaagaacat ccatgaaagc agctgcttca ccctcagtta
     2581 tgggagctac tctatataac atataacctg agttaagggt ttcttttata ctttcttcaa
     2641 tgctcattat aaatattagt ggattatcat ttttcttccc ccatgggttt ctaattaatc
     2701 cttcgatgta ctcaggtcct ttgcgtccta ttgcctgcgc aatatttttt atttcatctg
     2761 cgtgagggct ttgttgtaca gctttgttta tgcctgcttg agtgattttc agagactcta
     2821 caccgtgtat gttgtatgag ataaaatgta ataaattaag atgcaatgca attaatccat
     2881 ttttgtttac atctgagtat ttggataaaa aacgtaatga agtgttaact ctcccttgaa
     2941 gcctaacaat tgggagtgct gccttgtata tttccttctc cgttaaatac cgtttaaaaa
     3001 acataattct ccgcctccgt atagcgtgcc tattttattt cgtcgtaaaa cttagctctg
     3061 aaatattcct ttagaaccat catgtcatta ataattgaat ctatctcatc cattttttct
     3121 tggtattctt ttaatggtga agggagcggt aactcaatat ttgaaaagtg attggcgaga
     3181 tagggatcaa ggtttctcat ccattcaggc ttttcgccaa tgcgttcttc aactgaaagt
     3241 gaacctgata atctctggtt aataagatca cttagactca ttttggtttt attcgaaagc
     3301 cgttgtaagg attgctttaa tcctatatct agcatgatat ttgttctttc tctcattacc
     3361 cccccttgct attgcgtttt ttgttgcgca caaagctacc attgcgcatt aagaaacgca
     3421 ataaaaaagg agggagggag tggtttgtac atgatgtgca gtgcgctgag ggcatcagtg
     3481 gcgggtgttt ttccgtggga tggatggagc tccggggata acagaactga cgatcattta
     3541 aaggatcttc ttgagatcct ttttttctgc gcgtaatctc ttgcactgta aacgaaaaaa
     3601 ccgcctgggg aggcggtttg atcgaaggtt aagtcagttg gggaactgct taaccgggta
     3661 actgggtata cgagaccgca gataccaaaa ctgttctttc agtgtagccg cagttaggca
     3721 cccacttcaa gacctctcaa tatctctctc gtatatatcc agttaccaat ggctgctgcc
     3781 agtggcgttt ttgcgtgtcc gtccgggttg gactcaagat gatagttacc ggaaagggcg
     3841 cagcagtcgg gctgaacggg gggttcgtgc atacagccca gcttggagcg aacgacctaa
     3901 accgagccga gataccagca gcgtgagcta tgagaaagcg ccacgcttcc cgaaagggaa
     3961 aaaggcggaa caggtatccg gtaaacggca gggtcggaac aggagagcgc acgagggagc
     4021 cgccggagag aaatcaccgg catctttaag tcctgtcggg tttcgtcctc ctctgacttg
     4081 agcgtccatt tctgtgatgc tcgtcagggg aggcggagcc tatggaaaaa cggcaacgcc
     4141 gcatcctttt ctccggccct ttctgatttg agcgcctttg ctttatcccg ttatccgtga
     4201 gtcggtcacg cccgccgcag ccgaacggca ggagcgcagc taccgagtga gcgaggaggc
     4261 gtcatttcat ctgtccgtgc atggtattgc ggacgctcct gagccacata tcacacctgc
     4321 cacatgaggc actttcatga atcgtgctct catgccaaca tagtaagcca gtatacacag
     4381 cgtaaacgct gtgactggtt cagggctgcg ccccgaaacc cgctaacacc actgacgcgc
     4441 tggcgcttgt ccaacgccgg agcgacccgg aaaacttttc cgtgccgtcc ggcgtttgcg
     4501 aaggagggat aatgcagaag ccgaagaaga agccgctcgt cgtaaaaaaa agatcctggc
     4561 atccggcagt gatggcaaat ctgatgcgtc aggcacagga ggaagattac cgccgggagc
     4621 agatggcaga gcttgagcgt tcagcccggt cacattttgc ggatatcgga ggagaggatg
     4681 gcggttaagc gtacccgttt tttaggtatc cgggtgaccg atggggagta ccagcagctg
     4741 ctggagcgct gcaatggaag gcaactggcg gtgtggatgc gggaaacctg tctcgatacg
     4801 cgcccggcgc ggtcgttgcg gctgccgtcc atcgatccgg ttttgctgcg ccagcttgcc
     4861 gggatgggga acaacctcaa ccagatcgcc cggaaaatta acggcggcca gtggtcaggc
     4921 gctgaccggg ttcaggtggt ggccgcgctg atggccattg atgccgaact cgagcggctg
     4981 cggcatgccg tgctggaaaa gggggcagac gatgatcgtt aagtttcatc cgcgagggcg
     5041 aggcggcggt gccgggccgg tggattatct gctggggaaa gaccgccagc gcgacggggc
     5101 catcgtcctg cagggaaagc cggaggaggt ccgggagctt atcgacgcct cgccctacgc
     5161 caaaaagtac acctccggcg tcctgtcttt tgctgaacag gatttaccgc ccggccagcg
     5221 tgaaaagctg atggcgagct tcgagcgggt tctgatgccc ggactcgata aagaccagta
     5281 cagcgtgctg tgggttgaac accgggacaa ggggcggctg gaactgaatt ttctggtacc
     5341 gaacacggaa ctgctgaccg gcaggcgtct ccagccgtac tacgaccggg cagaccgtcc
     5401 gcgcatcgat gcgtggcaga ccgtggtgaa cgggcgactg gggctgcacg acccgaacgc
     5461 accggagaac cggcgcgcgc tggtgacgcc atcttcgtta cccaaaacga agcaggaagc
     5521 cgcagaggct attacgcggg gcttactggc cctcgcctcg tcaggggagc ttaaaacgcg
     5581 tcaggacgtc actgag
//
