LOCUS       NZ_CP104543             6647 bp    DNA     circular CON 07-OCT-2023
DEFINITION  Escherichia coli strain E6 plasmid p4, complete sequence.
ACCESSION   NZ_CP104543
VERSION     NZ_CP104543.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30789309
            Assembly: GCF_025369915.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Feng,A., Leigh,S.A., Akter,S., Wang,H., Pharr,T.G., Evans,J.,
            Branton,S.L., Landinez,M.P., Pace,L. and Wan,X.-F.
  TITLE     Genomic Diversity, Pathogenicity and Antimicrobial Resistance of
            Escherichia coli Isolated from Poultry in the Southern United States
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Leigh,S.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2022) Bond Life Science Center, University of
            Missouri, University of Missouri, Columbia, MO 65201-8522, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9b
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100%x
            Sequencing Technology             :: Oxford Nanopore; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/07/2023 07:35:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,147
            CDSs (total)                      :: 5,028
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 82 of 192
            Pseudo Genes (incomplete)         :: 113 of 192
            Pseudo Genes (internal stop)      :: 37 of 192
            Pseudo Genes (multiple problems)  :: 39 of 192
            Pseudo Genes (short protein)      :: 2 of 192
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104543.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E6"
                     /isolation_source="Broiler"
                     /host="Poultry"
                     /db_xref="taxon:562"
                     /plasmid="p4"
                     /geo_loc_name="USA"
                     /collection_date="2017-06-02"
                     /collected_by="USDA"
     gene            join(6272..6647,1..1193)
                     /gene="cea"
                     /locus_tag="N5O76_RS25525"
                     /old_locus_tag="N5O76_25525"
     CDS             join(6272..6647,1..1193)
                     /gene="cea"
                     /locus_tag="N5O76_RS25525"
                     /old_locus_tag="N5O76_25525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_106489667.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGAPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            1579..1716
                     /locus_tag="N5O76_RS25530"
                     /old_locus_tag="N5O76_25530"
     CDS             1579..1716
                     /locus_tag="N5O76_RS25530"
                     /old_locus_tag="N5O76_25530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(1915..2073)
                     /locus_tag="N5O76_RS25790"
     CDS             complement(1915..2073)
                     /locus_tag="N5O76_RS25790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(2743..2934)
                     /locus_tag="N5O76_RS25535"
                     /old_locus_tag="N5O76_25535"
     CDS             complement(2743..2934)
                     /locus_tag="N5O76_RS25535"
                     /old_locus_tag="N5O76_25535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2998..3321
                     /locus_tag="N5O76_RS25540"
                     /old_locus_tag="N5O76_25540"
     CDS             2998..3321
                     /locus_tag="N5O76_RS25540"
                     /old_locus_tag="N5O76_25540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3530..>3961
                     /locus_tag="N5O76_RS25545"
                     /old_locus_tag="N5O76_25545"
                     /pseudo=""
     CDS             3530..>3961
                     /locus_tag="N5O76_RS25545"
                     /old_locus_tag="N5O76_25545"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3999..4517
                     /locus_tag="N5O76_RS25550"
                     /old_locus_tag="N5O76_25550"
     CDS             3999..4517
                     /locus_tag="N5O76_RS25550"
                     /old_locus_tag="N5O76_25550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            4524..4757
                     /locus_tag="N5O76_RS25555"
                     /old_locus_tag="N5O76_25555"
     CDS             4524..4757
                     /locus_tag="N5O76_RS25555"
                     /old_locus_tag="N5O76_25555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5075..5494)
                     /locus_tag="N5O76_RS25560"
                     /old_locus_tag="N5O76_25560"
     CDS             complement(5075..5494)
                     /locus_tag="N5O76_RS25560"
                     /old_locus_tag="N5O76_25560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(5575..6012)
                     /locus_tag="N5O76_RS25565"
                     /old_locus_tag="N5O76_25565"
     CDS             complement(5575..6012)
                     /locus_tag="N5O76_RS25565"
                     /old_locus_tag="N5O76_25565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     Typing          2571..2658
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(2002..2557)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.699"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3312..4861
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagcagagcg tttgcgcctt gcgaaagcag aagaaaaagc ccgtaaagaa gcggaagcag
       61 cagaaaaggc ttttcaggaa gcagaacaac gacgtaaaga gattgaacgg gagaaggctg
      121 aaacagaacg ccagttgaaa ctggctgaag ctgaagagaa acgactggct gcattgagtg
      181 aagaagctaa agctgttgag atcgcccaaa aaaaactttc tgctgcacaa tctgaagtgg
      241 tgaaaatgga tggagagatt aagactctca attctcgttt aagctccagt atccatgccc
      301 gtgatgcaga aatgaaaacg ctcgctggaa aacgaaatga actggctcag gcatccgcta
      361 aatataaaga actggatgag ctggtcaaaa aactatcacc aagagccaat gatccgcttc
      421 agaaccgtcc tttttttgaa gcaaccagac gacgggttgg ggccggtaag attagagaag
      481 aaaaacaaaa acaggtaaca gcatcagaaa cacgtattaa ccggataaat gctgatataa
      541 ctcagatcca gaaggctatt tctcaggtca gtaataatcg taatgccggt atcgctcgtg
      601 ttcatgaagc tgaagaaaat ttgaaaaaag cacagaataa tctccttaat tcacagatta
      661 aggatgctgt tgatgcaaca gttagctttt atcaaacgct gactgaaaaa tatggtgaaa
      721 aatattcgaa aatggcacag gaacttgctg ataagtctaa aggtaagaaa atcggcaatg
      781 tgaatgaagc tctcgctgct tttgaaaaat acaaggatgt tttaaataag aaattcagca
      841 aagccgatcg tgatgctatt tttaatgcgt tggcatcggt gaagtatgat gactgggcta
      901 aacatttaga tcagtttgcc aagtacttga agattacggg gcatgtttct tttggatatg
      961 atgtggtatc tgatatccta aaaattaagg atacaggtga ctggaagcca ctatttctta
     1021 cattagagaa gaaagctgca gatgcagggg tgagttatgt tgttgcttta ctttttagct
     1081 tgcttgctgg aactacatta ggtatttggg gtattgctat tgttacagga attctatgct
     1141 cctatattga taagaataaa cttaatacta taaatgaggt gttagggatt taattattct
     1201 ttattgatat aaaaagtcct agcaatccaa atgggattgc taggaccaaa caaagtagat
     1261 tatatagcat aaataggttt aattttgcta cgggggcgtt atttaggttt tttcttctct
     1321 cgaaaaaatc tttctttatg aagttaaaag ctatgtattc aatagcatat tttgaatatg
     1381 gacatagaat agtgcttatc actattgcat atagcatctt atctgacaca aggaaataat
     1441 acccttcgct gttttttgtt ataaggtata tatatataag tgtgcagtac aggccaaata
     1501 aaatattttt tatgtagtat cttaagctca taaattaaac ctcgccatat attcttttca
     1561 ttttataagg atcgagttat gaggaaaaga ttttttgtgg gaatattcgc gataaacctc
     1621 cttgttggat gtcaggctaa ctatatacgt gatgttcagg gagggaccat cgcaccatcc
     1681 tcctcttcta aactgacggg gatcgcggtt cagtagaaaa gattaaagga tcttcttgag
     1741 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccaacgg
     1801 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     1861 gagcgcagat accaaatact gtccttctag tgtagccgta gtcgggccac tacttcaaga
     1921 actctgtagc accgtttgtg ccatcatcgc tctgctaatc cggttaccag tggctgctgc
     1981 cagtggcgtt aaggcgtgcc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2041 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2101 caccgaactg agataccaac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2161 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2221 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2281 gcgtctattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg cctgctacgt
     2341 ggccttcttc ctgttcctgg tcttttgctc acatgttctt tccggcctta tcccctgatt
     2401 ctgtggataa ctgtgttacc gtttttgtgt gagtcagtac cgctcgccgc agtcgaacga
     2461 ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg cattttctcc
     2521 ttacgcatct gtgcggcatt tcacacccgg catggcgtac ttttcataca atccgcactg
     2581 atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg tcagggctgc
     2641 gccccgacac ccgctaaaac ctgctgacgc gccctgacgg gcttgtcagc tcccggcatc
     2701 cgctcacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     2761 atccccgaaa cgtgcgaggc agctgcggta aagctcatcg gcgtggtcgt gaagcgattc
     2821 acaaatatcg gcctgttcat ctgcgtccag ttcgttgagc ttctccagca gcgttaatgt
     2881 ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttag tcactgatgc
     2941 ctccgtgtaa gggggatttc tgttcatggg gtaatgatac cgatgaaacg cgagaggatg
     3001 ctcacaatac gggttactga tgatgaacat gcccggttac ttgaacgctg tgagggtaaa
     3061 caactggcgg tatggatgcg gcgggtctgc ctgggggagc cggttgcccg ttccggaaaa
     3121 ctgccgacac tggcaccgcc gttactgcgt cagctggccg ccatcggaaa taacctgaat
     3181 cagacagccc gtaaggtgaa cagcgggcag tggtcttccg gtgaccgggt tcaggtggtg
     3241 gccgcactga tggccatcgg ggatgagctg cgccggctgc gtctggctgt cagggaacag
     3301 ggggcgcggg atgatagtta aatttcatgc caggggaaaa ggtggtggca gtggtccggt
     3361 tgattacctg ctggggaggg agcgtaaccg cgaaggcgca acggtgcttc agggtaatcc
     3421 ggaagaagtc cgggaactca tcgatgccac gccatttgcg aagaaataca cgtccggtgt
     3481 tctgtcgttc gcggagaagg agctgccgcc gggaggacgt gaaaaagtga tggcgagctt
     3541 tgagcgtgta ctgatgcccg gtctcgaaaa aaatcagtac agcatcctgt gggtggagca
     3601 ccaggacaag ggacggcttg agctgaattt tgtcattccg aacatggagc tacagaccgg
     3661 aaaacgcctc cagccgtact acgaccgcgc agacaggcct agaattgatg cctggcagac
     3721 gctggtaaat caccattacg ggctgcatga cccgaacgcc ccggagaacc gcaggacgct
     3781 gacactccct gataacctgc ctgaaacgaa acaggcgctt gctgagggcg tcacgcgagg
     3841 tatagatgca ctttaccatg ccggagagat aaaaggccgt caggatgtga ttcaggcgct
     3901 cactgaggcg gggctggaag tggtcagggt gacgcgaagc agtatcagca ttgcagatcc
     3961 gaacggcggg aagaatatca ggctgaaagg agcattttat gagcaatctt ttgcagacgg
     4021 gcgcggagtt cgagaaaaag ctgaaagaga gagccgaatc tacagagaaa atgctgaaca
     4081 acgagttcag gaggctcggc gaatctgtaa gcgaggctgt gacatcaaac gagacgaaaa
     4141 tcagagacgc tatagccctg ttcacagcct cgacagagga atcgctggaa aaacaccggg
     4201 aaggggtgaa agaggcgatg atgcagcaca ggagggacgt gttaaagctg gcagggaata
     4261 cgggcatgat gttactgggg atagtctttc tcctgtttac cgcgagtggc gggacgctct
     4321 ggtatcttgg agggaggata caggcgaacc tggaagaaat caggaagcag gaagagacat
     4381 tgcagaaact gaacgcgaag acatggggcg tggagtttgt gcaggacggg aacaggaaat
     4441 tccttgtcct tccgtacggg aaatcagcgg aggtgattcc ctttcagggg aaagagtggg
     4501 tacatctgaa ggagtgacac agagtgacag agctggaaac acatttgctg aacgccttag
     4561 agcagctgca acaggactat atgcagcggc tgagcgaatg ggagagcgcc ttcgtggaat
     4621 tgcagaagat gttttcgctt acgcaacggg acaacgcgat gctgaacgag cgggtcatgc
     4681 agttgagtca gcaggtgcag cacttgagcg agcagacaga acgcttgagc cagttataca
     4741 gcgagaactg gagataagag aggaacggct gatacaggag cgcgaacatg tgttatccct
     4801 ggaacgggag cgtcagccgg aaatacagga acgcacgctg gatggccctt cgctgggatg
     4861 gtgaaaccat gaaaaatggc agcttcagtg gattaagtgg gggtaatgtg gcctgtaccc
     4921 tctggttgca taggtattca tacggttaaa atttatcagg cgcgatcgcg gcagtttttc
     4981 gggtggtttg ttgccatttt tacctgtctg ctgccgtgat cgcgctgaac gcgttttagc
     5041 ggtgcgtaca attaagggat tatggtaaat ccacttactg tctgccctcg tagccatcga
     5101 gataaaccgc agaaatcgtg tcagccagca gcctggattt tttcggggta aggtctttgc
     5161 tgcggtaatc agtacccgca aattgagttg tgccccggag tgagtttaaa aattcctggc
     5221 tgtgaaactg ctgaatgcgt tgttgcacat cactccgtga ttttcctgtc gctttatctt
     5281 tagctcccat atcccagaac tgccgaaaaa aaggcaccat cattcgtgtt gtgtcggctt
     5341 tttgggtgga aaagttggga tcaaaaccgt catcagaagc atatacgtaa tgccgggtat
     5401 gttgttccac gctgcccttt ggggtacagg ctgtgagagc tactgttgct gttatcatgg
     5461 cgataagaca aagtttgttt ccggtacgtc tcatgagtgt ctccctacct taaagtattt
     5521 attctcggtg agttattatc cgaagactat tttatcatca gtctctgaca gcgttcagcg
     5581 accgaacaac ctggaccaga atccccgttt cttttccagt tccagtgcct gcctgagctg
     5641 ggctatctca ttttgtagct gttcccgttc tgcttcctgg cgtctgcgat ccatatcctg
     5701 tgcctgttta tcctcaagca tcagcgtcag gcattgtttc agctcattca gttcccggag
     5761 aatgcgttct gtctgctgat catgtggatt ttctgcatgt ccctcactgt gcctttctgg
     5821 tgtctcattc tgctttaatt cgccgtatgc ccggatcagt tcactggttt caaactcccg
     5881 tcgtccgtcc ctgccggtac ggtaactgac aaggcctgcg cgcatatcac ggtaaaactg
     5941 tgaacgcgat ctgcctgtca tttttagtgc gtcccggagt gtgtgccatg ccataaagtg
     6001 acagtgtccc atagatgtct catctcatag tttcagtaaa acataatgag gtctgagaac
     6061 ggtaatgttt gtgctggttt ttgtggcatc gggcgagaat agcgcgtggt gtgaaagact
     6121 gtttttttga tcgttttcac aaaaatggaa gtccacagtc ttgacaggga aaatgcagcg
     6181 gcgtagcttt tatgctgtat ataaaaccag tggttatatg tacagtattt atttttaact
     6241 tattgtttta aaagtcaaag aggattttat aatggaaacc gcggtagcgt actataaaga
     6301 tggtgttcct tatgatgata agggacaggt aattattact cttttgaatg gtgctcctga
     6361 cgggagtggc tctggcggcg gaggtggaaa aggaggcagt aaaagtgaaa gttctgcagc
     6421 tattcatgca actgctaaat ggtctactgc tcaattaaag aaaacacagg cagagcaggc
     6481 tgcccgggca aaagctgcag cggaagcaca ggcgaaagca aaggcaaaca gggatgcgct
     6541 gactcagcgc ctgaaggata tcgtgaatga ggctcttcgt cacaatgcct cacgtacgcc
     6601 ttcagcaaca gagcttgctc atgctaataa tgcagctatg caggcgg
//
