LOCUS       NZ_CP104532             1748 bp    DNA     circular CON 06-OCT-2023
DEFINITION  Escherichia coli strain E8 plasmid p6, complete sequence.
ACCESSION   NZ_CP104532
VERSION     NZ_CP104532.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30789311
            Assembly: GCF_025369695.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Feng,A., Leigh,S.A., Akter,S., Wang,H., Pharr,T.G., Evans,J.,
            Branton,S.L., Landinez,M.P., Pace,L. and Wan,X.-F.
  TITLE     Genomic Diversity, Pathogenicity and Antimicrobial Resistance of
            Escherichia coli Isolated from Poultry in the Southern United States
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Leigh,S.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2022) Bond Life Science Center, University of
            Missouri, University of Missouri, Columbia, MO 65201-8522, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9b
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100%x
            Sequencing Technology             :: Oxford Nanopore; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/06/2023 04:00:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,961
            CDSs (total)                      :: 4,843
            Genes (coding)                    :: 4,556
            CDSs (with protein)               :: 4,556
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 287
            CDSs (without protein)            :: 287
            Pseudo Genes (ambiguous residues) :: 0 of 287
            Pseudo Genes (frameshifted)       :: 107 of 287
            Pseudo Genes (incomplete)         :: 175 of 287
            Pseudo Genes (internal stop)      :: 49 of 287
            Pseudo Genes (multiple problems)  :: 43 of 287
            Pseudo Genes (short protein)      :: 3 of 287
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104532.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E8"
                     /isolation_source="Pullet"
                     /host="Poultry"
                     /db_xref="taxon:562"
                     /plasmid="p6"
                     /geo_loc_name="USA"
                     /collection_date="2017-06-05"
                     /collected_by="USDA"
     gene            complement(659..1648)
                     /locus_tag="N5O73_RS24670"
                     /old_locus_tag="N5O73_24675"
     CDS             complement(659..1648)
                     /locus_tag="N5O73_RS24670"
                     /old_locus_tag="N5O73_24675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_261246590.1"
                     /translation="MKNPELQNLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPICQWRRSLMWQARFYQSLPRIV
                     ADYPDSRWMFLTLTVRNCAIGDLGDTLSRMNTAFQRLKDRKEFRPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPGMLNGKSYVKHARWVELWRECLRVSYDPNVDVRAVKPRKPKDG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKIEVKKYRRSPTKDKTESE"
                     /name="protein rep"
     Typing          742..931
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.859"
                     /coverage="99.0"
                     /evalue="3.7200000000000003e-85"
ORIGIN
        1 cccccgaacg atggccgtcg ttgcgggggt cgcttttaac gcgaccactc cagacagaat
       61 aacttcaaag tcattttcag gcaaatcacg ctgaaaccct tgcggcacaa gggctgaggc
      121 ttatttttaa cgccagagtt atcccttaag tataaataca aggaacgcgc gcgatttttt
      181 gcccccaaaa tccacgttaa atcggacccc acaaacccca aaaacgctta tcaggtggtt
      241 gcgctcactg cgtgatcaca accacctgtc ggcagtgttt tacgggtgcg ctcgtcaatc
      301 actaaatcgg aacccactgg caagccagcg ggaccccacg atttagcgtt tgacgatttc
      361 aggaacggcg aacgctggca gattttaatc tggcggcgtg aggtgttggc agggtgtcgg
      421 ggcgcagccc tgaccaagat attttggact gtccgtcgcg tgtcggcggt actccaaaaa
      481 cacatcttgt cccgtgcgtt ttgtattacc gccatcacga tggccgcaat ccaaaacccc
      541 aggtcagggc gcagccccga caccccagag ccgactgcag gtcactgggc gaacgcggcg
      601 gaatgatgtc ggatgacgtc attttgcggc gtttgcccta tcctgcatcg cagtgttttt
      661 actctgattc ggttttatct ttcgtcggcg aacgccggta ttttttgacc tcgattttcc
      721 actcgaacgc gatgcgggaa ccgtcatcat cgccctcgga aatatcatcc ccgatcacca
      781 tgtcggcatc ggtttcctga tcgagtttca atacgtcttt gagtgcgccg ccggtcgcca
      841 caaagcggcg cttgtgcgtc tggcgggtca gttcgaggaa ccattcagga tcggcgacca
      901 tgtcggccgg cttcgtggaa tatttcaggg tctcggccac cgcgccgcgc accagttcgg
      961 cggtcgcgca ggccagcgat tcgccgtcct tcggcttccg gggtttcact gcccggacat
     1021 cgacattcgg atcgtagctg acccgaaggc actcccgcca cagctcaacc cagcgcgcat
     1081 gtttgacgta actcttcccg ttaagcatgc caggcggaac catcatcagt gtgtggaagt
     1141 gcgggtgtgc gctgccgtca ctgccgcggg tgacttccgt ggtgcggatc catccctgaa
     1201 ctggtctgaa ctctttgcga tccttcagcc gctggaaagc cgtgttcatc cggctcagcg
     1261 tatcgcccag atccccgatc gcacagttgc gcacggtcag ggtcaggaac atccagcggg
     1321 aatcggggta atccgccacg atacgcggca gggactggta gaaacgggct tgccacatca
     1381 gggagcgcct ccactggcag ataggacagt gccggacacg gcagaaatgc gcctcacgca
     1441 gcctcaggcg cgtttctccg gtttctttga gggtagacca cccgaaacgc agtaaaccgc
     1501 cgcaggaggc catacgcgcc ccgtagcgct catattcggc cgcaagcagg taaatccctc
     1561 ccacgtcgtc agaaacgctt ctgtgagcgt cccagggcgc atctgagggg gaatagtccg
     1621 tgaggttctg gagttcagga tttttcatgc gcaccgcccc atactgggcg atggctcact
     1681 tgacgtgtta tcattgccgt atcacctgtg ttgtctgtcg caaaatagaa ttcctacagg
     1741 tggtcacg
//
