LOCUS       NZ_CP104519             6776 bp    DNA     circular CON 07-OCT-2023
DEFINITION  Escherichia coli strain E4 plasmid p3, complete sequence.
ACCESSION   NZ_CP104519
VERSION     NZ_CP104519.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30789307
            Assembly: GCF_025369855.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6776)
  AUTHORS   Feng,A., Leigh,S.A., Akter,S., Wang,H., Pharr,T.G., Evans,J.,
            Branton,S.L., Landinez,M.P., Pace,L. and Wan,X.-F.
  TITLE     Genomic Diversity, Pathogenicity and Antimicrobial Resistance of
            Escherichia coli Isolated from Poultry in the Southern United States
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6776)
  AUTHORS   Leigh,S.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2022) Bond Life Science Center, University of
            Missouri, University of Missouri, Columbia, MO 65201-8522, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9b
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100%x
            Sequencing Technology             :: Oxford Nanopore; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/07/2023 07:13:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,178
            CDSs (total)                      :: 5,065
            Genes (coding)                    :: 4,771
            CDSs (with protein)               :: 4,771
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 294
            CDSs (without protein)            :: 294
            Pseudo Genes (ambiguous residues) :: 0 of 294
            Pseudo Genes (frameshifted)       :: 84 of 294
            Pseudo Genes (incomplete)         :: 206 of 294
            Pseudo Genes (internal stop)      :: 72 of 294
            Pseudo Genes (multiple problems)  :: 62 of 294
            Pseudo Genes (short protein)      :: 4 of 294
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104519.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E4"
                     /isolation_source="Pullet"
                     /host="Poultry"
                     /db_xref="taxon:562"
                     /plasmid="p3"
                     /geo_loc_name="USA"
                     /collection_date="2017-05-30"
                     /collected_by="USDA"
     gene            complement(join(6513..6776,1..1410))
                     /locus_tag="N5O79_RS25745"
                     /old_locus_tag="N5O79_25745"
     CDS             complement(join(6513..6776,1..1410))
                     /locus_tag="N5O79_RS25745"
                     /old_locus_tag="N5O79_25745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_045145925.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVSGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGRGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA
                     VSISASELSAAIAGIIAKLKKVNLKFTPFGVVLSSLIPSEIAKDDPNMMSKIVTSLPAD
                     DITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFT
                     ASIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGH
                     NAVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVAR
                     NQERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFNRFAHDPMAGGHRMWQMAGLKAQ
                     RAQTDVNNKQAAFDAAAKEKADADAALSTAMESRKKKEDNKRDAEGKLNDELEKNKGKI
                     PGLKIDQKIRGQMPERGWTEDDIKNTVSNGATGTSFDKRSPKKTPPDYLGRNDPATVYG
                     SPGKYVVVNDRTGEVTQISDKTDPGWVDDSRIQWGNKNDQ"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(1642..3861)
                     /gene="mobQ"
                     /locus_tag="N5O79_RS25750"
                     /old_locus_tag="N5O79_25750"
     CDS             complement(1642..3861)
                     /gene="mobQ"
                     /locus_tag="N5O79_RS25750"
                     /old_locus_tag="N5O79_25750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029342.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTRK
                     RGVDDAVILTPVNAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAREA
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFVAMNGKANDIKREGNALVKERDILEKEMTGLRKARPVASI
                     LSELPLMTWAEPEYRKRQLRFWKLEKQIESLLRTYRAVKERDIPARRQAFETQWNTWIS
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEYDARLKHHDNHRLSRETALAGVITGLG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGAVMVSDIREG
                     MPESHERMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPVA
                     SPLPSAHRVDVTAYAAPEQLMPVVFSTERNSRTLNLLLCKGPEEVLVGFVRQEDGLRPV
                     LALPSPDYSHLIVSTITENGVCLAGYGEAINHDADTPYPPEPHLMQFRLKGHHDRLLAA
                     VHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
                     /name="mobQ"
     gene            4040..4315
                     /locus_tag="N5O79_RS25755"
                     /old_locus_tag="N5O79_25755"
     CDS             4040..4315
                     /locus_tag="N5O79_RS25755"
                     /old_locus_tag="N5O79_25755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000091752.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEKLPRPPAKPQNH"
                     /name="hypothetical protein"
     gene            complement(4531..5424)
                     /locus_tag="N5O79_RS25760"
                     /old_locus_tag="N5O79_25760"
     CDS             complement(4531..5424)
                     /locus_tag="N5O79_RS25760"
                     /old_locus_tag="N5O79_25760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001277133.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFAYGVRILPKNIAILARFIQQNQP
                     HALYWLPFDVDRTGASIDWSDRNCPAPNITVKNPRNGHAHLLYALALPVRTAPDASASA
                     LRYAAAIERALCEKLGADVNYSGLICKNPCHPEWQEVEWREEPYTLDELADYLDLSASA
                     RRSVDKNYGLGRNYHLFEKVRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGKSIAKYTHRKFSPEGFSAVQAARGRKGGTKSKRAAVPTSARSLKPWEALGIS
                     RATYYRKLKCDPDLAK"
                     /name="replication initiation protein"
     gene            complement(5953..6096)
                     /locus_tag="N5O79_RS25765"
                     /old_locus_tag="N5O79_25765"
     CDS             complement(5953..6096)
                     /locus_tag="N5O79_RS25765"
                     /old_locus_tag="N5O79_25765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001354641.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSSAELTGLE
                     TQ"
                     /name="colicin release lysis protein"
     gene            complement(6194..6523)
                     /locus_tag="N5O79_RS25770"
                     /old_locus_tag="N5O79_25770"
     CDS             complement(6194..6523)
                     /locus_tag="N5O79_RS25770"
                     /old_locus_tag="N5O79_25770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="WP_000182536.1"
                     /translation="MTNKLFEHTVLYDSGDAFFELKGNASMKLSPKAAIEVCNEAAKKG
                     LWILGIDGGHWLNPGFRIDSSASWTYDMPEEYKSKIPENNRLAIENIKDDIENGYTAFI
                     ITLKM"
                     /name="colicin transporter"
     BGC             1..6410
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          3504..3862
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.889"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3863..4033
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.93"
                     /coverage="97.0"
                     /evalue="5.74e-73"
     Typing          complement(4532..5424)
                     /name="rep_cluster_2150"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.275"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1646..3861)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.587"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgcccccta attttctgat ctatttttag acctggtatc tttcctttat ttttttccaa
       61 ctcatcattt agtttgcctt cagcatctct tttattatcc tctttcttct tcctgctttc
      121 catcgcagta cttaatgcag catcagcatc tgccttctct tttgcagcag catcaaatgc
      181 agcctgctta ttatttacat ccgtctgcgc ccgctgagct ttaagcccgg ccatttgcca
      241 cattctgtga ccgccagcca ttgggtcatg ggcaaatcga ttaaattgtt ttatttcagc
      301 tattgcatca gcaagagttt tattcgctgc atcaagttcg cttttacgcg aattataaac
      361 ctgaacagct ttagcctgtc gctcctgatt tctggcaaca tcttcatttg cctgattcag
      421 ctctgcacgc gcgcgttcat aatttcgctc agccgcttca accggatgcg tagcatccca
      481 ttcctgctga cggcgatttt cttcatcctg acgttgtttt acctggtcag gactaagaac
      541 atcactcact gaaacatata cggcattatg accgctgtcc ttcgggaatc gaataactgc
      601 atccctggta ttaccaccct gagtaaatcc tgccgggcga acatccttat cagtattatt
      661 tgtaacacct gggcttaatg tctgtactgc tggcgtactg ttattaactg aaatattcag
      721 aacaggtgca cctggaattg atgccgtaaa aacacctgga cgttcggtag gttttgcatc
      781 aaccaccgga acactcatcg gaacacctga aacaaccgaa atattctgtc gttcgtcttt
      841 tacatcatca acaacacgaa catttacgtt tactgttgcc ttatcgagag gtaatgaact
      901 gacaggtgat tcagtaatat catctgcggg taatgacgtc acaatctttg acatcatatt
      961 ggggtcatct ttcgctattt ccgacggaat taatgaagat aagacaaccc caaaaggagt
     1021 gaatttaaga tttacttttt ttaatttagc aataatacca gcaatagctg ccgataattc
     1081 gcttgcagaa atactgacag ccagaccgcc agctcctgga gtggaaagtg ccggaaaacc
     1141 aaatgccact ggcgcagcta ctgctgacaa attaccgcct gttcccgagc caccaccgga
     1201 attgccatta cccccgccat taccacgacc ggagccacct ccccagtgaa tgccgctacc
     1261 ggaaccacca ccccacgggt tattttccga actccatcct gaaccatcag aagcaccacc
     1321 acttacacca agcccggtcg ggccaccatt aatgttacca cttgtgctat gcgcgcccgt
     1381 gttatggccg cgtccatctc caccgctcat aaaagtccct cttttaagcg ttaaaacaat
     1441 taattacatg attaatactg tacataaaac cactggtttt atgtacagat caaaaagtac
     1501 ccgtcatcgt tttttctgtc aagttcaggt ggaaaatttt ttagaagtca ggagggataa
     1561 cacttagaaa acgcagagcg gaccacaaaa aattttgtgg cccgtgatgt gttggtaggg
     1621 ttcggggggc agccccggat atcacatact cataccccgg aagtgcccgg ggcggcgttg
     1681 ttgttgcctg tgctgttcgt cccgcttcca ctcatgccag gtctgattaa aaccgagttg
     1741 acggaagaga taatccggca tctcttccgg tttgtggaca gcagccaaaa gcctgtcatg
     1801 atggcctttg agccggaact gcatcaggtg cggttccggt gggtacggag tatccgcatc
     1861 atggtttata gcttctccgt aacctgccag gcataccccg ttctccgtga tggtgctgac
     1921 tatcagatga ctgtaatccg gcgacggaag cgcaagaacg ggacgcagcc cgtcctcctg
     1981 gcgcacaaat ccgacaagca cttcctccgg ccctttgcac aacagcagat tcagcgtcct
     2041 gctgtttcgc tctgtcgaaa atacaacggg catgagttgc tccggtgccg cataagcggt
     2101 gacgtccacc cggtgcgcag acggcagcgg agaggctacg ggcctgcggt cgtcgctgaa
     2161 ataaaaatta tccggccagt gatgcggcag cgccggaaaa ccgtaagagg gccacccctg
     2221 gcgcatctgt tccagtacat gccggaccgt cgcaccggaa tacattctca cacgctcagg
     2281 ccgcgccatc ctctcatgag attctggcat tccctcacgt atatccgaga ccatcacagc
     2341 cccgcctggc gacaggtaaa cattcaccgg gtcatgaaca ctctgggggt aatgcgccag
     2401 ctcatcaccc cataccgtga attctccggc tctgttactc agcatcatgt agcgggttat
     2461 cctgcccgtt cccggctctc tggcacgccc cagccccgta ataaccccgg ctaatgccgt
     2521 ttcacggctc aggcgatggt tatcatggtg tttcagccgt gcgtcatatt cctgttcctt
     2581 ccggcgcgct tccgcttcgg cttcctccct gcgccgctcc gcttcccgtg ctgacagttt
     2641 ttctttcagc tctgccattc ccggcgaaat ccacgtattc cactgcgttt caaaggcctg
     2701 acgacgggcg ggaatgtccc gttctttcac ggccctgtag gtgcggagaa gagattcaat
     2761 ctgtttctcg agtttccaga agcggagttg tcttttgcgg tattccggtt cagcccatgt
     2821 catcagggga agctctgaca ggatggacgc taccggacgg gcttttctga gtccggtcat
     2881 ctctttttcg agaatatccc gctctttcac cagcgcattc ccctcacgtt tgatgtcgtt
     2941 tgccttcccg ttcatcgcca cgaactccgg tgtcatctcc catgcgttcc cgcattcctg
     3001 cggcagtgtg gcaatgaatt cccgcagggt atccgctgag gcattcgcgg cgggaatggt
     3061 cagattaagg gcttccgctg cacgacgtgc ggcatcgctg agttcccgtt tccgcacgtt
     3121 ctcctgtacc tggatgactg ccagccttgt gcgggaaacc tgtatttcca ggttaaggct
     3181 gttaatcaga cggttctgct caccgcgttc tgtttccatg ccccgttttt ccatcgcaca
     3241 ggcagccttt ccgaggtgaa ggccgggcgc tttctccagt ccctgacgct cgtatgaacg
     3301 atggtctatc tcttcctggt agccggcgtt cgcaagcgct ctgttcgcat ggtcagccca
     3361 tgatgcgcgc cacgtctcgg cgagtgcccg gtcgttccag tccctgacct ttcctgcgaa
     3421 tcccgtttcc ccgacagctc tcgtggtcag catgatgtgt gcatgggggt tggtccggtc
     3481 catatgatgg aacgccacat cggcaatcat gccccgactg acaaagtttt cccggacaaa
     3541 agcgagaacg gcctcccgtg cggcctcgcg ggaaatctca cggggaatgg cgagttcaat
     3601 ctcccttgcc agctgggagt tccggcgctg ttcggctttc tcgaccgcat tccagagaac
     3661 ggaacggtca ccacaccagg acggtgcatt cacaggggtg agaatgaccg catcatcaac
     3721 gccgcgttta cgggtgtaat cgtgggttaa tccggtacga tcatcgggta tgacagagcc
     3781 ggaacggtag gcagcagaag caacggcact gtaaccgttt tttcgcgaaa tgattttcat
     3841 gctgagatga taaatcgcca tttcttccgt aatgcctttt tgccgttttc tttcagcccc
     3901 gcagggcgca cacaatcgaa acgaagtgaa gattgtataa gtgcgcactt cgtgatgtgc
     3961 cgtgcattat gccagttcag ttttgcaact accagtgacg cgctaaaata cgatttttca
     4021 ccgcgcacgg agtcacgaaa tgagcagaac actcgaacag aagattgccg aagctgaggc
     4081 cagattacag cgcctgaagg cgaagagccg gagcctggac acggcgcaaa aagtcattgt
     4141 gggggctgcc atgctggcga gggtacggag gccggaagaa gcgcaactgc gtgcgttcct
     4201 gctccagttt ctgcgaaagg aagtcacaag acaggcggat gtgaaccgac tacaaccgct
     4261 gattaacgag ctggagaagc tacccagacc accggcgaaa ccgcagaacc actaagcccc
     4321 ggcgcgtaag tgcccatgca ccaccggacg cctttgcgct tcctcgctca ttcgctcgct
     4381 acgctcggtc atgagactgc ggcgagcgct accacctcaa tcatgcggaa agccacgcca
     4441 ttacggggca aaacggagga aatcgcaaga aagatcactg ctcaaaaaga cgccaaaagg
     4501 gcgctgttat ctgataaggc ttatctggtc tcattttgcg agatctggat cacattttaa
     4561 ttttcggtag tacgtcgctc gactgatccc taatgcctcc cacggcttca gcgaacgtgc
     4621 tgatgtagga actgctgcgc gcttagattt agttccgccc ttgcgacctc gagcggcctg
     4681 tacagcggaa aatccctctg gtgagaattt cctgtgtgta tatttcgcaa tgctcttgcc
     4741 aatagcccga cattcagccg gagaaagcgg aacgggaagc gatgcgttgt acatttcgac
     4801 acgctggatc acggcatcaa gccattgtga gaatacaggc cagccctgac gaatcgctct
     4861 gtaggcccat ttacggacct tttcgaacag atggtagttt cgccccagcc cgtaattttt
     4921 atcgacgcta cggcgcgctg aggcgctcaa atcgagataa tcagccagtt cgtcgagagt
     4981 gtagggttcc tcgcgccatt ccacttcctg ccattcaggg tggcacggat ttttgcagat
     5041 caggccgctg taattcacat ccgcgcccag tttttcacac aacgcacgct caatagcggc
     5101 agcgtatctg agcgccgaag ccgatgcatc cggcgcagtt ctcacaggaa gggcgagcgc
     5161 gtagagcaga tgcgcgtgcc cgttacgggg attttttacg gtgatgttcg gggccggaca
     5221 attccggtcg ctccagtcga ttgatgcccc cgtccggtcc acgtcaaagg gaagccagta
     5281 cagtgcatgt ggctggttct gctggatgaa acgggcaaga atggcaatgt ttttcggcag
     5341 aatgcgaacg ccgtacgcga aatcgttcgt acagtacggt ttgtgcggta atttttccct
     5401 gaagcgttga agtacggcgc tcatgtgata cgatttctct cggtacacag tttaactaac
     5461 cgcgctttcc catcgggaaa cgcggaaccc ggcgccctgt aatttcgtga tcaatcttgg
     5521 cggtgagagc acgatttacg gggcgtttgt ttttcagtcg cttacactac acccaacggc
     5581 aaatcctgtc gaaccttccc gaaaacccga tccatttacc ataactcccg ttgtacgtac
     5641 cctctaaaac gctctcagac gcgttctgac gcaaaaaacg caaaaagaca aggaaacacc
     5701 aggctgaacc agaaaaatgc gtcagaatcg tttttagggc gctgcataac tatgcacgaa
     5761 agcgatgcgc gatcggtttc gaccgttccg aatggcgaac aggtcaaaat gacaagtttt
     5821 ctcccaccgg atggaaggtt ctgcacaggg attttagatc ccatctcccg catggaattt
     5881 ttgtgacatt tcaaaaaaaa gcgcatcaga atcagcctga tacgctttgt tcttgtcaaa
     5941 gaggatttcg ggttactgcg tttctaatcc ggtcagttca gctgaggatg acggggatac
     6001 agtcccgccc tgaacatccc ggatatagtt tgcctgacat gcagcaagaa taatgactgc
     6061 aagaagcaat aaaataatcc ctgttatttt tttcatactg ttccttgtat atatggactc
     6121 aggcattcat ccataaaaac ctgaaatcca aaggttgaaa tcatccatga aacataaaat
     6181 tctataacac tatttacatc tttaacgtga taatgaaagc agtgtatcca ttctcaatat
     6241 catctttaat attttcaata gccaatctat tattttcagg gatttttgat ttgtattcct
     6301 ccggcatatc atatgtccat gatgctgaac tatctatcct gaatccagga ttcagccaat
     6361 gcccaccatc aatgcccaaa atccataagc cttttttcgc tgcttcatta caaacttcta
     6421 ttgcggcttt tggtgataac ttcatagaag catttccttt taattcaaaa aaggcatcac
     6481 cactatcata taacaccgta tgttcaaata atttattggt catttttatt tccccattga
     6541 attctcgaat cgtccaccca acccggatcc gtcttatcac taatctgagt aacctcacca
     6601 gtacgatcat tgacaacgac atatttacca ggagaaccat acactgttgc tggatcattt
     6661 cgacctaaat agtctggagg tgttttcttc ggactacgct tatcaaaaga tgtaccagtt
     6721 gcaccgttag atacggtatt tttgatatca tcctctgtcc atcctctttc tggcat
//
