LOCUS       NZ_CP104513             6268 bp    DNA     circular CON 06-OCT-2023
DEFINITION  Escherichia coli strain E2 plasmid p4, complete sequence.
ACCESSION   NZ_CP104513
VERSION     NZ_CP104513.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30789305
            Assembly: GCF_025369675.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Feng,A., Leigh,S.A., Akter,S., Wang,H., Pharr,T.G., Evans,J.,
            Branton,S.L., Landinez,M.P., Pace,L. and Wan,X.-F.
  TITLE     Genomic Diversity, Pathogenicity and Antimicrobial Resistance of
            Escherichia coli Isolated from Poultry in the Southern United States
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6268)
  AUTHORS   Leigh,S.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2022) Bond Life Science Center, University of
            Missouri, University of Missouri, Columbia, MO 65201-8522, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9b
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100%x
            Sequencing Technology             :: Oxford Nanopore; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/06/2023 04:07:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,557
            CDSs (total)                      :: 5,428
            Genes (coding)                    :: 5,107
            CDSs (with protein)               :: 5,107
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 321
            CDSs (without protein)            :: 321
            Pseudo Genes (ambiguous residues) :: 0 of 321
            Pseudo Genes (frameshifted)       :: 87 of 321
            Pseudo Genes (incomplete)         :: 239 of 321
            Pseudo Genes (internal stop)      :: 48 of 321
            Pseudo Genes (multiple problems)  :: 47 of 321
            Pseudo Genes (short protein)      :: 4 of 321
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104513.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E2"
                     /isolation_source="Backyard chicken"
                     /host="Poultry"
                     /db_xref="taxon:562"
                     /plasmid="p4"
                     /geo_loc_name="USA"
                     /collection_date="2017-05-25"
                     /collected_by="USDA"
     gene            complement(join(5717..6268,1..588))
                     /gene="dcm"
                     /locus_tag="N5O74_RS27580"
                     /old_locus_tag="N5O74_27580"
     CDS             complement(join(5717..6268,1..588))
                     /gene="dcm"
                     /locus_tag="N5O74_RS27580"
                     /old_locus_tag="N5O74_27580"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131511.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="WP_000131512.1"
                     /translation="MTDNIAATIKGKRERLHMTQKEFADALGLSKYGDRTIRRWERGET
                     KPTGAELKAVMDFPDTPPYPNNENGRYRMIDLFAGIGGTRLGFHQTNAVNVVFSSEWDK
                     FAQKTYHANYGDFPDGDITKIDEKDIPDHEILVGGFPCVAFSQAGLKKGFNDTRGTLFF
                     DIARIIKEKKPHAFLLENVKNLLGHDKGRTFSIIKNTLEELNYTVYYNIFAAKDFGVPQ
                     NRERIYIVGFNKEKVRNHEHFTFPTPLKTKTRVGDILEKSVDNKYTLSDALWNGHQRRK
                     LVNAAAGKGFGYGLFNENSPYTNTISARYYKDGSEILIEQKGSNPRKITPREASRLQGF
                     PSDFIIPVSDTQAYKQFGNSVAVPVINAIAEKIISTLDS"
                     /name="dcm"
     gene            659..1615
                     /locus_tag="N5O74_RS27585"
                     /old_locus_tag="N5O74_27585"
     CDS             659..1615
                     /locus_tag="N5O74_RS27585"
                     /old_locus_tag="N5O74_27585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640333.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="WP_224985182.1"
                     /translation="MKKLSSRYKRLFFMQRLSPGEFKTLISKERKSHFITPFALVYKTF
                     CDLGYDQKNSDYFLNNPSEYIIAMRKNCWKEFEPFEKEFTTRMLSYLIDEERIKDMSPY
                     DAIRDFTMEYPTHIYDLALSNTQSRRSRAGKEFESILELLMMGAGIPVDVQGAIGKSFF
                     QKNQIGKLVDLVMPGVVQYTSNKRNTMLISAKTTLRERWQEVPEEVNRTGIREMYLATL
                     DDSFSEETINILYEANVVVVTTIENKNFKYKNNNRVLTFEDMLQSAMELSRKWNNVSYT
                     DSEKEEIQQSILKQIEKYSDFPYVVNYYRNRLSALVD"
                     /name="type II restriction endonuclease"
     gene            complement(1807..1968)
                     /locus_tag="N5O74_RS27840"
     CDS             complement(1807..1968)
                     /locus_tag="N5O74_RS27840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596883.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRARFI
                     QMLTEFLK"
                     /name="replication initiation protein"
     gene            complement(2638..2829)
                     /locus_tag="N5O74_RS27590"
                     /old_locus_tag="N5O74_27590"
     CDS             complement(2638..2829)
                     /locus_tag="N5O74_RS27590"
                     /old_locus_tag="N5O74_27590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2893..3216
                     /locus_tag="N5O74_RS27595"
                     /old_locus_tag="N5O74_27595"
     CDS             2893..3216
                     /locus_tag="N5O74_RS27595"
                     /old_locus_tag="N5O74_27595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001749507.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3425..>3856
                     /locus_tag="N5O74_RS27600"
                     /old_locus_tag="N5O74_27600"
                     /pseudo=""
     CDS             3425..>3856
                     /locus_tag="N5O74_RS27600"
                     /old_locus_tag="N5O74_27600"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3894..4412
                     /locus_tag="N5O74_RS27605"
                     /old_locus_tag="N5O74_27605"
     CDS             3894..4412
                     /locus_tag="N5O74_RS27605"
                     /old_locus_tag="N5O74_27605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001749510.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            4419..4652
                     /locus_tag="N5O74_RS27610"
                     /old_locus_tag="N5O74_27610"
     CDS             4419..4652
                     /locus_tag="N5O74_RS27610"
                     /old_locus_tag="N5O74_27610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_004026237.1"
                     /translation="MTELEIHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            5135..5578
                     /locus_tag="N5O74_RS27615"
                     /old_locus_tag="N5O74_27615"
     CDS             5135..5578
                     /locus_tag="N5O74_RS27615"
                     /old_locus_tag="N5O74_27615"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001605388.1"
                     /translation="MTSGIFLSREDLMLLSDQAKEEVLALATGDSVSGVYEESEDGPTD
                     LSSLQAERLVRGLSDKSRTVLKAILESEDGERGFWCKSLAKELGFDVSELSGVWSGLTR
                     RTRTVSDDSEAYLISWAWDDDRDDYYGTLHPTTYRNCKKAMNF"
                     /name="hypothetical protein"
     Typing          2468..2553
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.851"
                     /coverage="100.0"
                     /evalue="3.99e-36"
     Typing          complement(1897..2446)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.756"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2864..3210
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.103"
                     /coverage="98.0"
                     /evalue="1.36e-63"
     Typing          3207..4756
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.802"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tattgaaaaa gttctacctt tatcatgtcc tagcaagttt tttacatttt caagtaaaaa
       61 agcatgaggt tttttttcct ttattatcct tgctatatca aaaaacaaag ttcctcttgt
      121 atcattaaat ccttttttga gccctgcttg agagaatgct acgcaaggaa acccaccgac
      181 aagaatttca tgatcaggaa tatctttttc atcgattttt gttatgtccc catcagggaa
      241 atcaccataa tttgcatgat atgtcttttg agcaaactta tcccattcgc tggaaaaaac
      301 aacatttaca gcgttagttt gatgaaatcc aagtctggtt ccaccgattc cagcaaataa
      361 atcgatcatg cgatatctgc cattctcgtt gtttgggtaa ggtggtgtgt ctggaaagtc
      421 cataactgct tttagttccg ccccagtagg ttttgtttcc ccgcgctccc atcttcttat
      481 ggttctgtct ccatattttg aaagaccaag ggcgtctgca aactcttttt gagtcatatg
      541 aagtctttct ctttttcctt tgattgttgc tgcgatatta tcagtcatgc atgtctacca
      601 gaattaagtg ttggttttag gacaatttgt cctgttttga ttccaattat gaacgtgcat
      661 gaaaaaactt tcaagtagat ataaaaggtt attttttatg caaagattga gtcctggtga
      721 atttaaaact ctaatttcta aagagagaaa gtcgcatttt attacgccat ttgctttggt
      781 ttataaaaca ttttgtgatt tagggtatga tcaaaaaaat agtgattatt ttttgaataa
      841 tccaagtgaa tatataattg caatgagaaa aaactgttgg aaagaatttg aaccatttga
      901 aaaagaattc acaacaagaa tgttatcata tcttattgat gaagaaagaa taaaagatat
      961 gagtccttat gatgccattc gtgattttac gatggaatac cctacacaca tatatgattt
     1021 ggcactgtct aacactcaat ccagacgtag cagggcagga aaagagtttg agtctattct
     1081 tgagctatta atgatggggg caggaattcc tgttgatgtg cagggggcta ttgggaagtc
     1141 attttttcaa aaaaaccaaa taggtaaact tgtagatctt gttatgccag gtgtagtcca
     1201 gtacacatct aacaaaagaa atacaatgtt gatttccgct aaaacaacgc tgagggagcg
     1261 atggcaagaa gtacctgaag aggttaatag aacaggaatt agagaaatgt atctagctac
     1321 actagatgat tcttttagcg aggagacaat aaatatatta tacgaggcta atgtggtcgt
     1381 tgtaacgacg atagaaaata aaaatttcaa atataaaaat aataataggg tgctaacctt
     1441 tgaagatatg ctacagtcag caatggaatt atcaagaaag tggaataatg taagttacac
     1501 agactctgaa aaagaggaaa ttcaacagtc aatattaaaa caaatagaaa aatatagtga
     1561 tttcccttat gttgttaatt attatagaaa taggcttagt gctcttgttg actaaaaaac
     1621 taaaaggatc tcttgagatc ctttttttct gcgcgtaatc ttggacctgt aaacgaaaaa
     1681 accaccctgg caggtggttt tttcgaaggt tagctaatcc tggcagatta tctaaccgag
     1741 gtaatctggc ttcagcagag cacagatacc aaatactgtc cttccagtgt agccgtagtt
     1801 aggccatcac ttcaagaact ctgtaagcat ctggataaat ctcgctctgc taatccggtt
     1861 accagtggct gctgccagtg gcgttaaggc gtgtcttacc gggttggact caagacgata
     1921 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     1981 ggagcgaacg acctacaccg ggccgagata ccaacagcgt gagctatgag aaagcgccac
     2041 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2101 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2161 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggga
     2221 aaacgccagc ggcgcggcct tttgttgccc tttgcctttt tactcacatg ttctttccgg
     2281 ccttatcccc tgattctgtg gataaccgta tttccgcctt tgagtgagcc ggcaccgctt
     2341 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgccgga
     2401 agctgcattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     2461 gtacaatctg cactgatgcc gcatggttaa gccagtatac actccgctat cgctacgtga
     2521 ctgggtcagg gctgcgcccc gacaccccct aaaacctgct gacgcgccct gacgggcttg
     2581 tctgctcccg gcatccgctc acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     2641 gaggttttca ccgtcatccc cgaaacgtgc gaggcagctg cggtaaagct catcggcgtg
     2701 gtcgtgaagc gattcacaaa tatcggcctg ttcatccgcg tccagttcgt taagcttttc
     2761 cagcagcgtt aatgtctggc ttctgataaa gcgcgccatg ttaagggctg ttttttcctg
     2821 tttagtcact gatgcctccg tgtaaggggg atttctgttc atggggtaat gataccgatg
     2881 aaacgcgaga ggatgctcac gatacgggtt actgatgatg aacatgccag gttactggaa
     2941 cgctgtgagg gtaaacagct ggcggtatgg atgcggcggg tctgcctggg tgagccggtt
     3001 gcccgttccg ggaagctgcc gacactggca ccgccgttac tgcgtcagct ggctgccatc
     3061 gggaataacc tgaatcagac ggcgaggaag gtgaacagcg ggcagtggtc ttccggtgac
     3121 cgggttcagg tggtggccgc actgatggcc atcgagagag agttgcgcag cttacggcag
     3181 gtcgtcaggg aacagggagc gcgggatgat tgttaaattt catgccaggg gaaaaggcgg
     3241 tggcagtggt ccggttgatt acctgctggg aagggagcga aaccgggaag gcgcaacggt
     3301 gcttcagggt aatccggaag aagtccggga actcatcgat gccacgccat ttgcgaagaa
     3361 atacacgtcg ggtgttctgt cgttcgcgga gaaggagctg ccgccgggag ggcgtgaaaa
     3421 ggtgatggcg agttttgagc gtgtactgat gcccggtctc gaaaagaatc agtacagcat
     3481 cctgtgggtg gagcaccagg acaagggacg gcttgagctg aattttgtca ttccgaacat
     3541 ggagctacag accggaaaac gcctccagcc gtactacgac cgggcagaca ggccgagaat
     3601 tgatgcctgg cagacgctgg tgaatcacca ttacggactg catgacccca acgccccgga
     3661 gaaccgccgg acgctgaccc ttcctgataa cctgccggaa acgaaacagg cacttgctga
     3721 gagcgttaca cgcggtatag acgcacttta ccatgccgga gagataaaag gccgtcagga
     3781 tgtgattcag gcgctcacag aggcggggct tgaagtggtc agggtgacgc gaagcagtat
     3841 cagcattgca gacccgaacg gcgggaggaa tatcagactg aaaggagcgt tttatgagca
     3901 atcttttaca gacgggcgcg gagttcgaga aaaagctgaa agagagagcc gaatctacag
     3961 agaaaatgct gaacgacgag ttcaggaagc tcggcgagtc tgtcagcgag gctgtgacct
     4021 caaacgagac gaaaatcaga gacgctatag ccctgttcac agcctcgaca gagaaatcgc
     4081 tggaaaaaca ccgggaaggg gtgaaagagg cgatgatgca gcaccggaag gacgtattaa
     4141 agctggcagg gaatacgggc atgatgttac tggggatagt ctttctcctg tttaccgcga
     4201 gtggcgggac gctctggtat cttggcggac ggatacagac gaacctggaa gaaatcagga
     4261 agcaggaaga gacattgcag aaactgaacg cgaagacatg gggcgtggag tttgtgcagg
     4321 acgggaacag gaaattcctt gtccttccgt acgggaaatc agcggaggtg attccctttc
     4381 aggggaaaga gtgggtacat ctgaaggagt gacacagagt gacagagctg gaaatacatt
     4441 tgctgaacgc cttagagcag ctgcaacagg actatatgca gcggctgagc gaatgggaga
     4501 gcgccttcgt ggaattgcag aagatgtttt cgcttacgca acgggacaac gcgatgctga
     4561 acgagcgggt catgcagttg agtcagcagg tgcagcactt gagcgagcag acagaacgct
     4621 tgagccagtt atacagcgag aactggagat aagagaggca cggctgatac aggagcgcga
     4681 acatgcgtta tccctggaac gggagcgtca gccggaaata caggaacgca cgctggatgg
     4741 tccttcgctg ggatggtgaa atcatgaaaa atggcagctt cagtggatta agaggggtta
     4801 atgtggcctg taccatctgg ttgcataggt attcatgcgg ttaaaattta tcaggcgcga
     4861 tcgcggcagt ttttcgggtg atttgttgcc atttttacct gtctgctgcc gtgatcgcgc
     4921 tgaacgcgtt ttagcggtgc gtgtaatgcg acgttatggt aaatccgggc tgttgagtgt
     4981 tgacctctca ctatcgcaac ccagttaatg cggtaaatca aacacaacta gctgttaatt
     5041 gatgttagtt gtattgactt gtcacttgtc ttgttgacaa ctatcttgtc tatgtttatt
     5101 ctccttttat cttaacaaga caatggagtg tttcatgact agtggtattt tcttatcccg
     5161 tgaagatttg atgttgttat cagaccaagc aaaagaagaa gtgctggcat tggctactgg
     5221 ggattcggtt tctggtgttt atgaggagtc ggaagatggg cctactgatc tttcctcgct
     5281 tcaggcagaa aggcttgttc gtggtttaag tgataagtcg cgaacagtgc tgaaagctat
     5341 tcttgaaagc gaggatggtg agcgaggatt ctggtgcaag agcctcgcaa aggagcttgg
     5401 ttttgatgtc tctgagcttt caggtgtctg gtcagggctt acacgcagaa ctcgcacggt
     5461 aagtgatgat tctgaggctt atttgattag ctgggcttgg gatgatgacc gtgatgatta
     5521 ctatggaacg ctacatccaa caacctatcg caactgcaag aaagctatga acttttgatt
     5581 gttctcgctg tgatactttc cctgtgagcc cgccatgtgc gggctttttt cattggttcg
     5641 tgtagtgaga cgttatggta aatccatatg gggggggaat tggattctgg gtcgcattgt
     5701 gtccttatga cttagtttat gaatctaagg tcgatatgat tttttctgct attgcattga
     5761 taacagggac tgcgactgaa ttcccaaatt gcttataagc ctgtgtatcc gatacaggga
     5821 taataaaatc actaggaaac ccttgaagtc ttgacgcttc ccgcggagtt atttttcttg
     5881 ggttacttcc tttttgctct attaatattt cgctaccatc tttatagtac ctcgcactta
     5941 tggtattagt atatgggcta ttttcattaa ataaaccata cccaaagcct ttaccggctg
     6001 ctgcatttac aagtttcctg cgttggtgtc cattccagag cgcatctgat agcgtatatt
     6061 tattgtctac gctcttttca agaatatctc caactcttgt ttttgttttt aatggggttg
     6121 gaaaagtaaa atgttcatga tttcttactt tttctttatt aaagcctaca atgtatatgc
     6181 gttctctatt ttgtggaaca ccaaagtctt ttgcagcgaa gatattgtaa tacacagtgt
     6241 aattcaattc ttcaagtgtg ttttttat
//
