LOCUS       NZ_CP104469             4863 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain 80014967 plasmid pE80014967-2,
            complete sequence.
ACCESSION   NZ_CP104469
VERSION     NZ_CP104469.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30506756
            Assembly: GCF_025369895.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   liu,z., hang,x. and liu,l.
  TITLE     Sequencing of plasmid related to gene in Enterobacter cloacae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   liu,z., hang,x. and liu,l.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2022) Clinical Laboratory, The Second Affiliated
            Hospital of Anhui Medical University, 678 Furong Road, Shushan
            District, Hefei, Anhui 230601, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: FALCON-Unzip v. v0.3.0.
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 186.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_025369895.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:45:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,691
            CDSs (total)                      :: 4,577
            Genes (coding)                    :: 4,455
            CDSs (with protein)               :: 4,455
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 29 of 122
            Pseudo Genes (incomplete)         :: 93 of 122
            Pseudo Genes (internal stop)      :: 16 of 122
            Pseudo Genes (multiple problems)  :: 15 of 122
            Pseudo Genes (short protein)      :: 1 of 122
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104469.1.
            Bacteria and source DNA available from anyidaerfuyuanjianyanke.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="80014967"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pE80014967-2"
                     /geo_loc_name="China:Anhui"
                     /collection_date="2021-05-02"
     gene            complement(406..672)
                     /locus_tag="NYP10_RS01100"
                     /old_locus_tag="NYP10_01100"
     CDS             complement(406..672)
                     /locus_tag="NYP10_RS01100"
                     /old_locus_tag="NYP10_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072050235.1"
                     /translation="MSVYCVSYDLNQAGQNYNALYDELKKSPGWCHPLDSTWLISTGET
                     AQQLSDRLRRHLDNNDTLLVIGVTKEYAGWLTQDTWDWMRTDI"
                     /name="hypothetical protein"
     gene            1367..2665
                     /locus_tag="NYP10_RS01105"
                     /old_locus_tag="NYP10_01105"
     CDS             1367..2665
                     /locus_tag="NYP10_RS01105"
                     /old_locus_tag="NYP10_01105"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_097414638.1"
                     /translation="MMVNFSLSNAFSFMDAQNMSMLAVSSAKDDINLANVVSVSDVRIL
                     KSALIFGANASGKSNLMKTISLMKDIVLHSVQAIESSITKNVIPFLLDEDGPNKPSEME
                     MTFISDGVKYRYGLSIFQGKIKEEWLYYTPKSRETILFERDGLSIEVNKAAFSESNLFI
                     KEGIIQKTREDVPFVSVVAGFNGEHSKRVVNWFNNIVFLSGSNEGMFTAITMKLINESE
                     DFKAWLKKILPAFQINDIYLDEVAPDFPFGNIKTDDDNLKNLFSSIQNFAKNQKVFSLR
                     VVKKTASGEISVPIDFESEGTKKIVYLLGPIYNAMINGNLLLVDEFDSKFHTLLSKFLF
                     RIFHAQSEGLSQIIAAVQDVNLMNTDCFRRDQIWFVDKDIKSGSSHLYSLVEYKEKQRT
                     LKSSYGHDYLSGAFDAIPLFDNLDEFEKLMPEG"
                     /name="ATP-binding protein"
     gene            2738..3106
                     /locus_tag="NYP10_RS01110"
                     /old_locus_tag="NYP10_01110"
     CDS             2738..3106
                     /locus_tag="NYP10_RS01110"
                     /old_locus_tag="NYP10_01110"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045626380.1"
                     /translation="MKTTDRFISVSFRYFHNVDDIGQSIDSWRVDGSLPDLIEKLVYLT
                     ANGITKVSSDGIFTNYTRFPDSAVNDFKCPDEISVDENWGVIKNIGGQKRRVAGFLKDN
                     VFYIVFFDKDHRFWKSRK"
                     /name="hypothetical protein"
     gene            complement(3343..3456)
                     /locus_tag="NYP10_RS01115"
                     /old_locus_tag="NYP10_01115"
     CDS             complement(3343..3456)
                     /locus_tag="NYP10_RS01115"
                     /old_locus_tag="NYP10_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            3872..4063
                     /locus_tag="NYP10_RS01120"
                     /old_locus_tag="NYP10_01120"
     CDS             3872..4063
                     /locus_tag="NYP10_RS01120"
                     /old_locus_tag="NYP10_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_045265536.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELALDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4063..4485
                     /locus_tag="NYP10_RS01125"
                     /old_locus_tag="NYP10_01125"
     CDS             4063..4485
                     /locus_tag="NYP10_RS01125"
                     /old_locus_tag="NYP10_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_045265535.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGVNSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(3344..3751)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.484"
                     /coverage="99.0"
                     /evalue="1.3e-157"
ORIGIN
        1 gtggtgagtc gtccggcgac ttgccataac gtgattgccg gccgtcgacg aacccgattt
       61 ctgaacgtgc tgaaaaccgg ttttccggct gcgccggcaa cgcttcacgg ccacgcaata
      121 aattgcgtcc ccgttcaccg ttttcggatg ggaagaacgc cggaaacagg acttaccctg
      181 gctgaatggc gtgaattttc gcgctatgca cttgcgtgca tactcatgca tgccgtaaaa
      241 acagagcctg cgcgtttctg gcgggttttc gggtggtttg ttgcctgttt taccggtttc
      301 ccgtcagaaa cgccctgagg ccgttttagc ggtgcgtaca attagggggt tatgttaaat
      361 ccacaccagc tacagacgta aaaaaagccc cgcagggctt tatatctata tatctgttct
      421 catccaatcc catgtgtctt gggtgagcca tcctgcgtat tctttggtta caccaatcac
      481 aagcaaggtg tcattattgt ccagatgcct acgcaaacga tccgacagtt gttgcgctgt
      541 ttctccggtt gatattagcc atgtggaatc taaagggtgg caccaccccg gcgatttttt
      601 taactcatca tatagtgcat tgtagttttg acctgcttga ttcaggtcat aagagacaca
      661 gtaaactgac atagaaccat cctctttaat caaatgtggg gcagcccgtt agggctgcag
      721 tcacaactta cttcttcttt tcacgattaa ttggaatttt gaacatccca ccgtttggac
      781 gggtgatccg tttgcccttt actgtgatat aagggcgaaa aatgtatgcc tgagactcgt
      841 ctgtctgcat ggtgcatttc ctatttttcg ccacatgagt gttgattttt gctatcaaaa
      901 ttgtactgta gcaaccaaca aatcttgtgg ctggctttat caagctcttg aggtggtact
      961 caagtgccgc atttgtgtat ggtttgttac gggttagttc tggtggtaca ggctaaccca
     1021 cttacttcaa aaaacgtcgt ttttctcctt tcatgcgtca tttcctagat gtttcttcac
     1081 tgtatcagct ccgctgagca taaatgctac aaagagaaac catccccatc ctgaaaccga
     1141 gtgtaaagct agcactgctg agctgacaac tgcggctaaa ggaaccattt ggtatagaaa
     1201 aaatctcata gaccccctgc atttagcttt ttttaacaag tgttccataa cgtgctatct
     1261 attaaacgca cctgttgttt tagaacgctt gtatgctatc cttaagtcct gattttgtca
     1321 atatttactc attttgttgt ttaaggatgg cgtaaggggt gttcttatga tggttaattt
     1381 tagtctttct aacgcatttt cctttatgga tgctcaaaat atgagcatgt tggcggtgtc
     1441 atccgcgaaa gacgacataa acttggctaa cgtggtttca gtgagtgatg ttcggatact
     1501 gaaatctgct ctgatatttg gggctaacgc tagcgggaag tctaatctca tgaaaacaat
     1561 ttctttaatg aaagatattg ttttgcattc cgtgcaagct attgaaagta gcattacaaa
     1621 gaacgtaata ccgtttttgc ttgatgagga tggaccaaat aaaccatctg aaatggaaat
     1681 gacttttatt tcagatgggg ttaaatatag atatggattg tctatttttc aggggaaaat
     1741 aaaagaagaa tggttgtact acacaccaaa atcaagagaa accattctct ttgaaagaga
     1801 tggtctttct attgaggtaa ataaggcggc ttttagtgag tcaaaccttt ttatcaaaga
     1861 agggattatt caaaaaacaa gggaggatgt tccatttgtt tcggttgttg ctggttttaa
     1921 tggtgagcat tcaaaacgag tagttaattg gtttaacaat atcgtattcc tttctggttc
     1981 taatgaaggg atgtttacag caattaccat gaagttaata aatgagtctg aggattttaa
     2041 ggcttggcta aaaaaaatac ttccagcatt ccagattaat gatatttacc tcgatgaggt
     2101 cgcacctgat tttccatttg gaaatataaa aacagatgat gataatttaa aaaatttatt
     2161 tagttcaata caaaattttg caaaaaacca gaaggttttt tctcttagag tagtgaaaaa
     2221 aacagctagc ggtgagattt cagtacctat tgattttgag tctgagggta ctaaaaagat
     2281 agtttatctt cttggtccaa tctacaatgc catgataaac ggtaacttat tgttagttga
     2341 tgagtttgat tctaagtttc acaccctttt gtctaagttt ttgtttagaa tattccatgc
     2401 gcaaagcgag gggcttagcc aaataatagc tgctgttcag gatgttaatc taatgaacac
     2461 tgattgtttt agacgtgacc agatttggtt tgttgataaa gatataaaat caggaagtag
     2521 tcatctctat tctttggttg agtataaaga gaagcagcga actttaaaat catcgtatgg
     2581 gcatgattat ttatctggtg catttgatgc tatcccattg tttgataatt tagatgaatt
     2641 tgaaaaactt atgcctgagg gatgatttat gtcaaagaga ggccgcggtc ttcgtagctc
     2701 ttcgaggaaa gaggaaacaa aatcctcaaa gagcattatg aaaactacag atagatttat
     2761 ttctgttagt tttagatatt ttcataacgt cgatgatatt ggtcagtcta ttgattcttg
     2821 gagggttgac ggcagtttgc cagatttaat tgaaaagctt gtttatctta cagctaatgg
     2881 gataacaaaa gtcagctctg atggtatttt tactaactac actcgatttc cagattcagc
     2941 agttaatgat tttaaatgcc ctgatgaaat atcagttgat gagaactggg gtgttataaa
     3001 aaatatagga ggccagaaaa gaagggttgc tggtttttta aaggataatg tgttttatat
     3061 agtttttttt gataaggatc atcggttttg gaagagcagg aaataggctt agttacgatt
     3121 gtactgtatg ttgaggcaag atcttcttga gatcactgtg ttctcgtcgt aatctctcgc
     3181 tctgtaaacg aaaaaaccgc cttggcgggc ggttttttcg aaggttccaa gagttggcgc
     3241 tcttagaacc aaggtaactg gcttggagga gcgcagtaac caaaacttgt tctttcagtt
     3301 tagccttaac cggcgcataa cttcaagact aactcctcta aatcagttac cagtggctgc
     3361 tgccagtggc gcttttgcat gtctttccgg gttggactca agacgatagt taccggataa
     3421 ggcgcagcag tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc
     3481 ctacccggaa ctgagtgtct ggcgtggaat gagataaacg cggccataac agcggaatgg
     3541 caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccgcct atcagaaatg
     3601 ataggtatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt ccgtttctgt
     3661 gatgcttgtc aggggggcgg agcctatgga aaaacggccg ctgcgcggcc tcctcttttc
     3721 ctcctacctt gatcgctcgg ttttctcgag cttttataag aatggtcttg ccgctcgccg
     3781 cagccgaacg accggagcgt agcgactgag tgagcgagga agcggaaaag agactggttt
     3841 tacactgagc actgacgctc tgaggcttct tatgacgaaa caacagaaaa cagcgctgaa
     3901 catggctaaa ttcatccaga atcagtctct gctcctgctg gagaagctta acgagcttgc
     3961 tctcgatgtc gaggccgacc tttgtgagaa actccacgat gatgcggagc atcttttccg
     4021 tacactgtcc tcgagactgg atgaattaca ggatggtaac tgatggccag agtgtctatt
     4081 agtgaagcgg cacggcttgt taaggttagt cgccccacaa tttacaagat gattaacagt
     4141 gggaagttaa gttacacctc tgttgtaaag cacggtaagg ctattaaagt tattgatact
     4201 tcagagctta ttagagtgtt tggttctctt gatggtgtca tcgatactgt aaaatatgat
     4261 gtaaaaagtg acgcagaatc tacaggtgtt aacagcgttg gtttacatga tttacaacat
     4321 agaatcgctt tacttgaagc tgaaaacgat ggtcttaaag gggctgttaa ggctcgagat
     4381 gagcatattg attcccttcg tcaggctatg cagcttctgg agcacaagca cgaaccttct
     4441 tcaccacccc attccccgtg gtggaaattc tggaaaaaat cctgaccccg tcatgcttcc
     4501 gttttcctct ccggccatcc cggggagttc ctgaaccttc ccaaaaatcg tcagatctct
     4561 gaacgctcct caccactaaa aatacccaca ccccacgcaa aattaacatt ttgcagattt
     4621 gtatttaata tcagtgggtt actttcctga aatttcgttt tttaaatttt atttatatag
     4681 atccgactct atgacgagtc gtcatagagt ccccggcgag tcgtcataga gtcggtaacg
     4741 actcaggact ataaaaacaa taagttaata acgacccgac tctagacacg actccccgtt
     4801 tggttgtttt ttagactcgg cgactctggg tacgactcta gtgaagattc gccggtgaat
     4861 cgt
//
