LOCUS       NZ_CP104462             2150 bp    DNA     linear   CON 04-OCT-2023
DEFINITION  Raoultella ornithinolytica strain MQB_Silv_108 plasmid
            pMQB_Silv108.6.
ACCESSION   NZ_CP104462
VERSION     NZ_CP104462.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30696136
            Assembly: GCF_025349945.1
KEYWORDS    RefSeq.
SOURCE      Raoultella ornithinolytica
  ORGANISM  Raoultella ornithinolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Raoultella.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Quintela-Baluja,M.
  TITLE     Multidrug resistance Raoultella ornithinolytica Strain MQB_Silv_108
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Quintela-Baluja,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2022) Analytical Chemistry, Nutrition and Food
            Science. School of Veterinary Sciences, University of Santiago de
            Compostela, Carballo Calero, s/n, Lugo, Lugo 15704, Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2022
            Assembly Method                   :: Unicycler v. v0.5.0
            Assembly Name                     :: MQB_Silv_108
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 148.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/04/2023 05:03:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,209
            CDSs (total)                      :: 6,085
            Genes (coding)                    :: 5,945
            CDSs (with protein)               :: 5,945
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 44 of 140
            Pseudo Genes (incomplete)         :: 98 of 140
            Pseudo Genes (internal stop)      :: 26 of 140
            Pseudo Genes (multiple problems)  :: 25 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104462.1.
            Bacteria and source DNA available from marcos.quintela@rai.usc.es.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Raoultella ornithinolytica"
                     /mol_type="genomic DNA"
                     /strain="MQB_Silv_108"
                     /isolation_source="wastewater"
                     /db_xref="taxon:54291"
                     /plasmid="pMQB_Silv108.6"
                     /geo_loc_name="Spain"
                     /collection_date="2015"
                     /collected_by="MQB"
     gene            311..916
                     /locus_tag="N2J37_RS30975"
                     /old_locus_tag="N2J37_30975"
     CDS             311..916
                     /locus_tag="N2J37_RS30975"
                     /old_locus_tag="N2J37_30975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_047715645.1"
                     /translation="MQDRSVVNRQGFNPEIDLPYSLRISDFEHAMEDIYDFFHDVNNLL
                     SNKGLHRLDDMMRPAAMSGLISDMITASLAKFSRSLVENKHFNGHPDLVVRGRYPNDSI
                     SSGEEGIEIKTTRKNGGAVDTHGARSQWMCVFVYNVDNETEPASDRKSMKFTEVYLCHV
                     DENDFRRNDRGALGTRTATLHKEGLKKLRESWVYKDLV"
                     /name="hypothetical protein"
     gene            complement(913..2031)
                     /locus_tag="N2J37_RS30980"
                     /old_locus_tag="N2J37_30980"
     CDS             complement(913..2031)
                     /locus_tag="N2J37_RS30980"
                     /old_locus_tag="N2J37_30980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402750.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008170 - N-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006306 - DNA methylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="WP_080917352.1"
                     /translation="MSLAPGVVRDSIIECFLRLGREASIEEITKAVYLKIGKISKSSVR
                     SYLSLNTPGLFERVDRGVYRLNGDAGITPSLDLTSSPKTVGKSTIFFDDAIEWLKRQKD
                     NSIHAVVTDPPYGLVEYTKQQTEKLRAGKGGIWRKPPSFDGHKRAPLPRFTVLREEDLR
                     NISLFFKELSSQVERVVVPGGNIVIASNPLLSHIVADSMSTSGLELRGYIVRQVMTMRG
                     GDRPKNAHDEFGDVSVMPRSQWEPWIVLRKPLDGRVQDNLRKWKTGGFRRISADRPFGD
                     VIKSHPTPAHEKKIAPHPSLKPQNFMRQLVRAVLPLGEGIILDPFMGGGSTLAAANHIG
                     YESIGVEIDREFFDLAEKAIPALSALPSKENN"
                     /name="DNA methyltransferase"
ORIGIN
        1 aaagacatgc aaaagcgcca ctggcagcag ccactggtaa ctgaaaaggt ggatttagat
       61 attgagagtt cttgaagtgg tgacctgact gcggctacac tggaaggaca gtatttggta
      121 tctgcgctcc accaagccag ttacctggtt aagcagttcc ccaactgact taaccttcga
      181 ttaaaccgcc tccccaggcg gttttttcgt ttcaagaggc tacgcccaaa cgacaatgat
      241 cgtcaaggat cttcttggta agatatgaca aagagctttc gaacttttat atcttattca
      301 aggaactaag atgcaagaca ggtcagtagt taatcgacag ggatttaacc cagagatcga
      361 tctgccgtat tcgctaagga ttagtgattt tgagcacgcg atggaagata tttatgactt
      421 cttccatgat gtgaacaact tactatcaaa caaaggtttg cataggcttg atgacatgat
      481 gagaccagca gctatgtctg gcctcatatc agatatgatc acagcttccc tcgctaaatt
      541 ttcaagatcg cttgttgaaa acaagcattt caatggacat ccagacctag ttgttagggg
      601 caggtatccg aatgacagta tctcctcagg agaagaaggc attgaaataa aaaccacccg
      661 gaaaaatggg ggggcagtcg atactcacgg ggccagatct caatggatgt gcgtatttgt
      721 ctacaatgtc gataatgaga ccgaaccggc ctcagacaga aagtcgatga aatttaccga
      781 ggtatatctt tgccacgttg atgaaaatga cttccgtcgt aatgatcgtg gtgcattagg
      841 aaccagaaca gcaacccttc acaaagaagg gttgaagaag ctcagagaaa gctgggttta
      901 caaggactta gtttagttat tttccttgct aggaagagct gatagcgcgg ggatagcctt
      961 ctccgccaaa tcaaagaact ctcggtctat ttcaactcca atgctctcat aacctatatg
     1021 atttgcagct gccaatgtgg agcctccccc cataaatgga tcgagaataa tcccctctcc
     1081 caaaggaagc actgccctca ctaattgtcg catgaaattt tgcggcttta atgagggatg
     1141 tggcgctatc tttttttcgt gtgctggcgt cggatgagat ttgatgacgt cgccaaatgg
     1201 cctatcagct gaaatcctcc tgaatccacc tgttttccac tttcttagat tatcctgaac
     1261 ccttccatct agaggctttc tcaacactat ccatggttcc cattgagaac gaggcatgac
     1321 agaaacatct ccaaattcat catgggcgtt tttgggtcta tccccacctc tcatggtcat
     1381 aacctgacgt acaatgtaac ctcgtagctc taagccagac gtagacatag agtccgcgac
     1441 tatatgggat agtagagggt tggatgcgat aactatattc ccgccaggaa caaccaccct
     1501 ttcgacttgc gaggatagtt ctttgaaaaa taatgatata tttctgaggt cttcttcccg
     1561 caaaaccgta aagcgaggta atggcgctcg cttatgtcca tcaaaggaag gtggctttct
     1621 ccaaatacca cctttccctg cccttagttt ctcagtctgt tgttttgtat actcgactaa
     1681 tccgtaagga ggatcagtta caactgcatg aattgaatta tctttttgtc tttttaacca
     1741 ttcaattgca tcgtcaaaaa aaatcgtcga tttcccaacc gtcttaggtg aagacgtcaa
     1801 atctagggag ggagtaatac cagcatcacc atttaagcga tagacacctc ggtctactcg
     1861 ctcgaacaga cctggggtat ttaaactcag atatgagcga acagaagact ttgaaatttt
     1921 tccaattttt aaataaacag cttttgttat ctcctcaata gaagcttctc ttccaagcct
     1981 taaaaaacat tcaattatcg agtcacgaac aacaccagga gccaaagaca tgtttataaa
     2041 tccagtattt ttatataaaa taataatact aactagacgt caggacgtca atttagttta
     2101 aaagagacgt caaaaaagta tacattaact ttttttttac cataacatcc
//
