LOCUS       NZ_CP104458             8282 bp    DNA     circular CON 04-OCT-2023
DEFINITION  Raoultella ornithinolytica strain MQB_Silv_108 plasmid
            pCol_Silv108.2, complete sequence.
ACCESSION   NZ_CP104458
VERSION     NZ_CP104458.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30696136
            Assembly: GCF_025349945.1
KEYWORDS    RefSeq.
SOURCE      Raoultella ornithinolytica
  ORGANISM  Raoultella ornithinolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Raoultella.
REFERENCE   1  (bases 1 to 8282)
  AUTHORS   Quintela-Baluja,M.
  TITLE     Multidrug resistance Raoultella ornithinolytica Strain MQB_Silv_108
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8282)
  AUTHORS   Quintela-Baluja,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2022) Analytical Chemistry, Nutrition and Food
            Science. School of Veterinary Sciences, University of Santiago de
            Compostela, Carballo Calero, s/n, Lugo, Lugo 15704, Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2022
            Assembly Method                   :: Unicycler v. v0.5.0
            Assembly Name                     :: MQB_Silv_108
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 148.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/04/2023 05:03:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,209
            CDSs (total)                      :: 6,085
            Genes (coding)                    :: 5,945
            CDSs (with protein)               :: 5,945
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 44 of 140
            Pseudo Genes (incomplete)         :: 98 of 140
            Pseudo Genes (internal stop)      :: 26 of 140
            Pseudo Genes (multiple problems)  :: 25 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104458.1.
            Bacteria and source DNA available from marcos.quintela@rai.usc.es.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8282
                     /organism="Raoultella ornithinolytica"
                     /mol_type="genomic DNA"
                     /strain="MQB_Silv_108"
                     /isolation_source="wastewater"
                     /db_xref="taxon:54291"
                     /plasmid="pCol_Silv108.2"
                     /geo_loc_name="Spain"
                     /collection_date="2015"
                     /collected_by="MQB"
     gene            complement(join(7900..8282,1..349))
                     /locus_tag="N2J37_RS30835"
                     /old_locus_tag="N2J37_30835"
     CDS             complement(join(7900..8282,1..349))
                     /locus_tag="N2J37_RS30835"
                     /old_locus_tag="N2J37_30835"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="WP_008321412.1"
                     /translation="MALEQYNVSHAKRQARNAEKTRLVLRWLRDELCSTAETVARLLGI
                     GAVQPVYRFLDSLVAKGLLARAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLDMEARGATGWRTLHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            complement(349..2310)
                     /locus_tag="N2J37_RS30840"
                     /old_locus_tag="N2J37_30840"
     CDS             complement(349..2310)
                     /locus_tag="N2J37_RS30840"
                     /old_locus_tag="N2J37_30840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_044704830.1"
                     /translation="MLNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNDRAAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLVLLGGLVVAVFIESWGETVLRPGYYANADYYLLQPANLFGIAWCAGGLV
                     VGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARQQQVVFLGKDEDGQPVRVPREAWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLAETGDVADHYRISEQKAAKLIAEQFPHGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRAWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QAEAVRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(3768..4100)
                     /locus_tag="N2J37_RS30845"
                     /old_locus_tag="N2J37_30845"
     CDS             complement(3768..4100)
                     /locus_tag="N2J37_RS30845"
                     /old_locus_tag="N2J37_30845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008321410.1"
                     /translation="MEKQYTNELTAEILAGMDQSPFTPEQLAAMSDEARALIEEQEAFC
                     HAHPVTTIYRLAVAGCLTRRGGTGDEFNPNPEEGHKIRLENGLWVSVLTEGCTVTYPDG
                     TQARIL"
                     /name="hypothetical protein"
     gene            4254..4505
                     /locus_tag="N2J37_RS30850"
                     /old_locus_tag="N2J37_30850"
     CDS             4254..4505
                     /locus_tag="N2J37_RS30850"
                     /old_locus_tag="N2J37_30850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008321409.1"
                     /translation="MALVQKKTLNELATNASDDFFGKRFSAGDEVPHSGIYKCTKCNRE
                     VTVNAHSRDNTFPPHYPESPCKSPTWKPYVIADTKAEW"
                     /name="hypothetical protein"
     gene            4518..4772
                     /locus_tag="N2J37_RS30855"
                     /old_locus_tag="N2J37_30855"
     CDS             4518..4772
                     /locus_tag="N2J37_RS30855"
                     /old_locus_tag="N2J37_30855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008321408.1"
                     /translation="MNFKQLIPAQDWYFRHFTDQSDYPVIVYQLAAWALTEEGSITGLV
                     AIYDPKEGKAKLVTPPPVKGNYLHRDQLTDEELAASRKP"
                     /name="hypothetical protein"
     gene            complement(4803..5321)
                     /locus_tag="N2J37_RS30860"
                     /old_locus_tag="N2J37_30860"
     CDS             complement(4803..5321)
                     /locus_tag="N2J37_RS30860"
                     /old_locus_tag="N2J37_30860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044704828.1"
                     /translation="MQKVTDLYPPEIARHKLQNHFAGNTVMLELIQKLNKTSLCTFAAL
                     CDGNVVTTSGYNITADLCVNRASAVAHSLKQKYLPVTARTISTKADVGGAVKQAAFCID
                     EDDLARLKSEPEKMMKECERNLNSQKRTNAQKEMSRLYKEFGEDGILALLRNVARSNGT
                     PPPGAHPAS"
                     /name="hypothetical protein"
     gene            6095..6358
                     /locus_tag="N2J37_RS30865"
                     /old_locus_tag="N2J37_30865"
     CDS             6095..6358
                     /locus_tag="N2J37_RS30865"
                     /old_locus_tag="N2J37_30865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            6348..6647
                     /locus_tag="N2J37_RS30870"
                     /old_locus_tag="N2J37_30870"
     CDS             6348..6647
                     /locus_tag="N2J37_RS30870"
                     /old_locus_tag="N2J37_30870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010848978.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_009365129.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYEVDDPAQKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6709..7086)
                     /locus_tag="N2J37_RS30875"
                     /old_locus_tag="N2J37_30875"
     CDS             complement(6709..7086)
                     /locus_tag="N2J37_RS30875"
                     /old_locus_tag="N2J37_30875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008321415.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQGETHERDGLRLPDETEKTASEALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETVETPAETPRRWWHWGKR"
                     /name="hypothetical protein"
     gene            7156..7335
                     /locus_tag="N2J37_RS30880"
                     /old_locus_tag="N2J37_30880"
     CDS             7156..7335
                     /locus_tag="N2J37_RS30880"
                     /old_locus_tag="N2J37_30880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032934837.1"
                     /translation="MTKQEKTALNMAKFIRDQSLLLLEKLNELDMDDPADMCERLHEDA
                     EKLWLVMRGKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7363..7893)
                     /locus_tag="N2J37_RS30885"
                     /old_locus_tag="N2J37_30885"
     CDS             complement(7363..7893)
                     /locus_tag="N2J37_RS30885"
                     /old_locus_tag="N2J37_30885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044704832.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMAVMAWQTGAYVFRFE
                     DQAQHLAMMKFIGMYHERLNSDDKATYYFTGKNPAWERDKPSGMKLCDYLYRKVEQFAI
                     ENGVQVLDAGKYTPEQIGAMIDVVSERHGESAVNEKLKAELMREYNSRYFNEPVPVLPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     Typing          complement(3305..3484)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.659"
                     /coverage="90.0"
                     /evalue="1.1199999999999999e-60"
     Typing          complement(3..349)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.379"
                     /coverage="99.0"
                     /evalue="6.189999999999998e-54"
     Typing          complement(7904..8280)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.825"
                     /coverage="99.0"
                     /evalue="1.19e-74"
ORIGIN
        1 ccagccggtg cggcagctgc gccgccgaca cccgcgaggg ctgaaacgga ataacgtccg
       61 tcagcggctc gtcctcgtcg aacgagaacg ccacgccgtg cggggtcagc ccccacacgc
      121 tgacctgacg accgtcaacc gggtatttcg cccgggccag cagtcctttc gccaccaggc
      181 tgtccagaaa gcggtaaacc ggctgcaccg ccccgatccc cagcagacgg gctacggttt
      241 cagccgtcga gcagagttcg tcccgcagcc accgcaggac tagccgggtt ttctccgcgt
      301 tccgcgcctg tcgcttcgcg tggctgacgt tgtattgctc cagagccatc agtacagccc
      361 cgcaaaatca tcgtcacccg ggcgctgccg cactgcctca gcctggtact ccgccagcgg
      421 gtccgttttc gccagcaccg ggaaggtttt cagcggaatt tcccggcgat caacccgcag
      481 cgggctgctg aacgccagcc gcgccacgcc cagcccggtc atgaccgcga acccgtccgg
      541 caggtgttgc agggtgttca cgtcgaacag cggccgcgct tccttctgga tgaagctgtc
      601 cccgctgatg tgctccacgt tccccgcctc ccggctggcc cggcggcgct ccacgtccac
      661 ccggatttcc ccggtctgtc cggccgccca ctgcgcggac tcctggctgg ccgcgcgata
      721 gaaccagcgg attggcgtgt tatctagtac cgtggttttc acaaaatcag cgggcagatc
      781 ctgcccgcac tggccgaagt cgcccagcga ctggtgagcc agcagcaggt tacagtcccg
      841 gctgcgcagc gtccccagcg cgttgagtac gtacttcgag agcagatatt tgatttcatc
      901 gagcatcacg ctgacgtgcg gccacgcgcg gaactcgtcc cgcgcaatga tgatttgcgc
      961 gcagcgggca aacagcattt tctgcacccg gatcaccgcc tcgtcgtcca tcgatccgat
     1021 gacgtacaga cagccgccgc cgttgataat ccccgccaca tcaataccgg aatccgtctg
     1081 caggacgctc aggtccgcga cgttctccag cttggtgatc agccccttca cgtccttttt
     1141 cagcgcctcc ggcagcgcat acgccgccgc cagcacctgc tgaatgttcg cgccgtgcgg
     1201 gaactgctcc gcgataagct tcgccgcttt ctgctcgctg atgcggtagt ggtcggccac
     1261 atcgccggtt tccgccaggt taaacccggc aaccagcaga tttttcagtg cgtactggtc
     1321 aaggtcgcgg aacaggttca gctgcgccgg tttccccggc cgcaggtcca gcagggtgaa
     1381 gtccgggcag tgcgcccgaa agacgtgtgg cgcccatgcg tcgcctttcg ggtcgaagta
     1441 caccaccgca tcgccaaaat cgcgcacgca gcggatcagc gcgttggtgg ccatcacgct
     1501 cttgccgctc ccgggtaggc cgaggatctg aatattggtc ttgcgccacg cctcgcgcgg
     1561 aacccgcacc ggctggccgt cctcgtcctt acccaggaac accacctgct gctgccgggc
     1621 ctgctccgtg gaaaaatcgg ccagcgcgtt gaccggcacc ggctccccga aatgcaggtg
     1681 gcgcacgtca gtcatttcgc cggtgcgctg gctgctgcgc cggctctcct gattcagttt
     1741 tgcattgacc gcctcgtcag cggccgcaaa atgaccgtca agcagcagga atgccagtag
     1801 ccgcgccaga agtatcccga cgaccagacc accggcacac caggctatcc cgaacagatt
     1861 agcgggttgc agcaggtaat aatcggcatt cgcgtagtag cccggacgca acaccgtttc
     1921 tccccagctc tcgataaaca cggcgacaac cagccccccc agcagaacca gaccggcagc
     1981 gccggtcagc cagtacgcgt agttccagac atgtcgacga gccctgacca tccgcccggg
     2041 agaatccagc gaggccagca ccgaaaatgg cgcagccctg tcattatcgc gcttatagcg
     2101 atcggcatac tcccgccgct ccttatggct caggcccaca aaacgcatcc gtccggccca
     2161 gagttcagcc agaccgaaaa acaggccgat aaccagaaaa gccccggcca gaccgtaata
     2221 ctcccggtac agccagctga cccacgggta aatctgccgg gccagctccg ggatatgcca
     2281 ctgccaggca agcgaatccg tattcaacat gcaacctctc tcctacagac gacggattta
     2341 ccggcgtgat gcgcagccca tctgcgcaag ctgacgacat gccccaaggc atggcggcgg
     2401 cgttcacgtc cgtggtgaaa gcgttcagcg ctcgccacgg aggccagcct ctttccgccc
     2461 catgcggaaa aggcgcagcc ccggaggaac aacgagcacg cccacagcgt gcgagaggaa
     2521 aaggcggcac cccagtgctg tcttttccgg tgttccggcc cccgtcagag taagcgacag
     2581 cgagcggctg accacccata aaatcaggag cagctttgct gcttcgtcag gtgcgtccag
     2641 caccgtggat ttttgtgggt ggtcaggcgt gagcctgacg ggggccgggt gaatttctgg
     2701 cgtgaccggc gtccagccgg gctaagacag aaagaggggt aaagacgctg cgtcggtggc
     2761 gttcagccag cgtaagcagg ggatttaccc ctgaagtcag tcccgcccta gcggggatga
     2821 cttgcatttc tccgcaagga gaaaaagaaa cagagatagg ttaacgtaaa atgtgtatca
     2881 gaaaaccgac atacttcacc tatcctgatg atttatcgac agggaacaga aaggctttaa
     2941 tcaggagcaa aatgtcacat cagcgcgtcc ggtggccgta ggcaaaaacg ggagatattg
     3001 cgcctcctcg ctcactcggt cgctacgctc cggccgtgaa actgcggcgg gcgctgcgga
     3061 ctcacgagca gggcaaacag gcatagagag aaaataatac gaagctgcgc cggtggcgtt
     3121 tttccatagg ctccgccctc ctgccagagt tcacataaac agacgctttt taggtgcatc
     3181 tgtgggagcc gtgaggctca accatgaatc tggcagtacg ggcgaaaccc gacaggactt
     3241 aaagatcccc acagtttccg gtgggtcgct ccctcttgcg ctctcctgtt ccgaccctgc
     3301 cgtttaccgg atacctgttc cgcctttctc ccttgcggga agtgtggcgc tttctcatag
     3361 ctcacacact ggtatctcgg ctcggtgtag gtcgttcgct ccaagctggg ctgtaagcaa
     3421 gaactccccg ttcagcccga ctgctgcgcc ttatccggta actgttcact tgagtccaac
     3481 ccggaaaagc acggtaaaac gccactggca gcagccactg gtaactggag ttcgcaggaa
     3541 cgtaagagtg aaaaagtaac tttttcgctt gaagaagcca gtttgaagca gctaaactga
     3601 ctgtactgtt ttggtgactg ttcctgcgaa gatcagttac cgcccggttt cggcgagttt
     3661 gccgctcgac gaacccgaac taacccgcct tacggcgggt tttttcgttt acagagcaag
     3721 agattacgcg cagtggtaaa cgatctcaag tggatctttt taagatccta cagaatccgg
     3781 gcttgcgtgc catcaggata cgtcacggta caaccttcag tcagcacgct gacccacaac
     3841 ccgttctcca gccggatttt atggccttcc tcagggttcg gattgaactc atcgccggta
     3901 ccaccgcgcc gggtcagaca gcccgccacc gccagtcggt aaatggtcgt caccggatgc
     3961 gcatggcaga atgcctcctg ctcctcaatc aacgcgcgtg cttcatcgct catcgcagcc
     4021 agttgttcag gagtaaaagg tgattgatcc attccggcca gaatctccgc ggtcagttca
     4081 ttggtgtact gcttctccat actaatttct tgtactttaa atggttaaaa tcacgccatc
     4141 atactcataa tcctgactta accaaaactc agccgcttga aaaaacgcca caaagaccac
     4201 atttataaaa tgggtaagaa tgcccgaaaa atatcgatgg gaaggaaagc agaatggcct
     4261 tagtacagaa aaaaacgctg aatgaactgg caacaaacgc cagcgatgac ttcttcggta
     4321 agcgattttc agccggagat gaagttccac actcggggat ctacaaatgc acgaaatgta
     4381 atcgtgaagt gacggtaaat gcccactcca gagacaatac atttccgcca cattacccgg
     4441 aaagcccgtg taaatcaccg acgtggaaac cctatgtcat tgcagacaca aaagcggaat
     4501 ggtgaaggag ctaatacatg aactttaagc aactcatccc cgcacaggac tggtattttc
     4561 gtcattttac cgaccagtct gattatccgg tgatcgttta tcagcttgct gcatgggcac
     4621 tgactgagga aggaagcatt accggccttg tggcgatata cgatccgaaa gaaggcaaag
     4681 caaaactggt gacaccaccg ccggtaaaag ggaactatct gcaccgagat cagctgacag
     4741 atgaagagct ggcggcatcc agaaaacctt aaaaataaag cccctgaggg gctttactgg
     4801 tatcagcttg cagggtgtgc gcctggcggc ggtgtaccgt tcgaccgggc aacattgcgt
     4861 aacagcgcca gtatgccgtc ttcaccaaac tccttgtaca accgactcat ttccttctga
     4921 gcattggttc gcttctggct gttcaggtta cgttcgcact ctttcatcat cttttccggc
     4981 tctgatttca gccttgcaag atcgtcttca tcgatacaga aagccgcctg ctttaccgca
     5041 ccacctacat cggccttagt cgaaatcgtt ctggccgtaa ccggcagata tttttgctta
     5101 agcgaatggg ccacggctga cgcacggtta acacacagat cggccgtaat gttatagccg
     5161 gaagtggtca cgacgttgcc gtcacagagc gccgcaaaag tacacaggct ggttttattt
     5221 agtttctgga ttaactccag cataacggta ttaccggcga agtgattctg gagcttatgg
     5281 cgagcaattt caggaggata cagatcggtt actttttgca tttatagcac ctgtttacag
     5341 aggataaata acaggtgacg gtgtccggat gagccttcgc acatcaaaaa actgtgaaaa
     5401 atgcctggca agtcggtacc aagttactac ataatgcagc accacttgac aaacgcccta
     5461 atcgggcgtt tgttgatcag gtaagattca gttttcgcga actgagtcac cggcagctat
     5521 cagaatcctt agtttctcag gccgaagatc tgatagctgc ctcgtttttc actgacaacc
     5581 tcacgatcat cagaatccct tattctaccc tattccataa gcgcacacat agcttttttg
     5641 ctgatctttc gcgcttgatt tacaaggaaa ttacctgtcg ggtagatgaa attagcaaca
     5701 gcgctatcag atgacgctaa tgttaaactc atgtttaaac atacagcgaa aagttgacgc
     5761 aaaaatcgtc agctggatgc ctcacaatcc cgtcatttac cataacatcc gttgcccgca
     5821 ccccctgaga gcctctgagg gcatttctga cgggaaaccg ataaaacaga caacaaacca
     5881 cccgcaacac ccccggaaac gcgccggata aattgtacct gaacgcatgg ctatgcacgt
     5941 aactgcatgg catgggattt gacgtaaaat ggatgccgta agcccttttt taccgacttt
     6001 ctgcttcatc agcctgactg acacgagcat attctttcag gccgcctcct gctaaacttt
     6061 ttcatattgc cgcattaacc cgggaggctc cgcaatgagc aacacgacac actacgaaaa
     6121 cgccaacttt cttcgggaac tggctgaaag cctgccccgc atactgccgg aaggtggccc
     6181 ggacaaagct gcgctgttgc agcgcctggc taacgaagaa ctggcccagg cagagtatga
     6241 agatcaggtt cgcgccaaag tgaccgccgc ccgtgctgac acgcgtccgg gcatgaccac
     6301 ggaacaactc cgccagcgcc tgcatggccg ttatcaggaa ctgcgcgatg ccgtatgacg
     6361 tggaatggaa gcagggagcc atcgaagatg taacgacact gtttgactat atcgcggaaa
     6421 attcatcgct gtgggatgcg cagaacgtta ccgaccgggt tctcgctgct gcggacaaac
     6481 tggcggactt cccgcgcctc tacgaagtgg actcacgtta tggcgaagac gttcgccgga
     6541 tcagcctgat gggacaacat gtcctctatg aagtggacga ccccgcgcag aaagtccggg
     6601 ttctggccgt ggtcggccag cgccagaatc cccataccgt tcgctaataa ccggtgacag
     6661 gggcgcaatt cattgcgtgc ccgtcgccgg ttggacaagc gcagcgcgtc agcgttttcc
     6721 ccaatgccac caccggcgcg gcgtttcggc cggtgtctct acggtctccg gtttgtgttc
     6781 cagcagtctc atggcctgca tttcctgccg caacccggcc acctcctccc tgagtgcttt
     6841 tatctccgcg atcaacgctt cagaggccgt tttttcagtc tcatctggca gtctcaaccc
     6901 gtcccgctcg tgagtctcac cctgtcttaa ctcaccgtac acccggataa gctcgctggt
     6961 atccacaacc cgaccgccat cagcctccgt ccggtatgat acgcggcctt tcgccatatc
     7021 ccggtacagg gaactccggg ctttccccgt cagttccgtc gcctgtctca ccgtcaccca
     7081 cgccattgtc tcaccccgct atttttgaga cagtataccc gactggcgca caccatgccc
     7141 ggaagggaaa aaatcatgac gaagcaggaa aaaaccgccc tcaacatggc aaaattcatc
     7201 agggatcagt cgctgctgtt gctggaaaag ctgaacgagc tggacatgga cgacccggcc
     7261 gacatgtgcg aacgacttca cgaggatgcg gaaaaactct ggctggtaat gcggggtaag
     7321 ctcggggaag aataacagca ccggcatggc cggtgctggc gtttattttt tgccgaacgg
     7381 ccaccaccgg cgggcgggtt tatcctccgg caatacgggc accggctcgt taaaataccg
     7441 gctgttatac tctcgcatca gctccgcttt cagcttctcg ttcaccgcac tctcaccgtg
     7501 ccgctcactc accacgtcaa tcatcgcgcc gatctgctcc ggggtgtact tccctgcgtc
     7561 cagtacctgc acgccgttct cgatggcgaa ctgctcaacc ttgcggtaaa gataatcaca
     7621 cagcttcatt ccggacggtt tatcacgttc ccacgccgga tttttgccgg tgaagtaata
     7681 cgtggctttg tcatcgctgt tcagccgttc gtgatacatg ccgataaact tcatcatcgc
     7741 cagatgctgg gcctgatctt caaaccggaa tacatatgcc ccggtctgcc aggccatgac
     7801 cgccatgtct tccagatgct cgctggttgg cataatgcag cgctcgccgt ctttgtcttc
     7861 cacatcctca ataaaaacca tgtacagcgc cataatccct cactctgttg tcataaattt
     7921 aaagcacgcg aacagcctct cccggtccag ttccgccgtt ttgcgggctt cgccggtctg
     7981 cgggttcacg atttcatcca ccgccagaat cgcccgctgt acccggacaa gcgtcggccg
     8041 gtcggggcag atgtaccaga tttcatcgat gccgttcgcc ttacggttga acagataccc
     8101 ggacaccact tcctgatacc ggcgacggct cttgacggtg cgctccacct caaacgccac
     8161 cgttttaccg tccagctctg ccagcgcatc cggcaccttc atacctttaa gactcaggcg
     8221 gtgcagggtg cgccagccgg tggcccctct ggcttccata tcaagacgca gggactgcac
     8281 gg
//
