LOCUS       NZ_CP104457             9838 bp    DNA     circular CON 04-OCT-2023
DEFINITION  Raoultella ornithinolytica strain MQB_Silv_108 plasmid
            pCol_Silv108.1, complete sequence.
ACCESSION   NZ_CP104457
VERSION     NZ_CP104457.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30696136
            Assembly: GCF_025349945.1
KEYWORDS    RefSeq.
SOURCE      Raoultella ornithinolytica
  ORGANISM  Raoultella ornithinolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Raoultella.
REFERENCE   1  (bases 1 to 9838)
  AUTHORS   Quintela-Baluja,M.
  TITLE     Multidrug resistance Raoultella ornithinolytica Strain MQB_Silv_108
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9838)
  AUTHORS   Quintela-Baluja,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2022) Analytical Chemistry, Nutrition and Food
            Science. School of Veterinary Sciences, University of Santiago de
            Compostela, Carballo Calero, s/n, Lugo, Lugo 15704, Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2022
            Assembly Method                   :: Unicycler v. v0.5.0
            Assembly Name                     :: MQB_Silv_108
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 148.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/04/2023 05:03:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,209
            CDSs (total)                      :: 6,085
            Genes (coding)                    :: 5,945
            CDSs (with protein)               :: 5,945
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 44 of 140
            Pseudo Genes (incomplete)         :: 98 of 140
            Pseudo Genes (internal stop)      :: 26 of 140
            Pseudo Genes (multiple problems)  :: 25 of 140
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104457.1.
            Bacteria and source DNA available from marcos.quintela@rai.usc.es.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9838
                     /organism="Raoultella ornithinolytica"
                     /mol_type="genomic DNA"
                     /strain="MQB_Silv_108"
                     /isolation_source="wastewater"
                     /db_xref="taxon:54291"
                     /plasmid="pCol_Silv108.1"
                     /geo_loc_name="Spain"
                     /collection_date="2015"
                     /collected_by="MQB"
     gene            join(9834..9838,1..157)
                     /locus_tag="N2J37_RS30765"
                     /old_locus_tag="N2J37_30765"
     CDS             join(9834..9838,1..157)
                     /locus_tag="N2J37_RS30765"
                     /old_locus_tag="N2J37_30765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049165871.1"
                     /translation="MHEPPVQSDCCVLSGNYHLESNPERHDKAPLAAATGNGFRGVGLE
                     VMRLLRLN"
                     /name="hypothetical protein"
     gene            complement(315..431)
                     /locus_tag="N2J37_RS30770"
                     /old_locus_tag="N2J37_30770"
     CDS             complement(315..431)
                     /locus_tag="N2J37_RS30770"
                     /old_locus_tag="N2J37_30770"
                     /inference="COORDINATES: protein motif:HMM:NF014457.3"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_072072242.1"
                     /translation="MVLAACQVNNVRDTGGGAVSPSSTVTGVTLDSGTNGNP"
                     /name="colicin release lysis protein"
     gene            complement(464..1732)
                     /locus_tag="N2J37_RS30775"
                     /old_locus_tag="N2J37_30775"
     CDS             complement(464..1732)
                     /locus_tag="N2J37_RS30775"
                     /old_locus_tag="N2J37_30775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011191342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISApu1 family transposase"
                     /protein_id="WP_260990891.1"
                     /translation="MTIINANYYLEQLLAPAALERIARLCKFCLRQRAITPAMLVPALL
                     RAMGGDQVNDIAGLHRHFNALQLVNTHQVSYKPFHNQLRKESFALFMKALVERAIALRI
                     DHQLKAASLGAFKQVLLHDGTSFAVHRRLAADFPGRFKTISPAAIECHMTMSLSEQSPL
                     CMSVSADTAGERQFLPEAHTLTNCLLLADAGYIDRAWFEQVNDAGGFYLVRGTKSLNPK
                     IIQAWRGDGREVPKLAGLSLREAGRRRCRAEVLDMVVKSGQVEYRLIRRWFAEEKRFCL
                     WMTNLPRAAWSAEQVMSLYRCRWQVELLFKEWKSHNRLKGFVTGEKAIAEGLVWTSLLS
                     LVMKRRVAQSVMSGALSMLKASKNSATWWLPLLEAVAHRALTEIRERLEWAADYLAKNA
                     CRTKQRKSIQNSTLEGVLNGLAA"
                     /name="IS4-like element ISApu1 family transposase"
     gene            complement(2050..2421)
                     /locus_tag="N2J37_RS30780"
                     /old_locus_tag="N2J37_30780"
     CDS             complement(2050..2421)
                     /locus_tag="N2J37_RS30780"
                     /old_locus_tag="N2J37_30780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019838990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004099020.1"
                     /translation="MTQERIQTRYPLGGPFATQAELEQAITQAITTHRQDFFPLLERLV
                     DVGDTRVNLSQENPLRVLSIELADGREGVAHINYESNFAESCLIINEYDQHQTSLAFRL
                     DGDALIFDVELPIAWNFNN"
                     /name="hypothetical protein"
     gene            2578..>3507
                     /locus_tag="N2J37_RS30785"
                     /old_locus_tag="N2J37_30785"
                     /pseudo=""
     CDS             2578..>3507
                     /locus_tag="N2J37_RS30785"
                     /old_locus_tag="N2J37_30785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011222061.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISApu2 family transposase"
                     /name="IS4-like element ISApu2 family transposase"
     gene            complement(3752..4792)
                     /locus_tag="N2J37_RS30790"
                     /old_locus_tag="N2J37_30790"
     CDS             complement(3752..4792)
                     /locus_tag="N2J37_RS30790"
                     /old_locus_tag="N2J37_30790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="WP_064483933.1"
                     /translation="MNRYNRNDKPDWVPPRSIKLLDQVRERVRYLHYSLQTEKAYVYWA
                     KAFVLWTARSHGGFRHPREMGQAEVEGFLTMLATEKQVAPATHRQALNALLFLYRQVLG
                     MELPWMQQIGRPPERKRIPVVLTVQEVQTLLSHMAGTEALLAALLYGSGLRLREALGLR
                     VKDVDFDRHAIIVRSGKGDKDRVVMLPRALVPRLRAQLIQVRAVWGQDRATGRGGVYLP
                     HALERKYPRAGESWAWFWVFPSAKLSVDPQTGVERRHHLFEERLNRQLKKAVVQAGIAK
                     HVSVHTLRHSFATHLLQAGTDIRTVQELLGHSDVSTTMIYTHVLKVAAGGTSSPLDALA
                     LHLSPG"
                     /name="integron integrase"
     gene            4928..5728
                     /locus_tag="N2J37_RS30795"
                     /old_locus_tag="N2J37_30795"
     CDS             4928..5728
                     /locus_tag="N2J37_RS30795"
                     /old_locus_tag="N2J37_30795"
                     /EC_number="3.5.2.6"
                     /function="hydrolysis of beta-lactams"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-10 family class D beta-lactamase OXA-101"
                     /protein_id="WP_063860937.1"
                     /translation="MKTFAAYVITACLSSTALASSITENTFWNKEFSAEAVNGVFVLCK
                     SSSKSCATNNLARASKEYLPASTFKIPNAIIGLETGVIKNEHQVFKWDGKPRAMKQWER
                     DLSLRGAIQVSAVPVFQQIAREVGEVRMQKYLKKFSYGNQNISGGIDKFWLEGQLRISA
                     VNQVEFLESLFLNKLSASKENQLIVKEALVTEAAPEYLVHSKTGFSGVGTESNPGVAWW
                     VGWVEKGTEVYFFAFNMDIDNENKLPLRKSIPTKIMASEGIIGG"
                     /name="OXA-10 family class D beta-lactamase OXA-101"
     gene            5733..6287
                     /locus_tag="N2J37_RS30800"
                     /old_locus_tag="N2J37_30800"
     CDS             5733..6287
                     /locus_tag="N2J37_RS30800"
                     /old_locus_tag="N2J37_30800"
                     /EC_number="2.3.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003159191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ib3"
                     /protein_id="WP_032488579.1"
                     /translation="MTNSNDSVTLRLMTEHDLAMLYEWLNRSHIVEWWGGEEARPTLAD
                     VQEQYLPSVLAQESVTPYIAMLNGEPIGYAQSYVALGSGDGWWEEETDPGVRGIDQLLA
                     NASQLGKGLGTKLVRALVELLFNDPEVTKIQTDPSPSNLRAIRCYEKAGFERQGTVTTP
                     DGPAVYMVQTRQAFERTRSDA"
                     /name="aminoglycoside N-acetyltransferase AAC(6')-Ib3"
     gene            <6582..6929
                     /locus_tag="N2J37_RS30805"
                     /old_locus_tag="N2J37_30805"
                     /pseudo=""
     CDS             <6582..6929
                     /locus_tag="N2J37_RS30805"
                     /old_locus_tag="N2J37_30805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011222061.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /name="IS4 family transposase"
     gene            complement(7080..7337)
                     /locus_tag="N2J37_RS30810"
                     /old_locus_tag="N2J37_30810"
     CDS             complement(7080..7337)
                     /locus_tag="N2J37_RS30810"
                     /old_locus_tag="N2J37_30810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_025714833.1"
                     /translation="MAGNKISDFTEEEFTAFVSKIIAADFPTDKENDDAIYSFAQLTEH
                     PAGWNLIFKPKVGEDSSAKGIVKTVKEWRAANGKPGFKKA"
                     /name="bacteriocin immunity protein"
     gene            complement(7339..>7821)
                     /locus_tag="N2J37_RS30815"
                     /old_locus_tag="N2J37_30815"
                     /pseudo=""
     CDS             complement(7339..>7821)
                     /locus_tag="N2J37_RS30815"
                     /old_locus_tag="N2J37_30815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /name="S-type pyocin domain-containing protein"
     gene            <7820..8068
                     /locus_tag="N2J37_RS30820"
                     /old_locus_tag="N2J37_30820"
                     /pseudo=""
     CDS             <7820..8068
                     /locus_tag="N2J37_RS30820"
                     /old_locus_tag="N2J37_30820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178052.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(8816..9238)
                     /locus_tag="N2J37_RS30825"
                     /old_locus_tag="N2J37_30825"
     CDS             complement(8816..9238)
                     /locus_tag="N2J37_RS30825"
                     /old_locus_tag="N2J37_30825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_025714834.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTSINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKREPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(9238..9429)
                     /locus_tag="N2J37_RS30830"
                     /old_locus_tag="N2J37_30830"
     CDS             complement(9238..9429)
                     /locus_tag="N2J37_RS30830"
                     /old_locus_tag="N2J37_30830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     ARG             5734..6284
                     /name="aac(6')-Ib3"
                     /gene="aac(6')-Ib3"
                     /gene_name="aminoglycoside N-acetyltransferase AAC(6')-Ib3"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             4929..5725
                     /name="blaOXA-101"
                     /gene="blaOXA-101"
                     /gene_name="OXA-10 family class D beta-lactamase OXA-101"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             5722..6287
                     /name="AAC(6')-Ib10"
                     /gene="AAC(6')-Ib10"
                     /gene_name="AAC(6')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.46"
                     /coverage="92.61"
     Typing          2..106
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.34"
                     /coverage="93.0"
                     /evalue="3.4799999999999997e-38"
     Typing          9478..9838
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.441"
                     /coverage="92.0"
                     /evalue="1.06e-119"
ORIGIN
        1 cgaacccccc gttcagtccg actgctgcgt cttatccggt aactatcatc ttgagtccaa
       61 cccggaaaga cacgacaaag cgccactggc agcagccact ggtaacggat ttagaggagt
      121 tggtcttgaa gtcatgcgcc tgttaaggct aaactgaaag aacaagtttt ggtgactgcg
      181 ctcctccaag ccagttacct tggttcaatc acctgcaagt gtttgaacct tcggaaaaac
      241 cgccctacaa ggcggttttt tcgtttacag agcaagagat tacgacgatc caaatgggat
      301 ctcaagaaga tcctttacgg attgccgttc gtaccgctat caagcgtgac tccggtcacc
      361 gtcgatgaag gtgaaacagc cccacctccg gtgtctcgaa cattattgac ctgacatgct
      421 gccagaacca cagaaacggt aacaacccct catgtacagg accttaagcg gcgagtccat
      481 ttaaaacgcc ttccaaggta ctgttctgta tcgactttct ctgcttggtt cggcaggcgt
      541 tcttggccaa ataatcggca gcccattcca atctttccct tatttctgtc agcgccctat
      601 gggccactgc ttccaatagc ggtagccacc acgtcgcact gttcttcgac gctttcagca
      661 tcgatagcgc cccactcatc acactctgcg cgacccgtct cttcatcacc agcgacagca
      721 ggctcgtcca caccaacccc tcggcaatcg ctttctcgcc cgtcacaaac cccttgagcc
      781 ggttgtgcga tttccactct ttaaataaca actccacctg ccaacggcag cgatacaggc
      841 tcatcacctg ctctgctgac catgctgctc gcggtaggtt cgtcatccac agacagaacc
      901 gcttttcctc agcaaaccag cgccggatca accggtactc gacctggccg gacttcacca
      961 ccatatccag tacctcggcc cgacagcgtc gccgtccggc ctctctcaga gatagtcccg
     1021 ccagcttggg tacttcccgt ccatcaccgc gccaggcttg gattatcttg gggttcaggc
     1081 ttttcgtgcc gcgcaccaga taaaatccgc cagcgtcgtt cacctgctca aaccaagccc
     1141 tgtcaatgta acctgcgtca gccagcagca ggcagttggt cagcgtgtgg gcttcaggca
     1201 gaaattgccg ttcccctgcg gtatcggcag acacactcat gcacaacggg ctctgctccg
     1261 acagtgacat ggtcatatgg cactcgatag ctgccggact gatggtcttg aaccggccgg
     1321 gaaaatcagc agcaaggcgc cgatgcaccg caaaggaggt gccatcgtgc agtaacacct
     1381 gcttgaacgc gccgagggag gctgctttga gttgatggtc gatacgcaag gcgatggccc
     1441 gctcgaccag ggctttcata aacagggcaa agctctcttt gcgcagctga ttgtgaaacg
     1501 gtttgtaaga aacctgatgg gtgtttacca gttgcaaggc attgaaatgg cggtgtaaac
     1561 cggcaatgtc attgacctgg tcgcctccca tggcccgcag cagtgcaggt acgagcatgg
     1621 ctggggtaat ggcacgctga cgcagacaaa acttgcagag ccgagcaatg cgctcgagtg
     1681 cggcgggagc caagagttgc tccagatagt aattagcgtt gataatagtc ataggcttcg
     1741 cgcgtgttga ttttgtttgg cgataaaatc agatcacaaa cgaagccttt tttatatcta
     1801 tttcaattgg ttgccgctaa agatcctgta catgagtgag ggttatggtg gctatatgct
     1861 ggatattgct attcagatga cagccatatg gggaagatta caggcgcctg aaataacagc
     1921 agagtatgga gtgactacca cggctggagg ttggcacggg ctgatcccct ctccctttgg
     1981 gagagggtta gggtgagggg agctggctga ccgggtgtag caaccaggcg gcattacacc
     2041 gctgaatctt cagttattga aattccacgc gatcggcagt tcgacgtcga agataagtgc
     2101 atccccgtcg aggcgaaagg ccaggctggt ctggtgctgg tcgtattcgt tgataatcag
     2161 gcagctctcg gcgaagttgg actcgtagtt gatatgggcc actccttcac ggccgtcagc
     2221 gagttcgatg ctgagcaccc gcagcgggtt ctcctgactg aggttgaccc gggtgtcccc
     2281 cacgtccacc agccgttcca gcagagggaa gaaatcttgt ctgtgggtcg ttatggcctg
     2341 ggtgatggcc tgctccagct cagcctgggt ggcgaagggg ccgccgagtg ggtagcgggt
     2401 ctggatcctt tcctgagtca ttgcgtttcc tcctgaactg agatgtggca acagtttggc
     2461 cgctgcagtg acctgtcatg tacaggatct ttagctacaa ccaattgaaa tagatagaaa
     2521 aaaggcttcg tttgtgatct gatttaatcg caaaaaaaaa ccgacacgcc cgaagccatg
     2581 actattatca atgcaaccca gtacctcaag caacttctta gctcttctga actcaaccgg
     2641 atcggtaaat ttaccggttt ttgtcagcga ctccgggata tccagcctgc gagactactc
     2701 ccggctctgc tctcagggct tggttgcgac aaggtggatg gtattgctgg tttgcatcgc
     2761 catttcaatg cgttgcagct ccatgatact gaccaaatag cgtataaacc ctttcacaac
     2821 cagttacgca agcagggttt tcccttgttc atgcgcgcac tggttgaacg agccatcgcc
     2881 ttacgcctca aggagtgtct accggacgcc cacggactgg caggctttga gcaggtactg
     2941 ctgcaggacg ggtcgtcatt cgcgttacat ccgcaattgg cagaacattt cccgggccga
     3001 ttcaataagc acagccccgc tgcggtagag tgtcatatga ccatgtcctt gctggaccag
     3061 tcgccgatat cgatgagtat cacggcggat accgaatcgg agcgcaagca tcttccggcc
     3121 gcaaattcgt taaataacaa actgttattg gcggatgccg gttacatcag ccgcgagtac
     3181 tttgctgatg tgaccaaagc caaaggaagc tatctggtca gagggagtca aaatctcaat
     3241 cccaaagtac aggcagctta ccgacaggac ggcagggaaa tgcccaagtt actcggaaaa
     3301 aagctcaagg atatcgatcg gagaagctgc agagccgagg ttctggatct ggatgtgagt
     3361 tcgggaaagt ataaatatcg actcataaga cgttggtttg cagaggaaaa acggttctgt
     3421 atatggatga ccaatcttcc gcgtgagcga tggccagccg agaaggtgat ggcattgtat
     3481 cgttgccgtt ggcagatcga actgttgttg ggggaaccgc agaattcgga aaaaatcgta
     3541 cgctaagcta acggtgttct cgtgacagct ctttgactag gctttctaag gccattctgg
     3601 ggtcgtctca gaaaacggga aaaatagcac gctaaggcgc gcgacggctt ttacctagca
     3661 gcagtgcaag tctgcacctg cgaggcatga catgcaggag ggcgctatcg accctgagcc
     3721 gccatccaga tgaaagcacc acaaccgtca ttcagccggg cgacaagtgc aaggccaagg
     3781 cgtccagcgg gctggaggtg cctccggcag cgactttcag cacatgcgtg tagatcatcg
     3841 tcgtgctcac gtccgaatgc cccaacaact cttgcaccgt tcggatgtct gtgcctgctt
     3901 gcagcaagtg ggtggcgaat gagtggcgca gggtgtggac agatacgtgt ttggcaatgc
     3961 cagcctgaac taccgctttt tttagttgcc ggttcagtct ttcctcaaac aagtggtggc
     4021 ggcgctcaac gccggtttgt gggtccacag acagcttggc cgatggaaac acccagaacc
     4081 aggcccagct ctcgcccgcc ctggggtact tgcgctccag tgcatgaggc agatacacgc
     4141 ctccgcgccc cgtggcacgg tcctgccccc acacagcgcg gacctgaatc agctgcgccc
     4201 gcaaccgagg tacgagcgcc ctgggcagca tcaccacgcg gtccttgtcg cccttgccgc
     4261 tgcgcacaat gatcgcgtgg cggtcgaaat ccacatcctt gacccgcagg cccagcgctt
     4321 cgcgcaggcg caacccactg ccgtaaagca gggcggccaa cagcgcttcg gtgcccgcca
     4381 tgtgcgaaag caacgtctga acctcctgca ccgtcagcac caccggaatc cgcttgcgtt
     4441 ctggcggccg accaatctgc tgcatccacg gcaattccat gcccagcacc tgccgataca
     4501 agaacaacag cgcgttgagc gcctgccggt gggtggccgg cgccacttgc ttctcggtgg
     4561 cgagcatggt cagaaaaccc tcgacttcag cttgccccat ttcgcgcgga tgtcgaaacc
     4621 caccatggct gcgggccgtc cacaacacaa atgccttggc ccagtagaca taagctttct
     4681 cggtctgtag gctgtaatgc aggtagcgaa cccgttcgcg cacctgatcg agcagcttga
     4741 tggatcgggg agggacccag tcaggtttgt catttctgtt atacctgttc atgcatccag
     4801 tatgatgctt tacagaattg atttcaaaca ctttttttgg gtgccgtgcg acattgttta
     4861 acgaccacgg ttgtgggtat ccggtgtttg gtcagataaa ccataagtta gccaccaaga
     4921 aggtgccatg aaaacatttg ccgcatatgt aattactgcg tgtctttcaa gtacggcatt
     4981 agctagttca attacagaaa atacgttttg gaacaaagag ttctctgccg aagccgtcaa
     5041 tggtgttttc gtgctttgta aaagtagcag taaatcctgc gctaccaata acttagctcg
     5101 tgcatcaaag gaatatcttc cagcatcaac atttaagatc cccaacgcaa ttatcggcct
     5161 agaaactggt gtcataaaga atgagcatca ggttttcaaa tgggacggaa agccaagagc
     5221 catgaaacaa tgggaaagag acttgagctt aagaggggca atacaagttt cagcggttcc
     5281 cgtatttcaa caaatcgcca gagaagttgg cgaagtaaga atgcagaaat atcttaaaaa
     5341 attttcatat ggtaaccaga atatcagtgg tggcattgac aaattctggt tggagggtca
     5401 gcttagaatt tccgcagtta atcaagtgga gtttctagag tctctatttt taaataaatt
     5461 gtcagcatca aaagaaaatc agctaatagt aaaagaggct ttggtaacgg aggctgcgcc
     5521 tgaatatctt gtgcattcaa aaactggttt ttctggtgtg ggaactgagt caaatcctgg
     5581 tgtcgcatgg tgggttggtt gggttgagaa gggaacagag gtttactttt tcgcctttaa
     5641 catggatata gacaacgaaa ataagttgcc gctaagaaaa tccattccca ccaaaatcat
     5701 ggcaagtgag ggcatcattg gtggctaaca tcgtgaccaa cagcaacgat tccgtcacac
     5761 tgcgcctcat gactgagcat gaccttgcga tgctctatga gtggctaaat cgatctcata
     5821 tcgtcgagtg gtggggcgga gaagaagcac gcccgacact tgctgacgta caggaacagt
     5881 acttgccaag cgttttagcg caagagtccg tcactccata cattgcaatg ctgaatggag
     5941 agccgattgg gtatgcccag tcgtacgttg ctcttggaag cggggacgga tggtgggaag
     6001 aagaaaccga tccaggagta cgcggaatag accagttact ggcgaatgca tcacaactgg
     6061 gcaaaggctt gggaaccaag ctggttcgag ctctggttga gttgctgttc aatgatcccg
     6121 aggtcaccaa gatccaaacg gacccgtcgc cgagcaactt gcgagcgatc cgatgctacg
     6181 agaaagcggg gtttgagagg caaggtaccg taaccacccc agatggtcca gccgtgtaca
     6241 tggttcaaac acgccaggca ttcgagcgaa cacgcagtga tgcctaaccc ttccatcgag
     6301 ggggacgtcc aagggctggc gcccttggcc gcccctcatg tcaaacgttg agcgactgct
     6361 tacccgtttg acggtcggcc gctcctggcc gggagccgaa acccagagtt ggggcgtcaa
     6421 cctgatctat acctcaactg ggcgtcagtc ggctcactgg catcgcgtca gactagggcc
     6481 gctttctgca ttatctgacg cctaaacgtc aaactctatg gcctcaacct gacaggccgc
     6541 atgcttagcg tacgattttg tccgttttct ctaccgtccc cttgttcaaa gagctgaaat
     6601 cacataaccg gttgaaggcg ttcgcgacac gacaaaaagc gatagcggaa ggcttgatct
     6661 gggctagttt gctggccttg ctggtcaaac gtcggctggc gctgacgctg attggcagcg
     6721 cggagttatc gatgctgaag attgccaaaa acagcgcgct ttggttaatg ccgatattgg
     6781 cgagcatcat ccatggtgcg tggcaggaaa taacagagaa gttggagtgg gcaatggtgt
     6841 atttatccaa aaacggcaaa aagagtaggc agagaaaatc caagcaaaac aacacgttag
     6901 acggtattat taattcgctt gccgcttaag gttctgtaca tgaacaaccc ctaggaaaag
     6961 taattttttc atatatcacc ttttattttt attattctag gcaaatggat atctagtaat
     7021 tattcttcaa atccagagtt acaaatgagc gggctcataa cttgaacccg caaattcaat
     7081 cacgcttttt taaaacctgg cttaccgtta gcagctcgcc attcctttac ggttttaaca
     7141 attcctttcg ccgaagaatc ttcgcctacc ttaggcttga agatgaggtt ccaaccagca
     7201 ggatgctctg ttagttgggc gaacgaataa atagcatcat cgttctcttt gtcagttgga
     7261 aaatcagcag cgattatttt actaacaaaa gcagtaaatt cttcttccgt aaaatctgaa
     7321 attttattac cagccatttt attttccttt atgtgtctca atatgatttt taggtgttag
     7381 cgccttaatg ttatcgacat ccataacagc ccctccttga gatatttctt tttcgtggtg
     7441 taattcaatt ttaacacgtt tccctactga ttctaaaaag tccgcagtag gtgcccttcc
     7501 agctttcatc tgattaatat cagcttcact aaattgcttg ctcagtgcag aatcgtcagc
     7561 aaccgctttc cagaacgccc gacggaaaga atcaaaacta ccgaaagtct tcccgcgtaa
     7621 tttatccgca atctgggaag gaacaggagc accatccccg gtactggccc cccccatcca
     7681 gttattgcca acgttctgcc ctttgccgct cactgtaccg ggcatgttac gagggctacg
     7741 gtacatcaca tacagcggtt ttaggccgga ttccggagga aacaccagaa taatatcgtc
     7801 aaaatcgagg tcatccgcca tacgaaaagc agaaggtagt gcatctgacc acagctatgc
     7861 actccagtta ctggagatta atttcaaggc aaacccactg gatctcatct atcaccctga
     7921 ctgctggttc aatgatgaag cattgtttca tgcccggctg actaccgaag aaattggtgg
     7981 ctacttaatg aaaaaatcag gtcgatggct caacgatgca ccggatatcc aactggtgta
     8041 tgccatcccc caggatgttt atgattaacc aagtcaccat ttaccataat cccttaattg
     8101 tacgcaccgc taaaacggcc tcaggacgtt tctgacggga aaccggtaaa acaggcaaca
     8161 aaccacccga aaacccgcca gaaacgcgca ggctctgttt ttacggcatg catgagtatg
     8221 cgcgcaagtg catagcgcgg aaattcacgc cattcagcca gggtaagtcc tgtttccggc
     8281 gttcttctga tccgaaaacg gtgaacgggg acgcaattta ttgcgtggcc gtgaggcgtt
     8341 gccggcgcag ccggaaaacc ggttttcagc acgttcagga atcgggttcg tcgacggccg
     8401 gcaatcacgt tatggcaagt cgccggacga ctcaccacac gattcaccgg cgattcgaat
     8461 cgtgaattga atcaagagtc accatgaaca aaaaacgccc aaattgatga attgaatcaa
     8521 gagtcgattc atcataacaa catgataata aagcgtgatt cacatatgaa tcgtgggcaa
     8581 ttcgtaaacg attcgccata gagtcgtcca cgacttggat ctgcactttg taacacatca
     8641 ctgcacatta atttttcatt aaccaattgt tttaaataag gaatctgcaa aattttaatt
     8701 ttgcgtagtg tgtgggtatt tttagtcgtg aggagcgttc agagatctgg cgatttttgg
     8761 gaaggttcag gaactccctg ggatggccgg agaggaaaac tcaagcatgg caggatcagg
     8821 attttttcca gaatttccac cacggggaat ggggtggtga agaaggctcg cgcttgtgct
     8881 ccagaagctg catagcctga cgaagggaat caatatgctc atctcgagcc ttaacagccc
     8941 ctttaagacc atcgttttca gcttcaagta gagcgattct atgttgtaaa tcatgtaaac
     9001 caacgctgtt aatacttgta gattctgcgt cactttttac atcatatttt acagtatcga
     9061 tgacaccatc aagagaacca aacactctaa taagctctga agtatcaata actttaatag
     9121 ccttcccgtg ctttacaaca gaggtgtaac ttaacttccc actgttaatc atcttgtaaa
     9181 ttgtgggacg gctaacctta acaagccgtg ccgcttcact aatagacact ctggccatca
     9241 attaccatcc tgtaattcat ccagtctcga ggacagtgta cggaaaagat gctccgcatc
     9301 atcgtggagt ttctcacaaa ggtcggcctc aacatcgaga tcaagctcgt taagcttctc
     9361 cagcaggagc agagactgat tctggatgaa tttagccatg ttcagcgctg ttttctgttg
     9421 tttcgtcata aagggcctca gagcgtcagt gctcaatgtc agacagatct cttttccgct
     9481 tcctcgctca ctcagtcgct acgctcctgc cgttcggctg cggcgagcgg tcgcaacgcg
     9541 cacataacga catagagcag acagctcgga gagggagaag gcagcacagc ggccgttttt
     9601 ccataggctc cgcccccctg acaagcatca cagaatttga cgctcaaatc agtggtggcg
     9661 aaacccgaca ggactataaa gataccaggc gtttccccct gatggctccc tcgtgcgctc
     9721 tcctgttcct gccattcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca
     9781 ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgca
//
