LOCUS       NZ_CP104313            12047 bp    DNA     circular CON 01-OCT-2023
DEFINITION  Klebsiella pneumoniae strain KP8132 plasmid pKP81321, complete
            sequence.
ACCESSION   NZ_CP104313
VERSION     NZ_CP104313.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30711370
            Assembly: GCF_025252425.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12047)
  AUTHORS   Zhang,Q., Li,X., Zhang,Z. and Wei,J.
  TITLE     Complete Genome Sequence of Klebsiella pneumoniae strain KP8132
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12047)
  AUTHORS   Zhang,Q., Li,X., Zhang,Z. and Wei,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2022) Zhuhai Hospital, Jinan University, 79
            Kangning Road, Zhuhai, Guangdong 519000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4; FALCON v. 0.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 813.95x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/01/2023 04:52:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,843
            CDSs (total)                      :: 5,721
            Genes (coding)                    :: 5,514
            CDSs (with protein)               :: 5,514
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 89 of 207
            Pseudo Genes (incomplete)         :: 125 of 207
            Pseudo Genes (internal stop)      :: 24 of 207
            Pseudo Genes (multiple problems)  :: 31 of 207
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104313.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12047
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP8132"
                     /isolation_source="Midpiece urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP81321"
                     /geo_loc_name="China:Zhuhai"
                     /lat_lon="22.28 N 113.58 E"
                     /collection_date="2022-04"
                     /collected_by="Qinghuan Zhang"
     gene            join(11978..12047,1..1280)
                     /locus_tag="N2X67_RS26590"
                     /old_locus_tag="N2X67_26590"
     CDS             join(11978..12047,1..1280)
                     /locus_tag="N2X67_RS26590"
                     /old_locus_tag="N2X67_26590"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_260714747.1"
                     /translation="MNNVRHLHFGEPYRLDALADFSTEQARKQQAVFLGKDEQGQPVLV
                     PRDTWRKTNIQILGLPGSGKSAMATNALIRCVRDFGDAVVYFDPKGDAWAPHVFRAHCP
                     DFTLLDLRPGKPAQLNLFRDLDQYALKNLLVAGFNLSETGDVADHYRISEQKAAKLIAE
                     QFPQGANIQQVLAAAYALPEALKKDVKGLITKLENVADLSVLQTDSGIDVAGIINGGGC
                     LYVIGSMDDEAVIRVQKMLFARCAQIIIARDEFRRWPHASIMLDEIKYLLSKYVLNALG
                     TLRSRDCNLLLAHQSLGDFGQCGQDLPADFVKTTVLDNTPIRWFYRAASQESAQWAAGQ
                     TGEIRVDVERRRASREAGNVEHISGDSFIQKEARPLFDVNTLQHLPDGFAVMTGLGVAR
                     LAFSSPLRVERREISLKTFPVLAKTDPLAEYLPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            1280..2011
                     /gene="mobC"
                     /locus_tag="N2X67_RS26595"
                     /old_locus_tag="N2X67_26595"
     CDS             1280..2011
                     /gene="mobC"
                     /locus_tag="N2X67_RS26595"
                     /old_locus_tag="N2X67_26595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            2018..2548
                     /locus_tag="N2X67_RS26600"
                     /old_locus_tag="N2X67_26600"
     CDS             2018..2548
                     /locus_tag="N2X67_RS26600"
                     /old_locus_tag="N2X67_26600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(2575..2754)
                     /locus_tag="N2X67_RS26605"
                     /old_locus_tag="N2X67_26605"
     CDS             complement(2575..2754)
                     /locus_tag="N2X67_RS26605"
                     /old_locus_tag="N2X67_26605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2780..3208)
                     /locus_tag="N2X67_RS26610"
                     /old_locus_tag="N2X67_26610"
     CDS             complement(2780..3208)
                     /locus_tag="N2X67_RS26610"
                     /old_locus_tag="N2X67_26610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(3372..3686)
                     /locus_tag="N2X67_RS26615"
                     /old_locus_tag="N2X67_26615"
     CDS             complement(3372..3686)
                     /locus_tag="N2X67_RS26615"
                     /old_locus_tag="N2X67_26615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3674..3952)
                     /locus_tag="N2X67_RS26620"
                     /old_locus_tag="N2X67_26620"
     CDS             complement(3674..3952)
                     /locus_tag="N2X67_RS26620"
                     /old_locus_tag="N2X67_26620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(4127..4492)
                     /locus_tag="N2X67_RS26625"
                     /old_locus_tag="N2X67_26625"
     CDS             complement(4127..4492)
                     /locus_tag="N2X67_RS26625"
                     /old_locus_tag="N2X67_26625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(4489..4860)
                     /gene="tolA"
                     /locus_tag="N2X67_RS26630"
                     /old_locus_tag="N2X67_26630"
     CDS             complement(4489..4860)
                     /gene="tolA"
                     /locus_tag="N2X67_RS26630"
                     /old_locus_tag="N2X67_26630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(5134..5379)
                     /locus_tag="N2X67_RS26635"
                     /old_locus_tag="N2X67_26635"
     CDS             complement(5134..5379)
                     /locus_tag="N2X67_RS26635"
                     /old_locus_tag="N2X67_26635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            6024..7511
                     /gene="ltrA"
                     /locus_tag="N2X67_RS26640"
                     /old_locus_tag="N2X67_26640"
     CDS             6024..7511
                     /gene="ltrA"
                     /locus_tag="N2X67_RS26640"
                     /old_locus_tag="N2X67_26640"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            7616..>8359
                     /locus_tag="N2X67_RS26645"
                     /old_locus_tag="N2X67_26645"
                     /pseudo=""
     CDS             7616..>8359
                     /locus_tag="N2X67_RS26645"
                     /old_locus_tag="N2X67_26645"
                     /inference="COORDINATES: protein motif:HMM:NF015479.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            8455..8532
                     /locus_tag="N2X67_RS26650"
                     /old_locus_tag="N2X67_26650"
     CDS             8455..8532
                     /locus_tag="N2X67_RS26650"
                     /old_locus_tag="N2X67_26650"
                     /inference="COORDINATES: protein motif:HMM:NF015479.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_260714748.1"
                     /translation="MRFPKETGQKPVYVSVDRCFLPRPQ"
                     /name="hypothetical protein"
     gene            8946..9017
                     /locus_tag="N2X67_RS26655"
                     /old_locus_tag="N2X67_26655"
     CDS             8946..9017
                     /locus_tag="N2X67_RS26655"
                     /old_locus_tag="N2X67_26655"
                     /inference="COORDINATES: protein motif:HMM:NF023002.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_260714749.1"
                     /translation="MESRKQKEKKRRMPENKLNEEKN"
                     /name="hypothetical protein"
     gene            9736..9849
                     /locus_tag="N2X67_RS26660"
                     /old_locus_tag="N2X67_26660"
     CDS             9736..9849
                     /locus_tag="N2X67_RS26660"
                     /old_locus_tag="N2X67_26660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560790.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_114441371.1"
                     /translation="MVAGFLLAACQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     BGC             2616..12047
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(9956..10551)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.625"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1281..2008
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 agcaggcccg gaaacagcag gcggtgtttc tgggtaagga tgagcagggc cagccggtgc
       61 tggtccctcg tgatacctgg cgcaagacca atattcagat cctcggcctg cccgggagcg
      121 gcaagagcgc gatggccacc aacgcgctca ttcgctgcgt acgcgatttc ggggacgctg
      181 tggtgtactt cgaccccaaa ggggacgcgt gggcgccgca cgtcttccgg gcgcactgcc
      241 cggacttcac cctgctggat ctgcggccgg ggaaaccggc gcagctgaat ctgttccgcg
      301 atctcgacca gtacgccctg aagaatctgc tggttgccgg gtttaacctg agtgagaccg
      361 gcgacgtggc ggaccactac cgcatcagcg agcagaaagc ggcgaagctg attgcggagc
      421 agttcccgca gggggcaaac attcagcagg tgctggcggc ggcgtacgcg ttgccggagg
      481 ccctgaaaaa ggacgtgaag gggctgatca ccaagctgga gaacgtggcc gacctgagcg
      541 tcctgcagac ggacagcggt atcgatgtgg cggggataat caacggcggc ggctgcctgt
      601 acgtcatcgg ctcgatggac gatgaggcgg tgatccgggt gcagaagatg ctgtttgccc
      661 gctgcgcgca aatcatcatt gcgcgggatg agttccggag gtggccgcac gcgagcatca
      721 tgctcgatga gattaaatat ctgctgtcga agtacgtgct gaacgcgctg gggacgctgc
      781 gcagccggga ctgcaacctg ctgctcgcgc accagtcgct gggcgacttc ggtcagtgcg
      841 ggcaggatct gcccgctgat tttgtgaaaa ccacggtact ggataacacg ccgatccgct
      901 ggttctatcg cgcggccagc caggagtccg cgcagtgggc ggcgggccag accggggaga
      961 tccgggtgga cgtggagcgc cgccgggcca gccgggaggc ggggaacgtg gagcacatca
     1021 gcggcgacag cttcatccag aaggaggcgc ggccgctgtt tgacgtgaac accctgcaac
     1081 acctgcctga cgggttcgcg gtgatgaccg ggctgggcgt ggcgcggctg gcgttcagca
     1141 gcccgctgcg ggttgagcgc cgggaaattt cgctgaaaac cttcccggtg ctggcgaaaa
     1201 cggacccgct ggcggagtac ctgcctccgg ccgacggcgg cggaccaaaa gcggatgatg
     1261 attttgcggg gctttactga tgacactgga gcagtacaac gtcagccacg cgaagcggca
     1321 ggcgcggaac gcggagaaaa cacggctgac cctgcgctgg ctgcgggaag agctgtgttc
     1381 cacggcggag ctggttgcgc gacggctggg gattgcggcg gtacagccgg tttaccgctt
     1441 cctggacagt ctggtggcga aagggctgct ggtccgggcg aaatacccgg ttgacggccg
     1501 tcaggtcagc gtgtgggggc tgacgccgca cggggtggcg ttctcgttcg acgaggacga
     1561 gccgctgacg gacgttattc cgtttcagcc gtcaagggtg tcggcggcgc agctgccgca
     1621 ccggctggcc gtgcagtccc tgcgcctctc catggagggc cggggggcca ccggctggcg
     1681 gtacctgcac cgtatggccc tgaaagggat gaaagtgccg gacgcgctgg cggagctgga
     1741 cggcagaacg gtggcatttg aggtagagcg caccgtcaag agccgcaggc ggtatcagga
     1801 agtggtgtcc gggtatctgt tcaaccgccg ggcgaacggc atcgatgaaa tctggtacat
     1861 ctgccctgac cggccgacgc ttgtccgggt acagcgggcg attctggcgg tggatgaaat
     1921 cgtgaatcca cagaccggcg aggcccgcaa aacggtggag ctggaccggg agaggctgtt
     1981 cgcgtgcttt aaatttatga caacggagtg agggattatg gcgctgtaca tggtatttat
     2041 tgaggacgtg gaagacaaag acggcgagcg ctgcattatg ccaaccagcg agcatctgga
     2101 agacatggtg gtgctcgcct ggcagaccgg ggcgtatgtt ttctgttttg tcgaccgggc
     2161 ccagcatctg gcgatgatga aatccatcgg gatgtatgac gagcggctca acagcgacga
     2221 aaaagccacg tactttttca ccggtgaaaa tccgcaatgg gaacgggaca aacccacagg
     2281 aatgaagctg tgtgattacc tctatcgaaa ggtggagcgg ttcgccatcg agaacggtgt
     2341 gcaggtggtt gatgccggga agtgctcacc ggagcagatt aacgcgatga ttgaggtggt
     2401 cagcgagcgg cacggggaaa gcgcggtgaa cgagaagctg aaagcggagc tgatgcggga
     2461 atataacagc cgctatttta acgtgccggt gcctgaactg ccggaggaca aacccgcccg
     2521 ccggtggtgg ccgttcggca aaaaataacg ccagcaccgg catggccggt gctgttattc
     2581 ttccccgagc ttatcccgca tcacctgcca gagtgtttcc gcatcctcgt gaagccgttc
     2641 gcacatgtcg gccggggtat ccatgtccag ctcgttcagc ttttccagca acagcagcga
     2701 ctgatcctta atgaatttag ccatattgag ggcagttttt tcctgcttcg tcatgttctc
     2761 cattcccttc ggtaagttct cagctaaacc agccggacca gaagcgtttt ttcttctgtg
     2821 tcagttctgt tgtcaatctg tccacttcgg cctggagttt ccggcgctca tcatcgtgct
     2881 ggcggcgttc ttcttcccgg gatgttttat cttccagcat cagggtgaca gcctgccgga
     2941 gatcggccag ttccttttgc attgccagaa gctgttcctg tgggaaattt ttttgtgtat
     3001 tttcacggtg tgaaatgccg ggaatttgtt tctcagggat attcacattt ccgtatacac
     3061 ggatcagttc tgacacatcc acgacagggt tgtttttccc gtcacgtgac acggtaacct
     3121 ttccctgttt tacatgattg tatagcgttc ttctggtaat gcccgcggct tgtgctgcct
     3181 gtgaaaggtt gagcaatgtt ttcgccactg tatacccctg attttatcgg gtgtgaaagg
     3241 tgtgtaattt ctcacggtaa atgtatcgct gaattttacc cgtttttccg ttcaggatgt
     3301 ggttttctga cgcgctgcgc ttgtcaaacc ggcgacggcc acgcaatgaa ttgcgccccc
     3361 gtcatcggtt attagaggct gtctgccccc ggatacccgg attctttctg atgcctgacg
     3421 gtgacgatca caatctggtc catttcccgg tcgtggcggt agagcatcac atagccactg
     3481 tcgccaaacc cgatcaccag ttccttatat tccagcggca gaaacggcac cgggcggcca
     3541 atgtctggca atgttttcag ttgctggata gcccggacga tcacctcacc tccttttctg
     3601 gctgccagcc gatttttggt tttgagaaag tcctgaaggc gttgtaaatc gcgctgtgcc
     3661 agcgcggaaa tcgttacctg tggcatggcg gagcatcctg ctcgttctca gttccccagg
     3721 tacttatcca ggcttccgct tcttccgcag taaggtgcag acccgtttcc tgatagtgct
     3781 gccatgcggc ctgtccgtcc cgcaggtact ggctgcgctt ctcttcccgg tcgatgtatt
     3841 cggtgattgc ctccagcatt agcgcgtgcg ctgagcggtg ccggtcatcg gccagcgttt
     3901 tcagccggtc cttaagttct tcatcgagcc tgatggatgt tgctgatgac ataaaagccc
     3961 ctttgtagtc atatgtatta cacatgacta ctttaatccg tggcggcgct atttgccaga
     4021 aatacggctt ttgctgccca tttttcagct tcgcttccac taatcagggc tgctacaggc
     4081 tcactcatca tccaggacta ttgcagcctg cgggttaaag gacctgttac tggcggcaac
     4141 catcatcatt gagcttttgg gtggtgactg gtcgcggatc ctgtaagccg tcatttttca
     4201 gactgaattc aaaatttata acctgtcgca ttgccgggtg ccctgcggga tatagccagt
     4261 gcctcattgc cgtcagcgtt tcccgaaaaa aaaggtgaca gccggtaagc gacacgttgt
     4321 ccactcgtcc atcgtccaga atatcaaacg tggcggtcac ctgaccttcg atgcgttttg
     4381 ctgcggcctg taccgggtag gttggctgca gtgtggttac gggttttagt ggtatcgcat
     4441 gtaccggagt gaggaacatg gccgccagca ctgtcagagt aaaagctttc atggtctgaa
     4501 atccagaggg gcgtttttga atatttgcca ggtattttca tccggtgccg gcggaatttt
     4561 ggcgtgggta acggctgata ttgctgcttt acagagtttc ggatctcctc cttcagcaat
     4621 ggcgctgttc agagtgccat cacgttgtat ggatatccgc acggtgcaca tttttcccgc
     4681 ataggcctct ggttcaaaca tctgcatttc aacagcggtc ttgatggccg cagcgtagct
     4741 actgatttta gatgacttat ccagagtata aattttcggc ggctgggtgg tggaatgctt
     4801 acaggccgtc aggagtaagg ggctgcacaa tgccagcagg ataaagggta ttaaacgcat
     4861 cgggttgatg acctgtcagt aaaaaatgtt acatgtatat ttgtcactct ttacggccac
     4921 gttcatttac ataaacacgg cttatcgtat cgtttttaca ccaaacggcc cgccatgcag
     4981 ttgcgtgcat aggcatgcag cgggttacaa tttaccgggc gcgtttccgg cggttttccg
     5041 ggtggtttgt tgcctgtttt atcccgtagc cgccggaaac gccctcagac cgtctgagcg
     5101 gtgcgggcaa tgcgacgtta tggtaaatac cccttaccgg cggcgctgtg gctgccaggt
     5161 gaaggtggtt tccgtagcct cgtatttctc ccgacgcatt tcctgcgccc gcctggcttc
     5221 acgttcgtcc tgaatggcac gaagcaccct ttcccgcacc tgttgtttta ccgcctttgg
     5281 cagcgttttg ttccctttca ggcccatggc cagtgcctga gcgacaaagg cgtccgccag
     5341 gcgcttttct tcagcgggag tcagtttcag gtgcgtcata gtggcctccg ggcgtctcag
     5401 gtttcccgaa cagtttaacc gcaggtcctc tcctcatgcc agttagcttt cagatgtgtc
     5461 tataccatct gaaatgtaag tgtgatgaat gtcttaaata ataaaatttg ttgccatgag
     5521 aaattttcgg gaaaagacgc gatctgctta caggtggctc cgggcgcttt tttgtgcctg
     5581 aagatgagaa ctaatctcat atattgacac ctgaaaacgg gaggagtaag gtaataatta
     5641 tactgtgtat atgtacagtg tgcgccggga gaccggttag agatcaaggg gtgaaagtcc
     5701 ccgaccattg aaggaccagc aatccacaag gtccccgagt catgcgttgc ataccgcgag
     5761 gtatggggcg aagcgttgac aggggtgttg acaggccagc cattgagcca cgaaatgtat
     5821 attaaattac cgggtgccga cgttgtactg ttaacggaag gcaacatcat agggtgcgac
     5881 actgcgagtg ccacatggac ccggcggggt ctgagaccct ggcatgtcaa tacgatctct
     5941 acgcgggaac cgggagatct cccctctgac catctgccag tgtcggagat ggcccgcacc
     6001 gggaagacga ggagtcatag ccggtgatgt acggagagga gaagtcggac tcgctcatag
     6061 tagcggcgaa gcaggcgaac aacccgaaag gagcggagtc agtggagcga aggagcgggg
     6121 ccaaggggaa cgcggaacag ccacacatgc gccggacaca gagccgggaa agcatgtcac
     6181 agaggctgtc acgcgtgcgg gaagctgcga agcagcggaa gaaagaacgg tttacagcat
     6241 tgttccacct gctgacagtc gaagcactgg aagccgcatt cctctccctg agcaggaaag
     6301 cggccgccgg agtggatggc atcaggtgga tggactacgc cggaaacatg aagaacaaca
     6361 taacagatct gcaccggagg ctacatcagg gcagctacag ggcgcagccc ggcaggcgtc
     6421 actacatccc aaaagcggat ggaaaacaac gcccgctcgg catcgcctcg ctggaggaca
     6481 agatcgtcca gtatgcgctg gtgaaaatcc tgaacgcagt ctatgaaaac gactttatgg
     6541 ggttctcata cgggttcaga cccgggcgaa gccagcacga tgcactggac gcactggcca
     6601 cagggctggt acgcactaac gtaaactggg tactggatgc cgacatcagt cagttcttcg
     6661 acagggtgag ccacgaatgg ctgatcaggt tcacagagca tcggatcggc gaccggaggg
     6721 taatcaggct catacgtaag tggctcacag ccgggacgtc ggaggagggt caatggcgag
     6781 caacggagga aggcacccca cagggtgcgg tcatctcacc gctgctggca aacatatacc
     6841 tccactacgt cttcgatctg tgggcgcatc agtggcgacg tcgctatgcc acaggcaatg
     6901 tggtaatggt cagatacgcc gatgacatcg tcatcgggtt cgacaaacga tacgatgccc
     6961 ggcgcttccg tatagccatg cagcgcagac tgagggagtt cggactcacg gttcacccgg
     7021 agaaaacccg tctgatggag ttcggccgct tcgctgccga aaaccgtgcc atcaggggaa
     7081 aaggcaaacc agaaacgttc aacttcctcg ggttcacgca catcagcggg aaagatcgca
     7141 acggcaggtt catgctgata cgaaagaccc gccgggatcg gatgacggca actctgaaag
     7201 ccatcaaaga cggtctgcga aggcgctggc attactcaat ccccgaacag ggaaaatggc
     7261 tcaggagagt ggttcaggga tacctgaact atcactcggt accgggcaac ttccccacca
     7321 tgcagaagtt caggacacac gtaacaaacc tctggcgccg ggcgctcagg cgcaggagcc
     7381 agaaggatga tacgacctgg acgaaagcaa acaaactggc agccgcatgg ctaccaaggg
     7441 ttcgggttct tcatccatgg cctgtggagc ggttcaccgc cagacacccg aggcaggagc
     7501 ccggtgcgta aatcgcgcac gccgggatct gtgcgggggg tatccggtaa cgggtatccc
     7561 taccgcgaca tgttggcttt aatttattgt tttaaatgtc aaagaggaaa acggtatgag
     7621 tggtggagac ggtcgaggtc cgggtaattc aggtctggga cataatggtg gtcaggccag
     7681 tgggaatgtg aacggtacgt ctggtaaagg tggcccttca tcaggtggtg gtacggatcc
     7741 aaacagcggg ccgggctggg gtacgacgca tacgccgaat ggagatattc ataactataa
     7801 tccgggggag tttggtaacg gcgggagtaa acccggtggt aatggtggta acagcggcaa
     7861 tcatagcggt agctctggtg gcggacagtc ttcggccact gcgatggcct tcggtctgcc
     7921 tgccctggct actccggggg ctgaaggact ggctttatca gtttccggcg atgcgttgtc
     7981 ggccgccgtt gctgatgtgc tggctgccct gaaggggccg tttaagtttg gtctgtgggg
     8041 gattgcgatc tacggtgtgc tgccttctga gattgcaaaa gatgatccga aaatgatgtc
     8101 aaaaattatg acgtcattac cggccgatgc ggtgacggag actccggcaa gtactttacc
     8161 actggaccag gcgacggttc gtgtcagaca acgggttgtg gatgtggtga aggatgagcg
     8221 gcagcatatt gcggttgtcg caggtcggcc aatgagtgtc cctgtggtgg atgcgaaacc
     8281 gacaaaacgt ccgggggtat tcagtgtgtc gattccgggt ctcccgtctc tgcaggtgag
     8341 cgtaccgaaa ggtgttccga cagcgaaagc cccgccaaaa ggcattgttg ctgaaaaagg
     8401 tgattcacgt ccggctggtt ttacagccgg ggtggtaact cccgtgaggc cgttattcgt
     8461 ttcccgaaag agaccggaca gaagccggtt tatgtgtcgg tggacagatg tttcttaccc
     8521 cggccacagt aaaacagcgt caggaggaag aaaagcgtcc gccagcaggc aagggacgcc
     8581 gctcatccgg aagaagggct aacagaaaca atgataacgc gaaagctgag ctggatgccg
     8641 aagataaaaa tattttgcga ccttaaacag ccgcattgca tcgacagaga aggcgcttcc
     8701 cgtgcaaggg ccgtgctgtc aggaagccga taaaaagtaa aagaggcaga ggcaaataaa
     8761 gatgattttg tgacttataa ccccgccaca tgaataatgg tccgggtggc aggatcacag
     8821 gttcgcttat ctttgataag gatattcaga atcagaatga gaaattaaac aaaagcggct
     8881 caaggcatct taaaacgcaa tgaatgaatt ccttatccag agataagggc tgcattacac
     8941 gggcgatgga gagcaggaaa caaaaggaaa aaaagcgaag gatgcccgaa aataagttaa
     9001 atgaggaaaa gaactaagcc tcgcagggga ctaagactac ggccatgatt atcataccag
     9061 tacctaaaac agaagacata aaggacatgg agagttatag cgaggtgatc ctaacacacc
     9121 taaacaaggt ggtggtgtag cgggcccgat ggtatggcga taaaaaacgt aaaatttatg
     9181 aatgggattc ccagcagcgg tgagcttgaa gggtatcgtg ccagtgattg gcgaacacta
     9241 ggcgcattcg atcccaaaac gtaagcaggt taagggccag atccaaacgg aaacattaaa
     9301 aaaatctttg agaggtaaat attgggctta aattaagttt gacatgtttt tgataaaaaa
     9361 ctgaagagtt tttaaaggga agattattct aaagacttgt gacgatggtg ttttattgta
     9421 ttttattttt ctggggtttg gttcaccccc cacatttgaa agcttggaat gcctttaaag
     9481 gataaattta ataatgggtg aagtttgatt gaagacagaa tgggttccat ttactacagc
     9541 cattttttaa aaatctaata aaacctgagg tggagaattg ttatataagc ttttgcatat
     9601 cggacggtac tgggtaacca aaaaatatca aggatgattt gaaatactga cttccggctg
     9661 ttcagatgaa tgccggagtc tttaaaatac aggaaagtat atgaaaaagg tacagactat
     9721 tttcgtttaa tatagattgt tgctggcttt cttctggccg catgtcaggc aaattatatc
     9781 cgggatgttc agggcggaac ggtggcacca tcgtcctcct ctgaactgac ggggatcgcg
     9841 gttcagtaga aaagatcaaa aggatcttct tgagatcctt tttttctgcg cgtaatcttt
     9901 gccctgtaaa cgaaaaaacc acctggggag gtggttttga atcgaagttt aaagtcagtt
     9961 ggggaactgc ttaaccgttg gtaactggct ttcagcagag cagcagcaac caatctgtcc
    10021 gtccattgta gccggacttt tggcgcacac gattcaagag caacgcgcgg ggttttagct
    10081 aaacaaatcc actgcgaact cccagttccc aatggcctgc tgcatggcgg ttttgcgtgg
    10141 ctttttcccg ggttggactc aagtgaaaca gtgtaccgga taagcgcagc agtccgggtg
    10201 aacggggagt tcttgcttac gcccagcttg ggagcgaacg acctaccacc gacctaaacg
    10261 agataccagt gtgtgagcta tgagaaagcg ccacacttcc ccgcaaggga gaacggcgga
    10321 caggtatccg gtaaacggca gggtcgaaca ggagagcgca aaggagcgac ctaccgtaaa
    10381 cggtggggga tctttaagtc ctgtcgggtt tcgccgtact gtgcagattc atggttgagc
    10441 ctcacggctc ccacagatgc cccgaaaaag cgtcctgttt atgtgaaact ctggcaggag
    10501 ggcggagcct atggaaaaac gccaccggcg cggccctgcc gtttgcccca cagttagtcc
    10561 cctgctttat ccacggaatc tgtgggtatc ttgttatgtg tcgcagcgcc cgccgcagtc
    10621 tcaacgcccg gagcgtagcg accgagtgag ccgaggaggc gcaaattccc cgcaacctgc
    10681 agacggtcac cgaccgcgct tgatgtggga tattgtcccc tattaagtct tctattcccc
    10741 tgacgatcaa ttatcagata ggtgaaccat gttccggttt ttcgctgaac atttttacgt
    10801 taaacctatc tctgttgctt tttctccttg cggagaaatg caagtctccc ctgctggggc
    10861 gtggctgact tcaaggggaa aagacctgct tacgctggct ggacgccacc gacgcacgga
    10921 cttttccctc tttctggctc tgtccggctg gacgccggcc acgccagaaa ttcacgtccg
    10981 gccctccgtc agctcacgcc taccacccat caaaaatcca ggtgctggac gcaacctgac
    11041 aagcagcaac gctgctctga attttgcggg tggtcgccgc tcgctatttg cgaacctctg
    11101 acggggccgg aacaaccgga aaagaaccgc actgtggttg ccgccttttc ctcgcaccac
    11161 gcctggggcg tgcctcgttg tcctccgggg ctgcgccttt tccgcatggg gccggaaaga
    11221 ggctggcctc cgcgttgaaa gctgacgcaa tcaccacgga cgtgaccgcc gccgaccatg
    11281 ccttggggca tgtcgcggct ttggcagcgg gggctgcgca acacgccggt aaacccgtct
    11341 ctgaaggaga gagattgcat gtttaatacg gattcgcttg tcctggcagt ggccatatcc
    11401 cgggaggact ggcccggcga tttacccgtg tgtcagctgc tgtaccggag tatacagtct
    11461 ggccgttttt ttctggttat cggcctgttt tttcggtcta ctggctgaac tctgtgccgc
    11521 gcgaatgctt ttttggtggg gctgagccat aaggcgcggc tggagtatgc cgacgctata
    11581 agcgcgataa taaccgactg tgccattttc ggtgctggcc tcgctggatt cacccggcgg
    11641 atggtcaggg cacggcgaca tgtctggaac tacgccgact ggctgaccgg cgtgccggtc
    11701 tggcccttct ggggggctgg ttgtcgccgt gttttacgag agctggggag aaaccgtcct
    11761 gcgccgcccg gacctctatg cgaacgcaga tttattacct gctgcatagc ggctaacctg
    11821 ttcggaagcc tggtgtgcgg aggcttgtcg ccggcgtccc ttcggcgcgg ctgctggcga
    11881 tttcctgctg cttgatggtc attttgcggc cgctgacgag gcggtgaacg cgaaactgaa
    11941 tccaggagag ccggcgcagc agccagcgga acgagaaatg aacaacgtcc gccacctgca
    12001 tttcggggag ccgtaccggt tagacgcgct ggccgatttt tccacgg
//
