LOCUS       NZ_CP104201             3991 bp    DNA     circular CON 30-SEP-2023
DEFINITION  Klebsiella pneumoniae strain CHN23026 plasmid pCHN23026_5, complete
            sequence.
ACCESSION   NZ_CP104201
VERSION     NZ_CP104201.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30636576
            Assembly: GCF_025244645.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Hu,H., Yu,Y. and Jiang,Y.
  TITLE     Prevalence and Colistin resistance characteristics of
            Carbapenem-resistant Klebsiella pneumoniae isolates from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Hu,H., Yu,Y. and Jiang,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2022) Infectious Disease, Sir Run Run Shaw
            hospital, East Qingchun Road, hangzhou 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 110.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/30/2023 03:52:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,306
            CDSs (with protein)               :: 5,306
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 40 of 107
            Pseudo Genes (incomplete)         :: 59 of 107
            Pseudo Genes (internal stop)      :: 17 of 107
            Pseudo Genes (multiple problems)  :: 9 of 107
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104201.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CHN23026"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCHN23026_5"
                     /geo_loc_name="China: Taiyuan"
                     /lat_lon="37.87 N 112.55 E"
                     /collection_date="2018-10-01"
                     /collected_by="Huhuangdu"
     gene            59..171
                     /locus_tag="N3931_RS27625"
                     /old_locus_tag="N3931_27625"
                     /pseudo=""
     CDS             59..171
                     /locus_tag="N3931_RS27625"
                     /old_locus_tag="N3931_27625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(398..1372)
                     /locus_tag="N3931_RS27630"
                     /old_locus_tag="N3931_27630"
     CDS             complement(398..1372)
                     /locus_tag="N3931_RS27630"
                     /old_locus_tag="N3931_27630"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_114269875.1"
                     /translation="MAFFNLLFVASLICFGGVMGSYSRGCRDSQNKFSKDKDENNKRAS
                     VYRFGIASAFICVPFISSFLHVDYSSIIFPVADGGGTKFIEQILLLISVSGISAYLGYA
                     LLDGLANKVLKEQVDGIDKKQQDLEAEQDEFKDELDRSKELIEQLEMDKKTTKFELGYF
                     KAISAVDKAESMMSIPDEALSVKKKLTEALDAVTESLSLVKREDVAKDDYDKLLVLKAY
                     ILKRLDRIDDALSITDELLMSNEDNPILIYNKACYQYILRRCQADNSDIKDMIRRALTI
                     KVTDPEFIRRQEKIRTKVIGNKDNDLEGLFTDAELEELKVAIK"
                     /name="hypothetical protein"
     gene            complement(1953..2225)
                     /locus_tag="N3931_RS27635"
                     /old_locus_tag="N3931_27635"
     CDS             complement(1953..2225)
                     /locus_tag="N3931_RS27635"
                     /old_locus_tag="N3931_27635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004897311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_019405140.1"
                     /translation="MATDAQVKEINALIKKYPDSCSICHEVYDEDDVTYTVFGYDRKGK
                     IQVTTGCCAGMLTEPVLLGVCGCFDPEERDEIMQNHPMAKQFFTE"
                     /name="hypothetical protein"
     gene            complement(3029..3451)
                     /locus_tag="N3931_RS27640"
                     /old_locus_tag="N3931_27640"
     CDS             complement(3029..3451)
                     /locus_tag="N3931_RS27640"
                     /old_locus_tag="N3931_27640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_040214596.1"
                     /translation="MAIVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3451..3642)
                     /locus_tag="N3931_RS27645"
                     /old_locus_tag="N3931_27645"
     CDS             complement(3451..3642)
                     /locus_tag="N3931_RS27645"
                     /old_locus_tag="N3931_27645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_040198051.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLDKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          2..326
                     /name="rep_cluster_2374"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.395"
                     /coverage="94.0"
                     /evalue="5.5199999999999996e-67"
ORIGIN
        1 tctcattcca tgcctgatac tcggctccgg gaaggcagtt cgctccaagc tggactgtat
       61 gcacgaaccc cccgttcagt ccgactgctg cgcccgttcc agtaactatc tcttgagtcc
      121 aacccggaaa gacacgactt atcgccattg gcagcagcca ctggtaattg atttagagga
      181 gttagtcttg aagttatgcg ccagttaagg ctaaactaaa cgaactgttt tggtgactgc
      241 gctcctctaa gccagttacc tcggttcaga aacttgccgg ttactgaacc ttcgaaaaac
      301 caccttgccg ggtggttttt tcgtttgagg ggtagagtac gctgcacctc aaaggatcgt
      361 caaggatctt tttggaattc atagagctag ccactgccta tttgatagcc acctttaatt
      421 cctccagctc agcgtcagta aacaaccctt caagatcatt atctttattt cctatgacct
      481 tggttcttat tttttcttgc ctgcgaatga attcaggatc tgtaacttta atagtcagag
      541 ccctgcgaat catatcctta atatcggagt tatcagcttg gcaacgacgc aaaatatatt
      601 gatagcaagc cttattgtaa ataagtatag ggttatcctc attactcata agtaactcat
      661 ccgtaattga cagagcatca tcaatacgat caagtctttt caaaatataa gctttaagca
      721 ctaagagctt gtcgtagtca tccttagcta catcctctct tttaacaaga gacaatgatt
      781 ccgttacagc atctaatgcc tcagttagtt ttttcttaac agaaagagcc tcatccggaa
      841 tggacatcat tgattctgct ttatccacag ctgaaatggc tttaaagtat cctagctcga
      901 attttgttgt tttcttatcc atttcaagct gttctatcag ctccttgctc ctatcaagct
      961 catctttaaa ctcatcttgt tcagcctcca aatcttgttg ctttttatct ataccgtcta
     1021 cttgctcttt taatactttg ttggcaagac catccagtaa tgcgtaccca agatacgcag
     1081 agatccccga aacagaaata agaagcaata tttgttctat gaacttagtt ccaccaccat
     1141 cagccacagg aaatattata cttgagtaat caacatgaag aaatgatgat atgaaaggaa
     1201 cacaaataaa tgcggatgct ataccaaatc tataaacact agctctttta ttattctcat
     1261 ccttatcttt tgaaaattta ttttgggagt ctcggcaacc tctggaatag ctccccataa
     1321 caccaccaaa acaaattaga gatgcaacaa acaataggtt aaaaaaagcc atgagagaat
     1381 ccttgcgcaa agcaaacaat aaaactttgt tcaggtaagt taagataagc tcaatcatat
     1441 actaaccaat aacaaaaaag gcagccttgg ctgccttttt ctgctgactt gacacttaca
     1501 agtctggacg ctcatcgtga agtttgattt ttttcataga gcacctctca ttaattgttc
     1561 ctttgcaagc ccatgcttgc ttagcgctga atatagggca taaacccagc actcacaaca
     1621 acaaaaatgc ttaaaatgat aaaatcaacc catgtattga cattgggtga aaaaaaaagt
     1681 ataatgcact ggtagccaaa ggggcggggc ttcgcttatt cctaaggaat aagcgaagcc
     1741 ccgccccttt ggctaccata attaaatgat tgaatttaaa ggattttttt acaataacat
     1801 ccttcctctc aatagtcata taaacatgcc ccatgtattc gtcattctgg atttttcaaa
     1861 agaatgaatg cgtgcattta ccataacgcc tcatttcacg cagatatgaa aaagcccgca
     1921 caaggcgggc ttcagactca acagatacgg aatcactctg tgaaaaactg ctttgccatg
     1981 gggtgattct gcatgatctc gtcacgttct tccgggtcaa agcacccaca gacacccagc
     2041 agaaccggct ctgtcagcat tccggcacag catcccgtgg tgacctgtat tttacctttc
     2101 cggtcatacc caaaaacggt gtaggtcaca tcatcctcat catagacttc atgacagatg
     2161 gagcatgagt ccgggtattt tttgatgagt gcattaattt cttttacctg agcgtcagta
     2221 gccaaaacat ctccctgagt cactgattat cacaatccgc tgactctaac acagactcag
     2281 gcatccgccg ttgatttacc ataacgtccc attacacgca ccgctaaaac ggcctcagaa
     2341 cgattctggg gcgtaaccca ttaaatccta cgtcttaccc cctgaaaacg cctcaaaatc
     2401 gctccagggc gttttacggg catgaatcgt tatgcacttg actgactgtc tggggatttc
     2461 agcctgaatt caggccaggc aactcaaaac ccgcaccaaa ccagactgaa aacggtgaac
     2521 gggtgcgcaa tttattgcgt ggccgtgaag cgttgccggc gcagccggaa aaccggtttt
     2581 cagcacgttc aggaatcggg tttgtcgaga ccagcaatca cattatggca agtcgccgga
     2641 cgactcacca cacgattcac cggcgattcg actcgtgact tgactctaga gtcgccatgg
     2701 tcaaaaagcc accaacccgg caagtcgact ctagagtcaa gtcatcataa cgtactgatt
     2761 atatggcgtg attcacatat gaatcgtggg caagtcatcg gcgattcgcc atagagtcgt
     2821 ccacgactcg gattcacctt acaaaaaata aaattgcgta atttaaaata gataactgat
     2881 tgaaaataaa ggtctatctg caaaatttta attttgcgta gtgtgtgggt atttttagtg
     2941 gtgaggagcg ttcagggatc tgacgatttt tgggaaggtt caggaactcc ccggaatggc
     3001 cggagagcaa aatggaagca tggcgggatc aggatttttt ccagaatttc caccacgggg
     3061 aatggggtgg tgaagaaggt tcgtgcttgt gctccagaag ctgcatagcc tgacgaaggg
     3121 aatcaatatg ctcatctcga gccttaacag cccctttaag accatcgttt tcagcttcaa
     3181 gtaaagcgat tctatgttgt aaatcatgta aaccaacgct gttaatacct gtagattctg
     3241 cgtcactttt tacatcatat tttacagtat cgattacacc atcaagagaa ccaaacactc
     3301 taattagctc tgaagtatca ataactttaa tagctttccc gtgctttacc acagaggtgt
     3361 aacttaactt cccactgtta atcatcttgt aaattgtggg gcgactaacc ttaacaagcc
     3421 gtgccgcttc actaatagac actatggcca tcagttacca tcctgtaatt catccagtct
     3481 cgaggacagt gtacggaaaa gatgctccgc atcatcatgg agtttctcac aaaggtcggc
     3541 ctcgacatcg agatcaagct cgttaagctt atccagcagg agcagagact gattctggat
     3601 gaatttagcc atgttcagcg ctgttttctg ttgttttgtc ataagaagcc tcagagcgtc
     3661 agtgctcaat gtcaaactag tctcttttcc gcttcctcgc tcactcagtc gctacgctcc
     3721 agccgttcgg ctgcggcgag cggcaggagc gtgtacataa aaacgtaggc aaccaagata
     3781 tgaggcagaa aagcaaaggc cgccgaacgg ctgtttttcc ataggctccg cccccctgac
     3841 aagcatcgcg gaaacggacg ctcaaatcag tggtggcgaa acccgacagg acttaaagat
     3901 accaggcgtt tccccctggt ggctccctcg tgcgctctcc tgttcctgcc tttcggttta
     3961 ccggtgtcat tccgctgtta tggccgcgtt t
//
