LOCUS       NZ_CP104200             4823 bp    DNA     circular CON 30-SEP-2023
DEFINITION  Klebsiella pneumoniae strain CHN23026 plasmid pCHN23026_4, complete
            sequence.
ACCESSION   NZ_CP104200
VERSION     NZ_CP104200.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30636576
            Assembly: GCF_025244645.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Hu,H., Yu,Y. and Jiang,Y.
  TITLE     Prevalence and Colistin resistance characteristics of
            Carbapenem-resistant Klebsiella pneumoniae isolates from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Hu,H., Yu,Y. and Jiang,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2022) Infectious Disease, Sir Run Run Shaw
            hospital, East Qingchun Road, hangzhou 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 110.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/30/2023 03:52:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,538
            CDSs (total)                      :: 5,413
            Genes (coding)                    :: 5,306
            CDSs (with protein)               :: 5,306
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 40 of 107
            Pseudo Genes (incomplete)         :: 59 of 107
            Pseudo Genes (internal stop)      :: 17 of 107
            Pseudo Genes (multiple problems)  :: 9 of 107
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104200.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CHN23026"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCHN23026_4"
                     /geo_loc_name="China: Taiyuan"
                     /lat_lon="37.87 N 112.55 E"
                     /collection_date="2018-10-01"
                     /collected_by="Huhuangdu"
     gene            60..173
                     /locus_tag="N3931_RS27705"
     CDS             60..173
                     /locus_tag="N3931_RS27705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            782..1075
                     /locus_tag="N3931_RS27590"
                     /old_locus_tag="N3931_27590"
     CDS             782..1075
                     /locus_tag="N3931_RS27590"
                     /old_locus_tag="N3931_27590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012535809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048290307.1"
                     /translation="MTIVISKEIEEKLTTKHKVTKKEVHESLANLNGNLLIDNREQHKT
                     NPPTNWFIAETNQRRELKVCFMIIDGDIHIKTAFEPNEIERKIYATHGKKTT"
                     /name="hypothetical protein"
     gene            1272..1640
                     /locus_tag="N3931_RS27595"
                     /old_locus_tag="N3931_27595"
     CDS             1272..1640
                     /locus_tag="N3931_RS27595"
                     /old_locus_tag="N3931_27595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048290306.1"
                     /translation="MKTTSEFARDAQKWDDRELGASENHVEVASMADMSAFNDALSLQA
                     ISIRLQKNLVSDLKSIAESYGIGYQPMVRDLLQRFVIAERKQALRRELDKLNEREEIHQ
                     QEDTVPVDEFIESIRKQA"
                     /name="hypothetical protein"
     gene            1696..1824
                     /locus_tag="N3931_RS27600"
                     /old_locus_tag="N3931_27600"
     CDS             1696..1824
                     /locus_tag="N3931_RS27600"
                     /old_locus_tag="N3931_27600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012587681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072201952.1"
                     /translation="MSLKPGPKRIADSTGQPDRRQRDNKKTPGNTDKLKPSKSSSK"
                     /name="hypothetical protein"
     gene            1894..2157
                     /locus_tag="N3931_RS27605"
                     /old_locus_tag="N3931_27605"
     CDS             1894..2157
                     /locus_tag="N3931_RS27605"
                     /old_locus_tag="N3931_27605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="WP_072072086.1"
                     /translation="MAAHTSETVKFLQSVSSDQTWWKSRYNPGDSVPYSGIYRCTVCGK
                     EITSNGSDPFPPQNHHQHSQQQAIKWQLIVRTDTKGDRFGIK"
                     /name="protein L"
     gene            complement(2889..3152)
                     /locus_tag="N3931_RS27610"
                     /old_locus_tag="N3931_27610"
     CDS             complement(2889..3152)
                     /locus_tag="N3931_RS27610"
                     /old_locus_tag="N3931_27610"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048290305.1"
                     /translation="MVNLNDICRSKKAAWIGGFFIFLARQNGIFDTNSGWLLLRVQFLS
                     RTVLRAVLLYLQLLRYSMGMYEADRGFQTFFITPFFYQQTWI"
                     /name="hypothetical protein"
     gene            complement(3880..4281)
                     /locus_tag="N3931_RS27615"
                     /old_locus_tag="N3931_27615"
     CDS             complement(3880..4281)
                     /locus_tag="N3931_RS27615"
                     /old_locus_tag="N3931_27615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_064175096.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKTGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPTPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4281..4472)
                     /locus_tag="N3931_RS27620"
                     /old_locus_tag="N3931_27620"
     CDS             complement(4281..4472)
                     /locus_tag="N3931_RS27620"
                     /old_locus_tag="N3931_27620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          2..173
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.422"
                     /coverage="92.0"
                     /evalue="5.16e-83"
     Typing          4643..4823
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.628"
                     /coverage="87.0"
                     /evalue="1.88e-76"
ORIGIN
        1 atctcattcc acgcctgaca ctcagttccg ggaaggcagt tcgctccaag ctggactgta
       61 tgcacgaacc ccccgttcag tccgactgct gcgccttatc cggtaactat cgtcttgagt
      121 ccaacccgga aagacatgca aaagcgccac tggcagcagc cactggtaac tgatttagag
      181 gagatagtct tgaacttatg cgccggttaa ggctaaacta aacgaacagt tttggtgact
      241 gtgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa
      301 aaaccgcctt gcagggcggt tttttcgttt tcacagcagt gagattacga cgactaacac
      361 atgatctcaa gaagatcctc agcctaagat tgtgcctttt tctttttgct atctgcagca
      421 cctttaacac gacaaactct acaccacgtc tgaggagcta cgttgccgcg tagatttcta
      481 aaaccaaata gagcgtcaac ctcttgatag gtcaaagcta cgactgggtt caatcgataa
      541 tcatagtcag aacaatgaca tgaatgaggc aatgctattg tattgctcat aacttcttct
      601 atatctatct ttcataacca atatcaacgg accaaaggct gcgaagaagc aatcgaaaca
      661 gcctatgtat atacacatag aaaatgaaaa caccttaaca atcaagccat ggattgacaa
      721 atgtaaattt tgtatataca atgaattcaa tttcacaaca aaggccgagg catcatgaaa
      781 tatgacaatt gtaatcagta aagaaattga agaaaaacta accacaaaac ataaagtaac
      841 taaaaaagaa gtacatgaaa gccttgcaaa ccttaacggt aacctgttga tagataacag
      901 agaacaacac aaaaccaatc ctccaaccaa ctggtttatc gctgaaacta accagcgtag
      961 agagttgaaa gtgtgtttca tgattatcga tggcgacatt catatcaaaa cagccttcga
     1021 accgaacgaa atagaacgta aaatctatgc tacgcatgga aaaaagacga cataacagcg
     1081 ctaaataagt aattgttttg caaggcctta ccatgtaaga ccttgcaaaa atcttacatc
     1141 agcgaaagaa ctaaaatgaa aatgaagctg aacctgattt ctcaacatag aagttccttt
     1201 cggaactaga taaagcataa atattgcagt taatcctgat ctgcaggaaa cagcacctag
     1261 aggtctatac aatgaaaact accagtgaat tcgcacgtga tgcacaaaaa tgggatgatc
     1321 gagagctcgg cgcctctgag aatcacgtag aagtagcatc gatggctgac atgtcagctt
     1381 tcaacgatgc actatcactg caagccatct caataagatt acagaaaaat cttgtaagcg
     1441 atttaaaatc gatagctgaa agctatggta taggttatca accaatggtt agagaccttc
     1501 tgcaacgatt tgttattgca gaaagaaaac aagcattacg tagagagttg gacaagctca
     1561 atgaacgaga agaaatacat cagcaggagg acacagttcc agttgatgaa ttcattgaaa
     1621 gcataagaaa acaagcatga aagagcttac catctagata gaaaaaattt catcgcgttt
     1681 gaaaggagga ttatcatgag tcttaaacct ggtccaaaaa gaatcgcaga ttcaacaggg
     1741 caacctgata gacgccaacg tgacaataaa aaaacaccgg gaaatactga taaattaaaa
     1801 cccagtaaat cctcaagtaa atgaatcagt taaaagccgc cttttaggcg gcttttcatt
     1861 gtcagtccta taaattcaga aaaggaaagc taaatggctg cacataccag cgaaacagtg
     1921 aagtttttac aaagcgtcag tagcgatcaa acatggtgga agtcacgata taatccagga
     1981 gacagcgttc cctattctgg aatttatcga tgtacagttt gcggaaaaga gatcaccagt
     2041 aacggcagcg acccgttccc accacaaaac caccatcagc acagtcagca acaagcaata
     2101 aaatggcaat tgattgtcag aacggacact aaaggtgacc gtttcggaat caaatgattg
     2161 agagccctcc ggggcttttt tttgcacctg aagctcgaag atatttacca taacatccca
     2221 gccccgcact tcttgtatgc taacaatcgt tacgctgcat gagcgcacga acgagatgca
     2281 tgagtctttc tcgccctcca aaaaagttaa acaaaaaaaa acaaatcaga ttaagtcatt
     2341 gtatttaatg cattaattaa aatttacagc ctttagataa tagaaaaata aattaaaaat
     2401 caatcactta aaaaaataca caatttactt tacttgttta gtttgtcagt tattataaat
     2461 gaatattagt tactgatatt aatggtggtt tttcatggcg gttgttgtta tctatcgggc
     2521 taaatgcatg gcttgtcggc atgaatggac tagtaggagt gggtttggaa caccgaacta
     2581 ctgccctaaa tgtcgttcaa aattaattag ttgcagtgca attaagtagg tcaaaatgac
     2641 atcaaaacac aaagggttat gtcctttttg taaagctgaa gttatacctc gtgtaattca
     2701 agaaaaccat gtacgacgag ataagtgcga atgccctgag tgcaaagaag caatttacat
     2761 atgtcgcgct ccggggtgtg agaattacgc taagggtggt cgtttgtatg acgatgagtt
     2821 gtgtccgtca tgtacatcag gtattggcgg cggagtcggc acattaggca tgatcgcctt
     2881 gggtgcagtc atatccacgt ttgttggtaa aaaaatggag tgatgaagaa tgtctggaaa
     2941 cctcggtctg cctcatacat gcccatgctg taacgtaaca actgcaaata cagcaggaca
     3001 gctctcagca ctgttcggct taagaactgt accctcagga gcaaccaacc agagttggtg
     3061 tcgaaaatgc cgttctgccg cgccaaaaaa atgaaaaagc cgcctatcca ggcggctttt
     3121 ttgcttctgc agatatcatt tagatttacc ataacgtctt cttgtacgca ccgcaaaaac
     3181 ggcctcaagg cgtttctgac gggaaaccgg taaaacaggc aacaaaccac ccgaaaaccc
     3241 gccagaaacg cgcaggctct gtttttacgg catgcatgag tatgcacgca agtgcatagc
     3301 gcgaaaattc acgccattca gccagggtaa gtcctgtttc cggcgttctt cctatccgaa
     3361 aacggtgaac ggggacgcaa tttattgcgt ggccgtgaag cgttgccggc gcagccggaa
     3421 aaccggtttt cagcacgttc aggaatcggg ttcgtcgacg gccggcaatc acgttatggc
     3481 aagtcgccgg acgactcacc acacgattca ccggcgattc gaatcgtgaa ttgaatcaag
     3541 agtcaccatg aacaaaaaaa acccagagtg atgaattgaa tcaagagtcg attcatcata
     3601 acaatatgat aataaagcgt gattcacata tgaatcgtgg gcaattcgtc agcgattcgc
     3661 catagagttg tccacgactt ggatctgcac tttgtaacac atcaccgcac atttattttt
     3721 cattaaccca ttgttgtaaa taaggaatct gcaaaatttt aattttgcgt ggggtgtggg
     3781 tatttttagt ggtgaggagc gttcagagat ctgacgattt ttgggaaggt tcaggaactc
     3841 cccgggatgg ccggagagga aaactgaagc atgacggggt caggattttt tccagaattt
     3901 ccaccatggc gtcggggatt cagttaattt tgcctggtga ttttcaagaa ggagcatggc
     3961 ctgtttcagg ctgtcaatgt gcgaatcctt ggacaaaagt aaggtgttca ggtgttcaat
     4021 ctcctgtttc agatgacgaa tcacctgttc actctggtca gcagaggctg tatcacgctg
     4081 ttcagagacc tgtccagaat ggtgttcacc cttcggttgc tcaaagggac caaagacacg
     4141 taaaagctct gaagtatcta ccatttttgc attgcgctca ccactacagg tggacaggtc
     4201 acctgtttta attaacctgt gcagtgttgt cctgctctta cccgtcagtc gggcagcctc
     4261 actaatagac actctggcca tcagttacca tcctgtaatg catccagtct cgaggacagt
     4321 gtacggaaaa gatgctccgc atcatcgtgg agtttctcac aaaggtcggc ctcgacatcg
     4381 agatcaagct cgttaagctt ctccagcagg agcagagact gattctggat gaatttagcc
     4441 atgttcaggg cagttttctg ttgtttcgtc ataaggggcc tcagggcgtc agtgctcagt
     4501 gtagggatgt tctcttttcc gcttcctcgc tcactccgtc gctacgctcc tgccgttcgg
     4561 ctgcggcgag cggacgggac acacagaaga gtcatcgcga taaccaggaa gaaaagagcc
     4621 tgtggaaaag gccacggcgt tgccgttttt ccataggctc cgcccccctg acaagcatca
     4681 cagaaacgga cgctcaaatc agcggtggcg aaacccgaca ggacttaaag ataccaggcg
     4741 tttccccctg gtggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc
     4801 attccgctgt tatggccgcg ttt
//
