LOCUS       NZ_CP104034             5596 bp    DNA     circular CON 25-SEP-2023
DEFINITION  Klebsiella pneumoniae strain 150040X0B1 plasmid p2_150040X0B1,
            complete sequence.
ACCESSION   NZ_CP104034
VERSION     NZ_CP104034.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30672525
            Assembly: GCF_025152765.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Yin,X., Liu,L., Feng,Y. and Zong,Z.
  TITLE     The complete genome of non-phage-resistant Klebsiella pneumoniae
            strain 150040X0B1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Yin,X., Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2022) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Trycycler v. 0.5.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/25/2023 03:15:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,823
            CDSs (total)                      :: 5,701
            Genes (coding)                    :: 5,512
            CDSs (with protein)               :: 5,512
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 189
            CDSs (without protein)            :: 189
            Pseudo Genes (ambiguous residues) :: 0 of 189
            Pseudo Genes (frameshifted)       :: 65 of 189
            Pseudo Genes (incomplete)         :: 135 of 189
            Pseudo Genes (internal stop)      :: 20 of 189
            Pseudo Genes (multiple problems)  :: 28 of 189
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104034.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="150040X0B1"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2_150040X0B1"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2020-06-12"
     gene            41..391
                     /locus_tag="N1940_RS28975"
                     /old_locus_tag="N1940_28975"
     CDS             41..391
                     /locus_tag="N1940_RS28975"
                     /old_locus_tag="N1940_28975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(461..727)
                     /locus_tag="N1940_RS28980"
                     /old_locus_tag="N1940_28980"
     CDS             complement(461..727)
                     /locus_tag="N1940_RS28980"
                     /old_locus_tag="N1940_28980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(711..935)
                     /locus_tag="N1940_RS28985"
                     /old_locus_tag="N1940_28985"
     CDS             complement(711..935)
                     /locus_tag="N1940_RS28985"
                     /old_locus_tag="N1940_28985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(1602..1814)
                     /locus_tag="N1940_RS28990"
                     /old_locus_tag="N1940_28995"
     CDS             complement(1602..1814)
                     /locus_tag="N1940_RS28990"
                     /old_locus_tag="N1940_28995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1821..2330)
                     /locus_tag="N1940_RS28995"
                     /old_locus_tag="N1940_29000"
     CDS             complement(1821..2330)
                     /locus_tag="N1940_RS28995"
                     /old_locus_tag="N1940_29000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2365..3069)
                     /locus_tag="N1940_RS29145"
                     /pseudo=""
     CDS             complement(<2365..3069)
                     /locus_tag="N1940_RS29145"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3008..3352)
                     /locus_tag="N1940_RS29005"
                     /old_locus_tag="N1940_29010"
     CDS             complement(3008..3352)
                     /locus_tag="N1940_RS29005"
                     /old_locus_tag="N1940_29010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(3342..3518)
                     /locus_tag="N1940_RS29010"
                     /old_locus_tag="N1940_29015"
     CDS             complement(3342..3518)
                     /locus_tag="N1940_RS29010"
                     /old_locus_tag="N1940_29015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4158..4316
                     /locus_tag="N1940_RS29150"
     CDS             4158..4316
                     /locus_tag="N1940_RS29150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            4674..5000
                     /locus_tag="N1940_RS29015"
                     /old_locus_tag="N1940_29020"
     CDS             4674..5000
                     /locus_tag="N1940_RS29015"
                     /old_locus_tag="N1940_29020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            5026..5571
                     /locus_tag="N1940_RS29020"
                     /old_locus_tag="N1940_29025"
     CDS             5026..5571
                     /locus_tag="N1940_RS29020"
                     /old_locus_tag="N1940_29025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     Typing          complement(3605..3681)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          3870..4267
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(1487..3018)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agagaggata gtgaaatgtg taataccaag attctcaact atgtattggt taattgcgtt
       61 cctagcattt atattccccg gtcgatcttt tgctgacggt tacaccgctt actaccgatg
      121 taatggtact caggaagcat ctcttactgt taaagataac aaactaagta tggttgtagg
      181 tctttctaag tttaataggc aactggatac ttatagattc aaagaaaagg gaatcgaagt
      241 agtaatgcag agagctgctt ctgatgataa ctctgaggcc ataatgtctt ttattatgcc
      301 tgacgttaac aatatgacct tattagttaa aaataacggc tacattatcg ttaagacaac
      361 ctgctggtta actaaaaccg acaaacagta gaacacgtag ttagtcgagt atttaccata
      421 atacctgttg tcggcatcaa aaaagcccgc atagcgggct tcagtcgtaa acttcgcggc
      481 gatgtccaat cgtggcggca aggataacaa gctcgtcatc tcggatctca cacagaacgc
      541 gataatctcc cacccgatag cgccagaact cgcccagctc acctttgagc ggcttccctg
      601 actggcgagg atcggcagca actgcaatgc gtaaactcat aaaatccaca atccgtcgtg
      661 cgttctgttt gtccatcttg cgtaacgatt tgagcgcccg atcggaatag ttaatcgtcc
      721 agaccaagtg acttcctcac gtcttcagac gaatgcactg cttcatcacc acggcgaacg
      781 cgtgcagcag tttctgctga aagataataa tcctccaggt cttccagatg cgccagtatt
      841 gcttcccgcg cataaaacgt ctttgttctg ccggtttgct tcgccagcga gtccagacgg
      901 gactcaatct catcagataa ccggatagca agcatataaa cctcccgaac accatttgct
      961 atacatgtat atcaacagta ccacaatcaa cctaaaagaa caaaaaataa tcttgttagc
     1021 ccctcttgat aatccgtaac gcggtcaata tatctagctt gagggctttc ctcttaacta
     1081 ttgaaaggat aacgattatg ggtcttaagc cgggtccgaa acgaattgcc gattcaacag
     1141 gggaacctga taaacgccag cgtgacaata aaaaaacgcc gggaaatact gacaaattga
     1201 aacccagcaa atcttcaaaa aaataagtca gtagttaaaa gccctccggg gcttttttgt
     1261 atctgccgtt cacgctgatt aaacataaca cccgttgccc gcaccgcccc ggacgggttc
     1321 gccgcgaacg gggtggcagt caggtaaaac cggcaacaaa ccacccgata aaccgccccg
     1381 ttcgcgacag ataaatttta cctggctgca tagccatgca gccaggtgaa tgacatccgg
     1441 tttcagcgta aatgcccgat gagaaccctg ttttcctgca tttcagccca gcgacggacc
     1501 gtcatagctt cgctgccggg aacgcgcttc cgtcagttcc ttttcgtgct gccgttgctc
     1561 gtgcgcccgt tctgcggcca cggccatctc atgacgctgc actaccggct caaacgctgc
     1621 agttgcccgc tcaagcgttc cacctgctga ctcaagcgcg tcacgcgctc gctcagcgcc
     1681 gcgttctccc gttgcataag ccctgacatt ttccgccatt ccccgaaggc gctctcccac
     1741 tcgtccagcc ttttcgagta gtcctgctgt agctgctcta atgcgctcag caactgtttt
     1801 tccagctcgg tcatcggcac ttactccttc accagtctca gcgcgttctg cttcgaccgc
     1861 ccgttcccga ccgtccagct ctgcgtcctg tctacggccg tccccttcgg cagcaccaga
     1921 aaacgtccgt tgctgtcttc caggtacgtc acgccccagg tcttcgcgtt cagcttcgac
     1981 agcgctgcgt tctgccggtc gatttcggcc agatttgacg ctatcaagct cccctgatac
     2041 cacaacaccc cgctcaggct ggcgaacagc agtcctgaca ccatcgtgat ggtcagccat
     2101 gtccgcccga ccatacgcat cacgctcagg cggttcgact gcatggccgc gctcataccc
     2161 tgctcgtggg cgcggatggc ggcgctgatt ctcttcccgc tttcgctcag ttccgctctg
     2221 acagacttct cgtgctcgct gaatgcggct ttcagcattt cgccggtact ctgctgctgc
     2281 gctttcgatt tctgctctaa gtccttcgcc agcgttaaaa gactgttcat agatggctcc
     2341 cttaagtcgg atgtttcgcc ctccgtccgg gtcggcaatg ctgatgctgc tctttgtggt
     2401 gcgcacgacc tcaaaacctg cgctttccag cgcctcagtg acgtcctgac gcgttttaag
     2461 ctcccctgac gaggcgaggg ccagtaagcc ccgcgtaata gcctctgcgg cttcctgctt
     2521 cgttttgggt aacgaagatg gcgtcaccag cgcgcgccgg ttctccggtg cgttcgggtc
     2581 gtgcagcccc agtcgcccgt tcaccacggt ctgccacgca tcgatgcgcg gacggtctgc
     2641 ccggtcgtag tacggctgga gacgcctgcc ggtcagcagt tccgtgttcg gtaccagaaa
     2701 attcagttcc agccgcccct tgtcccggtg ttcaacccac agcacgctgt actggtcttt
     2761 atcgagtccg ggcatcagaa cccgctcgaa gctcgccatc agcttttcac gctggccggg
     2821 cggtaaatcc tgttcagcaa aagacaggac gccggaggtg tactttttgg cgtagggcga
     2881 ggcgtcgata agctcccgga cctcctccgg ctttccctgc aggacgatgg ccccgtcgcg
     2941 ctggcggtct ttccccagca gataatccac cggcccggca ccgccgcctc gccctcgcgg
     3001 atgaaactta acgatcatcg tctgccccct tttccagcac ggcatgccgc agccgctcga
     3061 gttcggcatc aatggccatc agcgcggcca ccacctgaac ccggtcagcg cctgaccact
     3121 ggccgccgtt aattttccgg gcgatctggt tgaggttgtt ccccatcccg gcaagctggc
     3181 gcagcaaaac cggatcgatg gacggcagcc gcaacgaccg cgccgggcgc gtatcgagac
     3241 aggtttcccg catccacacc gccagttgcc ttccattgca gcgctccagc agctgctggt
     3301 actccccatc ggtcacccgg atacctaaaa aacgggtacg cttaaccgcc atcctctcct
     3361 ccgatatccg caaaatgtga ccgggctgaa cgctcaagct ctgccatctg ctcccggcgg
     3421 taatcttcct cctgtgcctg acgcatcaga tttgccatca ctgccggatg ccaggatctt
     3481 ttttttacga cgagcggctt cttcttcggc ttctgcatta tccctccttc gcaaacgccg
     3541 gacggcacgg aaaagttttc cgggtcgctc cggcgttgga caagcgccag cgcgtcagtg
     3601 gtgttagcgg gtttcggggc gcagccctga accagtcaca gcgtttacgc tgtgtatact
     3661 ggcttactat gttggcatga gagcacgatt catgaaagtg cctcatgtgg caggtgtgat
     3721 atgtggctca ggagcgtccg caataccatg cacggacaga tgaaatgacg cctcctcgct
     3781 cactcggtag ctgcgctcct gccgttcggc tgcggcgggc gtgaccgact cacggataac
     3841 gggataaagc aaaggcgctc aaatcagaaa gggccggaga aaaggatgcg gcgttgccgt
     3901 ttttccatag gctccgcctc ccctgacgag catcacagaa atggacgctc aagtcagagg
     3961 aggacgaaac ccgacaggac ttaaagatgc cggtgatttc tctccggcgg ctccctcgtg
     4021 cgctctcctg ttccgaccct gccgtttacc ggatacctgt tccgcctttt tccctttcgg
     4081 gaagcgtggc gctttctcat agctcacgct gctggtatct cggctcggtt taggtcgttc
     4141 gctccaagct gggctgtatg cacgaacccc ccgttcagcc cgactgctgc gccctttccg
     4201 gtaactatca tcttgagtcc aacccggacg gacacgcaaa aacgccactg gcagcagcca
     4261 ttggtaactg gatatatacg agagagatat tgagaggtct tgaagtgggt gcctaactgc
     4321 ggctacactg aaagaacagt tttggtatct gcggtctcgt atacccagtt acccggttaa
     4381 gcagttcccc aactgactta accttcgatc aaaccgcctc cccaggcggt tttttcgttt
     4441 acagtgcaag agattacgcg cagaaaaaaa ggatctcaag aagatccttt aaatgatcgt
     4501 cagttctgtt atccccggag ctccatccat cccacggaaa aacacccgcc actgatgccc
     4561 tcagcgcact gcacatcatg tacaaaccac tccctccctc cttttttatt gcgtttctta
     4621 atgcgcaatg gtagctttgt gcgcaacaaa aaacgcaata gcaagggggg gtaatgagag
     4681 aaagaacaaa tatcatgcta gatataggat taaagcaatc cttacaacgg ctttcgaata
     4741 aaaccaaaat gagtctaagt gatcttatta accagagatt atcaggttca ctttcagttg
     4801 aagaacgcat tggcgaaaag cctgaatgga tgagaaacct tgatccctat ctcgccaatc
     4861 acttttcaaa tattgagtta ccgctccctt caccattaaa agaataccaa gaaaaaatgg
     4921 atgagataga ttcaattatt aatgacatga tggttctaaa ggaatatttc agagctaagt
     4981 tttacgacga aataaaatag gcacgctata cggaggcgga gaattatgtt ttttaaacgg
     5041 tatttaacgg agaaggaaat atacaaggca gcactcccaa ttgttaggct tcaagggaga
     5101 gttaacactt cattacgttt tttatccaaa tactcagatg taaacaaaaa tggattaatt
     5161 gcattgcatc ttaatttatt acattttatc tcatacaaca tacacggtgt agagtctctg
     5221 aaaatcactc aagcaggcat aaacaaagct gtacaacaaa gccctcacgc agatgaaata
     5281 aaaaatattg cgcaggcaat aggacgcaaa ggacctgagt acatcgaagg attaattaga
     5341 aacccatggg ggaagaaaaa tgataatcca ctaatattta taatgagcat tgaagaaagt
     5401 ataaaagaaa cccttaactc aggttatatg ttatatagag tagctcccat aactgagggt
     5461 gaagcagctg ctttcatgga tgttcttaaa caattattat tatttgagga taattttcgc
     5521 ctttttggta gagacttggt ttataaaata gaaaaattaa aaatcagata acaaagtatg
     5581 gtgaacgaaa aatgaa
//
