LOCUS       NZ_CP104033            10060 bp    DNA     circular CON 25-SEP-2023
DEFINITION  Klebsiella pneumoniae strain 150040X0B1 plasmid p1_150040X0B1,
            complete sequence.
ACCESSION   NZ_CP104033
VERSION     NZ_CP104033.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN30672525
            Assembly: GCF_025152765.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Yin,X., Liu,L., Feng,Y. and Zong,Z.
  TITLE     The complete genome of non-phage-resistant Klebsiella pneumoniae
            strain 150040X0B1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Yin,X., Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2022) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Trycycler v. 0.5.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/25/2023 03:15:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,823
            CDSs (total)                      :: 5,701
            Genes (coding)                    :: 5,512
            CDSs (with protein)               :: 5,512
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 189
            CDSs (without protein)            :: 189
            Pseudo Genes (ambiguous residues) :: 0 of 189
            Pseudo Genes (frameshifted)       :: 65 of 189
            Pseudo Genes (incomplete)         :: 135 of 189
            Pseudo Genes (internal stop)      :: 20 of 189
            Pseudo Genes (multiple problems)  :: 28 of 189
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP104033.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="150040X0B1"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p1_150040X0B1"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2020-06-12"
     gene            complement(join(9996..10060,1..307))
                     /gene="tolA"
                     /locus_tag="N1940_RS28910"
                     /old_locus_tag="N1940_28910"
     CDS             complement(join(9996..10060,1..307))
                     /gene="tolA"
                     /locus_tag="N1940_RS28910"
                     /old_locus_tag="N1940_28910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(581..826)
                     /locus_tag="N1940_RS28915"
                     /old_locus_tag="N1940_28915"
     CDS             complement(581..826)
                     /locus_tag="N1940_RS28915"
                     /old_locus_tag="N1940_28915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            1153..2838
                     /locus_tag="N1940_RS28920"
                     /old_locus_tag="N1940_28920"
     CDS             1153..2838
                     /locus_tag="N1940_RS28920"
                     /old_locus_tag="N1940_28920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            2848..3105
                     /locus_tag="N1940_RS28925"
                     /old_locus_tag="N1940_28925"
     CDS             2848..3105
                     /locus_tag="N1940_RS28925"
                     /old_locus_tag="N1940_28925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            3190..3339
                     /locus_tag="N1940_RS28930"
                     /old_locus_tag="N1940_28930"
     CDS             3190..3339
                     /locus_tag="N1940_RS28930"
                     /old_locus_tag="N1940_28930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            4823..6787
                     /locus_tag="N1940_RS28935"
                     /old_locus_tag="N1940_28935"
     CDS             4823..6787
                     /locus_tag="N1940_RS28935"
                     /old_locus_tag="N1940_28935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            6787..7518
                     /gene="mobC"
                     /locus_tag="N1940_RS28940"
                     /old_locus_tag="N1940_28940"
     CDS             6787..7518
                     /gene="mobC"
                     /locus_tag="N1940_RS28940"
                     /old_locus_tag="N1940_28940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            7525..8055
                     /locus_tag="N1940_RS28945"
                     /old_locus_tag="N1940_28945"
     CDS             7525..8055
                     /locus_tag="N1940_RS28945"
                     /old_locus_tag="N1940_28945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8082..8261)
                     /locus_tag="N1940_RS28950"
                     /old_locus_tag="N1940_28950"
     CDS             complement(8082..8261)
                     /locus_tag="N1940_RS28950"
                     /old_locus_tag="N1940_28950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8287..8715)
                     /locus_tag="N1940_RS28955"
                     /old_locus_tag="N1940_28955"
     CDS             complement(8287..8715)
                     /locus_tag="N1940_RS28955"
                     /old_locus_tag="N1940_28955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(8879..9193)
                     /locus_tag="N1940_RS28960"
                     /old_locus_tag="N1940_28960"
     CDS             complement(8879..9193)
                     /locus_tag="N1940_RS28960"
                     /old_locus_tag="N1940_28960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(9181..9459)
                     /locus_tag="N1940_RS28965"
                     /old_locus_tag="N1940_28965"
     CDS             complement(9181..9459)
                     /locus_tag="N1940_RS28965"
                     /old_locus_tag="N1940_28965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(9634..9999)
                     /locus_tag="N1940_RS28970"
                     /old_locus_tag="N1940_28970"
     CDS             complement(9634..9999)
                     /locus_tag="N1940_RS28970"
                     /old_locus_tag="N1940_28970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     BGC             1..7838
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(3443..4021)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6788..7515
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 gaattttggc gtgggtaacg gctgatattg ctgctttaca gagtttcgga tctcctcctt
       61 cagcaatggc gctgttcaga gtgccatcac gttgtatgga tatccgcacg gtgcacattt
      121 ttcccgcata ggcctctggt tcaaacatct gcatttcaac agcggtcttg atggccgcag
      181 cgtagctact gattttagat gacttatcca gagtataaat tttcggcggc tgggtggtgg
      241 aatgcttaca ggccgtcagg agtaaggggc tgcacaatgc cagcaggata aagggtatta
      301 aacgcatcgg gttgatgacc tgtcagtaaa aaatgttaca tgtatatttg tcactcttta
      361 cggccacgtt catttacata aacacggctt atcgtatcgt ttttacacca aacggcccgc
      421 catgcagttg cgtgcatagg catgcagcgg gttacaattt accgggcgcg tttccggcgg
      481 ttttccgggt ggtttgttgc ctgttttatc ccgtagccgc cggaaacgcc ctcagaccgt
      541 ctgagcggtg cgggcaatgc gacgttatgg taaatacccc ttaccggcgg cgctgtggct
      601 gccaggtgaa ggtggtttcc gtagcctcgt atttctcccg acgcatttcc tgcgcccgcc
      661 tggcttcacg ttcgtcctga atggcacgaa gcaccctttc ccgcacctgt tgttttaccg
      721 cctttggcag cgttttgttc cctttcaggc ccatggccag tgcctgagcg acaaaggcgt
      781 ccgccaggcg cttttcttca gcgggagtca gtttcaggtg cgtcatagtg gcctccgggc
      841 gtctcaggtt tcccgaacag tttaaccgca ggtcctctcc tcatgccagt tagctttcag
      901 atgtgtctat accatctgaa atgtaagtgt gatgaatgtc ttaaataata aaatttgttg
      961 ccatgagaaa ttttcgggaa aagacgcgat ctgcttacag gtggctccgg gcgctttttt
     1021 gtgcctgaag atgagaacta atctcatata ttgacacctg aaaacgggag gagtaaggta
     1081 ataattatac tgtgtatatg tacagtatgt tggctttaat ttattgtttt aaatgtcaaa
     1141 gaggaaaacg gtatgagtgg tggagacggt cgaggtccgg gtaattcagg tctgggacat
     1201 aatggtggtc aggccagtgg gaatgtgaac ggtacgtctg gtaaaggtgg cccttcatca
     1261 ggtggtggta cggatccaaa cagcgggccg ggctggggta cgacgcatac gccgaatgga
     1321 gatattcata actataatcc gggggagttt ggtaacggcg ggagtaaacc cggtggtaat
     1381 ggtggtaaca gcggcaatca tagcggtagc tctggtggcg gacagtcttc ggccactgcg
     1441 atggccttcg gtctgcctgc cctggctact ccgggggctg aaggactggc tttatcagtt
     1501 tccggcgatg cgttgtcggc cgccgttgct gatgtgctgg ctgccctgaa ggggccgttt
     1561 aagtttggtc tgtgggggat tgcgatctac ggtgtgctgc cttctgagat tgcaaaagat
     1621 gatccgaaaa tgatgtcaaa aattatgacg tcattaccgg ccgatgcggt gacggagact
     1681 ccggcaagta ctttaccact ggaccaggcg acggttcgtg tcagacaacg ggttgtggat
     1741 gtggtgaagg atgagcggca gcatattgcg gttgtcgcag gtcggccaat gagtgtccct
     1801 gtggtggatg cgaaaccgac aaaacgtccg ggggtattca gtgtgtcgat tccgggtctc
     1861 ccgtctctgc aggtgagcgt accgaaaggt gttccgacag cgaaagcccc gccaaaaggc
     1921 attgttgctg aaaaaggtga ttcacgtccg gctggtttta cagccggtgg taactcccgt
     1981 gaggccgtta ttcgtttccc gaaagagacc ggacagaagc cggtttatgt gtcggtgaca
     2041 gatgttctta ccccggcaca ggtaaaacag cgtcaggagg aagaaaagcg tcgccagcag
     2101 gcatgggacg ccgctcatcc ggaagagggg ctgaaaagag actatgataa agcgaaagct
     2161 gagctggatg ccgaagataa aaatattgcg accttaaaca gccgcattgc atcgacagag
     2221 aaggcgctcc ccggtgcaag ggctgctgtt caggaagccg ataaaaaggt aaaagaggca
     2281 gaggcaaata aagatgattt tgtgacttat aacccgcctc atgaatatgg ctccgggtgg
     2341 caggatcagg ttcgctatct tgataaggat attcagaatc agaatgagaa attaaaagcg
     2401 gctcaggcat ctttaaacgc aatgaatgaa tccttatcca gagataaggc tgcacttacc
     2461 ggggcgatgg agagccggaa acaaaaggag aaaaaagcga aggatgcaga aaataagtta
     2521 aatgaggaaa agaacaagcc tcgcaagggg actaaagact acggccatga ttatcatcca
     2581 gtacctaaaa cagaagacat aaagggactt ggagagttaa agcgaggtga tcctaaaaca
     2641 cctaaacaag gtggtggtgg taagcgggcc cgatggtatg gcgataaaaa acgtaaaatt
     2701 tatgaatggg attcccagca cggtgagctt gaagggtatc gtgccagtga tggcgaacac
     2761 ctaggcgcat ttgatcccaa aacgggtaag caggttaaag ggccagatcc aaaacggaac
     2821 attaaaaaat atctttgaga ggtaaatatg gggcttaaat tacgtttgac atggtttgat
     2881 aagaaaactg aagagtttaa aggggaagag tattctaaag actttggtga cgatggttct
     2941 gtcattgaaa gcttgggaat gcctttaaag gataatatta ataatggtga gtttgatttg
     3001 aagaaagaat gggttccttt actacagcca ttttttaaaa atcaaataaa acctgatgtg
     3061 gataattgtt atatagcttt tgattatcgg gacggtaact ggtaacaaaa aattatcaag
     3121 gatgatttga aatactgact tccggtgttc agggatgaat gccggagtct ttaaatacag
     3181 ggaaagtata tgaaaaaggt aaagactatt tttcttttta tattgattgt tgctggcttt
     3241 cttctggccg catgtcaggc aaattatatc cgggatgttc agggcggaac ggtggcacca
     3301 tcgtcctcct ctgaactgac ggggatcgcg gttcagtaga aaagatcaaa ggatcttctt
     3361 gagatccttt ttttctgcgc gtaatctttt gccctgtaaa cgaaaaaacc acctggggag
     3421 gtggtttgat cgaaggttaa gtcagttggg gaactgctta accgtggtaa ctggctttcg
     3481 cagagcacag caaccaaatc tgtccttcca gtgtagccgg actttggcgc acacttcaag
     3541 agcaaccgcg tgtttagcta aacaaatcct ctgcgaactc ccagttacca atggctgctg
     3601 ccagtggcgt tttgcgtgct ttcccgggtt ggactcaagt gaacagttac cggataaggc
     3661 gcagcagtcg ggctgaacgg ggagttcttg cttacagccc agcttggagc gaacgaccta
     3721 caccgagccg agataccagt gtgtgagcta tgagaaagcg ccacacttcc cgcaagggag
     3781 aacggcggac aggtatccgg taaacggcag ggtcggaaca ggagagcgca agagggagcg
     3841 acctaccgga aacggtgggg atctttaagt cctgtcgggt ttcgcccgta ctgtcagatt
     3901 catggttgag cctcacggct cccacagatg caccgaaaaa gcgtctgttt atgtgaactc
     3961 tggcaggagg gcggagccta tggaaaaacg ccaccggcgc ggccctgccg ttttgcctca
     4021 catgttagtc ccctgcttat ccacggaatc tgtgggtaac tttgtatgtg tccgcagcgc
     4081 ccgccgcagt ctcacgcccg gagcgtagcg accgagtgag cgaggaggcg caatttcccc
     4141 ggcacctgca agacggtcac cggacgcgct gatgtgggat attgtcccct gattaagtct
     4201 ttctattccc tgacgatcaa ttatcaggat aggtgaacca tgtcggtttt tcgctgaaca
     4261 ttttacgtta acctatctct gttgcttttt tctccttgcg gagaaatgca agtcatcccc
     4321 gctggggcgt ggctgacttc aggggaaaag accttgctta cgctggctgg acgccaccga
     4381 cgcacggtct tttcccctct ttctggctct gtccggctgg acgccggcca cgccagaaat
     4441 tcacccggcc cccgtcaggc tcacgcctga ccacccacaa aaatccacgg tgctggacgc
     4501 acctgacgaa gcagcaaagc tgctcctgat tttgcgggtg gtcagccgct cgctatggcg
     4561 aactctgacg ggggccggaa caccggaaaa gaccgcactg gggtgccgcc ttttcctctc
     4621 acacgctggg gcgtgctcgt tgttcctccg gggctgcgcc ttttccgcat ggggcggaaa
     4681 gaggctggcc tccgcggtga aagctgaacg caatcaccac ggacgtgacc gccgccgcca
     4741 tgccttgggg catgtccgcg gcttgtgcag cgggggctgc gcatcacgcc ggtaaacccg
     4801 tcgtctgaag gagagagatt gcatgtttaa tacggattcg cttgcctggc agtggcatat
     4861 cccggagctg gcccggcaga tttacccgtg ggtcagctgg ctgtaccggg agtattacag
     4921 tctggccggt ttttttctgg ttatcggcct gtttttcggt ctggctgaac tctgggccgg
     4981 ccgaatgcgt tttgtggggc tgagccataa ggcgcggcgg gagtatgccg atcgctataa
     5041 gcgcgataat aaccggactg tgccattttc ggtgctggcc tcgctggatt cacccgggcg
     5101 gatggtcagg gcacggcgac atgtctggaa ctacgcgtac tggctgaccg gcgctgccgg
     5161 tctggccctt ctgggggggc tggttgtcgc cgtgtttatc gagagctggg gagaaaccgt
     5221 cctgcgcccg gacctctatg cgaacgcaga ttattacctg ctgcagccgg ctaacctgtt
     5281 cgggatagcc tggtgtgccg gagggcttgt cgccggcgtc cttctggcgc ggctgctggc
     5341 gttcctgctg cttgatggtc attttgcggc cgctgacgag gcggtgaacg cgaaactgaa
     5401 tcaggagagc cggcgcagca gccagcggac cggagaaatg acagacgtcc gccacctgca
     5461 tttcggggag ccggtaccgg ttaacgcgct ggccgatttt tccacggagc aggcccggaa
     5521 acagcaggcg gtgtttctgg gtaaggatga gcagggccag ccggtgctgg tccctcgtga
     5581 tacctggcgc aagaccaata ttcagatcct cggcctgccc gggagcggca agagcgcgat
     5641 ggccaccaac gcgctcattc gctgcgtacg cgatttcggg gacgctgtgg tgtacttcga
     5701 ccccaaaggg gacgcgtggg cgccgcacgt cttccgggcg cactgcccgg acttcaccct
     5761 gctggatctg cggccgggga aaccggcgca gctgaatctg ttccgcgatc tcgaccagta
     5821 cgccctgaag aatctgctgg ttgccgggtt taacctgagt gagaccggcg acgtggcgga
     5881 ccactaccgc atcagcgagc agaaagcggc gaagctgatt gcggagcagt tcccgcaggg
     5941 ggcaaacatt cagcaggtgc tggcggcggc gtacgcgttg ccggaggccc tgaaaaagga
     6001 cgtgaagggg ctgatcacca agctggagaa cgtggccgac ctgagcgtcc tgcagacgga
     6061 cagcggtatc gatgtggcgg ggataatcaa cggcggcggc tgcctgtacg tcatcggctc
     6121 gatggacgat gaggcggtga tccgggtgca gaagatgctg tttgcccgct gcgcgcaaat
     6181 catcattgcg cgggatgagt tccggaggtg gccgcacgcg agcatcatgc tcgatgagat
     6241 taaatatctg ctgtcgaagt acgtgctgaa cgcgctgggg acgctgcgca gccgggactg
     6301 caacctgctg ctcgcgcacc agtcgctggg cgacttcggt cagtgcgggc aggatctgcc
     6361 cgctgatttt gtgaaaacca cggtactgga taacacgccg atccgctggt tctatcgcgc
     6421 ggccagccag gagtccgcgc agtgggcggc gggccagacc ggggagatcc gggtggacgt
     6481 ggagcgccgc cgggccagcc gggaggcggg gaacgtggag cacatcagcg gcgacagctt
     6541 catccagaag gaggcgcggc cgctgtttga cgtgaacacc ctgcaacacc tgcctgacgg
     6601 gttcgcggtg atgaccgggc tgggcgtggc gcggctggcg ttcagcagcc cgctgcgggt
     6661 tgagcgccgg gaaatttcgc tgaaaacctt cccggtgctg gcgaaaacgg acccgctggc
     6721 ggagtacctg cctccggccg acggcggcgg accaaaagcg gatgatgatt ttgcggggct
     6781 ttactgatga cactggagca gtacaacgtc agccacgcga agcggcaggc gcggaacgcg
     6841 gagaaaacac ggctgaccct gcgctggctg cgggaagagc tgtgttccac ggcggagctg
     6901 gttgcgcgac ggctggggat tgcggcggta cagccggttt accgcttcct ggacagtctg
     6961 gtggcgaaag ggctgctggt ccgggcgaaa tacccggttg acggccgtca ggtcagcgtg
     7021 tgggggctga cgccgcacgg ggtggcgttc tcgttcgacg aggacgagcc gctgacggac
     7081 gttattccgt ttcagccgtc aagggtgtcg gcggcgcagc tgccgcaccg gctggccgtg
     7141 cagtccctgc gcctctccat ggagggccgg ggggccaccg gctggcggta cctgcaccgt
     7201 atggccctga aagggatgaa agtgccggac gcgctggcgg agctggacgg cagaacggtg
     7261 gcatttgagg tagagcgcac cgtcaagagc cgcaggcggt atcaggaagt ggtgtccggg
     7321 tatctgttca accgccgggc gaacggcatc gatgaaatct ggtacatctg ccctgaccgg
     7381 ccgacgcttg tccgggtaca gcgggcgatt ctggcggtgg atgaaatcgt gaatccacag
     7441 accggcgagg cccgcaaaac ggtggagctg gaccgggaga ggctgttcgc gtgctttaaa
     7501 tttatgacaa cggagtgagg gattatggcg ctgtacatgg tatttattga ggacgtggaa
     7561 gacaaagacg gcgagcgctg cattatgcca accagcgagc atctggaaga catggtggtg
     7621 ctcgcctggc agaccggggc gtatgttttc tgttttgtcg accgggccca gcatctggcg
     7681 atgatgaaat ccatcgggat gtatgacgag cggctcaaca gcgacgaaaa agccacgtac
     7741 tttttcaccg gtgaaaatcc gcaatgggaa cgggacaaac ccacaggaat gaagctgtgt
     7801 gattacctct atcgaaaggt ggagcggttc gccatcgaga acggtgtgca ggtggttgat
     7861 gccgggaagt gctcaccgga gcagattaac gcgatgattg aggtggtcag cgagcggcac
     7921 ggggaaagcg cggtgaacga gaagctgaaa gcggagctga tgcgggaata taacagccgc
     7981 tattttaacg tgccggtgcc tgaactgccg gaggacaaac ccgcccgccg gtggtggccg
     8041 ttcggcaaaa aataacgcca gcaccggcat ggccggtgct gttattcttc cccgagctta
     8101 tcccgcatca cctgccagag tgtttccgca tcctcgtgaa gccgttcgca catgtcggcc
     8161 ggggtatcca tgtccagctc gttcagcttt tccagcaaca gcagcgactg atccttaatg
     8221 aatttagcca tattgagggc agttttttcc tgcttcgtca tgttctccat tcccttcggt
     8281 aagttctcag ctaaaccagc cggaccagaa gcgttttttc ttctgtgtca gttctgttgt
     8341 caatctgtcc acttcggcct ggagtttccg gcgctcatca tcgtgctggc ggcgttcttc
     8401 ttcccgggat gttttatctt ccagcatcag ggtgacagcc tgccggagat cggccagttc
     8461 cttttgcatt gccagaagct gttcctgtgg gaaatttttt tgtgtatttt cacggtgtga
     8521 aatgccggga atttgtttct cagggatatt cacatttccg tatacacgga tcagttctga
     8581 cacatccacg acagggttgt ttttcccgtc acgtgacacg gtaacctttc cctgttttac
     8641 atgattgtat agcgttcttc tggtaatgcc cgcggcttgt gctgcctgtg aaaggttgag
     8701 caatgttttc gccactgtat acccctgatt ttatcgggtg tgaaaggtgt gtaatttctc
     8761 acggtaaatg tatcgctgaa ttttacccgt ttttccgttc aggatgtggt tttctgacgc
     8821 gctgcgcttg tcaaaccggc gacggccacg caatgaattg cgcccccgtc atcggttatt
     8881 agaggctgtc tgcccccgga tacccggatt ctttctgatg cctgacggtg acgatcacaa
     8941 tctggtccat ttcccggtcg tggcggtaga gcatcacata gccactgtcg ccaaacccga
     9001 tcaccagttc cttatattcc agcggcagaa acggcaccgg gcggccaatg tctggcaatg
     9061 ttttcagttg ctggatagcc cggacgatca cctcacctcc ttttctggct gccagccgat
     9121 ttttggtttt gagaaagtcc tgaaggcgtt gtaaatcgcg ctgtgccagc gcggaaatcg
     9181 ttacctgtgg catggcggag catcctgctc gttctcagtt ccccaggtac ttatccaggc
     9241 ttccgcttct tccgcagtaa ggtgcagacc cgtttcctga tagtgctgcc atgcggcctg
     9301 tccgtcccgc aggtactggc tgcgcttctc ttcccggtcg atgtattcgg tgattgcctc
     9361 cagcattagc gcgtgcgctg agcggtgccg gtcatcggcc agcgttttca gccggtcctt
     9421 aagttcttca tcgagcctga tggatgttgc tgatgacata aaagcccctt tgtagtcata
     9481 tgtattacac atgactactt taatccgtgg cggcgctatt tgccagaaat acggcttttg
     9541 ctgcccattt ttcagcttcg cttccactaa tcagggctgc tacaggctca ctcatcatcc
     9601 aggactattg cagcctgcgg gttaaaggac ctgttactgg cggcaaccat catcattgag
     9661 cttttgggtg gtgactggtc gcggatcctg taagccgtca tttttcagac tgaattcaaa
     9721 atttataacc tgtcgcattg ccgggtgccc tgcgggatat agccagtgcc tcattgccgt
     9781 cagcgtttcc cgaaaaaaaa ggtgacagcc ggtaagcgac acgttgtcca ctcgtccatc
     9841 gtccagaata tcaaacgtgg cggtcacctg accttcgatg cgttttgctg cggcctgtac
     9901 cgggtaggtt ggctgcagtg tggttacggg ttttagtggt atcgcatgta ccggagtgag
     9961 gaacatggcc gccagcactg tcagagtaaa agctttcatg gtctgaaatc cagaggggcg
    10021 tttttgaata tttgccaggt attttcatcc ggtgccggcg
//
