LOCUS       NZ_CP103766             8828 bp    DNA     circular CON 20-SEP-2023
DEFINITION  Escherichia coli strain 5283 plasmid pMB9880_4, complete sequence.
ACCESSION   NZ_CP103766
VERSION     NZ_CP103766.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28174091
            Assembly: GCF_024917655.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8828)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 8828)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 242x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/20/2023 06:28:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,193
            Genes (coding)                    :: 4,974
            CDSs (with protein)               :: 4,974
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 66 of 219
            Pseudo Genes (incomplete)         :: 160 of 219
            Pseudo Genes (internal stop)      :: 37 of 219
            Pseudo Genes (multiple problems)  :: 42 of 219
            Pseudo Genes (short protein)      :: 4 of 219
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103766.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="5283"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMB9880_4"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2020-02-08"
                     /collected_by="MDACC"
                     /genotype="ST405"
     gene            1..2484
                     /locus_tag="M5T11_RS26445"
                     /old_locus_tag="M5T11_26445"
     CDS             1..2484
                     /locus_tag="M5T11_RS26445"
                     /old_locus_tag="M5T11_26445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934115.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA transfer
                     family protein"
                     /protein_id="WP_220384729.1"
                     /translation="MKKNILAGIFFVAMLLPVWHAAAETFTRCHPNETVWAMIQDEQLK
                     NIARLQETKRCQAVNDTYDQWRQNFQAMGDAENAIRSARDHQNWNAVKSTFDNIAPQIN
                     SLHALAEKNSSLLGAGNILALFGGDLNYLYQNAGLVAPASADGLTDNVSQMLTEPDKQV
                     KAAGIAQQLVYQTRVRAQSFIQGVAQNAGNEAQKEYRAQSDKIVEDKAHNFKGDSVEGY
                     FGGFLARISQVYAPVLLFLIIVGIIGALTARRVRLDAFTASVKAVMAAAVPAVALALML
                     IFMPFLPSWVLYAAALAVTFAIYSRSKKILGGLASRCGRFPALASGLRWMGGFVSGLDA
                     DRKQETGAASVNPASHHTHGSSRWGTAAEMRENNHLLPTEKQAGFTLARELSPPPGVDG
                     KFCFTGHVVTVAPTGSGKGIGAVIPNLLTYQGSALVFDPKGENAEVTGRRRREMGQSVH
                     VIDPFGITSGIKESVNILDRLDVTSPDCVAESAVLAESLVVSEKGGDGNHFDESARTLL
                     QGLMLHVSGLPDESRRNLAELRRLITSDEENLLALLADMAASEDVAFGIPARAANTLMG
                     MADRERGSVLSTARKNTAFLDDPRIAAALSHSDFNLSAIKAEPMTVYLVLPANRIAANT
                     RFVRLFIGSVISAVTESKAKPAHNVAFILDEFNVLGYMKSIEDAVSLLRGYGLSFWVFL
                     QDLSQLKSAYPKWQTFLANSAKTFYGTDDYDTAKYVSDTLGKSTVEFETQNSGRNSGGG
                     VSGGGGSMNRGRSSGSSQQFAGRELLTPDEVMRLGPTRPIVILKGEYPYQLTRINYLTD
                     PEYTGMADPNPYYSA"
                     /name="type IV secretory system conjugative DNA transfer
                     family protein"
     gene            complement(2737..3099)
                     /locus_tag="M5T11_RS26450"
                     /old_locus_tag="M5T11_26450"
     CDS             complement(2737..3099)
                     /locus_tag="M5T11_RS26450"
                     /old_locus_tag="M5T11_26450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019105480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045889489.1"
                     /translation="MAWHSVSQAQNMTGKSRRTLYRDMASGRLSWRTNTSGHRELETSE
                     LIRVYGEIKNDGTAERHTVSQPDVTVSGSDIRDELRALRQEVADLKSMLLRLEHKQEEQ
                     PETAAPWWRFWQKKST"
                     /name="hypothetical protein"
     gene            3459..3599
                     /locus_tag="M5T11_RS26455"
                     /old_locus_tag="M5T11_26455"
     CDS             3459..3599
                     /locus_tag="M5T11_RS26455"
                     /old_locus_tag="M5T11_26455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_062939423.1"
                     /translation="MQEPPVQPDCCALPGNYRLESNPGRHDKAPLAAAIGNWEFAENLF
                     S"
                     /name="hypothetical protein"
     gene            complement(3987..4481)
                     /locus_tag="M5T11_RS26460"
                     /old_locus_tag="M5T11_26460"
     CDS             complement(3987..4481)
                     /locus_tag="M5T11_RS26460"
                     /old_locus_tag="M5T11_26460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_182945396.1"
                     /translation="MAGTVWNDVNTGREVFSMTHLHPQLHKVEVSGVQAEIEISFGFHV
                     FTDQKQVGKVMSFRGETRHFCQERYEGSKTIVQRILSAIENGEYITAFISKGQGQRYYH
                     LNHHDDFILMEIRKPEGKDNFLRIHVVTAYTLDEWGTVNKGRNLRFRYVLEQRLQGKKI
                     I"
                     /name="hypothetical protein"
     gene            complement(4485..5003)
                     /locus_tag="M5T11_RS26465"
                     /old_locus_tag="M5T11_26465"
     CDS             complement(4485..5003)
                     /locus_tag="M5T11_RS26465"
                     /old_locus_tag="M5T11_26465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045889486.1"
                     /translation="MSVRAQRAGKRNQSERRIELINTLWEGEEIETWDRNDRPRNDGFI
                     TVPRYLPLLGVLMDELSKGSPLSSTYLALWFRVSDEGLIEIRDKTVLALESGFASGRGV
                     TTWTGRMRKLKELGFISCREGSSGEFHNVLIVHPLVAVKKLLDEGKITKGKTYNTFAER
                     VIEVKSSWE"
                     /name="hypothetical protein"
     gene            5420..5773
                     /locus_tag="M5T11_RS26470"
                     /old_locus_tag="M5T11_26470"
     CDS             5420..5773
                     /locus_tag="M5T11_RS26470"
                     /old_locus_tag="M5T11_26470"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227664254.1"
                     /translation="MITQLKGRKIALVSFCWYELICTQMNGNQFSQEKKVKGSKPSIWS
                     ITEGEVIMATINLSLLEHDCGEAAREALLSGVPVYYADETTPENCVIKEYPDGRRELVS
                     FMTGMEKLVGANT"
                     /name="hypothetical protein"
     gene            complement(5934..6353)
                     /locus_tag="M5T11_RS26475"
                     /old_locus_tag="M5T11_26475"
     CDS             complement(5934..6353)
                     /locus_tag="M5T11_RS26475"
                     /old_locus_tag="M5T11_26475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063058.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_182945395.1"
                     /translation="MSEEQRRRGFMKAEASLRLEGIDPSGSPRYESVKARIISGEITYD
                     QGRAEILAFHTGKRAEPTTAGEMLAEEFLKPLNMSQGELAQATGIPEHHIDDIISNRRR
                     LSAEESYKLAEQFQTDKDFWLNLQALHDAWLTHQK"
                     /name="HigA family addiction module antitoxin"
     gene            complement(6496..8319)
                     /gene="mobQ"
                     /locus_tag="M5T11_RS26480"
                     /old_locus_tag="M5T11_26480"
     CDS             complement(6496..8319)
                     /gene="mobQ"
                     /locus_tag="M5T11_RS26480"
                     /old_locus_tag="M5T11_26480"
                     /inference="COORDINATES: protein motif:HMM:NF015354.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_072052381.1"
                     /translation="MAIFHLSVKTISRSAGRSATAAIAYRTGSRIVDERTGIIHDYTRK
                     SGVEHSEIFLPQNSPDWASNRETLWNAVEQKENRRNSTVAREFELAFPAELDGKQRLEL
                     LRDFCSSLVERHGMAVDAAIHAPGKEGDQRNHHAHVLCSTRRLSEEGFKDKTRELDDFK
                     SGEVNHWRSAWSEMTNRHLEKAGRSERIDHRSLEAQRESAISQGDSAKAAKLDRVPTIH
                     IGYAAAQMEKRGLETERGNEYRQIILRNAEIQPLSRAIQLIDNWRATHDNNSRAGHGSI
                     APVPERDKGRDKRGVRTAQSYTGRVGAVPPPSARGRLRNLSELDMVRFGERSEMLLPGN
                     VSRDVEHQRTDRNQPLRRENTVTSKSKRPEVIRLEWQSLRRDKLTFVQDKAGRIAARGA
                     AHVERQRQKISSHERQRPEQPSGLIGMFKRSAYEQAASTWQTIKNGLDRRLVQLKNRLG
                     FVRDYMRKSGAYEMSTPGERLADQLAAKENPILAGQIKSLNAQKNAEDMAAYRTRVQSR
                     KQEKADMKLETIEELKQRGDARRDKADKAERAELEKASHQASLQNETLSEETKELESGE
                     KEKLSKRDQVLEKFKAKVERDKQNERGGRGR"
                     /name="mobQ"
     gene            8479..8781
                     /locus_tag="M5T11_RS26485"
                     /old_locus_tag="M5T11_26485"
     CDS             8479..8781
                     /locus_tag="M5T11_RS26485"
                     /old_locus_tag="M5T11_26485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045889494.1"
                     /translation="MAKSRAEKLEELRQKEAQIKAQIQQLKQREQTEERKKDTRRKILI
                     GGAVLAKIKRGEWNYQQLVSLLDNELKNDRDRALFDEMGLKAVDARKESFNPGNK"
                     /name="hypothetical protein"
     Typing          3201..3568
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.566"
                     /coverage="85.0"
                     /evalue="8.49e-135"
ORIGIN
        1 gtgaaaaaaa atatactagc cgggattttc ttcgtcgcaa tgctattgcc agtatggcac
       61 gcagcggccg aaacattcac ccgctgccat ccaaatgaga cagtctgggc aatgatccaa
      121 gatgagcagc taaaaaacat cgccagacta caggaaacaa agcggtgtca ggccgttaat
      181 gacacttatg accagtggcg ccaaaatttc caggcgatgg gagatgcaga aaacgccatc
      241 cgcagcgccc gcgatcatca gaactggaac gccgtaaaat ccacgttcga taacattgct
      301 ccccagatta atagcctgca tgcactggca gaaaaaaatt catcgttact gggcgcgggt
      361 aacatcctgg cgcttttcgg cggtgatctg aattacctgt accagaatgc agggctcgtt
      421 gctccggcca gcgctgacgg actgactgat aacgtcagtc aaatgctgac tgaaccggat
      481 aaacaggtca aagctgcagg tatcgcgcaa cagctggttt atcagactcg ggttcgggca
      541 cagagcttta tccaaggcgt ggcacagaac gccggtaacg aagcccagaa agagtatcgt
      601 gcgcaatcag ataagattgt cgaagataaa gcccataatt tcaaaggtga cagcgtggaa
      661 ggatatttcg gcggcttcct tgcccggata agccaggtat atgctccagt tctgctgttc
      721 cttatcatcg ttggtattat tggtgcactt accgcacggc gggtaaggct ggatgctttc
      781 acggcatcag tgaaagcagt catggccgca gcggtacccg cagtggcact ggcgctgatg
      841 ctgatattca tgcctttcct accatcatgg gtattgtacg cggcagcgct ggccgtaaca
      901 ttcgccattt attcccgcag caaaaaaatt cttggagggc tggcctctcg ctgtgggcgt
      961 ttccccgcac tggcctccgg gcttcgctgg atgggcggtt ttgtgtctgg cctcgatgct
     1021 gacagaaagc aggaaaccgg cgcggcatcc gtcaacccgg catcacatca cactcacggc
     1081 tcatcacgct ggggaaccgc agcagaaatg cgggaaaata accacctgtt accaacggaa
     1141 aagcaagccg gattcaccct ggcacgagaa ctgtccccac cgcccggagt ggatggaaaa
     1201 ttctgtttca ccgggcacgt tgtgactgtt gcccctacgg gcagcggaaa agggattggc
     1261 gcagtaattc ccaatcttct gacttatcag ggttcagcac ttgtctttga tccaaaaggg
     1321 gaaaacgccg aggtgacagg tcgtcgccgt cgtgaaatgg gtcagagcgt ccatgtaata
     1381 gacccattcg gtataaccag cggcataaaa gaatcagtga acattctgga tcgtctcgat
     1441 gtgaccagcc cggattgcgt cgctgaatct gcagttctgg ccgaaagcct tgtggtatca
     1501 gagaaaggcg gtgacggtaa tcattttgat gaatcagccc gaaccctgtt acagggcctg
     1561 atgctgcatg tttccggatt accggatgag tcccgacgta atctcgcgga actacgtcga
     1621 ctgatcacca gtgatgaaga aaacctgtta gccctgctgg ccgatatggc cgcaagtgaa
     1681 gacgttgctt tcggcatccc cgcccgagcg gccaacacgc tcatggggat ggcagaccgg
     1741 gaacgtggct cagtattgtc taccgcccgt aaaaacacag catttctgga cgacccgcgt
     1801 atagcagctg cgctgtcaca ctcagacttc aacctgtcag ccatcaaagc tgaaccgatg
     1861 acagtttatc ttgtgctacc agctaatcgc attgcagcaa atacccgatt tgtaaggctg
     1921 tttatcggtt ctgtgatctc cgctgtaaca gaatcgaagg cgaaaccagc tcataatgtc
     1981 gcattcatcc tcgatgagtt caacgtgctg ggctatatga aatcaatcga ggatgccgta
     2041 agcctgttac gtggctacgg gctgtctttc tgggtcttcc tgcaggatct gtcacagctg
     2101 aaaagtgcct accctaaatg gcagacgttt ctggccaact cagcgaagac gttctacggc
     2161 acagacgact atgacactgc aaaatacgtc agcgacacct tgggtaaatc gacggttgaa
     2221 tttgaaaccc agaattcagg ccgcaactct ggcggcggag tttccggggg tggcggctca
     2281 atgaatcgtg gtcgttcatc tggctcaagt cagcagtttg ctgggaggga attactgaca
     2341 cctgacgaag taatgaggct tggaccaacc cgaccaattg tgattctgaa aggcgaatac
     2401 ccttatcagc tcacacgtat taactatctg acagacccag aatacacggg tatggcagac
     2461 cctaacccct actacagcgc atgatatttg gaatcgttgt cggcgttctg ttagctctga
     2521 tgtttggccg ggtcttgctc ccggccttaa ccgccgtcgc ctttgtgata ggcataccga
     2581 tatacttctc tttccgcttt gtctgtgcct ttatctgggc tctggtgacg caaaacagaa
     2641 gacacagtgc tatgccaatc gaggcacaaa agaccaccaa gcctgaaaca ctaccggata
     2701 acgtaatcaa ctttagccgc gaaaaagaga aaagggttaa gtagattttt tctgccagaa
     2761 acgccaccac ggagccgcag tttcaggctg ctcttcctgt ttatgttcca gcctcaaaag
     2821 catagattta aggtctgcaa cttcctgccg taaggcacgc aattcatccc tgatgtcact
     2881 accggatact gtgacatcag gctgtgacac ggtgtgccgt tcagctgtgc catcgttttt
     2941 aatctcgcca taaacacgga tcagttctga ggtctcaagt tcgcgatggc cgctagtatt
     3001 agttcgccat gacaagcggc cagacgccat atcacgataa agggtcctgc gacttttccc
     3061 cgtcatgttt tgtgcttgtg acacactgtg ccatgccata acaccacctt gattaaatga
     3121 cacactctac gggctgcact ctagcaggct aacggcatcc aggcacgcgg taagaaagca
     3181 aagagaaaaa cgccagaccc gtttttccat aggctccgcc cccctggcga gcatcacaaa
     3241 aacagacgct caagccagag gcggcgaaac ctgacaggac tataaagatc atcaccgttt
     3301 ccggtgaatc gctccctctt gcgctctcct gttccgaccg tgtcgcttta ccggatacct
     3361 gaccgccttt ttcccttacg ggacatgtgg cgctttctca tagctcacac tgtaggtatc
     3421 tcagctcgat gtaggtcgtt cgctccaagc tgggctgtat gcaagaaccc cccgttcagc
     3481 ccgactgctg cgccttgccc ggtaactatc gtcttgagtc caacccggga agacacgaca
     3541 aagcgccact ggcagcagcc attggtaact gggagttcgc agagaatttg tttagctaaa
     3601 cacgcggttg ctcttgaagt gtgcgccaaa gtacggctac actggaagga cagatttggt
     3661 tgctgtgctc tgcgaaagcc agttaccacg gttcagaaac ttgccggttt actgaacctt
     3721 cgaaaaacca ccttgccggg tggttttttc gtttactgga caagagacta cgacgataaa
     3781 caaaggatct caagaagatc atgacaaacc cgtgaagttg acaagtggca ttggtaagtg
     3841 gacaatatac ctgtcagcac aaaccggaac cttgaaagag gggtgctgat gccgggggca
     3901 accctcctag ccagtgagcg ggataacgaa aggccagacc gaaatttttg cagtaaaaca
     3961 aaggcgcctt gggcgctttt tgttttttat atgattttct tcccctgtaa cctctgctcc
     4021 aaaacatacc tgaacctcaa attccgtcct ttgttgaccg tcccccattc atccagcgta
     4081 tatgccgtaa caacatgtat gcgaagaaaa ttatctttac cttcaggctt tcttatctcc
     4141 attagaatga agtcatcatg atggttaagg tggtaatacc gctgcccttg ccccttggaa
     4201 ataaaggccg ttatgtactc tccgttttct atcgctgaca gtatccgctg cacgatagtc
     4261 ttacttcctt cgtatctttc ctggcagaaa tgccgagttt cacctctgaa actcattacc
     4321 tttccgactt gcttttggtc agtaaataca tgaaacccga agctaatctc aatctcagcc
     4381 tgcaccccag aaacctcaac cttatgcagc tgagggtgca ggtgtgtcat cgagaaaact
     4441 tcacgccctg tattaacatc attccatacc gtacctgcca tttcttactc ccatgaggac
     4501 ttcacctcga tcacccgttc agcaaaagtg ttataggttt tccctttcgt tattttccct
     4561 tcatccagta gtttctttac cgcaaccagc ggatgaacaa tcaacacgtt gtggaactca
     4621 ccggagctac cttcccggca tgatatgaat cccagctctt ttaatttccg cattctcccc
     4681 gtccatgttg ttacccccct tccactggca aagcctgatt ctaaagccag cacagtctta
     4741 tcccttatct caatcaggcc ttcgtctgaa accctgaacc acaacgctag gtaggtactt
     4801 gaaagtgggc ttccctttga taactcatcc ataagaacac ctagtaatgg aagataacga
     4861 ggtactgtta tgaagccatc gtttcgaggt cggtcattac gatcccatgt ttcaatttcc
     4921 tccccttccc ataatgtatt tatcagttcg attcttcttt cagactggtt acgtttccct
     4981 gctctttgtg ccctgactga catactcgcc tcctgttaat gcatgagagc caatttacca
     5041 aaaaatcctt ataaatcaat aacttttata aattccctcg gacaattact tccagttttt
     5101 atttttttta accataaaaa atgcaatatt tattacataa acggcagtta taagtaatat
     5161 atattacaaa aagaattcgc attaacatga tttaaaaaga gtttttattt aattgcggtg
     5221 ctccatgtgc tcaccgtgct ccatgtgctt ccggtgctcc cttgtgctcc atggggttgt
     5281 agggggcaag gaggggtgga gaaatcgaaa aataaattaa gagtcaccat gccggtcact
     5341 cgtatttacc ataacatccg ttagcagaac cgcctcagag gccctcagcg cgtttttatg
     5401 ccctaagcct caaaagacca tgattaccca attaaaaggc cgtaaaatcg cactggtgag
     5461 tttttgttgg tatgaattga tatgcacaca aatgaatggc aatcaattta gccaagaaaa
     5521 gaaggtcaaa gggtcaaaac catccatttg gagtattacc gaaggagaag tcatcatggc
     5581 gaccattaat ctatcactac tggaacatga ctgcggcgaa gccgcccgcg aagctcttct
     5641 cagcggcgtt cctgtctatt acgccgatga gacgacacca gaaaactgcg ttatcaaaga
     5701 atatccggat ggccgcaggg aactggtgag cttcatgact ggtatggaga aactcgttgg
     5761 ggctaataca tgatttttgg atgtccggat ttcagaacca ttatgacact tgaggggcag
     5821 gaaaaaataa agccccgcct ggtaaggacg gggctttatt tttaatactc tgacctgcat
     5881 ggaacatcct acattagact ggaacattat atatctaatc gcgcccctgg ctgctatttc
     5941 tggtgcgtca gccatgcgtc atgcaaagcc tgcaggttga gccagaaatc tttatcggtc
     6001 tggaactgtt cggccagctt gtaagactcc tcagcactga ggcgacggcg gttactgata
     6061 atatcatcga tatgatgctc aggaattcct gtagcctgcg ccagttcacc ctgagacatg
     6121 ttcagaggtt ttaaaaattc ctctgcgagc atttcccccg cagttgtcgg ctccgcacgc
     6181 ttcccggtat ggaaagccag aatttcagct cgtccctgat cataggttat ctcacccgaa
     6241 ataattctcg ctttaaccga ctcgtagcgg gggcttccac tgggatctat accttccagt
     6301 ctgaggcttg cctcagcctt catgaagcca cgccggcgct gttcttcact catatctacc
     6361 tcatatgtca atatcaggcg tacctcatat gtcatgaaat aatgttaacc agtacaatgc
     6421 attcagcccg aaaagacgcc tgatcagtct ggccgggtca ggtatgcagc atggttggcg
     6481 ctgtgttgca taccgctaac gcccacggcc accacgttca ttctgcttat ccctctcgac
     6541 ttttgcctta aacttctcca gaacctgatc acgcttgctg agtttctctt tctccccgga
     6601 ctccagttct tttgtctctt cgctgagcgt ttcattctgc agtgaagcct gatgagacgc
     6661 tttttccagc tctgcacgtt cggctttgtc ggctttatct ctccgggcat caccacgttg
     6721 tttgagttct tcgatagttt ccagcttcat atcagctttc tcctgtttgc gggactgtac
     6781 gcgggtacgg tatgcagcca tatcttccgc gttcttctga gcattgagcg attttatctg
     6841 cccagccaag atcggatttt ctttcgcggc cagctggtcg gccagccgtt ctcccggcgt
     6901 agacatttca taagcgccag acttacgcat gtagtctctg acaaagccaa gtcgattttt
     6961 cagctgcacc agacgacgat cgaggccatt tttgatggtc tgccaggtac tggcggcctg
     7021 ttcataggcg ctgcgtttaa acattcctat caggccgctg ggctgttctg gtctttgtcg
     7081 ctcatgtgag gatatttttt gtctctggcg ttcaacatgt gccgccccac gggcggcaat
     7141 ccgtccggcc ttatcctgaa cgaaagtcag cttatcccgg cgtaaactct gccattcaag
     7201 ccggataact tccggccgtt ttgacttgct ggttacagtg ttttcccgtc gcagaggctg
     7261 atttctgtcg gttctctggt gctccacgtc acgagataca ttgcccggca ataacatctc
     7321 acttcgttct ccgaagcgaa ccatatcgag ttcggacagg ttgcgcaggc gaccccttgc
     7381 gctgggcggc gggacggctc caacgcggcc agtgtaggac tgagcggtgc ggactcctcg
     7441 cttatctcgc cctttgtctc gttcagggac tggagcaatg cttccatgtc ctgctctgct
     7501 gttgttgtca tgggttgccc tccagttatc aatcagctga attgcccgtg aaagcggctg
     7561 gatttcagca ttacgcaata taatctggcg atattcattg cctcgttcgg tctctaagcc
     7621 gcgcttttcc atctgcgcag ctgcgtaacc gatatgaata gtcggaaccc gatccagctt
     7681 tgctgccttg gctgaatccc cctgagaaat agctgattcc cgttgcgcct caagtgaacg
     7741 atggtcaata cgctctgaac ggccagcctt ttccagatga cggttcgtca tttctgacca
     7801 tgcagaacgc cagtggttga cttcgccgct cttaaaatca tcaagttcac gggtcttatc
     7861 cttgaaacct tcctccgaca gtcggcgggt gctgcataaa acgtgagcat gatgattacg
     7921 ttggtctccc tctttacccg gagcatggat agcggcatcc actgccattc cgtgacgctc
     7981 aaccagcgaa gaacaaaaat cacgaagcag ctcgagacgc tgttttccgt caagctctgc
     8041 aggaaatgcg agttcaaatt ctctggcaac ggttgagtta cgacggtttt ctttctgttc
     8101 aactgcattc catagagtct cccgattact cgcccaatct ggtgagtttt gagggagaaa
     8161 aatctctgaa tgctcgacgc ctgatttacg ggtgtagtcg tggataatcc cagtccgttc
     8221 atcaacaatt cttgaacctg tacgataagc aattgccgca gtggccgaac ggccagcact
     8281 acggctgatt gtcttaacgc taagatgaaa aattgccaat ttcgtattcc tgtttttccg
     8341 ttgaccttgg gtgcagggtt ttaaccctgc cgcacgcaaa ctcgaagagt ttgcataagt
     8401 gcgccctttg ctttcgcttc gggttgaaat atatcagtac aggggttatc gttcaaattt
     8461 gaatcaacga ggtgactaat ggcaaaatca agagcggaaa aattggaaga gttgcgtcag
     8521 aaggaagctc agataaaggc gcaaatccag caattaaagc aaagagagca gacagaagaa
     8581 cgcaagaagg atacccgccg aaaaatccta atcggtggag ccgtactagc aaaaataaag
     8641 cgtggggaat ggaattatca gcagttggtc agtcttctgg acaatgagct gaaaaatgac
     8701 agagaccggg cactgtttga tgaaatgggt ttaaaggcag ttgacgccag aaaagaatct
     8761 tttaaccccg gtaataagtg acgccaatta tttattaccg gatgataaat cagatcagga
     8821 gatttagc
//
