LOCUS       NZ_CP103738             1458 bp    DNA     circular CON 21-SEP-2023
DEFINITION  Klebsiella pneumoniae strain 913 plasmid pMB2930_7, complete
            sequence.
ACCESSION   NZ_CP103738
VERSION     NZ_CP103738.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28173989
            Assembly: GCF_024918875.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 347x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/21/2023 03:19:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,533
            CDSs (total)                      :: 5,409
            Genes (coding)                    :: 5,259
            CDSs (with protein)               :: 5,259
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 64 of 150
            Pseudo Genes (incomplete)         :: 92 of 150
            Pseudo Genes (internal stop)      :: 27 of 150
            Pseudo Genes (multiple problems)  :: 27 of 150
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103738.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="913"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pMB2930_7"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2016-11-25"
                     /collected_by="MDACC"
                     /genotype="ST15"
     gene            complement(670..1110)
                     /locus_tag="M5T23_RS27625"
                     /old_locus_tag="M5T23_27625"
     CDS             complement(670..1110)
                     /locus_tag="M5T23_RS27625"
                     /old_locus_tag="M5T23_27625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000031238.1"
                     /translation="MSKDVERITQINDQGEIIGGFVAVIRPKQKSAFQRHFTMNQDALR
                     ILAKELTGEQFKVLMLMLADLDYENFIQIAQADIAETLGMQKTNVSRAVRALLDVGVIF
                     EGPKVGRSKTYRLNEQFGWKGTVTNHKKALKNGFSVIQGGRI"
                     /name="hypothetical protein"
     Typing          177..437
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.13"
                     /coverage="100.0"
                     /evalue="1.84e-104"
ORIGIN
        1 atgtgcaaat gtaataccgt cacccgcgac gatattccgt ttgccatctg gtcagagctt
       61 cgccccgaca ccccgtatca gtgcgtacgt gctcaatgtg gtttcctgtt ctcttccgct
      121 tcctcgccca ctcgctcact aacgttcggt cgctggggct gcggcgagcg gtgccacctc
      181 tcgcatgctg tcggtgattc cgctgttgtt ctttcgggga tgagtcgtca gatagccctt
      241 caaacgcgct cagaagctct cagacgcacg aggcggggtt taaatgcccg tcagggcatg
      301 gaaggcgcga aaaacgctta cagagcgttt caggaggtca ggcgcgccga tacccctagc
      361 caggggcttt caggtcgcca ggaaccgcgc cagccccctg caaagcccat ttgcacggta
      421 caagcagcca ctcggttggt aggtggtcgt agaccgtcgg tgttacagcg atggggggtc
      481 tggggtctcc ccagacggtt tccggttgcg ctcaaggcgc tgccggaacc gtggcggctc
      541 ggtcgttctg gagatcgggg agcaaaaaca cgcaagggat tttgaggcgg caaaagagca
      601 atgcaggata gacaatccac tagaaattgc atgcaatgtt ctgtggattg tccgcctgcc
      661 gctggctctt taaatccggc cgccctgaat gacactaaag ccgtttttga gtgccttttt
      721 gtggttggtt acggtgcctt tccaaccaaa ttgttcattc agccggtatg tttttgatct
      781 gccgacttta ggtccctcaa agatcacccc aacatccagt agcgcgcgaa cggcgcgact
      841 aacattcgtt ttctgcatcc ctaaagtttc agcaatatct gcttgggcga tctgaatgaa
      901 gttttcgtaa tccagatctg caagcatcag cattaagacc ttgaattgct ccccggttaa
      961 ttccttagcc aagatccgta atgcgtcttg attcatggtg aaatgcctct gaaatgctga
     1021 tttttgttta gggcggataa cggcaacgaa gccgccgata atctcaccct ggtcatttat
     1081 ctgagtgatt ctctcgacat ccttactcat ctttgaaccc tatttgagtt atcaataatg
     1141 ataacagtgt tatcattatt gataactatg tcaactgtaa cccttagatt ttattaatgt
     1201 ttttattctc ctttctgtat ctttatatta ggcgtctaac gcattgattc cattagatat
     1261 ttatcctagc tgctctttgg ggtgtcgggg cgaagccctg acccagtcac gtagcgatag
     1321 cggagtgtat actggcttac tcctctgcaa gcctcgtcag ccatgatttt tgtccgattt
     1381 ctcgctctcc acgttcaaca gctccatcat gtttttgccg tccgttcacc gttaaaacgc
     1441 ctcagaaatc aggacagg
//
