LOCUS       NZ_CP103736             4017 bp    DNA     circular CON 21-SEP-2023
DEFINITION  Klebsiella pneumoniae strain 913 plasmid pMB2930_5, complete
            sequence.
ACCESSION   NZ_CP103736
VERSION     NZ_CP103736.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28173989
            Assembly: GCF_024918875.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 347x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/21/2023 03:19:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,533
            CDSs (total)                      :: 5,409
            Genes (coding)                    :: 5,259
            CDSs (with protein)               :: 5,259
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 64 of 150
            Pseudo Genes (incomplete)         :: 92 of 150
            Pseudo Genes (internal stop)      :: 27 of 150
            Pseudo Genes (multiple problems)  :: 27 of 150
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103736.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="913"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pMB2930_5"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2016-11-25"
                     /collected_by="MDACC"
                     /genotype="ST15"
     gene            join(3898..4017,1..96)
                     /locus_tag="M5T23_RS27590"
                     /old_locus_tag="M5T23_27590"
     CDS             join(3898..4017,1..96)
                     /locus_tag="M5T23_RS27590"
                     /old_locus_tag="M5T23_27590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_072048928.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            225..752
                     /locus_tag="M5T23_RS27595"
                     /old_locus_tag="M5T23_27595"
     CDS             225..752
                     /locus_tag="M5T23_RS27595"
                     /old_locus_tag="M5T23_27595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004883515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016246513.1"
                     /translation="MKCFWACSVSVVLAMSFTVAAAENNDNVTYTKPSAGRDLYLTCKG
                     YQVKGASVINVEKPSYLEGTGEQVEFAIITPVGASDGLKVFFDSWGFSSFGLIESHVMH
                     EHWSLVHVGGRASVKGEKIDYSFVDNQPQEGGSYKPKTGVFLYGCMNSDKRPSWVEKIY
                     TRTGVFRGGIED"
                     /name="hypothetical protein"
     gene            1007..1180
                     /locus_tag="M5T23_RS27600"
                     /old_locus_tag="M5T23_27600"
     CDS             1007..1180
                     /locus_tag="M5T23_RS27600"
                     /old_locus_tag="M5T23_27600"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_179098883.1"
                     /translation="MGDFERTFGAGADFDRIIDENNSEYFREDRRKTKNSKKNSLLFKM
                     LWFGRKLILAKR"
                     /name="hypothetical protein"
     gene            complement(1647..1760)
                     /locus_tag="M5T23_RS27695"
     CDS             complement(1647..1760)
                     /locus_tag="M5T23_RS27695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072035093.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPERHDKTPLAAATGN"
                     /name="replication initiation protein"
     gene            2408..2731
                     /locus_tag="M5T23_RS27605"
                     /old_locus_tag="M5T23_27605"
     CDS             2408..2731
                     /locus_tag="M5T23_RS27605"
                     /old_locus_tag="M5T23_27605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024191642.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQICLDEKPSRAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGTGHDRVQVVAVLMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2940..>3374
                     /locus_tag="M5T23_RS27610"
                     /old_locus_tag="M5T23_27610"
                     /pseudo=""
     CDS             2940..>3374
                     /locus_tag="M5T23_RS27610"
                     /old_locus_tag="M5T23_27610"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3409..3894
                     /locus_tag="M5T23_RS27615"
                     /old_locus_tag="M5T23_27615"
     CDS             3409..3894
                     /locus_tag="M5T23_RS27615"
                     /old_locus_tag="M5T23_27615"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_061390563.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMISQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIQEQKSTQAMLSEKNNGVQLTTCGEERRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     Typing          2118..2295
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.222"
                     /coverage="83.0"
                     /evalue="4.5e-50"
     Typing          complement(1648..2168)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.564"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2722..4016
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.056"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 caacgggaga acgcggcgct gagcgagcgc gtcacgggct tgagcaggca ggtgcagagt
       61 ttgagcgatc agctgcgccg attgtcgcaa ggctgaatgg catcgaggcg caccgggagc
      121 aggagcgcgc ggcgcagcac cagaaagcgc tggagctgga acgatcgcag cggcagaaag
      181 gttttgagct tggaatgtag ttaatcaatg aaaagggatg cgttatgaaa tgtttctggg
      241 cttgctcagt ctcggtggtt ttggctatgt catttacagt cgctgctgcg gagaataacg
      301 acaacgttac atatacaaaa ccgtcagccg ggagggactt atatcttacc tgtaaaggtt
      361 atcaggtgaa gggggcatct gtaattaacg tcgagaaacc ttcttacctt gaaggtactg
      421 gtgagcaggt tgaatttgca atcattactc ctgttggtgc gtctgacgga ctcaaggtct
      481 tttttgattc atggggtttt tcttcatttg gattgattga aagccatgtg atgcatgagc
      541 actggtcttt agttcatgtt ggggggcgtg cttcagtcaa aggtgaaaag attgattatt
      601 catttgtaga taaccagcca caggaggggg ggagctataa acccaaaaca ggtgtgttcc
      661 tgtacggctg catgaactcg gataaaagac catcttgggt cgaaaagatc tacacaagaa
      721 caggtgtgtt tcgagggggc atagaagact aaaatactgc ttacagcatg aaaatccatt
      781 gaacagagtg tcagttcatt tccgtgcata actatgcata ccgtaagaac ttacgggggg
      841 catttatcgg cgtttttacg tggtaagagg tgctcttttg cggttcatgg cgcagaacgg
      901 tgctgaggct gtctgcggcg gtgcgaacaa gggatgttat ggtaaataag agtgtctttt
      961 ggatatttta aatctgtttt tttattaaat ttaatggggg cagaaaatgg gggattttga
     1021 gcgtaccttt ggagctggtg ctgattttga tcggataatt gatgagaata acagcgaata
     1081 ttttcgagaa gatcgccgaa aaactaaaaa ttcaaaaaaa aattctcttc ttttcaagat
     1141 gctttggttt gggcgaaagc taatcctggc aaaacgataa taagatctcc agacggtgtg
     1201 gggtttattg aaaaatgaaa gagtggattc actgtaaaac aacagtcatt ttttggacaa
     1261 cactgtctat atacgtactc atatgtttca cagtgtcgta tgttggagtc tatgttacgt
     1321 acgttgcagg gcctattttg ttagttacag gcattcttgc atggcttacg aaacctagct
     1381 gtaatccaaa gtgactgggc tagacgaact ttatcccccc ataaaacccg cttttttaaa
     1441 cggatcttct tgagatcatt catggagaat cgtaatctct tagcactgta aacgaaaaaa
     1501 ccgcccagca aggcggtttt ttcgaaggtt ctcagagcag caactctttg aaccgaggta
     1561 actggcttgg aggagcacag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc
     1621 ataacttcaa gactaactcc tctaaatcag ttaccagtgg ctgctgccag tggcgttttg
     1681 tcgtgtcttt ccgggttgga ctcaagtcga tagttaccgg ataaggcgca gcggtcggac
     1741 tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt
     1801 gtcaggcgtg gaattagata aacgcggcca taacagcgga atgacaccgg taaaccgaaa
     1861 ggcaggaaca ggagagcgca cgagggagct accaggggga aacgcctggg atctttatag
     1921 tcctgtcggg tttcgccacc actgatttga gcgtcagatt tcgtgatgct tgtcaggggg
     1981 gcggagccta tggaaaaacg gcaacgccgc atcctttttc cgccctttct gattgatttc
     2041 cgcttagtct ctttatccag ccattcgtaa gctggcaacg cccgccgcag tctcacggca
     2101 ggagcgcagc gaccgagtga gcgaggaggc gtcatttcac cgggatctgc atttcgcact
     2161 tacgccccac agcagcattt tctccctgcc acatgaagca ctttcctgaa tccccactcc
     2221 gatttaccat gttaagccag tatacactcc gctagcgcta cgtgactggt tcagggctgc
     2281 gccccgaaac ccgctgaaaa gtactgccgc gccctgcggg cttgcccaac accgctccgg
     2341 acaggaaaaa atttcccgcc cgaaccggtg ttatccggag gatggtttgg cagaaaagcg
     2401 caacaaaatg ctcactatgt gggtgacaga agacgagcac cgccgacttc tggagcgctg
     2461 tgagggtaaa caactcgcgg cctggatgcg ccagatatgc ctggacgaaa agccctcgcg
     2521 tgctggcaaa cttccatcca tctcgccggc gctgcttcgt cagcttgccg gcatgggtaa
     2581 caacctcaac cagattgccc ggcaggttaa cgccggtggc ggtaccgggc acgatcgcgt
     2641 gcaggttgtc gccgtgctga tggccattga tgccggactc gagcggctgc ggcacgccgt
     2701 gctggaaaag ggggcagacg atgatcgtta agtttcatcc cagaggccgc ggcggtggcg
     2761 gcggtccggt ggattatctg ctggggaaag accggcagcg cgacggcgcc agcgttctgc
     2821 agggtaagcc ggaggaagtg cgggagctta ttgacgcctc gccttacgcc aagaaataca
     2881 cctccggcgt gctgtctttt gccgaacagg atttgccgcc cggccagcgt gaaaagctga
     2941 tggcgagctt tgagcgggtt ctgatgcccg gactcgataa agaccagtac agcgtactgt
     3001 gggttgagca ccgggacaaa gggcggctgg agctgaactt cctgatcccg aacacggagc
     3061 tgctgaccgg cagacgtctc cagccgtatt acgaccgcgc cgaccgtccg cgcattgatg
     3121 cctggcagac catagtgaac ggcagactgg ggctgcacga cccgaacgcg ccggaaaacc
     3181 ggcgggcgct ggttacaccg tccgcgctgc cggaagcgaa gcaggaagcc gcccaggcga
     3241 ttacgcgcgg cctactttcc cttgcctcat ccggggagct gaaaacccgt caggacgtca
     3301 ctgaggcgct ggaaagtgcg ggttttgagg tggtgcgcac cacgaaaagc agcatcagca
     3361 ttgccgaccc tgacgggggg cgaaacatcc gactgaaggg agccatctat gaacagtctt
     3421 ttaacgctgg cgaaggactt agagcagaaa tcgaaagcgc agcagcagac taccggcgag
     3481 atgctgaaag ccgcattcag cgagcacgag aagtctgtca gagcggaact gagcgaaagc
     3541 gagaagagaa tcagcgccgc catcctcgac cacgaccgga agctgtcctc agccatgagc
     3601 caacgcacga aagggatgct gcgtatgatc agccagacgt ggctgaccat agtcctggtc
     3661 tccgcgctac tgatcgcctc gagcgcgggc attctgtggt ggcaggggca acagatactc
     3721 gacaactaca cgaccatcca ggaacagaag agcacgcagg ccatgctttc agagaagaac
     3781 aacggcgtcc agctcacgac ctgcggggag gagagacgcc gctgcgtgag ggtgaacccg
     3841 gaagcgggaa gattcggaga ggattcgagc tggatgatac tggcggggaa atagcacatg
     3901 acggaactgg aaaaacagtt gctgagcgca ttagagcagc tacagcagga ctactcgaaa
     3961 aggctggacg agtgggagaa cgccttcgcg gaatggcgga cgatgtctgg gcttatg
//
