LOCUS       NZ_CP103735             4258 bp    DNA     circular CON 21-SEP-2023
DEFINITION  Klebsiella pneumoniae strain 913 plasmid pMB2930_4, complete
            sequence.
ACCESSION   NZ_CP103735
VERSION     NZ_CP103735.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28173989
            Assembly: GCF_024918875.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 347x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/21/2023 03:19:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,533
            CDSs (total)                      :: 5,409
            Genes (coding)                    :: 5,259
            CDSs (with protein)               :: 5,259
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 64 of 150
            Pseudo Genes (incomplete)         :: 92 of 150
            Pseudo Genes (internal stop)      :: 27 of 150
            Pseudo Genes (multiple problems)  :: 27 of 150
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103735.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="913"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pMB2930_4"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2016-11-25"
                     /collected_by="MDACC"
                     /genotype="ST15"
     gene            join(3651..4258,1..25)
                     /locus_tag="M5T23_RS27570"
                     /old_locus_tag="M5T23_27570"
     CDS             join(3651..4258,1..25)
                     /locus_tag="M5T23_RS27570"
                     /old_locus_tag="M5T23_27570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011977833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_029364653.1"
                     /translation="MLERFIDVLNKDVLFDLSATPSPDSSVLVVREDGELSEIKELTIK
                     EIPDDSIAFTLDFSSKKNSKENGALFSQLSPYFDKSNGDGVNKSCDLVLITRDGDKFTV
                     LIFDQKSKKPDIESSFLQLENSRVFVSYVINIISLIYKDNIDEGKIVFKRVIGTTRVVK
                     TGVNAANHEQERSRREKLRALGMNEVSIKRTASIEKGWLDYRGIVSF"
                     /name="hypothetical protein"
     gene            771..962
                     /locus_tag="M5T23_RS27575"
                     /old_locus_tag="M5T23_27575"
     CDS             771..962
                     /locus_tag="M5T23_RS27575"
                     /old_locus_tag="M5T23_27575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000166328.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            962..1363
                     /locus_tag="M5T23_RS27580"
                     /old_locus_tag="M5T23_27580"
     CDS             962..1363
                     /locus_tag="M5T23_RS27580"
                     /old_locus_tag="M5T23_27580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_009310048.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            2225..3649
                     /locus_tag="M5T23_RS27585"
                     /old_locus_tag="M5T23_27585"
     CDS             2225..3649
                     /locus_tag="M5T23_RS27585"
                     /old_locus_tag="M5T23_27585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011977832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_029364654.1"
                     /translation="MEIEFENIGFIKSGKIKTNNITVIFGPNNVGKTYLSYSTYAAISR
                     YNDIFREKIKIPKSVSSNLVLNGTARYDITNVFTDSLVKSVNEEVSSSLPAFFKDSSGM
                     FSKSKISVIDSYIKDRVVNSEVNVNISVSENIILNIQKKKNNTCIDFTVTSSKKNNESG
                     SPVIDLKEKDASNYLNILFNFFFLNEVLDSAANNAFIITSERTGISLFQKEIDGNRSNI
                     VDSYMWSKLSTRKLLSFDDIMSQKVAKFSEPINHNINIIRDFADLKKMKHIMTANGVRR
                     ASKERTDVIKTLESLTGGRYSTSDGEAIFTVSCSDGDKVSMPLSLSSASNKALFLLDIY
                     VRYYMSNNSYLIIDEPELNLHPHNQIKMAELLVRLSNCGVKIIITTHSDYIVKEINNRI
                     MASQLKDKSRLTELGYKEADLIFSSEVNGFTIGNDGAISEVEGNRYGVNAFLFDNVIND
                     IENKADLLYTYLEEGL"
                     /name="AAA family ATPase"
     Typing          complement(247..648)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.611"
                     /coverage="99.0"
                     /evalue="3.5900000000000006e-163"
ORIGIN
        1 ctatcgagga atcgtttcct tttagtctac ttccagtagt ctcaagtgct tgtgttgaga
       61 gaggaagaac ggtatctttc aacatataaa gaaaaaccac cgctaccaac ggtggttttc
      121 ttaaggttta tggagctacc aactcattaa acctaggtag ctggcttgga ggagcacagt
      181 caccaaaact tgttctttca gtttagcctt aacaggcgtc taacttcaag actatctcct
      241 ctaaatcagt taccagtggc tgctgccagt ggcgcttttg catgtcttac cgggttggac
      301 tcaagacgat agttaccgga taaggcgcag cggtcggact gaacgggggg ttcgtgcata
      361 cagtccagct tggagcgaac tgccttcccg gaaccgagtg tcaggcgtgg aatgagaaac
      421 cgcggccata acagcggaat gacaccggta aaccgaatgg caggaacagg agagcgcacg
      481 agggagccac ctatcagaaa tgataggtat ctttatagtc ctgtcgggtt tcgccaccac
      541 tgatttgagc gtctttctgt gatgcttgtc aggggggcgg agcctatgga aaaacgacgg
      601 ccgtgcggcc tttccttctt cccgtcatgc ggctgctcgg ttttctcggc gtttctacgc
      661 atgctcctgc cgctcgccgc agccgaacgg caggagcgta gcgactgagt gagcgaggaa
      721 gcggaaaaga gaacatcctt acactgagca ctgacgctct gaggcctctt atgacgaaac
      781 aacagaaaac agcgctgaac atggctaaat tcatccagaa tcagtctctg ctcctgctgg
      841 agaagcttaa cgagcttgat cttgatgctg aggccgacct ttgtgagaaa ctccacgatg
      901 atgcggagca tcttttccgt acactgtcct cgagactgga tgcattacag gatggtaact
      961 gatggccaga gtgtctatta gtgaggctgc ccgactgacg ggtaagagta gaacaacact
     1021 gcacaggtta attaaagcag gtgacctgtc cacctgtagc ggtgaacgca atgcaaaaat
     1081 ggtagatact tcagagcttt tacgtgtctt tggtcccttt gagcaaccga agggtgaaca
     1141 ccattctgga caggtctctg aacagcgtga tacagcctct gctgaccaga gtgaacaggt
     1201 gattcgtcat ctgaaacagg agattgaaca cctgaacacc ttacttttgt ccaaggattc
     1261 gcacattgac agcctgaaac aggccatgct ccttcttgaa aatcaccagg caaaattaac
     1321 tgaatccccg gcgccatggt ggaaattctg gaaaaaatcc tgatcctgcc atgcttgagt
     1381 tttcctctcc ggccatcccg gggagttcct gaaccttccc aaaatcgcca gatctctgaa
     1441 cgctcctcac cactaaaaat acccacacac tacgcaaaat taaaattttg cggatttgta
     1501 tttaatatca gttagttact ttcctgaaat ttcgtttttt aaattttatt tacatagatc
     1561 cgactctatg acgactcgtc atagagtccc cggcgagtcg tcatggagtc ggtaacgact
     1621 caggactata aaaacaatga gttaataacg acccgactct agacccgact ccccgtttgg
     1681 ttgtttttta gacgtggcga ctctgggtac gactctagtg aagagtcgcc ggtgaatcgt
     1741 gtggtgagtc gtccggcgac ttgccacacg attcaccggc tgtcgatggg cccgattcct
     1801 gaaggtgttg aaaacggttt ttccggctgt gccggcaacg cctcacggcc acgcaataaa
     1861 ttgcgtcccc attcaccgtt ttcagtcggg tttggtgcgg gtttcgagtc gtctggccta
     1921 aattcaggct gaaatctgcc accagtcagt ctggtgcata acgattcatg cccgtaaaac
     1981 gccctggagc gattttgagg cgttttcagg gggtaagacg taggatttaa tgggttacgc
     2041 cccagaatcg ttctgaggcc gttttagcgg tgcgtgtaat gacgcgttat ggtaaatcca
     2101 gtagtttttt gctcattcag gagtggtttt taaatcatca tgtatgaata tcttgtaatg
     2161 gtaggtatag ttagcatgga tattgctaag gataaaagca taacatactt agtgggtgat
     2221 gcgaatggaa atagagtttg aaaatatcgg ctttataaaa agcgggaaaa taaaaaccaa
     2281 taacattact gttatttttg ggccaaataa tgtgggtaaa acatacctaa gttatagcac
     2341 atacgctgct attagtaggt acaatgacat atttagagag aaaataaaaa taccaaaatc
     2401 agtatctagc aacctggttt taaacggaac tgccaggtac gacatcacaa acgtttttac
     2461 tgattctctt gttaaatcag ttaatgaaga ggtttcatca agcttacctg cattcttcaa
     2521 agatagttca ggtatgtttt ctaaatcaaa aattagtgtg attgactctt atattaaaga
     2581 tagagttgtt aattctgaag ttaatgtaaa tatttctgtc agtgaaaata taatattaaa
     2641 tatacaaaag aaaaaaaaca atacatgtat tgattttaca gttacctcat ccaaaaagaa
     2701 taatgagtca gggagtcctg tcattgatct gaaggaaaaa gacgcttcaa attatttaaa
     2761 tattttattt aatttctttt tccttaatga ggttttagac tctgcagcta ataacgcatt
     2821 tataataaca tctgagcgta ctggaatatc cttgtttcaa aaggagattg atggtaatcg
     2881 tagtaatatt gttgattctt acatgtggag taagttgtct acgaggaagt tactgtcttt
     2941 tgatgacatt atgagccaga aagttgctaa gttctcggag ccaattaatc acaatataaa
     3001 tataataaga gactttgctg atctgaaaaa aatgaagcat ataatgacgg caaatggtgt
     3061 tagaagggca tctaaggagc gaactgatgt tataaaaaca ctagagtcac ttactggtgg
     3121 tagatactca acatctgacg gtgaggctat ttttacagtt tcttgcagtg atggagataa
     3181 agtgtcaatg ccactttctc tctcatcagc atctaacaaa gcactatttc tattagatat
     3241 atatgtaaga tactacatgt ctaataactc ttacttgata attgatgagc cagagctgaa
     3301 tctgcatcca cataatcaaa ttaaaatggc agagttgctt gtaagacttt caaattgcgg
     3361 agttaaaatt ataatcacaa cgcatagtga ttatatcgtt aaagagatta ataatagaat
     3421 aatggcttcg cagctgaaag ataaatcacg attaactgaa cttggataca aagaagcaga
     3481 tttgattttt tcgtcagagg ttaatggttt tactatcggt aatgatggtg ccatctctga
     3541 ggttgaaggg aataggtatg gggtgaatgc ttttcttttt gataatgtta ttaatgatat
     3601 agaaaacaag gctgatctct tgtatacata tttagaagag gggctctaat atgctggaga
     3661 ggtttattga tgttttaaat aaagatgttt tgtttgattt gtcggcaact ccatctcctg
     3721 actcttcggt gttagttgtt cgtgaagatg gtgagcttag tgaaataaaa gagttaacta
     3781 taaaagaaat acctgatgat tcgattgctt tcactttgga tttttcaagc aagaaaaact
     3841 ctaaagaaaa tggcgcttta ttttctcaat tgtcgccata ctttgacaag tctaatggcg
     3901 atggggtgaa taaatcttgt gatcttgtat tgattacaag agatggcgat aagttcactg
     3961 ttcttatttt tgaccaaaag tcaaaaaaac ctgatataga atcatcattt ttgcagttgg
     4021 aaaactcaag agtttttgtt tcctatgtaa ttaatattat ttctttaatt tataaagaca
     4081 atattgatga ggggaagatt gtttttaaac gagttattgg gactacaagg gttgtaaaaa
     4141 ctggagttaa tgctgctaat catgagcagg aacgatcgag aagagagaag cttcgtgctc
     4201 ttggcatgaa tgaagtctcg ataaagcgta cggcatcaat agaaaaggga tggttgga
//
