LOCUS       NZ_CP103734             5463 bp    DNA     circular CON 21-SEP-2023
DEFINITION  Klebsiella pneumoniae strain 913 plasmid pMB2930_3, complete
            sequence.
ACCESSION   NZ_CP103734
VERSION     NZ_CP103734.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28173989
            Assembly: GCF_024918875.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 347x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/21/2023 03:19:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,533
            CDSs (total)                      :: 5,409
            Genes (coding)                    :: 5,259
            CDSs (with protein)               :: 5,259
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 64 of 150
            Pseudo Genes (incomplete)         :: 92 of 150
            Pseudo Genes (internal stop)      :: 27 of 150
            Pseudo Genes (multiple problems)  :: 27 of 150
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103734.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="913"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pMB2930_3"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2016-11-25"
                     /collected_by="MDACC"
                     /genotype="ST15"
     gene            join(5190..5463,1..851)
                     /locus_tag="M5T23_RS27545"
                     /old_locus_tag="M5T23_27545"
     CDS             join(5190..5463,1..851)
                     /locus_tag="M5T23_RS27545"
                     /old_locus_tag="M5T23_27545"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_021529456.1"
                     /translation="MSGGDGRGPGNTGLGHNGGKPSGNVNGSSGHGGPSSGGGYHYNPG
                     KGPASTVGPDGEVHINITGGMEKDPPGKHHGNGGSGNGHGAGNHNSNSGGNGKTTSIPG
                     WSPDSGKPGRYLPTNKDKLIKGLIQSIDDHSGTYTIHYTGGAVATVTVPNGDVNKMNVH
                     YNIGSGPSSGNKLTRDTIKSFLEFKAENEANAVNDAISKVNDFYKELASKYGDKLEKEA
                     KALADEAKGKKIRNANEAIKAFDRYKDVLNKKFSVSDREAIAKALESLNKDQMAKQLKI
                     FGKAFGVVGEAIQWGGFISGLVKGFRTGDWNEAFISGEKIAVGKVASVMVTVAFSAMAV
                     NPIGILGFAVIMAVTSALITDERLKQLNSFINGI"
                     /name="colicin-like pore-forming protein"
     gene            1652..2671
                     /locus_tag="M5T23_RS27550"
                     /old_locus_tag="M5T23_27550"
     CDS             1652..2671
                     /locus_tag="M5T23_RS27550"
                     /old_locus_tag="M5T23_27550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271364.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREDAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSDKGKKS
                     AAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKKQIIKC"
                     /name="replication initiation protein"
     gene            complement(2932..3330)
                     /locus_tag="M5T23_RS27555"
                     /old_locus_tag="M5T23_27555"
     CDS             complement(2932..3330)
                     /locus_tag="M5T23_RS27555"
                     /old_locus_tag="M5T23_27555"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222916.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            3507..4433
                     /locus_tag="M5T23_RS27560"
                     /old_locus_tag="M5T23_27560"
     CDS             3507..4433
                     /locus_tag="M5T23_RS27560"
                     /old_locus_tag="M5T23_27560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001736644.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIEGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     PTQQKEQLRELRKRWEVKHNEHMRKNGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIMEWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQDRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            4436..4969
                     /locus_tag="M5T23_RS27565"
                     /old_locus_tag="M5T23_27565"
     CDS             4436..4969
                     /locus_tag="M5T23_RS27565"
                     /old_locus_tag="M5T23_27565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021539385.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQNLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVILED
                     KLPEKPVTGKNRKN"
                     /name="hypothetical protein"
     Typing          1653..2427
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.907"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          3508..4430
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 attcaggcgg caatggaaaa acaacgtcta ttcctggatg gagccctgac tctgggaaac
       61 ctggcagata tttaccgaca aataaagata agttaattaa gggacttatt cagtcaatag
      121 atgaccattc tggaacgtac actatccatt atactggtgg tgcagtagct acagtaactg
      181 taccaaacgg tgatgtgaat aaaatgaatg ttcactataa tatcggtagt gggccgtcat
      241 caggtaataa actgactcgt gatactataa aaagcttttt agaattcaag gcagaaaatg
      301 aagctaatgc tgtaaatgat gcaataagca aagttaatga cttttataaa gagttagcat
      361 caaaatacgg cgataagttg gagaaagaag ccaaggcact agccgatgaa gctaaaggga
      421 aaaaaattcg caatgctaat gaggcgatta aagcctttga taggtacaag gatgtgctta
      481 ataagaaatt cagcgtatcc gaccgagaag cgattgcaaa agcactggag tctcttaata
      541 aagatcaaat ggctaaacag ttaaaaattt ttggtaaggc ctttggtgtc gtaggtgagg
      601 ctattcagtg ggggggattt atcagtggct tagttaaagg attcagaact ggtgactgga
      661 atgaggcttt tataagcggg gaaaaaatcg cagtcggcaa ggtagcatct gttatggtta
      721 cagtggcatt tagtgctatg gcagtaaatc ctattggtat tttagggttt gcagtaatta
      781 tggcagtgac tagtgcctta attacagatg aaaggctgaa acaactaaac agctttatta
      841 acggcattta ataaacaaaa ggtcgtaccg tttgatatgg tatgaccttt attttgtttt
      901 tctatcgctt tttgttggtt ataaagtaat agactgtaaa aatcaatcca aatggaggta
      961 gtgataatat aatgcatatt ataaaacccc agttaacgaa gttaaaagtt gtttttgtta
     1021 gtaagggtgc ttgcttattt attagtaata gacaaaaagg gtatgtggac gatccaaaaa
     1081 gagatataag tataaatata aataattcta tacttgttgg gcttagattc aaacttgcga
     1141 tgataaaaga taaaatgtaa aaggataagg caaggataaa attccttaaa tagaattcca
     1201 tatctatttt tttcatttat aaaatcctcg ttagttaaat gtttataata ttgccttact
     1261 ttatccataa atctagaagt agttatcaca aaagttactt ttttgactcg ttcgcccttt
     1321 tgaacggtcg aaactgtttg cgaatcgctt tcgtgcatag ttatgcaacg ccctgaaagt
     1381 gattctgacg catttttctg gtttggcctg gtgtttcctt gtctttttgc gttttttgcg
     1441 ccagaaagcg actgagggcg ttttaagggg tacgtacaac gggagttatg gtaaatggat
     1501 cggttttgcg ggaaggctcg acaggttttg ttgttggggg tagtgtaagc gactgaaaaa
     1561 caaacgcccc tgaaattcgt gtctctccgc caagattgtc acgagaatac aggggcgagg
     1621 tcggtcgata acctggaagg aatcgtaaca catgagcgcc gcgcttcaat acttcgaaga
     1681 aaatttaccc caccgcccct atcacacgga tgatctcgct ttcggccttc gcatctccgg
     1741 caaagggcgt gcgcttcttg cgcggtacat ccagcagaac cagcctcatg cgcagttctg
     1801 gctggttttt gacgttgacc gcgagggggc tgcgattgac tggagcgacc ggaacgcccc
     1861 cgcgccgaac atcaccgtta aaaatcctgt gaacggacac gctcacctgc tctacgcact
     1921 caacatcgcc gtgagaaccg cgcctgatgc ctcggttaag gcgctgaaat atgccgcagc
     1981 ggttgaacgt tcgctgtgtg agaaactttg tgctgatgtg aactacagcg gcctgatttg
     2041 caaaaatccg ttccacctgg aatggcaggt gatggagtgg cgcgaggacg cctataccct
     2101 cgatgaactg gcagattatc tcgatttgag cgcctcagag cgtcgtagca tcgataaaca
     2161 ttacgggatg gggcgaaact gccacctgtt cgaaatgacg cgcaaatggg cttacagggc
     2221 gattcgtcag ggctggccag cgttctcaca gtggcttgaa gcggtgatcc agcgtgtcga
     2281 aatgtacaat gcatcgcttc ccgttccgct ttcactggct gaatgtcggg ctattggcaa
     2341 gagcattgcg aaatacacgc acaggaactt cacgccggaa actttcgcac agtatgtggc
     2401 tgatacgcac acgccagaaa ttcaggcagc acgcggtcgc aagggcggga aggccaattc
     2461 gtcagaaaat cagtcggaca aagggaaaaa aagcgccgct gttcggtgga ctgctaacga
     2521 cgacaaacgt cgtagggcgc ttgatatgta tatcttggga gcgtcaacgg aagatattgc
     2581 agtggcggtt ggtgttagca gccggactat tcgacgctgg atggataatt ctggagaatg
     2641 gctaaccaag aaacaaataa ttaaatgtta gttaacaaag aagcaacact accccccgtt
     2701 ttttgttaat gggatgttaa attcgcggac atgaacaagc ctatatcaga taacagcgcc
     2761 tttcggcgtc tttttgagca gcgggctttc cggcggtttt ctcctctcag cccagcaatg
     2821 gtgcggctta ccgcatgatt gaggtggtcg tgctcgccgc agtctcatga ccgagcgtag
     2881 cgagcgaatg agcgaggaag caccagggaa ccggaacacg gatggaggca cttacgcgtc
     2941 tgggtttggg gatgtctcca gatcgtactt aatttcctta agtgcctgta atatcctgtc
     3001 ttttttatag catagctcgc ctgtttttgt gtctaccagt cctgcttcga tcagtagccc
     3061 agccgcgaga aagagtcggt gattgcgttc tcgtcgttgt ttttttccct cgattcgttt
     3121 ttggaccgcc tgttttcgtt cgctctcctg tgcctgtaag tcgttttttt ccgctgttgc
     3181 cagtacgtgg aggagtttgc gttcctgttc tgtgagtcct gcttcgttgt cgagcaggtc
     3241 aataatctgt ctctgtcgtg cggtctgttc tccgagcttc ctgatgaatc cgatccgttt
     3301 cttcagccat tcgatgttgc tgatgcgcat gttcgtggtg ctccctggtg tttttttgcg
     3361 gtgtgttgtg gtcgtgcagt atatcgcttg cggtcggcgg gtgcaggggg tatcttccgt
     3421 gctaattcgt aaaattagaa ggaagcgcac ttatatgttt ctctccttcg tcgagaaaca
     3481 gtgcgctcct gcggagggct gaaaccgtgg cgacctatca cctcagcgtt aagtttggtg
     3541 gcaagggaca ggctgcaaac catgccgatt acatcgagcg caaagagaaa taccgcgacc
     3601 gtcaagattt ggaatattcc gcacacggaa acatgccgga atgggcgcga gataatccct
     3661 cccatttctg gcaggcggct gaccagtttg aacgtgcaaa cggttcgact tatcgggaac
     3721 tggagatagc gctgccccgt gaactgacac cggaacagcg ccttgaactg gtgcaggatt
     3781 ttgtacgtca ggaagctggc gaacggcatg catggagttt tgccattcac aatccgaaag
     3841 cgagcattga gggaggcgaa cagcctcatg cgcatatcat gatgagtcag cgtgtgaatg
     3901 atggcattga acgtacaccg gaacagtatt tccggcgcta taacgccagg tatcccgagc
     3961 ggggaggtgc gaaaaaggat tcaggaagcc tgactcccac tcagcaaaaa gagcagttgc
     4021 gggaattgcg aaaacgctgg gaggtgaagc acaacgaaca tatgagaaaa aatggctttg
     4081 aaaggggctt cattgactgt cggacgctga aagaacaggg cattgaacga cgtcccgaag
     4141 tgcatctggg ttttgaagct gccggacggc ttgatgatgc ccagcgtcat gacatcatgc
     4201 aaaaacggtc tgaaccggac tattcccgcc atcttgaaca ggcgcgaacg gtcgggcatg
     4261 acatcatgga atggctggac actccggtta agcaacatga actggtcaga aggctgcaac
     4321 atgatgttgc gcgggagcat ggctttgaca tgagagacag agtgagcaga caggaccgaa
     4381 ctgaacctga acgggttaaa tcaccgtcac gtggattttc tatgggattc tgaacatggc
     4441 cagcacagaa gaaaaattac tcacgctcct gttatcggtg gagcatttgc aacataccgc
     4501 aatggagcaa cagaaagctg ttgctgattc tctggctgtg gcaagggaaa cacttcgcgg
     4561 atatcgggat atttctgacg gtgtggcaaa acaggtcagg gatgaagtca gaaatgaggt
     4621 gcagaatctg gacgtagcag ggctgatagg tgagcgcatc agccaggatt ttcagcggct
     4681 ggaagagtca gtcgcaaaca tgaaggcaaa cgcggatgcc ctgaacaggg atattgcgac
     4741 aattcacagc aatatcgtgc aggaatacaa cacccttaaa gggtatagct ggaaatggct
     4801 gggtggggtg tcagtggctt ttctagttat gttctgtgtg ctcggatggg gaatgaaaac
     4861 aatgctcgac aggaactaca acgccacagc ggcggtgtat cagaaagttg tgatactgga
     4921 agataaactg ccggaaaaac ctgtcacggg taagaaccgg aaaaactgac gcgcttcgct
     4981 tgtccaacgc ctcacggaca gcgaaaggat ttcgcgttcc gttcaccgtt ttcatgagtg
     5041 attcctccga actgccaaaa aatatccccc cctgacttga cagaaaaaat aatggcgagt
     5101 actttttgat ctgtacataa aaccagtggt tttatgtaca gtattaacta tgtaattaat
     5161 tgttttaacg attaaaagag ggaattttta tgagtggtgg agatggtcgt ggccctggta
     5221 atacagggct tggacataac ggtggtaaac ccagtggaaa cgtgaatggc tcatctggac
     5281 atggaggacc atcatctggc ggtgggtatc attacaatcc gggaaaaggt cctgcaagca
     5341 ctgtaggtcc tgacggggag gtgcatatca acattaccgg tggtatggaa aaagatcctc
     5401 caggaaaaca tcacggtaat ggtggctcag gtaatggtca cggtgctgga aatcataaca
     5461 gca
//
