LOCUS       NZ_CP103733             9656 bp    DNA     circular CON 21-SEP-2023
DEFINITION  Klebsiella pneumoniae strain 913 plasmid pMB2930_2, complete
            sequence.
ACCESSION   NZ_CP103733
VERSION     NZ_CP103733.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28173989
            Assembly: GCF_024918875.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9656)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 9656)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 347x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/21/2023 03:19:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,533
            CDSs (total)                      :: 5,409
            Genes (coding)                    :: 5,259
            CDSs (with protein)               :: 5,259
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 64 of 150
            Pseudo Genes (incomplete)         :: 92 of 150
            Pseudo Genes (internal stop)      :: 27 of 150
            Pseudo Genes (multiple problems)  :: 27 of 150
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103733.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9656
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="913"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pMB2930_2"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2016-11-25"
                     /collected_by="MDACC"
                     /genotype="ST15"
     gene            complement(174..536)
                     /locus_tag="M5T23_RS27495"
                     /old_locus_tag="M5T23_27495"
     CDS             complement(174..536)
                     /locus_tag="M5T23_RS27495"
                     /old_locus_tag="M5T23_27495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019105480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045889489.1"
                     /translation="MAWHSVSQAQNMTGKSRRTLYRDMASGRLSWRTNTSGHRELETSE
                     LIRVYGEIKNDGTAERHTVSQPDVTVSGSDIRDELRALRQEVADLKSMLLRLEHKQEEQ
                     PETAAPWWRFWQKKST"
                     /name="hypothetical protein"
     gene            896..1036
                     /locus_tag="M5T23_RS27500"
                     /old_locus_tag="M5T23_27500"
     CDS             896..1036
                     /locus_tag="M5T23_RS27500"
                     /old_locus_tag="M5T23_27500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_062939423.1"
                     /translation="MQEPPVQPDCCALPGNYRLESNPGRHDKAPLAAAIGNWEFAENLF
                     S"
                     /name="hypothetical protein"
     gene            complement(1424..1918)
                     /locus_tag="M5T23_RS27505"
                     /old_locus_tag="M5T23_27505"
     CDS             complement(1424..1918)
                     /locus_tag="M5T23_RS27505"
                     /old_locus_tag="M5T23_27505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045889487.1"
                     /translation="MAGTVWNDVNTGREVFSMTHLHPQLHKVEVSGVQAEIEISFGFHV
                     FTDQKQVGKVMSFRGETRHFCQERYEGSKTIVQRILSAIENGEYITAFISKGQGQRYYH
                     LNHHDDFILMEIRKPEGKDNFLRIHVVTAYTLDEWGTVNKGRNLRFRYVLEQRLQGKKI
                     V"
                     /name="hypothetical protein"
     gene            complement(1922..2440)
                     /locus_tag="M5T23_RS27510"
                     /old_locus_tag="M5T23_27510"
     CDS             complement(1922..2440)
                     /locus_tag="M5T23_RS27510"
                     /old_locus_tag="M5T23_27510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045889486.1"
                     /translation="MSVRAQRAGKRNQSERRIELINTLWEGEEIETWDRNDRPRNDGFI
                     TVPRYLPLLGVLMDELSKGSPLSSTYLALWFRVSDEGLIEIRDKTVLALESGFASGRGV
                     TTWTGRMRKLKELGFISCREGSSGEFHNVLIVHPLVAVKKLLDEGKITKGKTYNTFAER
                     VIEVKSSWE"
                     /name="hypothetical protein"
     gene            2857..3210
                     /locus_tag="M5T23_RS27690"
     CDS             2857..3210
                     /locus_tag="M5T23_RS27690"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_230797240.1"
                     /translation="MITQLKGRKIALVSFCWYELICTQMNGNQFSQEKKVKGSKPSIWS
                     ITEGEVIMATINLSLLEHDCGEAAREALLSGVPVYYADETTPENCVIKEYPDGHRELVS
                     FMTGMEKLVGANT"
                     /name="hypothetical protein"
     gene            complement(3371..3682)
                     /locus_tag="M5T23_RS27520"
                     /old_locus_tag="M5T23_27520"
     CDS             complement(3371..3682)
                     /locus_tag="M5T23_RS27520"
                     /old_locus_tag="M5T23_27520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_259431153.1"
                     /translation="MQQCQITYDQGRAEILAFHTGKRAEPTTAGEMLAEEFLKPLNMSQ
                     GELAQATGIPEHHIDDIISNRRRLSAEESYKLAEQFQTDKDFWLNLQALHDAWLTHQK"
                     /name="HigA family addiction module antitoxin"
     gene            complement(3722..4426)
                     /locus_tag="M5T23_RS27525"
                     /old_locus_tag="M5T23_27525"
     CDS             complement(3722..4426)
                     /locus_tag="M5T23_RS27525"
                     /old_locus_tag="M5T23_27525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            complement(4761..6584)
                     /gene="mobQ"
                     /locus_tag="M5T23_RS27530"
                     /old_locus_tag="M5T23_27530"
     CDS             complement(4761..6584)
                     /gene="mobQ"
                     /locus_tag="M5T23_RS27530"
                     /old_locus_tag="M5T23_27530"
                     /inference="COORDINATES: protein motif:HMM:NF015354.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_072052381.1"
                     /translation="MAIFHLSVKTISRSAGRSATAAIAYRTGSRIVDERTGIIHDYTRK
                     SGVEHSEIFLPQNSPDWASNRETLWNAVEQKENRRNSTVAREFELAFPAELDGKQRLEL
                     LRDFCSSLVERHGMAVDAAIHAPGKEGDQRNHHAHVLCSTRRLSEEGFKDKTRELDDFK
                     SGEVNHWRSAWSEMTNRHLEKAGRSERIDHRSLEAQRESAISQGDSAKAAKLDRVPTIH
                     IGYAAAQMEKRGLETERGNEYRQIILRNAEIQPLSRAIQLIDNWRATHDNNSRAGHGSI
                     APVPERDKGRDKRGVRTAQSYTGRVGAVPPPSARGRLRNLSELDMVRFGERSEMLLPGN
                     VSRDVEHQRTDRNQPLRRENTVTSKSKRPEVIRLEWQSLRRDKLTFVQDKAGRIAARGA
                     AHVERQRQKISSHERQRPEQPSGLIGMFKRSAYEQAASTWQTIKNGLDRRLVQLKNRLG
                     FVRDYMRKSGAYEMSTPGERLADQLAAKENPILAGQIKSLNAQKNAEDMAAYRTRVQSR
                     KQEKADMKLETIEELKQRGDARRDKADKAERAELEKASHQASLQNETLSEETKELESGE
                     KEKLSKRDQVLEKFKAKVERDKQNERGGRGR"
                     /name="mobQ"
     gene            6744..7046
                     /locus_tag="M5T23_RS27535"
                     /old_locus_tag="M5T23_27535"
     CDS             6744..7046
                     /locus_tag="M5T23_RS27535"
                     /old_locus_tag="M5T23_27535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045889494.1"
                     /translation="MAKSRAEKLEELRQKEAQIKAQIQQLKQREQTEERKKDTRRKILI
                     GGAVLAKIKRGEWNYQQLVSLLDNELKNDRDRALFDEMGLKAVDARKESFNPGNK"
                     /name="hypothetical protein"
     gene            7094..9577
                     /locus_tag="M5T23_RS27540"
                     /old_locus_tag="M5T23_27540"
     CDS             7094..9577
                     /locus_tag="M5T23_RS27540"
                     /old_locus_tag="M5T23_27540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934115.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA transfer
                     family protein"
                     /protein_id="WP_199051308.1"
                     /translation="MKKNILAGIFFVAMLLPVWHAAAETFTRCHPNETVWAMIQDEQLK
                     NIARLQETKRCQAVNDTYDQWRQNFQAMGDAENAIRSARDHQNWNAVKSTFDNIAPQIN
                     SLHALAEKNSSLLGAGNILALFGGDLNYLYQNAGLVAPASADGLTDNVSQMLTEPDKQV
                     KAAGIAQQLVYQTRVRAQSFIQGVAQNAGNEAQKEYRAQSDKIVEDKAHNFKGDSVEGY
                     FGGFLARISQVYAPVLLFLIIVGIIGALTARRVRLDAFTASVKAVMAAAVPAVALALML
                     IFMPFLPSWVLYAAALAVTFAIYSRSKKILGGLASRCGRFPALASGLRWMGGFVSGLDA
                     DRKQETGAASINPASHHTHGSSRWGTAAEMRENNHLLPTEKQAGFTLARELSPPPGVDG
                     KFCFTGHVVTVAPTGSGKGIGAVIPNLLTYQGSALVFDPKGENAEVTGRRRREMGQSVH
                     VIDPFGITSGIKESVNILDRLDVTSPDCVAESAVLAESLVVSEKGGDGNHFDESARTLL
                     QGLMLHVSGLPDESRRNLAELRRLITSDEENLLALLADMAASEDVAFGIPARAANTLMG
                     MADRERGSVLSTARKNTAFLDDPRIAAALSHSDFNLSAIKAEPMTVYLVLPANRIAANT
                     RFVRLFIGSVISAVTESKAKPAHNVAFILDEFNVLGYMKSIEDAVSLLRGYGLSFWVFL
                     QDLSQLKSAYPKWQTFLANSAKTFYGTDDYDTAKYVSDTLGKSTVEFETQNSGRNSGGG
                     VSGGGGSMNRGRSSGSSQQFAGRELLTPDEVMRLGPTRPIVILKGEYPYQLTRINYLTD
                     PEYTGMADPNPYYSA"
                     /name="type IV secretory system conjugative DNA transfer
                     family protein"
     Typing          638..1005
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.566"
                     /coverage="85.0"
                     /evalue="8.49e-135"
ORIGIN
        1 tgtgataggc ataccgatat acttctcttt ccgctttgtc tgtgccttta tctgggctct
       61 ggtgacgcaa aacagaagac acagtgctat gccaatcgag gcacaaaaga ccaccaatcc
      121 tgaaacacta ccggataacg taatcaactt tagccgcgaa aaagagaaaa gggttaagta
      181 gattttttct gccagaaacg ccaccacgga gccgcagttt caggctgctc ttcctgttta
      241 tgttccagcc tcaaaagcat agatttaagg tctgcaactt cctgccgtaa ggcacgcaat
      301 tcatccctga tgtcactacc ggatactgtg acatcaggct gtgacacggt gtgccgctca
      361 gctgtgccat cgtttttaat ctcgccataa acacggatca gttctgaggt ctcaagttcg
      421 cgatggccgc tagtattagt tcgccatgac aagcggccag acgccatatc acgataaagg
      481 gtcctgcgac ttttccccgt catgttttgt gcttgtgaca cactgtgcca tgccataaca
      541 ccaccttgat taaatgacac actctacgag ctgcactcta gcaggctaac ggaatccagg
      601 cacgcggtaa gaaagcaaag agaaaagcgc cagacccgtt tttccatagg ctccgccccc
      661 ctggcgagca tcacaaaaac agacgctcaa gccagaggcg gcgaaacctg acaggactat
      721 aaagatcatc accgtttccg gtgaatcgct ccctcttgcg ctctcctgtt ccgaccgtgt
      781 cgctttaccg gatacctgac cgcctttttc ccttacggga catgtggcgc tttctcatag
      841 ctcacactgt aggtatctca gctcgatgta ggtcgttcgc tccaagctgg gctgtatgca
      901 agaacccccc gttcagcccg actgctgcgc cttgcccggt aactatcgtc ttgagtccaa
      961 cccgggaaga cacgacaaag cgccactggc agcagccatt ggtaactggg agttcgcaga
     1021 gaatttgttt agctaaacac gcggttgctc ttgaagtgtg cgccaaagta cggctacact
     1081 ggaaggacag atttggttgc tgtgctctgc gaaagccagt taccacgatt cagaaacttg
     1141 ccggtttact gaaccttcga aaaaccacct tgccgggtgg ttttttcgtt tacaggacaa
     1201 gagactacga cgataaacaa aggatctcaa gaagatcatg acaaacccgt gaagttgaca
     1261 agtggcattg gtaagtggac aatatacctg tcagcacaaa ccggaacctt gaaagagggg
     1321 tgctgatgcc gggggcaacc ctcctagcca gtgagcggga taacgaaagg ccagaccgaa
     1381 atttttgcag taaaacaaag gcgccttggg cgctttttgt tttttatacg attttcttcc
     1441 cctgtaacct ctgctccaaa acatacctga acctcaaatt ccgtcctttg ttgaccgtcc
     1501 cccattcatc cagcgtatat gccgtaacaa catgtatgcg aagaaaatta tctttacctt
     1561 caggctttct tatctccatt agaatgaagt catcatgatg gttaaggtgg taataccgct
     1621 gcccttgccc cttggaaata aaggccgtta tgtactctcc gttttctatc gctgacagta
     1681 tccgctgcac gatagtctta cttccttcgt atctttcctg gcagaaatgc cgagtttcac
     1741 ctctgaaact cattaccttt ccgacttgct tttggtcagt aaatacatga aacccgaagc
     1801 taatctcaat ctcagcctgc accccagaaa cctcaacctt atgcagctga gggtgcaggt
     1861 gtgtcatcga gaaaacttca cgccctgtat taacatcatt ccataccgta cctgccattt
     1921 cttactccca tgaggacttc acctcgatta cccgttcagc aaaagtgtta taggttttcc
     1981 ctttcgttat tttcccttca tccagtagtt tctttaccgc aaccagcgga tgaacaatca
     2041 acacgttgtg gaactcaccg gagctacctt cccggcatga tatgaatccc agctctttta
     2101 atttccgcat tctccccgtc catgttgtta ccccccttcc actggcaaag cctgattcta
     2161 aagccagcac agtcttatcc cttatctcaa tcaggccttc gtctgaaacc ctgaaccaca
     2221 acgctaggta ggtacttgaa agtgggcttc cctttgataa ctcatccata agaacaccta
     2281 gtaatggaag ataacgaggt actgttatga agccatcgtt tcgaggtcgg tcattacgat
     2341 cccatgtttc aatttcctcc ccttcccata atgtatttat cagttcgatt cttctttcag
     2401 actggttacg tttccctgct ctttgtgccc tgactgacat actcgcctcc tgttaatgca
     2461 tgagagctaa tttaccaaaa aatccttata aatcaataac ttttataaat tccctcggac
     2521 aattacttcc agtttttatt ttttttaacc ataaaaaatg caatatttat tacataaacg
     2581 gcagttataa gtaatatata ttacaaaaag aattcgcatt aacatgattt aaaaagagtt
     2641 tttatttaat tgcggtgctc catgtgctca ccgtgctcca tgtgcttccg gtgctccctt
     2701 gtgctccatg gggttgtagg gggcaaggag gggtggagaa atcgaaaaat aaattaagag
     2761 tcaccatgcc ggtcactcgt atttaccata acatccgtta gcagaaccgc ctcagaggcc
     2821 ctcagcgcgt ttttatgccc taagcctcaa aagaccatga ttacccaatt aaaaggccgt
     2881 aaaatcgcac tggtgagttt ttgttggtat gaattgatat gcacacaaat gaatggcaat
     2941 caatttagcc aggaaaagaa ggtcaaaggg tcaaaaccat ccatttggag tattaccgaa
     3001 ggagaagtca tcatggcgac cattaatcta tcactactgg aacatgactg cggcgaagcc
     3061 gcccgcgaag ctcttctcag cggcgttcct gtctattacg ccgatgagac gacaccagaa
     3121 aactgcgtta tcaaagaata tccggatggc cacagggaac tggtgagctt catgactggt
     3181 atggagaaac tcgttggggc taatacatga tttttggatg tccggatttc agaaccatta
     3241 tgacacttga ggggcaggaa aaaataaagc cccgcctggt aaggacgggg ctttattttt
     3301 aatactctga cctgcatgga acatcctaca ttagactgga acattatata tctaatcgcg
     3361 cccctggctg ctatttctgg tgcgtcagcc atgcgtcatg caaagcctgc aggttgagcc
     3421 agaaatcttt atcggtctgg aactgttcgg ccagcttgta agactcctca gcactgaggc
     3481 gacggcggtt actgataata tcatcgatat gatgctcagg aattcctgta gcctgcgcca
     3541 gttccccctg agacatgttc agaggtttta aaaattcctc tgcgagcatt tcccccgcag
     3601 ttgtcggctc cgcacgcttc ccggtatgga aagccagaat ttcagctcgt ccctgatcat
     3661 aggttatctg gcactgttgc aaagttagcg atgaggcagc cttttgtctt attcaaaggc
     3721 cttacatttc aaaaactctg cttaccaggc gcatttcgcc caggggatca ccataataaa
     3781 atgctgaggc ctggcctttg cgtagtgcac gcatcacctc aatacctttg atggtggcgt
     3841 aagccgtctt catggattta aatcccagcg tggcgccgat tatccgtttc agtttgccat
     3901 gatcgcattc aatcacgttg ttccggtact taatctgtcg gtgttcaacg tcagacgggc
     3961 accggccttc gcgtttgagc agagcaagcg cgcgaccata ggcgggcgct ttatccgtgt
     4021 tgatgaatcg tgggatctgc cacttcttca cgttgttgag gattttaccc agaaaccggt
     4081 atgcagcttt gctgttacga cgggaggaga gataaaaatc gacagtgcgg ccccggctgt
     4141 cgacggcccg gtacagatac gcccagcggc cattgacctt cacgtaggtt tcatccatgt
     4201 gccacgggca aagatcggaa gggttacgcc agtaccagcg cagccgtttt tccatttcag
     4261 gcgcataacg ctgaacccag cggtaaatcg tggagtgatc gacattcact ccgcgttcag
     4321 ccagcatctc ctgcagctca cggtaactga tgccgtattt gcagtaccag cgtacggccc
     4381 acagaatgat gtcacgctga aaatgccggc ctttgaatgg gttcatgtgc agctccatca
     4441 gcaaaagggg atgataagtt tatcaccacc gactatttgc aacagtgccg gttatctcac
     4501 ccgaaataat tctcgcttta accgactcgt agtgggggct tccactggga tctatacctt
     4561 ccagtctgag gcttgcctca gccttcatga agccacgccg gcgctgttct tcactcatat
     4621 ctacctcata tgtcaatatc aggcgtacct catatgtcat gaaataatgt taaccagtac
     4681 aatgcattca gcccgaaaag acgcctgatc agtctggccg ggtcaggtat gcagcatggt
     4741 tggcgctgtg ttgcataccg ctaacgccca cggccaccac gttcattctg cttatccctc
     4801 tcgacttttg ccttaaactt ctccagaacc tgatcacgct tgctgagttt ctctttctcc
     4861 ccggactcca gttcttttgt ctcttcgctg agcgtttcat tctgcagtga agcctgatga
     4921 gacgcttttt ccagctctgc acgttcggct ttgtcggctt tatctctccg ggcatcacca
     4981 cgttgtttga gttcttcgat agtttccagc ttcatatcag ctttctcctg tttgcgggac
     5041 tgtacgcggg tacggtatgc agccatatct tccgcgttct tctgagcatt gagcgatttt
     5101 atctgcccag ccaagatcgg attttctttc gcggccagct ggtcggccag ccgttctccc
     5161 ggcgtagaca tttcataagc gccagactta cgcatgtagt ctctgacaaa gccaagtcga
     5221 tttttcagct gcaccagacg acgatcgagg ccatttttga tggtctgcca ggtactggcg
     5281 gcctgttcat aggcgctgcg tttaaacatt cctatcaggc cgctgggctg ttctggtctt
     5341 tgtcgctcat gtgaggatat tttttgtctc tggcgttcaa catgtgccgc cccacgggcg
     5401 gcaatccgtc cggccttatc ctgaacgaaa gtcagcttat cccggcgtaa actctgccat
     5461 tcaagccgga taacttccgg ccgttttgac ttgctggtta cagtgttttc ccgtcgcaga
     5521 ggctgatttc tgtcggttct ctggtgctcc acgtcacgag atacattgcc cggcaataac
     5581 atctcacttc gttctccgaa gcgaaccata tcgagttcgg acaggttgcg caggcgaccc
     5641 cttgcgctgg gcggcgggac ggctccaacg cggccagtgt aggactgagc ggtgcggact
     5701 cctcgcttat ctcgcccttt gtctcgttca gggactggag caatgcttcc atgtcctgct
     5761 ctgctgttgt tgtcatgggt tgccctccag ttatcaatca gctgaattgc ccgtgaaagc
     5821 ggctggattt cagcattacg caatataatc tggcgatatt cattgcctcg ttcggtctct
     5881 aagccgcgct tttccatctg cgcagctgcg taaccgatat gaatagtcgg aacccgatcc
     5941 agctttgctg ccttggctga atccccctga gaaatagctg attcccgttg cgcctcaagt
     6001 gaacgatggt caatacgctc tgaacggcca gccttttcca gatgacggtt cgtcatttct
     6061 gaccatgcag aacgccagtg gttgacttcg ccgctcttaa aatcatcaag ttcacgggtc
     6121 ttatccttga aaccttcctc cgacagtcgg cgggtgctgc ataaaacgtg agcatgatga
     6181 ttacgttggt ctccctcttt acccggagca tggatagcgg catccactgc cattccgtga
     6241 cgctcaacca gcgaagaaca aaaatcacga agcagctcaa gacgctgttt tccgtcaagc
     6301 tctgcaggaa atgcgagttc aaattctctg gcaacggttg aattacgacg gttttctttc
     6361 tgttcaactg cattccatag agtctcccga ttactcgccc aatctggtga gttttgaggg
     6421 agaaaaatct ctgaatgctc gacgcctgat ttacgggtgt agtcgtggat aatcccagtc
     6481 cgttcatcaa caattcttga acctgtacga taagcaattg ccgcagtggc cgaacggcca
     6541 gcactacggc tgattgtctt aacgctaaga tgaaaaattg ccaatttcgt attcctgttt
     6601 ttccgttgac cttgggtgca gggttttaac cctgccgcac gcaaactcga agagtttgca
     6661 taagtgcgcc ctttgctttc gcttcgggtt gaaatatatc agaaatgggg ttatcgttca
     6721 aatttgaatc aacgaggtga ctaatggcaa aatcaagagc ggaaaaattg gaagagttgc
     6781 gtcagaagga agctcagata aaggcgcaaa tccagcaatt aaagcaaaga gagcagacag
     6841 aagaacgcaa gaaggatacc cgccgaaaaa tcctaatcgg tggagccgta ctagcaaaaa
     6901 taaagcgtgg ggaatggaat tatcagcagt tggtcagtct tctggacaat gagctgaaaa
     6961 atgacagaga ccgggcactg tttgatgaaa tgggtttaaa ggcagttgac gccagaaaag
     7021 aatcttttaa ccccggtaat aagtgacgcc aattacttat taccggatga taaatcagat
     7081 caggagattt agcgtgaaaa aaaatatact ggccgggatt ttcttcgtcg caatgctatt
     7141 gccagtatgg cacgcagcgg ccgaaacatt cacccgctgc catccaaatg agacagtctg
     7201 ggcaatgatc caagatgagc agctaaaaaa catcgccaga ctacaggaaa caaagcggtg
     7261 tcaggccgtt aatgacactt atgaccagtg gcgccaaaat ttccaggcga tgggagatgc
     7321 agaaaacgcc atccgcagcg cccgcgatca tcagaactgg aacgccgtaa aatccacgtt
     7381 cgataacatt gctccccaga ttaatagcct gcatgcactg gcagaaaaaa attcatcgtt
     7441 actgggcgcg ggtaacatcc tggcgctttt cggcggtgat ctgaattacc tgtaccagaa
     7501 tgcagggctc gttgctccgg ccagcgctga cggactgact gataacgtca gtcaaatgct
     7561 gactgaaccg gataaacagg tcaaagctgc aggtatcgcg caacagctgg tttatcagac
     7621 tcgggttcgg gcacagagct ttatccaagg cgtggcacag aacgccggta acgaagccca
     7681 gaaagagtat cgtgcgcaat cagataagat tgtcgaagat aaagcccata atttcaaagg
     7741 tgacagcgtg gaaggatatt tcggcggctt ccttgcccgg ataagccagg tatatgctcc
     7801 agttctgctg ttccttatca tcgttggtat tattggtgca cttaccgcac ggcgggtaag
     7861 gctggatgct ttcacggcat cagtgaaagc agtcatggcc gcagcggtac ccgcagtggc
     7921 actggcgctg atgctgatat tcatgccttt cctaccatca tgggtattgt acgcggcagc
     7981 gctggccgta acattcgcca tttattcccg cagcaaaaaa attcttggag ggctggcctc
     8041 tcgctgtggg cgtttccccg cactggcctc cgggcttcgc tggatgggcg gttttgtgtc
     8101 tggcctcgat gctgacagaa agcaggaaac cggcgcggca tccatcaacc cggcatcaca
     8161 tcacactcac ggctcatcac gctggggaac cgctgcagaa atgcgggaaa ataaccacct
     8221 gttaccaacg gaaaagcaag ccggattcac cctggcacga gaactgtccc caccgcccgg
     8281 agtggatgga aaattctgtt tcaccgggca cgttgtgact gttgccccta cgggcagcgg
     8341 aaaagggatt ggcgcagtaa ttcccaatct tctgacttat cagggttcag cacttgtctt
     8401 tgatccaaaa ggggaaaacg ccgaggtgac aggtcgtcgc cgtcgtgaaa tgggtcagag
     8461 cgtccatgta atagacccat tcggtataac cagcggcata aaagaatcag tgaacattct
     8521 ggatcgtctc gatgtgacca gcccggattg cgtcgctgaa tctgcagttc tggccgaaag
     8581 ccttgtggta tcagagaaag gcggtgacgg taatcatttt gatgaatcag cccgaaccct
     8641 gttacagggc ctgatgctgc atgtttccgg attaccggat gagtcccgac gtaatctcgc
     8701 ggaactacgt cgactgatca ccagtgatga agaaaacctg ttagccctgc tggccgatat
     8761 ggccgcaagt gaagacgttg ctttcggcat ccccgcccga gcggccaaca cgctcatggg
     8821 gatggcagac cgggaacgtg gctcagtatt gtctaccgcc cgtaaaaaca cagcatttct
     8881 ggacgacccg cgtatagcag ctgcgctgtc acactcagac ttcaacctgt cagccatcaa
     8941 agctgaaccg atgacagttt atcttgtgct accagctaat cgcattgcag caaatacccg
     9001 atttgtaagg ctgtttatcg gttctgtgat ctccgctgta acagaatcga aggcgaagcc
     9061 agctcataat gtcgcattca tcctcgatga gttcaacgtg ctgggctata tgaaatcaat
     9121 cgaggatgcc gtaagcctgt tacgtggcta cgggctgtct ttctgggtct tcctgcagga
     9181 tctgtcacag ctgaaaagtg cctaccctaa atggcagacg tttctggcca actcagcgaa
     9241 gacgttctac ggcacagacg actatgacac tgcaaaatac gtcagcgaca ccttgggtaa
     9301 atcgacggtt gaatttgaaa cccagaattc aggccgcaac tctggcggcg gagtttccgg
     9361 gggtggcggc tcaatgaatc gtggccgttc atctggctca agtcagcagt ttgctgggag
     9421 ggaattactg acacctgacg aagtaatgag gcttggacca acccgaccaa ttgtgattct
     9481 gaaaggggaa tacccttatc agctcacacg tattaactat ctgacagacc cagaatacac
     9541 gggtatggca gaccctaacc cctactacag cgcatgatat ttggaatcgt tgtcggcgtt
     9601 ctgttagctc tgatgtttgg ccgggtcttg ctcccggcct taaccgccgt cgcctt
//
