LOCUS       NZ_CP103726             2495 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain 3131 plasmid pMB3093_4, complete
            sequence.
ACCESSION   NZ_CP103726
VERSION     NZ_CP103726.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28174019
            Assembly: GCF_024918795.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 135x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024918795.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:51:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,810
            Genes (coding)                    :: 4,691
            CDSs (with protein)               :: 4,691
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 44 of 119
            Pseudo Genes (incomplete)         :: 81 of 119
            Pseudo Genes (internal stop)      :: 13 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            Pseudo Genes (short protein)      :: 1 of 119
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103726.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="3131"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pMB3093_4"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2016-12-25"
                     /collected_by="MDACC"
                     /genotype="ST461"
     gene            129..347
                     /locus_tag="M5R19_RS24585"
                     /old_locus_tag="M5R19_24585"
     CDS             129..347
                     /locus_tag="M5R19_RS24585"
                     /old_locus_tag="M5R19_24585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            complement(638..823)
                     /locus_tag="M5R19_RS24590"
                     /old_locus_tag="M5R19_24590"
     CDS             complement(638..823)
                     /locus_tag="M5R19_RS24590"
                     /old_locus_tag="M5R19_24590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1189..1416)
                     /locus_tag="M5R19_RS24595"
                     /old_locus_tag="M5R19_24595"
     CDS             complement(1189..1416)
                     /locus_tag="M5R19_RS24595"
                     /old_locus_tag="M5R19_24595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          504..591
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(1832..2360)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 cgctgcgccc ggaacccgcc tgtaccggtt cagcagccgt tccggcctga ctgcaatttt
       61 ttttttttca tccctgcccg ctaccctgta aacctttctt ctgcgttgcc gttaacagta
      121 ggtaagccat gtttggtttt tcttccggag ccctgatgac tcttagtgat gatgagcgac
      181 acctgcttgt gtccgttgtt tcggtctggc tacggcgtgc cggaggggat gccggagcca
      241 tgatgctgga tgcataccgg cagattttgt ctgaaaccga accggccgta aggactgtca
      301 tgctcgaatt tcttgaatcc gttcgcattc attacatttc gtcctgagca ttttctgtat
      361 ttctgtccat acttaaacga ggcggccgtc acggacggtg gtcgtcgttc catgatgctg
      421 ttatggacga gtgagagtaa tctaatctaa cttgattgag ttccgggcgt gccgcccggt
      481 tttttttgct gtttcccggt gtccgttccg ggacaggatg tgcatttgta ataccgcgca
      541 cgcgccggca tcacaaatac cctggtcagg gctgcgcccc gacaccccgc catcgcctca
      601 cggccgcgca ctgaattgcg cacccgttca ccgattttca cgcattttcc tcgcctatct
      661 gtgcgttcag catggcgtaa agcgcttcgg cctgatcgtg caggtgctca cagcagccgg
      721 cagcctcatc cagatccatc tgctcgagcc gctcaaggag gagaagtgtc tgtgcgcgga
      781 taaatttggc catattgagg gcggcggatt cctgtctggt cactggtgat tttccccgtc
      841 tggtgatgat ttttgcgtga aaagagcatg gcgtcattct ggtgcatagt catgcatgcc
      901 gttaaaattt aacaggcgcg tttctgacgg gttccggggt ggtttgttgt ggtttttgtc
      961 atggtttcgt caggaacgcg ctgaacgcgt ctcaggcggt gcgggcaacg gatgttatgg
     1021 taaattcttg ggggcttgat tataaaatac cgcacttcct cttatgtctt gtgcttttta
     1081 gacaaccctg aaagctttta gctttggtga tgcaatttgc tgggattttg gcggagaacc
     1141 acaggtaaag aaaaaggcca cattagcggc ctttttcgga gaagatgctc agtgcggaat
     1201 ttcggagact tctttaacgt cggttttatt gatctgctgt ttgacaccat tcgcgtctgt
     1261 atagccaagc aggcctgagt ctgattcttt aggtttgccg tcgctgacaa tagtgcgacc
     1321 gtcattggtg tgtatggcat agcttgtgcg cgtgcagccg gtgagggctg aaagcgcaac
     1381 tgccgctgcg aacactgaaa caaaaatctt tttcaaagcc agctcctttt atccatatca
     1441 gttttggtca ctaaccatct aactataggt cattttttat gtgaaaagag gcttgatggt
     1501 gggctgtata tcgagttagg cgcgaggtgc tatggtcaaa gtgtggtgtc aggcggggag
     1561 gaggcttata tgagatgtaa tccggccgat acatatacca agatgattta agtattcctc
     1621 tttgtgaggt tggatgtcta atttataaag gatcttcttg agatcctttt tttccgcgcg
     1681 taatctcttg ccctgtaaac gaaaaaacca ccctggcagg tggtttttcg aaggttaggt
     1741 aatcctggca gatcccctaa ccgtggtaac agtcttgtgc gagacatgtc accaaatttg
     1801 tcctttcagt gtagcctcac taaggccgcc acttcaagaa ctcttgagac atctctcgca
     1861 catcctgttt gccaatggcc gttgccaatg gcgattagtc gtgtctttcc gggttggact
     1921 caagttgata gttaccggat aaggcgcagc ggtcggactg aacggggggt tcgtgcatac
     1981 agtccatcct ggagcgaact gccttcccgg aactgagtgt caggcgtgga atgagaaacc
     2041 gcggccataa cagcggagtg acaccggtaa accgaaaggc aggaatgcgg gggagcacga
     2101 gggagccacc agggggaaac gcctggtatc tttaagccgc atcgggtttc gccaccactg
     2161 atttgagcgt cagattctgt gatgcttgtc aggggggcgg agcctatgga aaaacggctt
     2221 tggcgcggcc ttatgctttc ttcgttaagt atcttcctgg catcccccag gaaatttctg
     2281 atccatccgt aagcccgtcc cgctcgccgc agccgaacga ccgagcggag cgagtcagtg
     2341 agcgaggaag cggaatatat tctgtatcac attttctcct gacgcgtttt ctttcacttt
     2401 ctgcgcctgt cttatgtggc attaatgcta tgtgttactg ccatgctaca tcttaagcca
     2461 gtatacactc cgctagtgct ccgtgactgg tccgg
//
