LOCUS       NZ_CP103725             3223 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain 3131 plasmid pMB3093_3, complete
            sequence.
ACCESSION   NZ_CP103725
VERSION     NZ_CP103725.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28174019
            Assembly: GCF_024918795.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 135x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024918795.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:51:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,810
            Genes (coding)                    :: 4,691
            CDSs (with protein)               :: 4,691
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 44 of 119
            Pseudo Genes (incomplete)         :: 81 of 119
            Pseudo Genes (internal stop)      :: 13 of 119
            Pseudo Genes (multiple problems)  :: 17 of 119
            Pseudo Genes (short protein)      :: 1 of 119
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103725.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="3131"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pMB3093_3"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2016-12-25"
                     /collected_by="MDACC"
                     /genotype="ST461"
     gene            complement(join(3058..3223,1..167))
                     /locus_tag="M5R19_RS24555"
                     /old_locus_tag="M5R19_24555"
     CDS             complement(join(3058..3223,1..167))
                     /locus_tag="M5R19_RS24555"
                     /old_locus_tag="M5R19_24555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_001099531.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     gene            complement(335..550)
                     /locus_tag="M5R19_RS24560"
                     /old_locus_tag="M5R19_24560"
     CDS             complement(335..550)
                     /locus_tag="M5R19_RS24560"
                     /old_locus_tag="M5R19_24560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(554..1039)
                     /locus_tag="M5R19_RS24565"
                     /old_locus_tag="M5R19_24565"
     CDS             complement(554..1039)
                     /locus_tag="M5R19_RS24565"
                     /old_locus_tag="M5R19_24565"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_025367738.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<1074..1778)
                     /locus_tag="M5R19_RS24650"
                     /pseudo=""
     CDS             complement(<1074..1778)
                     /locus_tag="M5R19_RS24650"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1717..2040)
                     /locus_tag="M5R19_RS24575"
                     /old_locus_tag="M5R19_24575"
     CDS             complement(1717..2040)
                     /locus_tag="M5R19_RS24575"
                     /old_locus_tag="M5R19_24575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2679..2849
                     /locus_tag="M5R19_RS24580"
                     /old_locus_tag="M5R19_24580"
     CDS             2679..2849
                     /locus_tag="M5R19_RS24580"
                     /old_locus_tag="M5R19_24580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004193911.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNWVHGAEIV
                     ENAYRVLEVKA"
                     /name="hypothetical protein"
     Typing          complement(2137..2328)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.821"
                     /coverage="89.0"
                     /evalue="7.28e-73"
     Typing          2308..2791
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.756"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          complement(238..1727)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.784"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtaggtatat tttccaatgt ttgggaaagc agataaaagg cagcgcaata acttattgca
       61 gttcctacag acggccacag gcgtgtaaat ccttctgaat atttcataaa ggtggtgcct
      121 atgacttcag ccacaatagc ccctcccagg aaaatatatg tattcataaa aatacctcat
      181 gttgaaaaat ttaaatatct ttacataaca actaattaat agaacccggc ttatcatagc
      241 gaaggaccat gatgctgcca ctgcgatcgt tccagctcca gcgctttctg gtgctgcacc
      301 gcccgctcct gctgccggtg agtttctatc gcgttcagcc tttcgacaac cggcgcagct
      361 gctcgctcaa actctgcacc tgcctgctca agaccgtgac gcgctcgttc agcgccgcgt
      421 tctcccgttg aataagacca gacatcgtcc gccattccgc gaaggcgttc tcccactcgt
      481 ccagcctttt cgagtagtcc tgctgtagct gctctaatgc gctcagcaac tgtttttcca
      541 gctccgtcat gtgctatttc cccgccagta tcatccagct cgagtcctct ccgaactgtc
      601 ccgcttccgg gttcaccctc acgcagcggc gtctctgctc gccgcaggtc gagagctgta
      661 cgccgctgtt cctctctgac agcatggcct gcgtgctctt ctgctcccgg atggtcgtat
      721 agttctcgag tatctgctgc ccctgccacc acagaatgcc cgcgctcgac gctatcagca
      781 gcgcggagac cagaacgatg gtcagccacg tctggctgac catgcgcagc atccctttcg
      841 tgcgctggct catggctgag gacagcttcc ggtcgtggtc gaggatggcg gcgctgattc
      901 tcttctcgct ttcgctcagt tccgctctga cagacttctc gtgctcgctg aatgcggctt
      961 tcagcatctc gccggtagtc tgctgctgcg ctttcgattt ctgctctaag tccttcgcca
     1021 gcgttaaaag actgttcata gatggctccc ttcagtcgga tgtttcgccc cccgtccggg
     1081 tcggcaatgc tgatgctgct ttttgtggtg cgcaccacct caaaacctgc gctttccagc
     1141 gcctcagtga cgtcctgacg cgttttcagc tccccggatg aggcaagggc aagcaggccg
     1201 ctcgtaatcg cctgggcggc ttcctgcttc gcttccggca gcgcggaagg cgagaccagc
     1261 acccgccggt tctccggcgc gttcgggtca tgcagcccca gcctgccgtt cactatggtc
     1321 tgccaggcat cgatgcgcgg acggtcggcg cggtcgtaat acggctgtag ccgcttgccg
     1381 gtcagaagct ccgtgttcgg gatcaggaag ttcagctcca gccgcccctt gtcccggtgc
     1441 tcaacccaca gcacgctgta ctggtcttta tcgagtccgg gcatcagaac ccgctcgaaa
     1501 ctcgccatca gcttttcgcg ctggccgggc ggtaaatcct gttcggcaaa agacagaacg
     1561 ccggaagtgt actttttggc gtagggcgag gcgtcgataa gctcccggac ttcatccggc
     1621 ttcccctgca gaacgctggc gccgtcgcgc tggcgatctt tacccagcag ataatcgacc
     1681 ggcccgccgc cgccgccgcg cccccgagga tgaaacttaa cgatcatcat cagcaccctt
     1741 ttccagtacg gcatgccgca accgctcgag tccggcatcg atggccatca gcgcggcgac
     1801 aatctgcacg cggtcgtgtc cgcttccacc accggcgtta acctggcggg caatctggtt
     1861 aaggttgttc cccatgccgg caagctgacg aagcagcgcc ggcgagatcg acggaagttt
     1921 gccggcgcgt gcgggcttct cgtccaggca cgtctgccgc atccaggccg cgagttgctt
     1981 accctcgcag cgctccagca gacgccggtg ctcgtcctca gtcacccaca tggtgagcat
     2041 tttgctgcgt ttgtctgcca atccggcctc ctgataacgc cgggacggac gggaaatctt
     2101 ttcccggtcg gcacggtgtt ggacaagccg cagacgcggc agtggtttta gcgggtttcg
     2161 gggcgcagcc ctgaaccagt cacgtagcgc tagcggagtg tatactggct aaacatgtta
     2221 tgacggagtg gtaatttatg aaagtgcttc atgtgacagg ggaaaaatgc ggctgccggg
     2281 cgtaagtgca aaatgcaggt ggtgatgaaa tgacgcctcc tcgctcactc ggtcgctacg
     2341 ctcctgccgt gagactgcgg cgggcgttac cagctcacag attacgggat aagcaaatgc
     2401 cgctcgaagc aggaaaggcc aagaaaagga tgcggagttg ccgtttttcc ataggctccg
     2461 cccccctgac gagtatcacg aaaatgacgc tcaaatcagc ggtggcgaaa cccgatgcgg
     2521 cttaaagata ccaggcgttt ccccctggtg gctccctcgt gctcccccgc attcctgcct
     2581 ttcggtttac cggtgtcatt ccgctgttat ggccgcggtt tctcattcca cgcctgaaac
     2641 tcggttccgg gtaggcagtt cgctccagga tggactgtat gcacgaaccc cccgttcagt
     2701 ccgactacca cgcccgttcc ggtaactatc aacttgagtc caacccggaa agacacgaca
     2761 aatcgccagt ggcggtagcc attggtaact gggttcacgg agcagagatt gtagagaatg
     2821 cctacagagt tcttgaagtg aaagcctgag tgcggctaca ctgaaaggac agtttaggta
     2881 tctgcgctcc gtgaaagcca gttaccatcg gaacaaagag ttggtagctc tgaattccgg
     2941 caaacgaacc accgttggta gcggtggttt ttttgtttac agggaaaaag attacgcgca
     3001 gaaagaaagg atctcaagga aatccttttg ctttggccaa tatgcttcgg taaaaagtta
     3061 atgcgcagaa gattttgaaa aaatattaat aaccaaaact ccaacgcaaa taaggaacat
     3121 tcctgcaatt gcgggccaat caagtttttg accgctaacc aaccaaccag ctaggcttat
     3181 tagtacaatt cccgcaccag accaaattgc gtaagcaata ccg
//
