LOCUS       NZ_CP103707             7939 bp    DNA     circular CON 21-SEP-2023
DEFINITION  Escherichia coli strain ST361 plasmid pMb3362_3, complete sequence.
ACCESSION   NZ_CP103707
VERSION     NZ_CP103707.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28174001
            Assembly: GCF_024918335.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 7939)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 48x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/21/2023 03:32:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,004
            CDSs (total)                      :: 4,884
            Genes (coding)                    :: 4,624
            CDSs (with protein)               :: 4,624
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 84 of 260
            Pseudo Genes (incomplete)         :: 163 of 260
            Pseudo Genes (internal stop)      :: 49 of 260
            Pseudo Genes (multiple problems)  :: 34 of 260
            Pseudo Genes (short protein)      :: 4 of 260
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103707.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ST361"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMb3362_3"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2017-02-23"
                     /collected_by="MDACC"
                     /genotype="ST361"
     gene            1..192
                     /locus_tag="M5T41_RS24805"
                     /old_locus_tag="M5T41_24805"
     CDS             1..192
                     /locus_tag="M5T41_RS24805"
                     /old_locus_tag="M5T41_24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            851..1009
                     /locus_tag="M5T41_RS25050"
     CDS             851..1009
                     /locus_tag="M5T41_RS25050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071529495.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAATGNWTRWIEIL
                     RVLEVVA"
                     /name="replication initiation protein"
     gene            1557..2255
                     /locus_tag="M5T41_RS24810"
                     /old_locus_tag="M5T41_24810"
     CDS             1557..2255
                     /locus_tag="M5T41_RS24810"
                     /old_locus_tag="M5T41_24810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000939065.1"
                     /translation="MLQHKMNSSSYAKVHNVSSLEDIMSYHNDDVLLKFRKEWNVTPEE
                     ADDIFNETKKFIWLASTCLTECYNIKVHEQLQIIDEMWHTFIQFTDAYTSFCEKYLGAY
                     LHHYPNTNDMLKNEIRHVNEHGITFQEYRFNEYKNQIEKIAFYLGHETVAKWYGDYAVR
                     YSIKNINTIRIPKESISSDSYIEKVKSITHLPAAEFVKIIMRKDVWNDNGSVCGCSGKG
                     CGAGCSCNSR"
                     /name="hypothetical protein"
     gene            2294..3886
                     /locus_tag="M5T41_RS24815"
                     /old_locus_tag="M5T41_24815"
     CDS             2294..3886
                     /locus_tag="M5T41_RS24815"
                     /old_locus_tag="M5T41_24815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842142.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_001009226.1"
                     /translation="MNFIEKYIITFNKCNLFFIILLSTISSFLFVLFGYLIKTVIDNKD
                     SIGEINSLLIFIACFLSIRFLMPAGYSISEYLTQKTNIELSVKLREQVIDNIQNSHQEH
                     FLKKNKGELNKVIESMLSSASSLFYTICSDVVPLLIQMIGIIITICISVNTLIAIEFII
                     IMAIYIIFVIKMTQRRFPMMKSVALSSKFASGRMFDMMHMYPMDKAFHTTDKSRKRVIQ
                     AVNTHSEKQRKVNNEFFLFGISSAFLSVIFSSLIILSAYWMFLHGRASRGSIIMLATFL
                     FQVFLPLNRIGYLFRQIKMARTEIDLYCAEINDIKKTNYTDKQYLHVKDNICNIEIYNK
                     SFNHKLILTKGVVTFITGENGSGKTTIAKILSGNITTEENTIKINGIQQEKANSPFVNV
                     LYVPQDLDLMPGTIEENILHYSGINNLNTIKKLLHRFKFDKPLDYEIKGYGHNLSGGQK
                     QKLGVSLTSGKNVDLIIFDEPTKGFDSLGIKIISDYIKEENKEKHIIVISHDEQLINNI
                     PKAQIIDITKHEV"
                     /name="ABC transporter ATP-binding protein"
     gene            3889..4782
                     /locus_tag="M5T41_RS24820"
                     /old_locus_tag="M5T41_24820"
     CDS             3889..4782
                     /locus_tag="M5T41_RS24820"
                     /old_locus_tag="M5T41_24820"
                     /inference="COORDINATES: protein motif:HMM:NF025007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="WP_001061104.1"
                     /translation="MNNLIKKEIIEKFKKYNFQKYPFVFTDVNYKNLINWNDLNKLLEK
                     DILHYPRVRMANDNFPEIRGYKGFIRYTYSQTGDRTPHINRHQLYKCLRDGATLIVDRC
                     QSFFESVDESRLWLSKELECTCSANLYAAFTATPSFGLHFDNHDVIAVQIEGIKKWKVY
                     NPTYSYPLEDERSFDYLPPNTSPDYEFDITPGQAIYLPAGYWHNVTTQSKHSLHISFTV
                     IRPRRLELFKTLFDELKNNPYMREPIEHGDSLSDKEKIKTIITNAINSFDIQFAENMLK
                     AQSRTYRYKKIKLEHI"
                     /name="cupin domain-containing protein"
     gene            complement(4807..5169)
                     /locus_tag="M5T41_RS24825"
                     /old_locus_tag="M5T41_24825"
     CDS             complement(4807..5169)
                     /locus_tag="M5T41_RS24825"
                     /old_locus_tag="M5T41_24825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="WP_000181070.1"
                     /translation="MTNFKSDLNLIRKEWYNAFYSGNTEMLDYLEADWFISTNGQKVIY
                     KKHQLRKIDMRPARILPSIERYEYDVVMREFKGIACVSGKAEIKQNDGNILMAFIENWI
                     KVNGGWKMQFISYEKI"
                     /name="DUF4440 domain-containing protein"
     gene            complement(5166..5405)
                     /locus_tag="M5T41_RS24830"
                     /old_locus_tag="M5T41_24830"
     CDS             complement(5166..5405)
                     /locus_tag="M5T41_RS24830"
                     /old_locus_tag="M5T41_24830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 family protein"
                     /protein_id="WP_000703021.1"
                     /translation="MKISDIEKNIYKGLAEFISQKIRDEHDICLGQFESEELLDEILKR
                     VIPVIYNVAIDDAIKSVRNVGDRLEEELDMRKVV"
                     /name="DUF2164 family protein"
     gene            complement(6116..6349)
                     /locus_tag="M5T41_RS24835"
                     /old_locus_tag="M5T41_24835"
     CDS             complement(6116..6349)
                     /locus_tag="M5T41_RS24835"
                     /old_locus_tag="M5T41_24835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137439.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(6356..6874)
                     /locus_tag="M5T41_RS24840"
                     /old_locus_tag="M5T41_24840"
     CDS             complement(6356..6874)
                     /locus_tag="M5T41_RS24840"
                     /old_locus_tag="M5T41_24840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673203.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVLFLFTVSG
                     GTLWYLGGMIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            complement(<6912..7562)
                     /locus_tag="M5T41_RS24845"
                     /old_locus_tag="M5T41_24845"
                     /pseudo=""
     CDS             complement(<6912..7562)
                     /locus_tag="M5T41_RS24845"
                     /old_locus_tag="M5T41_24845"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(7552..7875)
                     /locus_tag="M5T41_RS24850"
                     /old_locus_tag="M5T41_24850"
     CDS             complement(7552..7875)
                     /locus_tag="M5T41_RS24850"
                     /old_locus_tag="M5T41_24850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955993.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVLAALMAIGEELRRLRLAVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(269..348)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.78"
                     /coverage="92.0"
                     /evalue="8.94e-33"
     Typing          381..923
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.679"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(6013..7562)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.787"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7559..7908)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.872"
                     /coverage="99.0"
                     /evalue="9.42e-67"
ORIGIN
        1 gtgactaaac aggaaaaaac cgcccttaac atggcccgtt ttatcagaag ccagacatta
       61 acgctgctgg agaaactcaa cgaactggac gcagatgaac aggccgatat ttgtgaatca
      121 cttcacgacc acgccgacga gctttaccgc agctgcctcg cacgcttcgg ggatgacggt
      181 gaaaacctct gacacataca gctcccggag acggtaacag cttgtctgtg agcggatgcc
      241 gggagcagac aagcccctca gggcgcgtca gggggtttcg gggcgaagcc ctgaaccagt
      301 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagtac gaattgtatg
      361 gaaagcgcac catgtccgat gtgaaatgcc gcacagatgc gtaaggagaa aatgcacgtt
      421 ccggcgctct tccgcttcct cgctcactga ctcgctacac tcggtcgttc ggctgcggcg
      481 agcggtgtct gctcactcaa aagcggagat gcggttatcc acagaatcag gggataacgc
      541 caaaagaaac atgtgagcaa aaaacgagaa ccaggaaaag gctgcgccgt tggcgttttt
      601 ccataggctc cgcccccctg acgagcatca taaaaatcga cgctcaagtc agaggtggcg
      661 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
      721 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
      781 ggcgctttct catagctcac gctgtaggta tctcggctcg gtgtaggtcg ttcgctccaa
      841 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
      901 tcgtcttgag tccaacccgg taagacacga caaagcgcca ctggcagcag ccactggtaa
      961 ctggacaagg tggattgaga tattaagagt tcttgaagtg gtggcctaac tgcggctaca
     1021 ctagaaggac agtatttggt atctgtgctc caccaagcca gttacccggt taagcagtcc
     1081 ccaactgact taaccttcga ctaaaccgcc tccccaggcg gttttttcgt ttactggcag
     1141 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttt gagatccgcc
     1201 cactcctgga acctacaaca catgtgtaaa acgtcaatgg agggggctat tatcggactg
     1261 caacacattt cgtacagcct ttacactcgg tgaattagcg gccctagatg catccactca
     1321 ttcaaccaac tcaattctct tctcttagag tgggaaaatt ttgttttcct tgcagcccct
     1381 acccgtaaaa actggctgca aaaaccttgt attactaatg gatcctataa ccattaaaaa
     1441 acttgattac tatctcattt atagaattag gcatattgac acaagcacaa atttaacaat
     1501 accataacaa atgataacta ttctcatcta cattcaaata tataattggg ggtgttatgc
     1561 tacaacataa gatgaacagc agttcttatg caaaagttca taatgttagc tcattagaag
     1621 atatcatgag ttatcacaat gatgatgttc ttctaaaatt tcgtaaagaa tggaatgtaa
     1681 caccagaaga agctgatgat atctttaatg aaacaaagaa attcatctgg ctagcctcaa
     1741 catgcctaac ggaatgctac aatataaaag ttcacgagca attacaaatt atagatgaaa
     1801 tgtggcatac gtttattcaa tttacagatg cttataccag cttttgtgaa aaatatcttg
     1861 gtgcttacct ccaccattat ccaaatacaa atgacatgct aaaaaatgag ataaggcatg
     1921 ttaatgagca tggtataaca ttccaagaat atcgttttaa cgaatataaa aatcaaattg
     1981 aaaaaatcgc tttttaccta ggtcatgaaa ctgtcgcaaa gtggtatggt gattatgctg
     2041 taagatacag cataaagaac attaatacta taagaattcc aaaagaatcc atatccagtg
     2101 attcttacat cgaaaaagta aagagtatca ctcaccttcc agccgcagaa tttgtaaaaa
     2161 taataatgcg aaaagatgtt tggaatgata atggttctgt ttgtggttgc agcggtaaag
     2221 gatgtggcgc tgggtgctca tgtaattcta gataacataa agcccgtaat atacgggctt
     2281 taaggattat aaattgaatt ttattgaaaa gtacatcatc acatttaata aatgtaattt
     2341 gttttttatt atattattat caactataag tagttttctt ttcgttctat tcggatattt
     2401 aataaaaaca gttattgaca ataaagattc aataggagaa ataaactcat tattaatatt
     2461 tatagcatgt ttcttatcaa ttagattcct tatgcctgct ggatatagca tatcggaata
     2521 cttgactcaa aaaacaaata tagaattatc tgtaaagctt agagagcaag ttattgataa
     2581 tatacaaaat tctcaccaag agcatttttt aaagaaaaat aaaggagaac taaataaggt
     2641 tatagaaagc atgctttctt ctgcatcatc attattttat acaatatgtt ctgatgtagt
     2701 accattatta atacaaatga ttggaataat cataacaatt tgtataagtg ttaatacatt
     2761 aatagccatt gagtttataa taataatggc aatctatata atatttgtca taaaaatgac
     2821 acaacgtaga tttccaatga tgaaatcagt tgcacttagc tccaaattcg catctggaag
     2881 aatgtttgat atgatgcaca tgtatcctat ggataaagct ttccatacca cggataaaag
     2941 tcgtaaacga gtaattcaag ctgtaaatac acattcagaa aaacaaagaa aagtaaataa
     3001 tgaatttttc ctgtttggta tcagctccgc ttttctttca gtcattttta gtagtctgat
     3061 tatcctatca gcatactgga tgtttcttca tggtagagca agccgtggta gtattattat
     3121 gcttgccact tttttattcc aagtttttct cccattaaat cgcattggtt atctatttag
     3181 acaaataaaa atggcaagaa cagaaattga tttatactgt gccgaaataa acgacataaa
     3241 aaaaacaaat tacacagaca agcaatatct tcatgtaaaa gacaatattt gcaatattga
     3301 aatatacaac aaatcattca accataaatt aatattaaca aaaggcgttg ttacctttat
     3361 aaccggtgag aatggttcgg gaaaaacaac tattgcaaaa attctctctg gcaatataac
     3421 aacagaagaa aacacaataa aaatcaatgg gatacaacaa gaaaaagcaa attctccttt
     3481 tgttaatgtc ttatacgttc ctcaagatct agatctcatg cctggaacta tagaggaaaa
     3541 tatattacat tattccggca ttaataattt aaatacaatt aaaaagctgc tacacagatt
     3601 taagttcgac aaaccactag attatgagat caaaggttac ggacataatt tatctggtgg
     3661 acaaaaacaa aaattaggag tatctcttac atctggaaaa aacgtagact tgattatttt
     3721 cgatgaaccg acaaaagggt ttgattcact tggtataaaa ataatcagtg attatataaa
     3781 agaggaaaac aaggaaaagc atatcattgt tatatctcat gatgaacaac ttataaataa
     3841 tatacctaaa gcacagatta tagatatcac aaaacatgag gtttaaaaat gaataatctt
     3901 ataaaaaagg aaatcataga aaaatttaag aaatataatt tccagaaata tccgtttgta
     3961 ttcacggatg ttaattataa aaacttaatc aactggaatg atcttaataa attgcttgaa
     4021 aaagatatat tgcactatcc tagagttaga atggcaaatg acaactttcc cgaaattagg
     4081 gggtataaag gatttataag atacacatat agccaaacag gcgatagaac accacatata
     4141 aatcgccatc agctatataa atgcttacgt gatggcgcaa cccttatagt agatcgatgc
     4201 cagtcattct ttgaatctgt agatgaaagc agactatggt tatctaaaga gttagaatgt
     4261 acatgtagtg ctaatctata tgctgcattt acagcaacac caagctttgg gcttcatttt
     4321 gacaatcatg atgtaatagc tgttcaaatt gagggaataa aaaaatggaa agtttacaac
     4381 cctacctatt cataccctct cgaagatgaa agaagcttcg attatctacc acctaatacc
     4441 tccccagatt atgagtttga tataacccca ggtcaggcca tatatcttcc tgctggatac
     4501 tggcacaatg ttacgactca gagcaaacac tctcttcata tatcttttac agttataaga
     4561 cctcgtcgat tagaactatt taaaacatta tttgatgaat taaaaaacaa tccatatatg
     4621 cgggaaccta tagagcatgg tgattcatta tctgataaag aaaaaataaa aactataata
     4681 actaatgcta taaatagttt tgatattcag tttgcagaaa acatgcttaa agctcaaagc
     4741 agaacttatc gctataaaaa aataaaactt gaacatatat aaccattaat ctaagggggc
     4801 ttccccttag attttttcat acgatataaa ctgcattttc catccaccat taacctttat
     4861 ccaattctca ataaaagcca ttaatatatt tccatcattt tgctttattt cagctttccc
     4921 tgaaacacaa gcaatgcctt taaactcacg cattacgaca tcatattcat atctttctat
     4981 gctaggtaaa atcctcgctg gacgcatatc aatctttcgt aactgatgtt ttttatagat
     5041 aactttttga ccatttgttg aaataaacca atcagcttcc aagtaatcta gcatttcagt
     5101 attacctgaa taaaatgcat tataccattc ttttcttatt agatttaagt ctgatttaaa
     5161 attagtcaca ctaccttcct catatctaat tcctcctcta atctatcccc aacattcctt
     5221 actgatttta tagcatcatc aatagccaca ttatatatga ctgggattac tcttttaaga
     5281 atctcatcaa gtaattcctc tgattcaaac tgtccaaggc atatatcatg ttcatctctg
     5341 attttttgtg aaataaattc agctagtcct ttatatatgt ttttttcaat atcagaaatt
     5401 ttcattcttt atgaagcctc acaaataaat taataatata acatttttcc tatcatagga
     5461 aaattacaac tcaaccatac tgcaacctgg aatttcccaa gcaagcatat ttaaataagt
     5521 gcaggatacc acctcaaatc attctctaag gaacaagata atttaccata atcccttatt
     5581 gtacgcaccg ctgaaacgcg ttcggcgcga tcacggcagc agacaggtaa aaatggcaac
     5641 aaaccacccg aaaaactgcc gcgatcgcac ccggtaaatt ttaaccacat gcatagctat
     5701 gcagccatgt gaatcacgca ggaattgcca gccagagaca gctgaaacgg atttttttca
     5761 tgctttcgga agcgaagaag acggggaccg gagcgggaaa acagaagttc aggggaatga
     5821 gcgcgatctg gcaatagagg caaaacacag caacaaaaga cacaccagaa tcgcgcccgt
     5881 atgcgtttta acgcgttcct gtgttttcag caaggtctga ctgaagcgcg ttcccggtgc
     5941 ccccgaacca accattatcc caaaaaatgc accggaaacg cgctgacaac attttagcct
     6001 gtctctgatt accatcccag cgaagggcca tcctgtgtcc gttcctgtat ttccagctgg
     6061 cgctcacgct ccagggataa ctcatgttcg cgttcctgta tcagccgttc atcccttatc
     6121 tcctgttctc gctgtataac tggctcaagc gttctgtctg ctcgctcaag tgctgcacct
     6181 gctgactcaa ctgcatgacc cgctcgttca gcatcgcgtt gtcccgttgc gtaagcgaaa
     6241 acattttctg caattccacg aaggcgctct cccattcgtt cagccgctgc atatagtcct
     6301 gttgcagttg ctctaaggcg ttcagcaaat gtctttccag ctccgtcact ctgtgtcact
     6361 ccgtcagttg tacccagtcc ttcccctgat agggaatcac cgttgccgat ttcccctgag
     6421 ggataaccag aaatttcctt ctcccgtcct gcacaaactc cacgccccat gtcttcgcgt
     6481 tcagtttctg caatgtttct tcctgtatcc tgatttcttc caggttcgcc tgtatcatcc
     6541 cgccaagata ccagagcgtc ccgccactca cggtaaacag gaaaaggacc atccccagta
     6601 acatcatgcc cgtattccct gccagcttta acacgtcctt cctgtgctgc atcatcgcct
     6661 ctttcacccc ttcccggtgt tttttcagtg attcctctgt cgaagctgtg aacagggcta
     6721 tagcgtcttt gattttcgtc tcgtttgatg tcacagcctt gcttacagat ttttcgagct
     6781 tgctgaactc gtcgttcagc attgtctctg tagattcggc tctctctttc agctttttct
     6841 cgaactccgc gcccgtctgt aaaagattgc tcataaaatg ctcctttcag cctgatattc
     6901 ttcccaccgt tcgggtctgc aatgctgata ctgcttcgca tcaccctgac cacttcaagc
     6961 cctgcctctg tgagcgcctg aatcacatcc tgacgacctt ttatctcccc gacatgataa
     7021 agagcgtcta tcccgcgtgt gacgctctct gcaagcgcct gtttcgtttc cggcaggtta
     7081 tcagggagag tcagtatccg gcggttctcc ggggcgttgg ggtcatgcag cccgtaatgg
     7141 tgattcacca gcgtctgcca ggcatcaatt ctcggcctgt ctgcccggtc gtagtacggc
     7201 tggaggcgtt ttccggtctg tagctccatg ttcggaatga caaaattcag ctcaagccgt
     7261 cccttgtcct ggtgctccac ccacaggatg ctgtactgat tcttttcgag accgggcatc
     7321 agtacacgct caaagctcgc catcaccttt tcacgtcctc ccggcggcag ctccttctcc
     7381 gcgaacgaca gaacacctga cgtgtatttc ttcgcaaatg gcgtggcatc gatgagttcc
     7441 cggacttctt ccggattacc ctgaagcacc gttgcccctt cccggtttcg ctcccttccc
     7501 agcaggtaat caaccggacc actgccaccg ccttttcccc tggcatgaaa tttaacaatc
     7561 atcccgcgcg ccctgttccc tgacggccag acgcagccga cgcagctcct ccccgatggc
     7621 catcagcgca gccaacacct gaacccgatc accggaagac cactgcccgc tgttcacctt
     7681 cctcgctgtc tgattcaggt tattcccgat ggcggccagc tgacgcagta acggcggtgc
     7741 cagtgtcggc agcttcccgg agcgtgcaac cggctcaccc aggcagaccc gccgcatcca
     7801 taccgccagc tgtttaccct cacagcgttc cagtaaccgg gcatgttcat catcagtaac
     7861 ccgtatggtg agcatcctct cgcgtttcat cggtatcatt accccataaa acagaaatcc
     7921 cccttacacg gaggcatca
//
