LOCUS       NZ_CP103682             4096 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain MB4528 Variant plasmid pMB4528_3,
            complete sequence.
ACCESSION   NZ_CP103682
VERSION     NZ_CP103682.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28174038
            Assembly: GCF_024918215.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 97x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024918215.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:56:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,018
            CDSs (total)                      :: 4,904
            Genes (coding)                    :: 4,787
            CDSs (with protein)               :: 4,787
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 40 of 117
            Pseudo Genes (incomplete)         :: 83 of 117
            Pseudo Genes (internal stop)      :: 12 of 117
            Pseudo Genes (multiple problems)  :: 17 of 117
            Pseudo Genes (short protein)      :: 1 of 117
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103682.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="MB4528 Variant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pMB4528_3"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2017-10-22"
                     /collected_by="MDACC"
                     /genotype="ST90"
     gene            1..324
                     /locus_tag="M5T06_RS25020"
                     /old_locus_tag="M5T06_25020"
     CDS             1..324
                     /locus_tag="M5T06_RS25020"
                     /old_locus_tag="M5T06_25020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001555769.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGSSGLDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            533..>967
                     /locus_tag="M5T06_RS25025"
                     /old_locus_tag="M5T06_25025"
                     /pseudo=""
     CDS             533..>967
                     /locus_tag="M5T06_RS25025"
                     /old_locus_tag="M5T06_25025"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1002..1487
                     /locus_tag="M5T06_RS25030"
                     /old_locus_tag="M5T06_25030"
     CDS             1002..1487
                     /locus_tag="M5T06_RS25030"
                     /old_locus_tag="M5T06_25030"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012782630.1"
                     /translation="MNSLLTLAKDLEQKSKVQQQTTGEMLKAAFSEHEKSVRAELNESE
                     KRISAAIHDHDRMLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLTTCGEERRRCVRVNPDAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            1491..1700
                     /locus_tag="M5T06_RS25035"
                     /old_locus_tag="M5T06_25035"
     CDS             1491..1700
                     /locus_tag="M5T06_RS25035"
                     /old_locus_tag="M5T06_25035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014538403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_063962836.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMCGLMQRENA
                     ALSERVTDLSMQVLSLSEQLRRLS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2230..2457)
                     /locus_tag="M5T06_RS25040"
                     /old_locus_tag="M5T06_25040"
     CDS             complement(2230..2457)
                     /locus_tag="M5T06_RS25040"
                     /old_locus_tag="M5T06_25040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_000719360.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPTES
                     DSGLLGYKDANGVKQQINKADVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            complement(2479..2712)
                     /locus_tag="M5T06_RS25045"
                     /old_locus_tag="M5T06_25045"
     CDS             complement(2479..2712)
                     /locus_tag="M5T06_RS25045"
                     /old_locus_tag="M5T06_25045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461706.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_001036746.1"
                     /translation="MNKKTLLAAMMVALVTFAAAGCSSNQEIKTTDDRTIVTDGKPKVD
                     SDTGLVSYKDAHTGRTEQINRDKITNMSELDN"
                     /name="YgdI/YgdR family lipoprotein"
     gene            2794..3063
                     /locus_tag="M5T06_RS25050"
                     /old_locus_tag="M5T06_25050"
     CDS             2794..3063
                     /locus_tag="M5T06_RS25050"
                     /old_locus_tag="M5T06_25050"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_079839079.1"
                     /translation="MNGQEFRLLLTEHGEKEAVLLFGKKSFVAVPDTQNLYGGCYYLYL
                     EDAHKNELLIELNESDLYPGGAIELQHNGGRKIHCEILKVRWSR"
                     /name="hypothetical protein"
     gene            complement(3300..3458)
                     /locus_tag="M5T06_RS25115"
     CDS             complement(3300..3458)
                     /locus_tag="M5T06_RS25115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032670878.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHEKSLLAAAISNWRYTRDKL
                     RGLEVWA"
                     /name="hypothetical protein"
     Typing          3911..3989
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.8899999999999995e-34"
     Typing          complement(3347..3835)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.747"
                     /coverage="94.0"
                     /evalue="7.82e-151"
     Typing          315..1810
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.998"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgctcacga tgtgggtaac agaagatgag caccgccgtc tgctggaacg ctgcgacggt
       61 aaacagctcg cggcctggat gcggcagacg tgcctggacg agaagcccgc ccgggccggc
      121 aaacttccct cgatctcgcc ggcgctgctt cgtcagcttg ccggcatggg gaacaacctc
      181 aaccagattg cccgccaggt taacgccggt ggcagtagcg gtctcgaccg cgtgcaggtc
      241 gtcgccgcgc tgatggccat cgatgccgga ctcgagcggc tgcggcatgc cgtactggaa
      301 aagggggctg acgatgatcg ttaagtttca tccccgggga cgcggaggcg gtgccggccc
      361 ggttgattat ctgctgggga aagaccgcca gcgcgacggt gccagcgttc ttcaggggaa
      421 gccggaggaa gtccgggagc ttatcgacgc ctcgccctac gccaaaaagt acacctccgg
      481 ggtgctgtcc tttgccgaac aggacttacc gcccgggcag cgtgaaacgc tgatggcgag
      541 cttcgagcgg gttctgatgc ccggactcga taaagaccag tacagcgtgc tgtgggttga
      601 gcaccgggac aaggggcggc tggagctgaa cttcctgatc ccgaacacgg agctgctgac
      661 cggcaaacgg ctccagccat actatgaccg cgccgaccgt cctcgcattg atgcctggca
      721 gaccatcgtg aacggcagtc tggggctgca cgacccgaac gcgccggaaa accggcgggc
      781 actggtcacg ccgtccgcgc tgccggaaac gaagcaggaa gccgctcagg cgattacgcg
      841 gggtttactg gccttagcct cgtccgggga gctgaaaacg cgtcaggacg tcactgaggc
      901 gctggaaagc gcaggttttg aggtggttcg caccacaaaa agcagcatca gcattgccga
      961 cccggacggg gggcgaaaca tccgactgaa gggagccatc tatgaacagt cttttaacgc
     1021 tggcgaagga cttagagcag aaatcgaaag tgcagcagca gactaccggc gagatgctga
     1081 aagccgcatt cagcgagcac gagaagtctg tcagagcgga actgaacgaa agcgagaaga
     1141 gaatcagcgc cgccatccac gaccacgacc ggatgctgtc ctcagccatg agccagcgta
     1201 cgaaagggat gctgcgcatg gtcagccaga cgtggctgac catcgtcctg gtctccgtgc
     1261 tgctgatagc ctcaagtgcg ggcattctat ggtggcaggg gcagcagata ctcgacaatt
     1321 acacgaccat ccgggagcag aagagcacgc aggccatgct gtccgagagg aacagcggcg
     1381 tgcagctcac gacctgcgga gaggaacgac gccgctgcgt gagggtgaac ccggacgcgg
     1441 gacggttcgg agaggattcg agctggatga tactggcggg gaaatagcac atgacggaac
     1501 tggaaaaaca gttgctgagc gcattagagc agctacagca ggactactcg aaaaggctgg
     1561 acgagtggga gagcgccttc gcggaatggc ggacgatgtg tggtcttatg caacgggaga
     1621 acgcagcgct gagcgagcgc gtcacggact tgagcatgca ggtgctgagt ttaagcgagc
     1681 agctgcgccg gttgtcgtaa ggctgaacga catcgaggca caccgggagc aggagcgcgc
     1741 agcgcagcac cagaaagcgc tggagctgga acgatcgcag cgtcagcagg agtatgacgg
     1801 tccgtcgctg tgataaccgg ataaaacggg gctttcattg aggatctgcg ccgaaacccg
     1861 gtgccattca cccggctgca tggctatgca gtcgggtgag atttatcagg cgcagaatgc
     1921 acggtttgcg gggtggttta ctgtcggttt tgcaggactg acgccgcatt cgcgctgaac
     1981 ccgtccggag aggtgcgggc aagggatgtt atggtaaaag catgatgcct ggcgggtacg
     2041 gaaaaattgt ggcgaccgag cctgcagacg caaaaatccg gcccatactt attcgtctcc
     2101 ttcatcagga atgcagtact atccagccac tgcaggtatt tggcccccag cgggggcttt
     2161 ttttgcctgt tgttggggca gataaagaaa agggccgcat tagcggccct tttcggagag
     2221 agcgatgcct caatgcggaa tttcagagac ttctttaacg tcggctttat tgatctgctg
     2281 tttgacacca tttgcgtctt tatagcccag caggcctgag tctgattctg taggcttgcc
     2341 gtcgctgaca attgtacgcc cgtcgttggt gtggatggca tagcttgtgc gcgtgcagcc
     2401 ggttagggct gaaagcgcaa ctgccgctgc gaacactgaa acaaaaatct ttttcaaagc
     2461 cagctccttt tatccgtatc agttgtctag ttcactcatg ttggtaattt tgtcacggtt
     2521 gatctgttcg gttcggcctg tatgggcatc tttgtaggat actaatcccg tatcactgtc
     2581 aactttgggt ttgccgtctg taacgatagt gcgatcatct gtggttttta tttcttggtt
     2641 tgaagaacat ccagcagcgg caaaggtcac caacgcgacc atcatggccg ccaacagcgt
     2701 ttttttattc ataataggtt ccctgcttaa aggtaaagtg gatatggtca ctaaccatct
     2761 aagtataggt cgttttttca tgcgaaaaga ggattgaatg gacaagaatt tcgattattg
     2821 ctgacagagc atggagaaaa agaggctgtt ttactgttcg gaaagaaatc ttttgttgcc
     2881 gtgccagata cgcagaacct ttatggtggg tgttattacc tttatttaga ggatgctcac
     2941 aagaacgagc ttttaattga gttaaacgaa agtgatttat accccggtgg cgcaatagaa
     3001 cttcaacaca acggcggtcg gaagattcat tgcgagatac taaaagtaag gtggagtcgt
     3061 taactcaagt aatgtttcct aagtatgttg ataggcaaac atagtggtag ttagtctctc
     3121 tgagtaaatg atcttcttgt gatcctgtcg ttacagtcgt aatctcttgc tctgaaaacg
     3181 aaaaaaccac ctggggaggt ggtttgatcg aaggttaagt cagttgggga actgcttaac
     3241 caggtaactg ggtatacgag accgcagaaa ccaaaactgt tctttcagtg tagcctctct
     3301 caggcccata cttcaagacc tcttaatttg tctctcgtat acctccagtt actaatggct
     3361 gctgccagta gcgatttttc gtgtctttcc gggttggact caagacgata gttaccggat
     3421 aaggcgcagc agtcggactg aacggggggt tcgtgcatac agtcaatcct ggagcgaact
     3481 gccttcgcgg aactgagtgt caggcgtgga atgagaaacc gcggccataa cagcggaatg
     3541 acaccggtaa accgaaaggc aggaatgcgg gggagcacga gggagccatc agggggaaac
     3601 gcctggtatc tttaagccgc atcgggtttc gccaccactg atttgagcgt cagattctgt
     3661 gatgcttgtc aggggggcgg agcctatgga aaaccggcag cggcgcatcc tttcttcccc
     3721 tcctgcattc acgtatttcc ccgccacgtc tttatccgtg agtccgtacc gctcgccgca
     3781 gccgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaatatcca ccgggacagg
     3841 catcagcctc ttacgcacct gagcctcatc tcacagtttt catgactgac tatttcatcc
     3901 cgattcccgt cgtgccacca tagtaagcca gtatacactc cgctagcgct acgtgactgg
     3961 ttcagggctg cgccccgaaa cccgctaaat gccactgacg cgcctgcggc ttgcccaaca
     4021 ccgcgccgac cgggaaaaga tttcccgccc gtcccggcgt tatcaggagg ccggattggc
     4081 agacaaacgc agcaaa
//
