LOCUS       NZ_CP103671             4938 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Klebsiella aerogenes strain 4417 plasmid pMB5632_7, complete
            sequence.
ACCESSION   NZ_CP103671
VERSION     NZ_CP103671.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28174061
            Assembly: GCF_024918355.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella aerogenes
  ORGANISM  Klebsiella aerogenes
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 470x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 16:48:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,733
            CDSs (total)                      :: 5,610
            Genes (coding)                    :: 5,500
            CDSs (with protein)               :: 5,500
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 39 of 110
            Pseudo Genes (incomplete)         :: 80 of 110
            Pseudo Genes (internal stop)      :: 12 of 110
            Pseudo Genes (multiple problems)  :: 17 of 110
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103671.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Klebsiella aerogenes"
                     /mol_type="genomic DNA"
                     /strain="4417"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:548"
                     /plasmid="pMB5632_7"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2018-05-11"
                     /collected_by="MDACC"
                     /genotype="ST208"
     gene            275..1561
                     /locus_tag="M5S98_RS28620"
                     /old_locus_tag="M5S98_28620"
     CDS             275..1561
                     /locus_tag="M5S98_RS28620"
                     /old_locus_tag="M5S98_28620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002437803.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_306170312.1"
                     /translation="MFSLNLQRSAISYDAICQNKPVIEFSPEGVINKASPLFLSTMGYR
                     ADEIIGHHHRIFCPPSLVSSPEYSQFWQRLARGESFSGKYLRLAKGNRSVWLEASYIPV
                     SDRRGRVIRVIKIAADISERVHSALEQEAVVNAINRSMAIITFNPEGIVLEANENFVNA
                     TGYKRDEIIGKHHRLFCAETLYKSDEYRHFWESLNQGEFFSGLFPRLNRQGDPLWFRAT
                     YNPVFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRESAQNGANVIENS
                     ILMIDRIAQGMGAVSTDISRLNNQSESIDDMVETIRKFAMQTRLIALNAAIEAARAGAS
                     GRSFAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLCGIENSLMNTREGVTLMRE
                     AGEVMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            2570..2893
                     /locus_tag="M5S98_RS28625"
                     /old_locus_tag="M5S98_28625"
     CDS             2570..2893
                     /locus_tag="M5S98_RS28625"
                     /old_locus_tag="M5S98_28625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007899679.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTAKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGVGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3102..>3536
                     /locus_tag="M5S98_RS28630"
                     /old_locus_tag="M5S98_28630"
                     /pseudo=""
     CDS             3102..>3536
                     /locus_tag="M5S98_RS28630"
                     /old_locus_tag="M5S98_28630"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3571..4062
                     /locus_tag="M5S98_RS28635"
                     /old_locus_tag="M5S98_28635"
     CDS             3571..4062
                     /locus_tag="M5S98_RS28635"
                     /old_locus_tag="M5S98_28635"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_016355729.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELNASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIATSGSILWWQGRQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESNDGRRFLILPPGMQAEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            4069..4284
                     /locus_tag="M5S98_RS28640"
                     /old_locus_tag="M5S98_28640"
     CDS             4069..4284
                     /locus_tag="M5S98_RS28640"
                     /old_locus_tag="M5S98_28640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          2283..2455
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="9.14e-86"
     Typing          complement(1812..2358)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.545"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          2571..2887
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.509"
                     /coverage="99.0"
                     /evalue="3.03e-59"
     Typing          2884..4385
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="83.692"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgctggttct tccaagattg atagacgcca gcttctctgc tggagccggc ggatttgccg
       61 taaatatgaa cattttctta ttgttcagcc cgctaaagat ggcgggtttt ttacgcgtga
      121 aatccggagc cggacatcat gccggcatgg ctgaagagcg ggctttttcc cgccctgtcg
      181 cagtaatata taacgctaca taaggagtag ccgataacca tggcagtact tacattatcc
      241 gggcaatctg attcatatgt tttcacttaa aaatatgttc tccctgaacc tccagcgatc
      301 agccatttcg tatgacgcaa tttgtcagaa taagcctgtc attgagttca gtccggaggg
      361 ggtgataaat aaagccagcc cgctttttct ctccactatg gggtacaggg ctgatgaaat
      421 aatcggtcac caccatcgta tattctgccc gccctcgctg gtaagttcac cggaatacag
      481 ccagttctgg cagcgccttg ccaggggaga aagctttagt ggcaagtacc tgcggctggc
      541 aaagggaaat cgttccgtat ggcttgaagc cagttatatc cccgtttctg acaggcgcgg
      601 ccgtgttatc agagtcatta aaattgctgc cgatatttct gagcgcgtgc attctgctct
      661 tgagcaggaa gcagtcgtaa atgccataaa ccgttctatg gccatcatca ccttcaatcc
      721 tgaagggatc gtgcttgaag caaatgaaaa tttcgtcaat gccactggct ataagcggga
      781 tgaaatcata ggtaagcatc atcgtctgtt ctgtgccgag acgctttaca aaagtgatga
      841 ataccgacat ttctgggaga gtctgaatca aggtgagttt ttttctggtc tgtttccacg
      901 ccttaaccgt cagggagacc ctctgtggtt ccgtgcaact tataaccctg tctttaacag
      961 tgatgggcag ctttataaaa ttgtgaaatt cgctacagat gttacggctg atgtcctgcg
     1021 aaaccagaga gagcaagagg ctgctgtgca tgcatgggat atggccgtgc agacccgaga
     1081 aagtgcgcag aacggtgcca atgttattga aaatagcatc cttatgattg acagaattgc
     1141 gcaggggatg ggggctgtct ccaccgatat ctcacggctc aacaatcagt ctgaaagtat
     1201 tgacgatatg gtggaaacca tccggaagtt tgccatgcag acaagactta ttgcactgaa
     1261 tgccgcgatt gaagcagcaa gggctggcgc atccggaaga agttttgcgg ttgttgccgc
     1321 agaagtgcgt aatcttgcgg cgagcgtcag tagcgcgact gaagaaattg agcaagtcgt
     1381 ggccagcaat agtcaactgg ccaaggatgt tctctgtggc attgaaaaca gcctgatgaa
     1441 cactcgggaa ggggttacac tcatgcgcga agcgggtgaa gttatgacca gcattcagag
     1501 gaatgctgca ggggttgaga ctgcggttaa ggatgtcgcc atttcagtta aagccggata
     1561 ggccatggtg catatcccca tatctgtcga acatcacaga tgatcttctt gagatccttt
     1621 ccgttgcgtc gtaatctctt gctctgaaaa cgaaaaaacc acccggcaag gtggcttttt
     1681 cgaaggttcg caagagttga cgctcttttg aaccgcggta actggcttgg aggagcacag
     1741 tcaccaaaac tgtcctttca gtgtagcctt atcgcacccg aaacttcaag actctcctcc
     1801 tctaaacctg ttaccagtgg ctgctgccag tggcgctttg tcgtgttttt ccggattgga
     1861 ctcaagttga tagttaccgg ataaggcgca gcggtcggac tgaacggggg gttcgtgcat
     1921 acagtccagc ttggagcgaa ctgccttccc ggaactgagt gtcaggcgtg gaatgagaca
     1981 aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca
     2041 cgagggagcc accaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     2101 actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg
     2161 gcttcgccgc ggccttatcg cttccctgtt aagcttcttt ctggcatctc ccaggaaatt
     2221 tccgccccgc ctgtaagccg gtaccgctcg ccgcagccga acgaccgagc gcagcgagtc
     2281 agtgagcgag gaagcggaat atatcctgta tcgcatattc tgctgacgct ccgctgctgc
     2341 atttttctcc tgccacatga agcacttcac tgatattcgc atccgtgcca acatagtaag
     2401 ccagtataca ctccgctagc gctacgtgac tggttcaggg ctgcgccccg aaacccgcta
     2461 acagccactg acgcgcctgc ggcttgtcca accccgcgcc gaccgggaaa ggttttcccg
     2521 cccgtcccgg ggttatcagg agactgtttt gtcagagaaa cggaacaaga tgctcaccat
     2581 gtgggtgacg gaggatgagc acaggcggct acttgagcgc tgcgacggca ggcagctggc
     2641 ggcgtggatg cggcagacct gcctggacga aaagccggct cgtaccgcaa aacttccttc
     2701 gatctcgccg gcgctgctgc gccagcttgc cggtatgggc aacaacctga accagatagc
     2761 ccgccgtgtt aacgccggcg tcggtaccgg tcatgaccgg gtgcagattg tggcggcgct
     2821 gatggccatc gatgccgggc ttgagcggct gcggcacgcc gttcttgaga aaggaacgga
     2881 cgatgatcgt taaatttcat ccacgcggtc gcggcggtgg cgccgggccg gtggactatc
     2941 tgctgggtaa agaccggcag cgtgaaggcg ccagcgtcct gcaggggaag ccggaagaag
     3001 tccgggagct gatcgacgcg tcgccttacg tgaagaaata cacctccggc gtcctgtcgt
     3061 ttgcggaggc cgatctgcct cccggccagc gggaaaaact gatggccagc ttcgagcggg
     3121 ttctgatgcc cggacttgat aaagatcagt acagcattct gtgggtggag cacgaggaca
     3181 aggggcgtct ggagctgaac tttctgatcc ccaataccga gctgctgacc ggcaggcggc
     3241 tccagccgta ctacgaccgc gccgaccggc cgcgtatcga tgcctggcag accatcgtga
     3301 acggcagact ggggctgcac gatccgaacg cgccggaaaa ccgccgggcg ctggtcacgc
     3361 cggccggact gccgaaaacg aagcaggaag ccgcagaagc catcacccgt ggactgctga
     3421 gcttagcctc gtccggggag ctgaaaagcc gtcaggacgt cactgaggct cttgaaggtg
     3481 caggttttga ggtggtgcgt acaacgaaaa gcagtatcag cattgccgac cctgacgggg
     3541 ggcgaaacat ccgactgaag ggagccatct atgagcagtc ttttaacgct ggcgaaggac
     3601 ttagagcagc aatcgaaagc gcagcagaag agtaccggcg agatgctgaa agccgcattc
     3661 agcgagcacg agcagtctgt cagagcggaa ctgaacgcaa gcgcgaggag aatcagcgac
     3721 gccatcagcg cccacgagca gagtatgagc gaggcgatgg agaaaaaccg gcggagcgtg
     3781 ttgctgacgg ccgggaggac atggctcacc atcctgatgg tgtcagcgct gctgatcgcc
     3841 acgagcggga gcattctgtg gtggcaggga cggcagataa ccgacaatta cacacatctt
     3901 cgtcagcagg aagataccct ggcgaaaatg accgcccgga cgtggggagt acgctatcag
     3961 gagagcaacg acggccggag gttccttatc ctgccgccgg ggatgcaagc cgaagccatc
     4021 ccctacgacg ggacgacctg gatccgcctg aaacaggagt agcggaaaat gacagagctg
     4081 gaaaagcagt tgctgagcgc attagagcag ctacagcagg actactcgca aaggctggac
     4141 gaatgggaga gcgccttcgc ggaatggcgg agcatgtgtg gacttatgca acgggagaac
     4201 gcggtgctga acgagcgcgt gtcggacttg agcacgcagg tgctgagttt aagcgagcag
     4261 ctgcgccgct tgtccgggaa ctgaatgcca tcgaggagaa ggctgcactg gagtatcagc
     4321 gtcagaacga gctggagctg acgctccgac agaagcagaa gagtttcgat ggtccgaaga
     4381 tgaggtgaca gcccgcacga agaagggctc atcgcgttcc tgctaaatag gctgactacc
     4441 ggaatggtag tgacttatcc cggaggaagt gatgaaaatt ctcaacgtta ttgaagccct
     4501 gctgaactgt ttttacctgg tgatggagct gtgtgagttg ccggagaggc atttgccggt
     4561 ttttaaggcg ttcgcgctga ttttagtgtg actttacgct gcatgcatct ggctgcatga
     4621 ctatgcatca gtgtaatatc cagcggctgc gtttttggtg ttttttcgtc atgtctgtcg
     4681 ccactttctc gcgtctggca ccggaaacgc agccaacccc gtttctgcgg tgcgggcaag
     4741 gggtgttatg ttaaaagccg aggtctggcg gatacggaaa aaattatgcc ggccgggctt
     4801 atgcccgcgg caatccggcc gatacttaaa ttatccttca caaaggatgt gctgcgatcc
     4861 aggcagttga gtgttttccg gctcccggat gtgagccatt tttttgcctt ctgatgcctg
     4921 ttaatgcatg cggagctg
//
