LOCUS       NZ_CP103670             5841 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Klebsiella aerogenes strain 4417 plasmid pMB5632_6, complete
            sequence.
ACCESSION   NZ_CP103670
VERSION     NZ_CP103670.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28174061
            Assembly: GCF_024918355.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella aerogenes
  ORGANISM  Klebsiella aerogenes
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 470x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 16:48:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,733
            CDSs (total)                      :: 5,610
            Genes (coding)                    :: 5,500
            CDSs (with protein)               :: 5,500
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 39 of 110
            Pseudo Genes (incomplete)         :: 80 of 110
            Pseudo Genes (internal stop)      :: 12 of 110
            Pseudo Genes (multiple problems)  :: 17 of 110
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103670.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Klebsiella aerogenes"
                     /mol_type="genomic DNA"
                     /strain="4417"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:548"
                     /plasmid="pMB5632_6"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2018-05-11"
                     /collected_by="MDACC"
                     /genotype="ST208"
     gene            join(5168..5841,1..151)
                     /locus_tag="M5S98_RS28585"
                     /old_locus_tag="M5S98_28585"
     CDS             join(5168..5841,1..151)
                     /locus_tag="M5S98_RS28585"
                     /old_locus_tag="M5S98_28585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_259418661.1"
                     /translation="MYNDNRQNFISQMPYKPYCSNDLTNGLTIRKREKALEMLYIQANQ
                     PTIQTCLLFDIDKENSFYTFEEAGLPVPQFITKSPDSGRCHYGYILRTGVCKTQQARLK
                     PLKYAAAVENGIAEALGSDRAYVGLVTKNPLNSHWSPYWSGADLYELDFLADCVDLTAP
                     KQAIKTENYGLGRNVNLFEDLRFYAYKHVLSFKKSSNFDRFLIEMESQAINLNAVCNVG
                     NLLPFSEIKATVKSICRWTWKNFDSATFSAIQSARAKKPRKTADLIKYLEDL"
                     /name="replication initiation protein"
     gene            148..411
                     /locus_tag="M5S98_RS28590"
                     /old_locus_tag="M5S98_28590"
     CDS             148..411
                     /locus_tag="M5S98_RS28590"
                     /old_locus_tag="M5S98_28590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324694.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_259418662.1"
                     /translation="MIVKKRIKRPMTQKEMAEKFNISISTVKNYIALPREEYEDEAKRR
                     RFKAYELKQNGLKLAEIAEVLGCSYHAAAGLIKRYKQQDLVA"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(512..1882)
                     /locus_tag="M5S98_RS28595"
                     /old_locus_tag="M5S98_28595"
     CDS             complement(512..1882)
                     /locus_tag="M5S98_RS28595"
                     /old_locus_tag="M5S98_28595"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_259418663.1"
                     /translation="MIHKIIGGKTKLNQRNIKNSISYLLREKKPAEQDLIRVLSGSKEQ
                     MLNFNSLIASKDLKTPYVAGVLSFEEENIPEAEKFKIMNDFEAMAFSGVPEEFRPPVLW
                     VEHRDKGRLELNYLTFNSLTTGRSFPVYYDKKDRRLFNDFTEVINYEGGYSSPFDDFTN
                     TQKIRMAEPPGKTLPAEKKALVEGLNNRLCSLILERKITNKADLITYLQEKEGFKINRI
                     AKNTVSIITDLDDTPIRLKGDIYQDGRDFGDYFKEKQAKPERTQAEISEKLSNHKPSYQ
                     KGLECRTERNRRVYIDPIIRRQQKEEELNKLTIKNEIKPEADTVAVTIPEVNNYDNKRE
                     LTESPATTIFSKIDRIGEQQSKIDYEQQSIRERLDDVGNKINITDGGIGSIGRRFGFFG
                     DYFDRFSIFIEEKLRTVVEFITEAERKNKEKRQQKQKQEVKPKETEVTRRPRFGKKY"
                     /name="hypothetical protein"
     gene            complement(1879..2280)
                     /gene="mobC"
                     /locus_tag="M5S98_RS28600"
                     /old_locus_tag="M5S98_28600"
     CDS             complement(1879..2280)
                     /gene="mobC"
                     /locus_tag="M5S98_RS28600"
                     /old_locus_tag="M5S98_28600"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_142900245.1"
                     /translation="MREKRKIKRIEPELSPVSKNYKAKYKRKNMVSLCLNDDEYLKIEK
                     ITSALNVPRATASREILISNADDILSKIKRNDNKVLVLELNKIGNNLNQITKKINSNLE
                     LCLSGEGENFALAVDRLNETFNKILEAIK"
                     /name="mobC"
     gene            complement(2569..3096)
                     /locus_tag="M5S98_RS28605"
                     /old_locus_tag="M5S98_28605"
     CDS             complement(2569..3096)
                     /locus_tag="M5S98_RS28605"
                     /old_locus_tag="M5S98_28605"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_259418664.1"
                     /translation="MTLNNNDIEDLNPDHMFDHLKNIFNEREEEEKEEKLFTEKELLKL
                     FEDKIWEDLQKIKMECNSKNLQAYCSAALKNYKRNLVCLHIDKIKEIEGLKLTTRAADM
                     FFISYLGRSVGEKFLCENFGREFKSGDKIILTDKYKGELNLIILKYKDEFKGNLFFIIS
                     LYRKFKNYIIYA"
                     /name="hypothetical protein"
     gene            complement(3080..3331)
                     /locus_tag="M5S98_RS28610"
                     /old_locus_tag="M5S98_28610"
     CDS             complement(3080..3331)
                     /locus_tag="M5S98_RS28610"
                     /old_locus_tag="M5S98_28610"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_259418659.1"
                     /translation="MYIKTKTNIIFDENDPICRYVVSMAEGKTFAFIRCYQDHPENIKF
                     YWGLVDETTEDSRKETITHIINYGSKWDQVGRLYDFEQ"
                     /name="hypothetical protein"
     gene            complement(3657..4829)
                     /locus_tag="M5S98_RS28615"
                     /old_locus_tag="M5S98_28615"
     CDS             complement(3657..4829)
                     /locus_tag="M5S98_RS28615"
                     /old_locus_tag="M5S98_28615"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_259418660.1"
                     /translation="MKKIKCNGFTLIEILLVIGVIMIMSIIKIRDINEETENLQSKMLA
                     SQIKTVADATNAFLVLKYSELSTLSVSGCNSSNQTCSITLQQLNDEDLLPPNFATTTIL
                     GQPYQIQLKRTGNAPNYMISGIVLTKGTKANNATPSRVFMGKVLKEIGREGGLNKDKGN
                     ITGTAGGWSADSNLFPILSEKVNYIGSAVGSLSGAYYVYLRRDGTLPMTGDLNMDGHNI
                     YNVKNLTATGALTTTGDISGKDINATGNISVSGAANVNTLESTGRATVGEFIQLNGQAT
                     VGATCSPNGLQGRTSAGEHVSCINGVWSKLSLTGSLYSVRFDATTAGSGDVFKTLNLGQ
                     HLFCVFSGDQTTIGMVKHSRVYQKGADWILEMRVIKWESSNSWAYATCYD"
                     /name="hypothetical protein"
ORIGIN
        1 tccatttagt gaaataaaag caactgttaa aagtatatgt aggtggactt ggaaaaattt
       61 tgatagtgct actttttcag ctattcaatc agcccgcgca aaaaaaccaa gaaaaaccgc
      121 tgatttaatt aaatatttgg aggatttatg attgttaaaa aaagaattaa aagacctatg
      181 acacaaaaag agatggcaga aaaattcaac atctcaattt caacagtaaa aaattatatt
      241 gctttacctc gtgaagaata tgaagatgaa gcaaaacgcc gaagatttaa agcctacgaa
      301 ttaaaacaga atggcttaaa actcgctgaa atagcggagg ttttgggttg ttcttatcac
      361 gcagcggcgg gactaattaa gcgatataaa cagcaagatt tagtcgctta attagctatg
      421 ctggggctaa ataatcgctt atatagcccc ttccttttgg gttgtttttt cataaaaact
      481 cacaagaaaa gccatcgtgt tttttatttg atcaatattt ttttccaaat cttggtcgtc
      541 ttgtaacctc tgtctctttt ggcttaactt cttgtttttg tttttgttgc cttttttctt
      601 tgttctttct ttcggcttcg gtaataaatt cgacaactgt tcgcaacttt tcttcaatga
      661 agatactaaa tcgatcaaag tagtctccaa aaaatccaaa tcttcttcca atactgccaa
      721 ttcctccgtc tgtgatattg attttgtttc caacgtcgtc aagtctttca cgaattgatt
      781 gttgttcata atcaatcttt gattgttgtt ctccaattct gtcaatcttg ctaaaaattg
      841 ttgttgctgg gctttctgta agttctcttt tattgtcata attatttacc tctggaatag
      901 taacggccac agtatcggcc tctggtttga tttcattttt tatagttaat ttgtttaatt
      961 cttcttcttt ttgttgcctg cgaataatcg gatcaatgta gacccgtcta tttcgctctg
     1021 tacggcattc taagcccttc tgatagctcg gcttatgatt gcttaacttc tcagagatct
     1081 cggcttgtgt tcgctctggt ttagcctgtt tttctttgaa gtaatcgcca aaatcacgac
     1141 catcttgata aatgtcgccc ttaagtctga tcggtgtgtc atcaagatcg gttatgattg
     1201 aaaccgtgtt tttagctatc ctattgattt taaagccttc tttttcttgc aggtaggtta
     1261 taaggtcggc tttattggtg atttttcgct ctaatataag actacataag cgattattta
     1321 gcccctctac aagtgccttt ttttctgctg gcagggtttt tccgggcggt tctgccattc
     1381 tgatcttttg tgtgtttgtg aagtcgtcga acgggctgct ataaccacct tcataattta
     1441 tcacctctgt gaaatcatta aataggcggc gatctttttt gtcatagtaa acaggaaagc
     1501 tcctgcctgt ggtgaggctg ttaaatgtta aataattaag ctccaatctt cccttgtctc
     1561 tgtgttcaac ccaaagcaca ggggggcgaa attcttctgg aactccagaa aatgccattg
     1621 cctcaaaatc attcattatt ttgaattttt cggcctctgg tatgttttct tcttcaaatg
     1681 acaaaacccc cgcaacatat ggagttttta aatcttttga ggctatcaaa ctattaaaat
     1741 ttagcatctg ctcttttgaa cctgataaaa ccctaatcag atcttgctct gctggttttt
     1801 tttctctcaa caaataagat atagaatttt taatatttct ttgatttagt tttgttttgc
     1861 ctccgattat tttatggatc attttatagc ctccaaaatt ttgttaaatg tttcatttaa
     1921 acgatcaaca gcaagagcga aattttcccc ctcgcctgat aaacacaatt caagattaga
     1981 atttattttt tttgtgattt ggtttaaatt gtttccgatt ttgtttaatt ccaacactaa
     2041 tactttgttg tcgtttcttt tgattttaga caaaatatca tcagcatttg atattaaaat
     2101 ttctctcgat gctgtggcac gaggaacatt taaagcagat gttatttttt caatttttaa
     2161 atattcatca tcatttaaac acaaactgac catatttttt cttttgtatt ttgctttata
     2221 gttttttgag acaggcgaaa gctctggctc tattcttttg atttttcttt tttctctcac
     2281 aaacacactc cgaattatct tttttctttt gattttgacc tggcttttgg tggggattga
     2341 aggggaggcg aagccacccc tcaccagcga gcaatccgaa ggattgattg tgctggctct
     2401 ctacatttaa aacctcataa acttaataat atataaaaaa aataagtcaa gcctgagcga
     2461 ttaattagca agcaacaacg gcgaaaaaaa acgacctgca cgaaagaaaa aagccttaca
     2521 aatcaataac ttataatatt atttttttta atgctaatta agcgattatt aagcataaat
     2581 aatataattt ttaaacttac gatataggga aataataaaa aataaatttc ccttaaattc
     2641 gtctttatat tttaaaataa ttaaatttag ctcgccttta tatttgtctg ttaatataat
     2701 tttatctccg cttttaaatt ctcgaccaaa gttttcacat aaaaactttt ctccgacaga
     2761 tcgtccaaga taactaataa aaaacatatc tgcggctctg gttgttagtt ttaagccctc
     2821 tatttctttt attttatcaa tgtgtaagca aactaaattt cttttataat ttttcaatgc
     2881 tgcggaacag taggcttgta aattctttga attacattcc atttttattt tttgtaaatc
     2941 ttcccaaatt ttgtcttcaa acaattttaa caattctttc tctgtgaata gtttttcttc
     3001 tttttcttct tcttctcttt cattaaatat gttttttaaa tgatcaaaca tatgatcagg
     3061 gtttaaatct tctatatcat tattgttcaa agtcatataa cctcccaacc tgatcccatt
     3121 tagaaccata attaataata tgtgtgattg tttcttttct gctgtcctct gtcgtttcgt
     3181 ccaccaaacc ccaataaaat tttatatttt ccgggtgatc ctgataacaa cggataaaag
     3241 cgaaagtttt accctccgcc atagagacaa cataacggca tatagggtcg ttttcgtcaa
     3301 aaatgatatt tgtttttgtt ttaatataca taaaagctcc ttagttaatt aatcgcttat
     3361 atagtctatt taatacatta aaaataaaag tcaaatttat tttatacttt aaagtcaaaa
     3421 gaaaaaacgg aaggggtttt attttccgag atgcggaacg gatcagggtg ttaatggcaa
     3481 tcccaaacag gaaaaatttt aatttttgct gtttgggatt aataaggtgt taaagttatt
     3541 taagcgatta attagctatt ggatgcagat tttttttgga atggcattaa aaagcaccgc
     3601 catcaggcgg tgctacgtga aatctggcag tttttgtggg gtaagttagg taaattttaa
     3661 tcataacaag ttgcatatgc ccaagaatta ctcgattccc atttaatcac tctcatctcc
     3721 aatatccaat cagcaccctt ctgatataca cgagaatgtt ttaccatacc tattgttgtt
     3781 tgatcaccag aaaaaacaca aaataaatgc tgtccaagat ttaaagtttt aaaaacatcg
     3841 ccactaccag cggtagtagc atcaaaacga acactataaa gagagcctgt taaactaagt
     3901 ttactccaca cgccattaat acaagaaaca tgttcacccg ccgatgttct tccctgtaat
     3961 ccattaggcg agcacgtagc cccaaccgta gcctgtccat taagttgtat aaattcaccc
     4021 acagtggctc gtccagtgga ctctaatgta tttacattag ctgcgcctga aacagaaata
     4081 ttaccagttg cattaatatc tttacctgaa atatctcctg tcgttgtcaa cgcccccgta
     4141 gcggttaaat ttttcacatt atagatatta tggccgtcca tattcagatc gcccgtcata
     4201 ggcaatgtgc catcacgcct taaatagacg tagtaagccc ctgaaaggct tccaacggct
     4261 gaaccaatat aatttacttt ctcagatagg attggaaata aattgctgtc agccgaccag
     4321 cccccggcgg ttcccgtgat atttccttta tctttgttca atcctccctc tcgtccgatt
     4381 tcttttaaca ctttacccat aaaaacacgc gatggcgtag cattattggc ttttgttcct
     4441 tttgttaaaa caatccctga aatcatataa ttaggggcat tacctgttct ttttaactga
     4501 atctgataag gttgtccaag aatagttgta gtagcaaaat taggaggcaa caaatcctca
     4561 tcgttcagtt gttgtaatgt gatggaacac gtttgattag agctattaca accagatacg
     4621 gaaagagtgg aaagctcact gtattttaat acaagaaaag cattagttgc atctgcgaca
     4681 gttttaattt gactcgcaag cattttagat tgtagatttt cggtttcttc gttgatatct
     4741 ctaattttaa taatcgacat aatcataata acaccaataa ccaacaatat ttcgattaaa
     4801 gtaaacccat tgcattttat ttttttcatt ggtacaccac tcatattatt ttaatagtga
     4861 tgtttaaata aaatggaatt ttgttttgtc aactataaaa aaggaatgtc atctggatga
     4921 tgatcatcat agtcctcccc taccctacca tctcactcca aaactccacc atcaaggctg
     4981 gttggtagtc ataaaggatt gctcgcctgg cagggccaaa accccttaaa aggggctgtt
     5041 atctgatatg gctaatggct acttgacaat ttttttttat gtattacaac ataagaaaac
     5101 aaaaaacccc ctatgttgtt gatagtttgg cgactgttca acataggggg tatagaaagg
     5161 actgcttatg tataacgata atcgacaaaa ttttataagt caaatgccct acaagcccta
     5221 ttgtagcaac gatctgacca atggcctgac tatcaggaag agggaaaaag ccctcgaaat
     5281 gctctacata caagcaaacc agccaacaat acaaacctgc ttgctgtttg acatagataa
     5341 agagaatagc ttttacacat tcgaggaggc tggcttgcct gtgcctcagt tcataaccaa
     5401 aagcccagac tctggaagat gtcactatgg ctacattttg cgaactggtg tgtgtaagac
     5461 acaacaagca agattaaagc cgttgaagta tgccgcagcc gttgaaaatg gcatagctga
     5521 ggctttgggt tcggatcggg cttatgtcgg tttagtgaca aaaaacccgc ttaactcgca
     5581 ctggtcgccc tattggtcgg gtgctgattt gtacgaattg gattttttag ctgattgtgt
     5641 agacctgaca gcaccaaaac aagcaataaa aacggaaaat tacgggcttg gtcgcaatgt
     5701 taacttattt gaagatctgc gattttatgc ctataaacac gttttaagct ttaaaaaatc
     5761 gtcaaacttt gaccgctttt taattgaaat ggaaagccaa gctataaatc ttaatgcggt
     5821 atgtaatgtt ggcaaccttc t
//
