LOCUS       NZ_CP103669             6282 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Klebsiella aerogenes strain 4417 plasmid pMB5632_5, complete
            sequence.
ACCESSION   NZ_CP103669
VERSION     NZ_CP103669.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28174061
            Assembly: GCF_024918355.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella aerogenes
  ORGANISM  Klebsiella aerogenes
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 6282)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 470x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 16:48:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,733
            CDSs (total)                      :: 5,610
            Genes (coding)                    :: 5,500
            CDSs (with protein)               :: 5,500
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 39 of 110
            Pseudo Genes (incomplete)         :: 80 of 110
            Pseudo Genes (internal stop)      :: 12 of 110
            Pseudo Genes (multiple problems)  :: 17 of 110
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103669.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Klebsiella aerogenes"
                     /mol_type="genomic DNA"
                     /strain="4417"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:548"
                     /plasmid="pMB5632_5"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2018-05-11"
                     /collected_by="MDACC"
                     /genotype="ST208"
     gene            join(5985..6282,1..26)
                     /locus_tag="M5S98_RS28540"
                     /old_locus_tag="M5S98_28540"
     CDS             join(5985..6282,1..26)
                     /locus_tag="M5S98_RS28540"
                     /old_locus_tag="M5S98_28540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_259418656.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSL
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGRDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            235..>669
                     /locus_tag="M5S98_RS28545"
                     /old_locus_tag="M5S98_28545"
                     /pseudo=""
     CDS             235..>669
                     /locus_tag="M5S98_RS28545"
                     /old_locus_tag="M5S98_28545"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            704..1189
                     /locus_tag="M5S98_RS28550"
                     /old_locus_tag="M5S98_28550"
     CDS             704..1189
                     /locus_tag="M5S98_RS28550"
                     /old_locus_tag="M5S98_28550"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_259418657.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLTASGASILWWQGQQIL
                     DNYTTIREQKRTQAMLSERNSSVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            1193..1408
                     /locus_tag="M5S98_RS28555"
                     /old_locus_tag="M5S98_28555"
     CDS             1193..1408
                     /locus_tag="M5S98_RS28555"
                     /old_locus_tag="M5S98_28555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_058671277.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1537..2055
                     /locus_tag="M5S98_RS28560"
                     /old_locus_tag="M5S98_28560"
     CDS             1537..2055
                     /locus_tag="M5S98_RS28560"
                     /old_locus_tag="M5S98_28560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_011017199.1"
                     /translation="MKCFWACSVSVVLVMSFTVASAENITHTKPLARSDLYLTCKGYQV
                     KGASVINVEKPSYLEGTGEQVEFAIISLGDASDGLKVFFDSWGFSPFGLIESHVMHEHW
                     SLVHVGGRVSVKGEKIDYSFVDNQPQEGGSYKPKTGVFLYGCINSDKRPSWVEKIYTRT
                     GVFRGGIED"
                     /name="hypothetical protein"
     gene            2504..3040
                     /locus_tag="M5S98_RS28565"
                     /old_locus_tag="M5S98_28565"
     CDS             2504..3040
                     /locus_tag="M5S98_RS28565"
                     /old_locus_tag="M5S98_28565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048237141.1"
                     /translation="MFDTDCITVLTARGITEILNSGGSQAWRLDATHVAKLPYLVCVQN
                     SKKDWGSQEAKHHHAFMIGKISGVSRAPENPKRWIINIDSYAEIDIPGQWDGNRNPVSY
                     RKLEDMGIDISKVDFQPLPKEDSLVLNTIKGGDEYDEYEEDSNRVRPINLKEAKAGLAL
                     YFGVNEDDIQITIKG"
                     /name="hypothetical protein"
     gene            3045..3635
                     /locus_tag="M5S98_RS28570"
                     /old_locus_tag="M5S98_28570"
     CDS             3045..3635
                     /locus_tag="M5S98_RS28570"
                     /old_locus_tag="M5S98_28570"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_259418658.1"
                     /translation="MPSLSVERWLALATAGDSQARFHLGKIFSCGLGVHQDYKQAIYWF
                     QLAAEQGHDDAQFSLGWAYHLGLGGYKDAQKASEWYQKAAEQGHAEAQFQLGRLYTFSE
                     LRNDALAVEWFLCAAKQDHSSAQFKLGWMYHLGMGVEQSDRQAFHWFILAAEQDHAGAQ
                     ASLGEMYKHGWGVEQDDVAAQDWFYRARTMHYP"
                     /name="tetratricopeptide repeat protein"
     gene            3827..4201
                     /locus_tag="M5S98_RS28575"
                     /old_locus_tag="M5S98_28575"
     CDS             3827..4201
                     /locus_tag="M5S98_RS28575"
                     /old_locus_tag="M5S98_28575"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_259418654.1"
                     /translation="MNDISDVRLKKTTVDSLKEYVNDFGMTVDDVVLNLIEENNSLKSL
                     LGQDFSLPRGDKDTYLFKEYVIHKLPKGKILVTKNGLKVSNMKKVLIEAGSALGMDDKE
                     LNPDFTTYEIGRFLFKHLHG"
                     /name="hypothetical protein"
     gene            4337..4858
                     /locus_tag="M5S98_RS28580"
                     /old_locus_tag="M5S98_28580"
     CDS             4337..4858
                     /locus_tag="M5S98_RS28580"
                     /old_locus_tag="M5S98_28580"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_259418655.1"
                     /translation="MFGFVKKAVNAVSDTASSALSSASKAATSASASISDKASSTYSSI
                     SDSAEKITGSVSGAAQSAWLMAESVITDGLLDLTSDFLKDDEKIREVSLIFWQRIPREV
                     RFFLSEDKFHERTKSLRDNALLKLDESKQKRILIPEATNAEDAIPVLISNEPASVTTLP
                     DGLQAKNSQG"
                     /name="hypothetical protein"
     Typing          5699..5881
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.127"
                     /coverage="86.0"
                     /evalue="9.819999999999999e-42"
     Typing          complement(4978..5499)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.218"
                     /coverage="99.0"
                     /evalue="7.8e-166"
     Typing          17..1500
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.313"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          5986..6281
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.99"
                     /coverage="93.0"
                     /evalue="4.78e-58"
ORIGIN
        1 aaaagggtgc agacgatgat cgttaagttt cacccgcgag ggcgcggcgg tggcgccggt
       61 ccggtggatt atctgctggg gaaagaccga cagcgcgacg gcgccatcgt tctgcagggg
      121 aagccggaag aagtcaggga gcttatcgac gcctcgccct acgcaaaaaa gtacacatcc
      181 ggcgtcctgt cctttgccga aaaggattta ccgcccgggc agcgcgaaaa gctgatggcg
      241 agctttgaac gggttctgat gcccggactc gataaagacc agtacagcgt gctgtgggtt
      301 gagcaccagg ataaggggcg gctggagctg aacttcctga tcccgaacac ggagctgctg
      361 accggcaagc gtctccagcc gtattacgac cgcgccgacc gtccgcgcat tgatgcctgg
      421 cagaccatcg tgaacggcag gctggggctg catgacccga acgcgcctga aaaccggcgg
      481 gcgctggtca cgccgtccgc gctgccggaa acgaagcagg aagccgctca ggcaattaca
      541 cggggtttac tggccttagc ctcgtccggg gagctgaaaa cccgccagga cgtcactgag
      601 gcgctggaaa gcgcaggttt tgaggtggta cgcaccacaa aaagcagcat cagcattgcc
      661 gacccggacg gggggcgaaa catccgactg aagggagcca tctatgaaca gtcttttaac
      721 gctggcaaag gacttagagc agaaatcgaa agcgcagcag cagagtaccg gcgagatgct
      781 gaaagccgca ttcagcgagc acgagaagtc tgtcagagcg gaactgagcg aaagcgagaa
      841 gagaatcagc gccgccatcc tcgaccacga caggaagctg tcctcagcca tgagccagcg
      901 cacgaaaggc atggtgcgta tggtcagcca gacgtggctg accatcgtcc tggtgtctgc
      961 actgctgaca gcatcgggcg caagcattct gtggtggcag gggcagcaga tactcgacaa
     1021 ttacacgacc atccgggagc agaagcgcac gcaggccatg ctgtccgaga ggaacagcag
     1081 cgtgcagctc tcgacctgcg gagagcagag acgccgctgc gtgagggtga acccggaagc
     1141 gggacggttc ggagaggatt cgagctggat gatactggcg gggaaatagc acatgacgga
     1201 gctggaaaaa cagttgctga gcgcattaga gcagctacag caggactact cgaaaaggct
     1261 ggacgagtgg gagaacgcct tcgcggaatg gcggacgatg tctggtctta tgcaacggga
     1321 gaacgcggcg ctgagcgagc gcgtcacggg cttgagcagg caggtgcaga gtttgagcga
     1381 tcagctgcgc cggttgtcga aaggctgaac tcgattgaag cacacaggca gcaggagcgg
     1441 gcggcgcagc atcagaaaac gctggagctg gaacgatcgc agcggcagaa aggttttgag
     1501 cttggaatgt agttaatcaa tgaaaaggga tgcgttatga agtgtttctg ggcttgctca
     1561 gtctcagtag ttttggttat gtcatttaca gtcgcttctg cggagaacat tacacacaca
     1621 aaaccgttag ccaggagcga cttatatctt acctgtaaag gttatcaggt gaagggggcg
     1681 tctgtaatta acgtcgagaa accttcttac cttgaaggta caggtgagca ggttgagttt
     1741 gcaatcattt ctctcggtga tgcatctgat ggactcaagg ttttttttga ttcatggggt
     1801 ttttctcctt ttggattgat tgaaagtcat gtaatgcatg agcactggtc tttagttcat
     1861 gttggggggc gtgtttcagt caagggtgaa aagattgatt attcatttgt agataaccag
     1921 ccacaggagg gtgggagcta taaacccaaa acaggtgtgt tcctgtatgg ctgcattaac
     1981 tctgataaaa ggccatcttg ggtcgaaaaa atctacacaa gaacaggcgt gtttcgaggg
     2041 ggcatagaag actaaaatac tgcgtacagc atgaaaattc attgaacaga gtgttagttc
     2101 attttcgtgc atggctatgc atacacgcaa aacttatcag cgcgattccg ggcggttttc
     2161 ggtgagaaga cgggctcttt tgtggctcag gacggaaaaa cgccctgagc gcgtctgagc
     2221 ggtgcgccca agaactctta tgttaaatgc ggcggtgtgg cgggttcata cttcacacat
     2281 catgttgtca ctcagactga gtccagagag aaaaagccct ccggccgaag ttgagtggtg
     2341 ttttactggt tttttttgca tctccatggg taatcgtagt agtacttaag ctagttattt
     2401 gcatgtatct tgtagtttta tgttgagctc ttccgttttt gatgctaaaa agattgtcat
     2461 tacgcggtga cttgcctttt ttaaaacaaa aatggagata tctatgtttg atactgactg
     2521 tattactgtt cttacagcta ggggtattac tgagatttta aatagtggag gaagccaggc
     2581 ttggagactt gatgctactc acgtagctaa gcttccgtac ctggtttgcg tccaaaattc
     2641 gaaaaaagac tggggtagtc aggaggctaa gcatcatcat gcatttatga tcgggaaaat
     2701 atccggagta agtcgagccc ctgaaaaccc taagcgctgg atcataaata ttgattccta
     2761 tgctgaaatt gatatccctg gtcagtggga cggcaatcgt aatccagtct cctatagaaa
     2821 acttgaagac atgggaattg atatctccaa agtggatttc caacccctac ctaaagagga
     2881 ttccttagtc ttgaatacga taaaaggcgg ggatgaatat gatgagtatg aggaggatag
     2941 caacagagtt aggccgataa atctaaaaga ggctaaagca gggttggctt tatattttgg
     3001 tgtgaatgag gatgatattc agatcacaat taagggatga ccttatgccg tcactctctg
     3061 ttgagcgctg gttagcacta gctactgctg gagatagcca ggctagattt catctgggta
     3121 agatattctc atgcggtctt ggtgtgcatc aggattataa gcaggctata tactggtttc
     3181 agcttgcagc tgagcaaggc catgatgatg cccagtttag tcttggttgg gcatatcatc
     3241 tggggttagg tggatataaa gatgctcaaa aagcttcgga atggtatcag aaagctgctg
     3301 agcaaggtca tgcagaggct cagtttcagc ttggaaggct gtatactttc tctgagttaa
     3361 gaaatgatgc gttagcagtc gaatggtttc tctgtgctgc gaagcaagac cacagtagtg
     3421 cccaatttaa acttggctgg atgtaccatc tcggtatggg cgttgagcaa agtgataggc
     3481 aggctttcca ttggtttatt ctggccgctg agcaagatca tgcaggagct caggcatctc
     3541 ttggtgagat gtataaacat ggatgggggg tcgagcaaga tgatgttgct gcgcaggatt
     3601 ggttttatcg tgccagaacc atgcactatc cctaaacccg ccctgtgcgg gtttttttac
     3661 gcctacgtgt aagtgtaaga gatgttatgg taaatcaggc tgacatggtc atcattttcc
     3721 ctgcagccta cccctctcct ctttcttgtt cagaacttaa tttatttgta tattttgtat
     3781 tttgttgtat tgttagttca ttgtgaagag atggggtaac actgatatga acgatatttc
     3841 tgacgtaagg cttaagaaaa caacggttga tagcctaaaa gaatacgtga atgactttgg
     3901 tatgacggtt gacgatgttg ttctgaactt aattgaagag aataattcat taaaatcgtt
     3961 attaggtcag gatttttctc tgcccagggg tgataaggac acgtatttat ttaaggaata
     4021 cgtgattcat aaactcccaa aaggaaagat cttggtaacc aaaaacggac tcaaggttag
     4081 taacatgaag aaggtattga tcgaggcagg cagcgcttta gggatggatg acaaagagct
     4141 aaaccctgac tttacaacct atgagatagg ccgttttctc ttcaaacacc ttcatggatg
     4201 aagatgtcta ccagttcgtg gccggggtgt tatggtaaat ctctgttggt gatatagaaa
     4261 ataatacctg cagcgcatta ccaattcaca ggtctgtcat gggctaaaca ttactagcga
     4321 aaaatgaagg tgacagatgt ttggattcgt aaaaaaagca gtcaacgcag tttcggatac
     4381 agcttcatct gcgttaagtt cagcttcaaa ggctgccaca agcgcctcgg catctatctc
     4441 agataaagcc tcatcaacat attcttctat ttctgattcg gctgagaaga ttacgggatc
     4501 agtatctggt gctgctcagt ctgcgtggtt aatggctgag tcagtcatta cagacggatt
     4561 gcttgattta acatctgatt ttcttaaaga cgatgagaaa atcagggagg tttcgctcat
     4621 tttctggcag aggataccaa gagaagttcg ctttttcctt tctgaagata aatttcatga
     4681 acgaacgaaa tcactaagag ataatgcttt gttgaagcta gatgaatcta agcaaaaaag
     4741 aattctgatt cctgaagcca caaacgcgga agacgcgata ccggttctta tctctaatga
     4801 accggcatca gtaacaactt tgcctgatgg tttacaagct aaaaattctc aggggtaaac
     4861 taccataacc gagcattgct ccaagacatc gaccgtagag gcctatcatg atttttatta
     4921 ttggtaagtg ctcatggtgg gtagtttgga gtacggaaat ttttgtacgt taaaaggatc
     4981 ttcttgagat ccatcatttg tcgtcgtagt ctcttgtcca agaaacgaaa aaaccacctg
     5041 gggaggtggt ttttcgaagg ttaagtcagt tggggaactg cttaacctgg taactggctt
     5101 cagcagagcg cagataccaa atactgtcct tccagtgtag ccgtagttag gccaccactt
     5161 caagaactct gtagcatcac ctacattaac tcgctctgct aatcctgtta ccaatggcta
     5221 ccgccactgg cgatttgtcg tgtctttccg ggttggactc aagttgatag ttaccggaac
     5281 tggcgtggta gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg
     5341 cctacccgga actgagtgtc aggcgtggaa tgagataaac gcggccataa cagcggaatg
     5401 acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccatc agggggaaac
     5461 gcctggtatc tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg
     5521 tgatgcttgt caggggggcg gagcctatgg aaaaagcgcc gctggcgcgg ccttctcttt
     5581 tttcgctcac atgttgtatg gagccttctc ccctgactct gtggatacat cgtgagcccg
     5641 ttgtgcgcgc gcaggcaacg cccgccgcag ccgaacggcc ggagcgtcag cgaccgagtg
     5701 agcgaggagg cgtcatatct ccggaacctc cattttctcc cgtacacccc gcggctgcat
     5761 ttcccctgcc acatgaagca ctttcataac gtattacccc accgtaacat attaagccag
     5821 tatacactcc gctagcgcta cgtgactggt tcagggctgc gccccgaaac ccgctaaaac
     5881 cactgacgcg cctgcggctt gtccaacacc gcgccgaccg ggaaaagatt tcccgcccgt
     5941 cccggcgtta tcaggaggcc ggattggcag acaaacgcag caaaatgctc acgatgtggg
     6001 tgaccgaaga tgagcaccgc cgtctgctgg agcgctgcga cggtaaacag cttgcagcct
     6061 ggatgcgcca gacgtgcctg gacgagaaac ccgcccgcgc cggcaagctt ccctcgctct
     6121 cgccggcgct gcttcgtcag cttgccggca tggggaacaa cctcaaccag attgcccggc
     6181 aggttaacgc cggtggtggc agcgggcgcg accgcgtgca ggtcgtcgcc gcgctgatgg
     6241 ccatagatgc cggactcgag cggctgcggc atgctgtgct gg
//
