LOCUS       NZ_CP103644             2495 bp    DNA     circular CON 20-SEP-2023
DEFINITION  Enterobacter hormaechei strain 4453 plasmid pMB5921_2, complete
            sequence.
ACCESSION   NZ_CP103644
VERSION     NZ_CP103644.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28174063
            Assembly: GCF_024917635.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 36x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/20/2023 05:52:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,823
            CDSs (total)                      :: 4,709
            Genes (coding)                    :: 4,602
            CDSs (with protein)               :: 4,602
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 33 of 107
            Pseudo Genes (incomplete)         :: 72 of 107
            Pseudo Genes (internal stop)      :: 19 of 107
            Pseudo Genes (multiple problems)  :: 15 of 107
            Pseudo Genes (short protein)      :: 1 of 107
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103644.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="4453"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="pMB5921_2"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2018-07-09"
                     /collected_by="MDACC"
                     /genotype="ST190"
     gene            1..84
                     /locus_tag="M5S55_RS24060"
                     /old_locus_tag="M5S55_24060"
     CDS             1..84
                     /locus_tag="M5S55_RS24060"
                     /old_locus_tag="M5S55_24060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032623649.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHD"
                     /name="hypothetical protein"
     gene            563..790
                     /locus_tag="M5S55_RS24065"
                     /old_locus_tag="M5S55_24065"
     CDS             563..790
                     /locus_tag="M5S55_RS24065"
                     /old_locus_tag="M5S55_24065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1156..1341
                     /locus_tag="M5S55_RS24070"
                     /old_locus_tag="M5S55_24070"
     CDS             1156..1341
                     /locus_tag="M5S55_RS24070"
                     /old_locus_tag="M5S55_24070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1632..1823)
                     /locus_tag="M5S55_RS24075"
                     /old_locus_tag="M5S55_24075"
     CDS             complement(1632..1823)
                     /locus_tag="M5S55_RS24075"
                     /old_locus_tag="M5S55_24075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021243041.1"
                     /translation="MTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQILSETEPAV
                     RTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     Typing          complement(1389..1476)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(2..91)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.604"
                     /coverage="100.0"
                     /evalue="1.95e-32"
     Typing          2243..2495
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.016"
                     /coverage="84.0"
                     /evalue="5.4099999999999996e-92"
ORIGIN
        1 atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcaacttgag
       61 tccaacccgg aaagacacga ctaatcgcca ttggcaacgg ccattggcaa acaggatgtg
      121 cgagagatgt ctcaagagtt cttgaagtgg cggccttagt gaggctacac tgaaaggaca
      181 aatttggtga catgtctcgc acaagactgt taccacggtt aggggatctg ccaggattac
      241 ctaaccttcg aaaaaccacc tgccagggtg gttttttcgt ttacagggca agagattacg
      301 cgcggaaaaa aaggatctca agaagatcct ttataaatta gacatccaac ctcacaaaga
      361 ggaatactta aatcatcttg gtatatgtat cggccggatt acatctcata taagcctcct
      421 ccccgcctga caccacactt tgaccatagc acctcgcgcc taactcgata tacagcccac
      481 catcaagcct cttttcacat aaaaaatgac ctatagttag atggttagtg accaaaactg
      541 atatggataa aaggagctgg ctttgaaaaa gatttttgtt tcagtgttcg cagcggcagt
      601 tgcgctttca gccctcaccg gctgcacgcg cacaagctat gccatacaca ccaatgacgg
      661 tcgcactatt gtcagcgacg gcaaacctaa agaatcagac tcaggcctgc ttggctatac
      721 agacgcgaat ggtgtcaaac agcagatcaa taaaaccgac gttaaagaag tctccgaaat
      781 tccgcactga gcatcttctc cgaaaaaggc cgctaatgtg gcctttttct ttacctgtgg
      841 ttctccgcca aaatcccagc aaattgcatc accaaagcta aaagctttca gggttgtcta
      901 aaaagcacaa gacataagag gaagtgcggt attttataat caagccccca agaatttacc
      961 ataacatccg ttgcccgcac cgcctgagac gcgttcagcg cgttcctgac gaaaccatga
     1021 caaaaaccac aacaaaccac cccggaaccc gtcagaaacg cgcctgttaa attttaacgg
     1081 catgcatgac tatgcaccag aatgacgcca tgctcttttc acgcaaaaat catcaccaga
     1141 cggggaaaat caccagtgac cagacaggaa tccgccgccc tcaatatggc caaatttatc
     1201 cgcgcacaga cacttctcct ccttgagcgg ctcgagcaga tggatctgga tgaggctgcc
     1261 ggctgctgtg agcacctgca cgatcaggcc gaagcgcttt acgccatgct gaacgcacag
     1321 ataggcgagg aaaatgcgtg aaaatcggtg aacgggtgcg caattcagtg cgcggccgtg
     1381 aggcgatggc ggggtgtcgg ggcgcagccc tgaccagggt atttgtgatg ccggcgcgtg
     1441 cgcggtatta caaatgcaca tcctgtcccg gaacggacac cgggaaacag caaaaaaaac
     1501 cgggcggcac gcccggaact caatcaagtt agattagatt actctcactc gtccataaca
     1561 gcatcatgga acgacgacca ccgtccgtga cggccgcctc gtttaagtat ggacagaaat
     1621 acagaaaatg ctcaggacga aatgtaatga atgcgaacgg attcaagaaa ttcgagcatg
     1681 acagtcctta cggccggttc ggtttcagac aaaatctgcc ggtatgcatc cagcatcatg
     1741 gctccggcat cccctccggc acgccgtagc cagaccgaaa caacggacac aagcaggtgt
     1801 cgctcatcat cactaagagt catcagggct ccggaagaaa aaccaaacat ggcttaccta
     1861 ctgttaacgg caacgcagaa gaaaggttta cagggtagcg ggcagggatg aaaaaaaaaa
     1921 aattgcagtc aggccggaac ggctgctgaa ccggtacagg cgggttccgg gcgcagcgcc
     1981 ggaccagtca cggagcacta gcggagtgta tactggctta agatgtagca tggcagtaac
     2041 acatagcatt aatgccacat aagacaggcg cagaaagtga aagaaaacgc gtcaggagaa
     2101 aatgtgatac agaatatatt ccgcttcctc gctcactgac tcgctccgct cggtcgttcg
     2161 gctgcggcga gcgggacggg cttacggatg gatcagaaat ttcctggggg atgccaggaa
     2221 gatacttaac gaagaaagca taaggccgcg ccaaagccgt ttttccatag gctccgcccc
     2281 cctgacaagc atcacagaat ctgacgctca aatcagtggt ggcgaaaccc gatgcggctt
     2341 aaagatacca ggcgtttccc cctggtggct ccctcgtgct cccccgcatt cctgcctttc
     2401 ggtttaccgg tgtcactccg ctgttatggc cgcggtttct cattccacgc ctgacactca
     2461 gttccgggaa ggcagttcgc tccaggatgg actgt
//
