LOCUS       NZ_CP103638             1748 bp    DNA     circular CON 20-SEP-2023
DEFINITION  Klebsiella pneumoniae strain 3221 plasmid pMB6013_3, complete
            sequence.
ACCESSION   NZ_CP103638
VERSION     NZ_CP103638.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28174025
            Assembly: GCF_024917555.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 127x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/20/2023 05:40:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,525
            CDSs (total)                      :: 5,399
            Genes (coding)                    :: 5,268
            CDSs (with protein)               :: 5,268
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 54 of 131
            Pseudo Genes (incomplete)         :: 76 of 131
            Pseudo Genes (internal stop)      :: 18 of 131
            Pseudo Genes (multiple problems)  :: 15 of 131
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103638.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="3221"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pMB6013_3"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2018-08-01"
                     /collected_by="MDACC"
                     /genotype="ST307"
     gene            1..990
                     /locus_tag="M5T10_RS27590"
                     /old_locus_tag="M5T10_27590"
     CDS             1..990
                     /locus_tag="M5T10_RS27590"
                     /old_locus_tag="M5T10_27590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_135628868.1"
                     /translation="MKNPELQNLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDSRWMFLTLTVRNCAIGDLGDTLSRMNTAFQRLKDRKEFRPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPGMLNGKSYVKHARWVELWRECLRVSYDPNVDVRAVKPRKPKDG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKIEVKKYRRSPTKDKTESE"
                     /name="protein rep"
     Typing          complement(719..908)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.859"
                     /coverage="99.0"
                     /evalue="3.7200000000000003e-85"
ORIGIN
        1 atgaaaaatc ctgaactcca gaacctcacg gactattccc cctcagatgc gccctgggac
       61 gctcacagaa gcgtttctga cgacgtggga gggatttacc tgcttgcggc cgaatatgag
      121 cgctacgggg cgcgtatggc ctcctgcggc ggtttactgc gtttcgggtg gtctaccctc
      181 aaagaaaccg gagaaacgcg cctgaggctg cgtgaggcgc atttctgccg tgtccggcac
      241 tgtcctgtct gccagtggag gcgctccctg atgtggcaag cccgtttcta ccagtccctg
      301 ccgcgtatcg tggcggatta ccccgattcc cgctggatgt tcctgaccct gaccgtgcgc
      361 aactgtgcga tcggggatct gggcgatacg ctgagccgga tgaacacggc tttccagcgg
      421 ctgaaggatc gcaaagagtt cagaccagtt cagggatgga tccgcaccac ggaagtcacc
      481 cgcggcagtg acggcagcgc acacccgcac ttccacacac tgatgatggt tccgcctggc
      541 atgcttaacg ggaagagtta cgtcaaacat gcgcgctggg ttgagctgtg gcgggagtgc
      601 cttcgggtca gctacgatcc gaatgtcgat gtccgggcag tgaaaccccg gaagccgaag
      661 gacggcgaat cgctggcctg cgcgaccgcc gaactggtgc gcggcgcggt ggccgagacc
      721 ctgaaatatt ccacgaagcc ggccgacatg gtcgccgatc ctgaatggtt cctcgaactg
      781 acccgccaga cgcacaagcg ccgctttgtg gcgaccggcg gcgcactcaa agacgtattg
      841 aaactcgatc aggaaaccga tgccgacatg gtgatcgggg atgatatttc cgagggcgat
      901 gatgacggtt cccgcatcgc gttcgagtgg aaaatcgagg tcaaaaaata ccggcgttcg
      961 ccgacgaaag ataaaaccga atcagagtaa aaacactgcg atgcaggata gggcaaacgc
     1021 cgcaaaatga cgtcatccga catcattccg ccgcgttcgc ccagtgacct gcagtcggct
     1081 ctggggtgtc ggggctgcgc cctgacctgg ggttttggat tgcggccatc gtgatggcgg
     1141 taatacaaaa cgcacgggac aagatgtgtt tttggagtac cgccgacacg cgacggacag
     1201 tccaaaatat cttggtcagg gctgcgcccc gacaccctgc caacacctca cgccgccaga
     1261 ttaaaatctg ccagcgttcg ccgttcctga aatcgtcaaa cgctaaatcg tggggtcccg
     1321 ctggcttgcc agtgggttcc gatttagtga ttgacgagcg cacccgtaaa acactgccga
     1381 caggtggttg tgatcacgca gtgagcgcaa ccacctgata agcgtttttg gggtttgtgg
     1441 ggtccgattt aacgtggatt ttgggggcaa aaaatcgcgc gcgttccttg tatttatact
     1501 taagggataa ctctggcgtt aaaaataagc ctcagccctt gtgccgcaag ggtttcagcg
     1561 tgatttgcct gaaaatgact ttgaagttat tctgtctgga gtggtcgcgt taaaagcgac
     1621 ccccgcaacg acggccatcg ttcgggggcg tgaccacctg taggaattct attttgcgac
     1681 agacaacaca ggtgatacgg caatgataac acgtcaagtg agccatcgcc cagtatgggg
     1741 cggtgcgc
//
