LOCUS       NZ_CP103629            10209 bp    DNA     circular CON 21-SEP-2023
DEFINITION  Escherichia coli strain 4012 plasmid pMB6206_3, complete sequence.
ACCESSION   NZ_CP103629
VERSION     NZ_CP103629.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28174046
            Assembly: GCF_024918275.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10209)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 10209)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 273x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/21/2023 03:21:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,007
            CDSs (total)                      :: 4,882
            Genes (coding)                    :: 4,698
            CDSs (with protein)               :: 4,698
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 67 of 184
            Pseudo Genes (incomplete)         :: 116 of 184
            Pseudo Genes (internal stop)      :: 32 of 184
            Pseudo Genes (multiple problems)  :: 33 of 184
            Pseudo Genes (short protein)      :: 4 of 184
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103629.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="4012"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMB6206_3"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2018-08-21"
                     /collected_by="MDACC"
                     /genotype="ST90"
     gene            1..525
                     /gene="cni"
                     /locus_tag="M5S78_RS24805"
                     /old_locus_tag="M5S78_24805"
     CDS             1..525
                     /gene="cni"
                     /locus_tag="M5S78_RS24805"
                     /old_locus_tag="M5S78_24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_061352107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin N immunity protein"
                     /protein_id="WP_040089860.1"
                     /translation="MDIKDRNKISKKISFSLLLLLSPFALIFFSYNNAPIPLLEKIIAY
                     LSLPGFHSSNNPPLSEAFNLYVHTAPLAAISLFIFTHKELELKPKSSSLRALKILTPFT
                     ILYISMIYCFLLTDTELTLSSKTFVLMSKNDLFLSFFYITLYIGIYIFTYLYLWFFIGT
                     YKLFTRGGILP"
                     /name="cni"
     gene            804..2066
                     /locus_tag="M5S78_RS24810"
                     /old_locus_tag="M5S78_24810"
     CDS             804..2066
                     /locus_tag="M5S78_RS24810"
                     /old_locus_tag="M5S78_24810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISEcp1 family transposase"
                     /protein_id="WP_000608644.1"
                     /translation="MINKIDFKAKNLTSNAGLFLLLENAKSNGIFDFIENDLVFDNDST
                     NKIKMNHIKTMLCGHFIGIDKLERLKLLQNDPLVNEFDISVKEPETVSRFLGNFNFKTT
                     QMFRDINFKVFKKLLTKSKLTSITIDIDSSVINVEGHQEGASKGYNPKKLGNRCYNIQF
                     AFCDELKAYVTGFVRSGNTYTANGAAEMIKEIVANIKSDDLEILFRMDSGYFDEKIIET
                     IESLGCKYLIKAKSYSTLTSQATNSSIVFVKGEEGRETTELYTKLVKWEKDRRFVVSRV
                     LKPEKERAQLSLLEGSEYDYFFFVTNTTLLSEKVVIYYEKRGNAENYIKEAKYDMAVGH
                     LLLKSFWANEAVFQMMMLSYNLFLLFKFDSLDSSEYRQQIKTFRLKYVFLAAKIIKTAR
                     YVIMKLSENYPYKGVYEKCLV"
                     /name="IS1380-like element ISEcp1 family transposase"
     gene            2322..3197
                     /locus_tag="M5S78_RS24815"
                     /old_locus_tag="M5S78_24815"
     CDS             2322..3197
                     /locus_tag="M5S78_RS24815"
                     /old_locus_tag="M5S78_24815"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extended-spectrum class A beta-lactamase
                     CTX-M-55"
                     /protein_id="WP_015387340.1"
                     /translation="MVKKSLRQFTLMATATVTLLLGSVPLYAQTADVQQKLAELERQSG
                     GRLGVALINTADNSQILYRADERFAMCSTSKVMAVAAVLKKSESEPNLLNQRVEIKKSD
                     LVNYNPIAEKHVNGTMSLAELSAAALQYSDNVAMNKLIAHVGGPASVTAFARQLGDETF
                     RLDRTEPTLNTAIPGDPRDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNTTGAA
                     SIQAGLPASWVVGDKTGSGGYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESRRDVLA
                     SAAKIVTDGL"
                     /name="extended-spectrum class A beta-lactamase CTX-M-55"
     gene            complement(3244..>3591)
                     /locus_tag="M5S78_RS24820"
                     /old_locus_tag="M5S78_24820"
                     /pseudo=""
     CDS             complement(3244..>3591)
                     /locus_tag="M5S78_RS24820"
                     /old_locus_tag="M5S78_24820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016943399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /name="WbuC family cupin fold metalloprotein"
     gene            complement(3528..4673)
                     /gene="cna"
                     /locus_tag="M5S78_RS24825"
                     /old_locus_tag="M5S78_24825"
     CDS             complement(3528..4673)
                     /gene="cna"
                     /locus_tag="M5S78_RS24825"
                     /old_locus_tag="M5S78_24825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin colicin N"
                     /protein_id="WP_310795390.1"
                     /translation="MGSNGADNAHNNAFGGGKNPGIGNTSGAGSNGSASSNRGNSNGWS
                     WSNKPHKNDGFHSDGSYHITFHGDNNSKPKPGGNSGNRGNNGDGSSAKAGEITITPDNS
                     KPGRYISSNPEYSLLAKLIDAESIKGTEVYTFHTRKGQYVKVTVPDSNIDKMRVDYVNW
                     KGPKYNNKLVKRFVSQFLLFRKEEKEKNEKEALLKASELVSGMGDKLGEYLGEKYKNVA
                     KEVAGDIKNFHGRNIRSYNEAMASLNKVLANPKMKVNKSDKDAIVNAWKQVNAQDMANK
                     IGNLGKAFKVADLAIKVEKIREKSIEGYNTGNWGPLLLEVESWIIGGVVAGVAISLFGA
                     VLSFLPISGLAVTVLGVIGIMTLGPTCARTDTLTPSSYCCH"
                     /name="cna"
     gene            4933..5370
                     /locus_tag="M5S78_RS24830"
                     /old_locus_tag="M5S78_24830"
     CDS             4933..5370
                     /locus_tag="M5S78_RS24830"
                     /old_locus_tag="M5S78_24830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001749514.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKQNETQERHTVGQAENPHDQQTERILRELNELKQYLTLMLEDKQA
                     QDMDRRHQEAEREQLQNEIAQLRQALEQEKKRGFWSRLFGH"
                     /name="hypothetical protein"
     gene            5451..5879
                     /locus_tag="M5S78_RS24835"
                     /old_locus_tag="M5S78_24835"
     CDS             5451..5879
                     /locus_tag="M5S78_RS24835"
                     /old_locus_tag="M5S78_24835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001749513.1"
                     /translation="MKRSGGTRYLYLISLITVAAALTACTPKGSVEQHTRHYVYASDDR
                     SDPNFYTNKADTTRMMIPFFQQFREMGEKDKAAGVSAETAQQRIKEFHSEKFLQSLRST
                     TTFAGRKYMNSDMPSPEKMKLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            5979..6281
                     /locus_tag="M5S78_RS24840"
                     /old_locus_tag="M5S78_24840"
     CDS             5979..6281
                     /locus_tag="M5S78_RS24840"
                     /old_locus_tag="M5S78_24840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024139129.1"
                     /translation="MLKTVRSCLLSALLTHLFFGNIAVAKENLSDDKVKQAIIDESIAA
                     YPGTCACPFNSARNGSSCGRRSAWSKAGGYSPICYKKEVTKEMIKDWRASHSNNN"
                     /name="hypothetical protein"
     gene            6373..6687
                     /locus_tag="M5S78_RS24845"
                     /old_locus_tag="M5S78_24845"
     CDS             6373..6687
                     /locus_tag="M5S78_RS24845"
                     /old_locus_tag="M5S78_24845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024139128.1"
                     /translation="MSNNYDLIAKAIKEKLQVTAYYQGFYREMCPHALGSKKGRKQALF
                     YQFGGESSKGTVTPGSTFNWRCIPIDGLTEVTLQSGQWYTAENHSQAQTCVDQIDIEVQ
                     "
                     /name="hypothetical protein"
     gene            complement(7067..7300)
                     /locus_tag="M5S78_RS24850"
                     /old_locus_tag="M5S78_24850"
     CDS             complement(7067..7300)
                     /locus_tag="M5S78_RS24850"
                     /old_locus_tag="M5S78_24850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_004026237.1"
                     /translation="MTELEIHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(7307..7825)
                     /locus_tag="M5S78_RS24855"
                     /old_locus_tag="M5S78_24855"
     CDS             complement(7307..7825)
                     /locus_tag="M5S78_RS24855"
                     /old_locus_tag="M5S78_24855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001749510.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<7863..8561)
                     /locus_tag="M5S78_RS25065"
                     /pseudo=""
     CDS             complement(<7863..8561)
                     /locus_tag="M5S78_RS25065"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(8503..8826)
                     /locus_tag="M5S78_RS24865"
                     /old_locus_tag="M5S78_24865"
     CDS             complement(8503..8826)
                     /locus_tag="M5S78_RS24865"
                     /old_locus_tag="M5S78_24865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001430281.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            8890..9114
                     /locus_tag="M5S78_RS24870"
                     /old_locus_tag="M5S78_24870"
     CDS             8890..9114
                     /locus_tag="M5S78_RS24870"
                     /old_locus_tag="M5S78_24870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_089537792.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENLLTSPSGRVSRF"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            9695..9856
                     /locus_tag="M5S78_RS25070"
     CDS             9695..9856
                     /locus_tag="M5S78_RS25070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596883.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRARFI
                     QMLTEFLK"
                     /name="replication initiation protein"
     gene            complement(10045..10182)
                     /locus_tag="M5S78_RS24875"
                     /old_locus_tag="M5S78_24875"
     CDS             complement(10045..10182)
                     /locus_tag="M5S78_RS24875"
                     /old_locus_tag="M5S78_24875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329514.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_024139133.1"
                     /translation="MCGKILLILFFIMTLSACQVNHIRDVKGGTVAPSSSSRLTGVEIK
                     "
                     /name="colicin release lysis protein"
     ARG             2323..3194
                     /name="blaCTX-M-55"
                     /gene="blaCTX-M-55"
                     /gene_name="extended-spectrum class A beta-lactamase
                     CTX-M-55"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(9111..9198)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          9222..9767
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.536"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(6964..8513)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.374"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggatataa aagacagaaa taagatatca aaaaaaatat cattcagtct tctgctctta
       61 ctttccccat tcgcattaat atttttcagt tataataatg caccaatacc actccttgaa
      121 aaaatcatcg catacctatc cctaccagga tttcattcat caaacaaccc gcccctaagc
      181 gaagcattca atctctatgt tcatacagcc cctttagctg caatcagctt attcatattc
      241 acacacaaag aattagagtt aaaaccaaag tcatcatctc tgcgggcgct aaagatatta
      301 actcctttca ctattcttta tatatccatg atatactgtt tcttgctaac tgacacagaa
      361 ctaaccttgt catcaaaaac atttgtatta atgtcaaaaa atgacctgtt tttgtctttc
      421 ttctatataa cactatacat tgggatatat atattcacat atttatacct ttggttcttt
      481 ataggaacat ataaactatt taccagggga ggtatactcc cctgacactc atgtcatcga
      541 ataacactag atataatgtt atttatattc gaaactcgat ttgcatcaat gaaagatgac
      601 aagtaactaa tcgtcatcct agattctacg tcagtacttc aaaaagcata atcaaagcct
      661 tgataaatat gcattccttc gaaattcagc tttcacccat tgggtgaaag aaaagtgctc
      721 aaaaatatgt taaattatca gcttttatga ctcgatatat ggtaaaataa tagtaagaaa
      781 agtagtaaaa aggggttcta attatgatta ataaaattga tttcaaagct aagaatctaa
      841 catcaaatgc aggtcttttt ctgctccttg agaatgcaaa aagcaatggg atttttgatt
      901 ttattgaaaa tgacctcgta tttgataatg actcaacaaa taaaatcaag atgaatcata
      961 taaagaccat gctctgcggt cacttcattg gcattgataa gttagaacgt ctaaagctac
     1021 ttcaaaatga tcccctcgtc aacgagtttg atatttccgt aaaagaacct gaaacagtgt
     1081 cacggtttct aggaaacttc aacttcaaga caacccaaat gtttagagac attaatttta
     1141 aagtctttaa aaaactgctc actaaaagta aattgacatc cattacgatt gatattgata
     1201 gtagtgtaat taacgtagaa ggtcatcaag aaggtgcgtc aaaaggatat aatcctaaga
     1261 aactgggaaa ccgatgctac aatatccaat ttgcattttg cgacgaatta aaagcatatg
     1321 ttaccggatt tgtaagaagt ggcaatactt acactgcaaa cggtgctgcg gaaatgatca
     1381 aagaaattgt tgctaacatc aaatcagacg atttagaaat tttatttcga atggatagtg
     1441 gctactttga tgaaaaaatt atcgaaacga tagaatctct tggatgcaaa tatttaatta
     1501 aagccaaaag ttattctaca ctcacctcac aagcaacgaa ttcatcaatt gtattcgtta
     1561 aaggagaaga aggtagagaa actacagaac tgtatacaaa attagttaaa tgggaaaaag
     1621 acagaagatt tgtcgtatct cgcgtactga aaccagaaaa agaaagagca caattatcac
     1681 ttttagaagg ttccgaatac gactactttt tctttgtaac aaatactacc ttgctttctg
     1741 aaaaagtagt tatatactat gaaaagcgtg gtaatgctga aaactatatc aaagaagcca
     1801 aatacgacat ggcggtgggt catctcttgc taaagtcatt ttgggcgaat gaagccgtgt
     1861 ttcaaatgat gatgctttca tataacctat ttttgttgtt caagtttgat tccttggact
     1921 cttcagaata cagacagcaa ataaagacct ttcgtttgaa gtatgtattt cttgcagcaa
     1981 aaataatcaa aaccgcaaga tatgtaatca tgaagttgtc ggaaaactat ccgtacaagg
     2041 gagtgtatga aaaatgtctg gtataataag aatatcatca ataaaattga gtgttgctct
     2101 gtggataact tgcagagttt attaagtatc attgcagcaa agatgaaatc aatgatttat
     2161 caaaaatgat tgaaaggtgg ttgtaaataa tgttacaatg tgtgagaagc agtctaaatt
     2221 cttcgtgaaa tagtgatttt tgaagctaat aaaaaacaca cgtggaattt agggactatt
     2281 catgttgttg ttatttcgta tcttccagaa taaggaatcc catggttaaa aaatcactgc
     2341 gccagttcac gctgatggcg acggcaaccg tcacgctgtt gttaggaagt gtgccgctgt
     2401 atgcgcaaac ggcggacgta cagcaaaaac ttgccgaatt agagcggcag tcgggaggca
     2461 gactgggtgt ggcattgatt aacacagcag ataattcgca aatactttat cgtgctgatg
     2521 agcgctttgc gatgtgcagc accagtaaag tgatggccgt ggccgcggtg ctgaagaaaa
     2581 gtgaaagcga accgaatctg ttaaatcagc gagttgagat caaaaaatct gaccttgtta
     2641 actataatcc gattgcggaa aagcacgtca atgggacgat gtcactggct gagcttagcg
     2701 cggccgcgct acagtacagc gataacgtgg cgatgaataa gctgattgct cacgttggcg
     2761 gcccggctag cgtcaccgcg ttcgcccgac agctgggaga cgaaacgttc cgtctcgacc
     2821 gtaccgagcc gacgttaaac accgccattc cgggcgatcc gcgtgatacc acttcacctc
     2881 gggcaatggc gcaaactctg cggaatctga cgctgggtaa agcattgggc gacagccaac
     2941 gggcgcagct ggtgacatgg atgaaaggca ataccaccgg tgcagcgagc attcaggctg
     3001 gactgcctgc ttcctgggtt gtgggggata aaaccggcag cggtggctat ggcaccacca
     3061 acgatatcgc ggtgatctgg ccaaaagatc gtgcgccgct gattctggtc acttacttca
     3121 cccagcctca acctaaggca gaaagccgtc gcgatgtatt agcgtcggcg gctaaaatcg
     3181 tcaccgacgg tttgtaatag cggaaacggg atggggaaac tcattccgtt tttgtttatc
     3241 gccttagacg gcaaaagtgc tgtcgcccac ctgcgcttgc gcataccagg ccataagctc
     3301 cgtggttcct ggttctcctt ccgctggagc ccagtgcgca tagtcatcgg cagccacggg
     3361 ttgatagcca ccgtgtttta cttcaaaaat tatgccaccg gtatccagcg acagcacggc
     3421 atgccaggtt cctgcggcca tctccagcac cgtacaggtt tcccccaata tcgcccgatg
     3481 ggtgacggta ccccgatcgt caaaattcag caccacgaaa cgacccctta atggcaacag
     3541 tagctcgaag gtgtgagggt gtcggtgcgg gcgcacgtag gtcccagggt cattattcct
     3601 attaccccca acacagtaac agcaagtcct gagattggga gaaaactcaa cacagcccca
     3661 aacaaactaa tagcaactcc agcaacaacg cccccgatga tccatgattc aacctccagc
     3721 aggagaggtc cccagttgcc agtattgtat ccctcaatgc ttttttccct aattttctca
     3781 acctttatag ctaaatcagc aaccttaaat gccttgccaa gattaccaat cttattagcc
     3841 atgtcctgag cattaacctg tttccaggca ttcacaatgg catccttatc agatttgttt
     3901 actttcatct ttggatttgc taacacttta ttcagtgaag ccattgcttc attatagcta
     3961 cgaatattac ggccatgaaa gtttttaata tcaccggcaa cttccttagc tacattttta
     4021 tatttttctc caagatactc gccaagttta tcccccatac cagacacaag ttcactagcc
     4081 tttagcaagg cttctttttc atttttttct ttttcttcct tcctaaataa taaaaactgg
     4141 ctcacaaacc tcttcacaag tttattgtta tatttcggtc ccttccagtt cacataatca
     4201 actctcattt tatcaatatt actatctgga acagtaacct tgacatactg accttttctg
     4261 gtgtgaaaag tatatacctc tgtaccttta attgattccg catcaattaa ttttgctaac
     4321 aatgaatatt ctggatttga cgaaatataa cgaccaggtt tcgagttgtc aggtgtaatt
     4381 gttatctctc cagccttagc actcgatcca tcaccattat tacctctatt tccactattc
     4441 ccaccaggtt taggctttga attattgtcc ccatgaaatg taatatggta agaaccatca
     4501 ctgtggaagc catcattttt atgaggctta ttactccatg accatccatt ggaatttcct
     4561 cggttacttg atgcgctacc attacttcca gcgccactgg tattaccaat gcccggattt
     4621 ttccctccac caaaagcatt gttatgtgcg ttatctgcgc cattactacc cataataaac
     4681 ccctctttga cttttaaaac aaaccattaa aaacaaatac tgtacatata accactggtt
     4741 ttatgtacag tataaaagct acgccgctgc attttccctg tcaagactgt ggacttccat
     4801 ttttgtgaaa acgatcaaaa aaacagtctc tcacaccacg cgctattccc gccggatacc
     4861 acaaaaaccg gcgcaaacat taccgttctc agacctcatt atgttttact gaaaccatga
     4921 gatgagacac ctatgggaca ctgtaacttt atggcatggc acacactccg ggacgcactg
     4981 aaaatgactg gaagatcgcg ttcacagttt taccgtgata tgcgctcagg tcttgtcagt
     5041 taccgtacca gccaggacgg acgaagggag tttgaaacca gtgaactgat ccgggcatac
     5101 ggcgaattaa agcaaaatga gacacaagaa aggcacactg tgggacaggc agaaaatcca
     5161 catgatcagc agacagaacg cattctccgg gaactgaatg agctgaaaca atacctgacg
     5221 ctgatgcttg aggataaaca ggcacaggat atggatcgca ggcaccagga agcagaacgg
     5281 gaacagttac agaatgagat agcccagctc aggcaggcac tggaacagga aaagaaacgg
     5341 ggattctggt ccagactctt cggtcactga actcggtcag aggctgatga taagatagtc
     5401 ttcggataat aactcaccga gaataaatac tttaaggtaa ggagacacgt atgaaacgtt
     5461 ctggtggcac acgctatctg tatcttatat ccctgataac tgtggctgca gccctcacgg
     5521 cctgtacccc gaaaggcagt gtggaacaac atacccgaca ttatgtgtac gcttccgatg
     5581 accgttctga ccctaacttc tacaccaaca aggccgacac cacacggatg atgattccgt
     5641 tctttcagca gttcagggag atgggggaga aagacaaagc agccggagta tcagcagaaa
     5701 cggcacagca acgcataaaa gaattccact cagaaaaatt tcttcagtct ctcagaagca
     5761 caactacctt tgcgggcaga aaatacatga atagcgatat gccatcaccg gaaaaaatga
     5821 agctgctggc tgacacgatt tctgcggttt atctcgatgg ctacgagggc agacagtaag
     5881 tggatttacc ataatccctt aacaacaaga agcgatttaa gttgtttttt tgtacaaaaa
     5941 tgcaattctc ctaggattat tcctcggaga ctattcaaat gcttaagaca gtacgttcgt
     6001 gtctgttatc agctttactg acacatcttt ttttcggcaa tatcgctgtg gcgaaagaaa
     6061 atctatcaga tgacaaagta aagcaggcga ttattgacga gtcgatagca gcctatcccg
     6121 gaacatgtgc atgtccgttt aacagcgcaa gaaatggcag ctcgtgcggc aggcgcagcg
     6181 cctggagcaa agcaggaggt tattcgccaa tctgctacaa aaaagaggta acaaaagaaa
     6241 tgataaaaga ttggcgagct tctcactcaa ataacaactg acttctattt ttataacttt
     6301 ctgtttacat tacatttctg ataaatacat atatttccaa caggaaatca tactctggga
     6361 gattgaagct aagtgtctaa taattatgat ttaattgcca aggcaatcaa agaaaagttg
     6421 caagtaacag catattacca aggtttttac cgcgagatgt gccctcatgc gctaggttca
     6481 aaaaaaggac gaaaacaggc tcttttctat cagtttggcg gtgaaagcag taaaggtact
     6541 gtgacacctg gttccacatt caactggcgc tgcattccta tagatggtct aactgaggtc
     6601 accttacaat caggccagtg gtacacagca gaaaatcaca gccaagcaca gacttgtgtt
     6661 gatcagatcg atattgaagt tcaataaagc ctgtattcgt accctcataa agctgccatt
     6721 agtggcagct ttatgtttat gacaaccatc agcatttaac ataaagccct gattgtacgc
     6781 accgctaaaa cgcgttcagc gcgatcacgg cagcaaacag gtaaaaatgg caacaaacca
     6841 cccgaaaaac tgccgcgatc gcgcctgata aattttaacc gtatgaatac ctatgcaacc
     6901 agatggtaca ggccacattc cccccactta atccactgaa gctgccattt ttcatggttt
     6961 caccatccca gcgaagggcc atccagcgtg cgttcctgta tttccggctg acgctcccgt
     7021 tccagggata acacatgttc gcgctcctgt atcaaccgtt cctctcttat ctccagttct
     7081 cgctgtataa ctggctcaag cgttctgtct gctcgctcaa gtgctgcacc tgctgactca
     7141 actgcatgac ccgctcgttc agcatcgcgt tgtcccgttg cgtaagcgaa aacatcttct
     7201 gcaattccac gaaggcgctc tcccattcgc tcagccgctg catatagtcc tgttgcagct
     7261 gctctaaggc gttcagcaaa tgtatttcca gctctgtcac tctgtgtcac tccttcagat
     7321 gtacccactc tttcccctga aagggaatca cctccgctga tttcccgtac ggaaggacaa
     7381 ggaatttcct gttcccgtcc tgcacaaact ccacgcccca tgtcttcgcg ttcagtttct
     7441 gcaatgtctc ttcctgcttc ctgatttctt ccaggttcgt ctgtatccgt ccgccaagat
     7501 accagagcgt cccgccactc gcggtaaaca ggagaaagac tatccccagt aacatcatgc
     7561 ccgtattccc tgccagcttt aacacgtcct tcctgtgctg catcatcgcc tctttcaccc
     7621 cttcccggtg tttttccagc gatttctctg tcgaggctgt gaacagggct atagcgtctc
     7681 tgattttcgt ctcgtttgag gtcacagcct cgctgacaga ctcgccgagc ttcctgaact
     7741 cgtcgttcag cattttctct gtagattcgg ctctctcttt cagctttttc tcgaactccg
     7801 cgcccgtctg taaaagattg ctcataaaat gctcctttca gcctgatatt cctcccgccg
     7861 ttcgggtctg caatgctgat actgcttcgc gtcaccctga tcacttcaag ccccgcctct
     7921 gtgagcgcct gaatcacatc ctgacggcct tttatctctc cggcatggta aagtgcgtct
     7981 ataccgcgtg taacgctctc agcaagtgcc tgtttcgttt ccggcaggtt atcaggaagg
     8041 gtcagcgtcc ggcggttctc cggggcgttg gggtcatgca gtccgtaatg gtgattcacc
     8101 agcgtctgcc aggcatcaat tctcggcctg tctgcgcggt cgtagtacgg ctggagacgt
     8161 tttccggtct gtagctccat gttcggaatg acaaaattca gctcaagccg tcccttgtcc
     8221 tggtgctcca cccacaggat gctgtactga tttttttcga gaccgggcat cagtacacgc
     8281 tcaaagctcg ccatcacttt ttcacgtcct cccggcggca gttccttctc cgcgaacgac
     8341 agaacaccgg acgtgtattt cttcgcaaat ggcgtggcat cgatgagttc ccggacttct
     8401 tccggattac cctgaagcac cgttgcgcct tcgcggttac gctccctccc cagcaggtaa
     8461 tcaaccggac cactgccacc accttttccc ctggcatgaa atttaactat catcccgcgc
     8521 cccctgttcc ctgacggcca gacgcagccg gcgcagctca tccccgatgg ccatcagtgc
     8581 ggccaccacc tgaacccggt caccggaaga ccactgcccg ctgttcacct tacgggctgt
     8641 ctgattcagg ttatttccga tggcggccag ctgacgcagt aacggcggtg ccagtgtcgg
     8701 cagcctcccg gaacgggcaa ccggctcccc caggcagacc cgccgcatcc ataccgccag
     8761 ttgtttaccc tcacagcgtt caagtaaccg ggcatgttca tcatcagtaa cccgtatcgt
     8821 gagcatcctc tcgcgtttca tcggtatcat taccccatga acagaaatcc cccttacacg
     8881 gaggcatcag tgactaaaca ggaaaaaaca gcccttaaca tggcgcgctt tatcagaagc
     8941 cagacattaa cgctgctgga aaagctcaac gaactggacg cggatgaaca ggcagacatc
     9001 tgtgaatcgc ttcacgacca cgccgatgag ctttaccgca gctgcctcgc acgtttcggg
     9061 gatgacggtg aaaacctgct gacaagcccg tcagggcgcg tcagcaggtt ttagcgggtg
     9121 tcggggcgca gccctgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat
     9181 gcggcatcag tgcggattgt atgaaaagca cgccatgccg ggtgtgaaat gccgcacaga
     9241 tgcgtaagga gaaaatgcag cttccgacgc tcttccgctt cctcgctcac tgactcgctg
     9301 cgctcggtcg ttcggctgcg gcaagcggtg ccggctcact caaaggcgga aatacggtta
     9361 tccacagaat caggggataa ggccggaaag aacatgtgag taaaaaggca aagggcaaca
     9421 aaaggccgcg ccgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     9481 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     9541 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     9601 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgtt ggtatctcgg
     9661 cccggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     9721 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgccttaac
     9781 gccactggca gcagccactg gtaaccggat tagcagagcg agatttatcc agatgcttac
     9841 agagttcttg aagtgatggc ccaactgcgg ctacactgaa aggacagtat ttggtatctg
     9901 cgctctgctg aagccagtta cctcggttaa gcagtcccca actgacttaa ccttcgacta
     9961 aaccgcctcc ccaggcggtt ttttcgttta caggcagcag attacgcgca gaaaaaaagg
    10021 atctcaagag gatcctttga tcttttactt aatttcaact cctgttagtc tggatgatga
    10081 tgatggagca acagtccctc ctttcacatc acggatgtga ttgacctgac atgcactaag
    10141 tgtcatgata aaaaacaaga tcaatagaat tttcccacac ataatttcca accatccaaa
    10201 gaaagcaac
//
