LOCUS       NZ_CP103620            10123 bp    DNA     circular CON 20-SEP-2023
DEFINITION  Klebsiella pneumoniae strain 4383 plasmid pMB6487_2, complete
            sequence.
ACCESSION   NZ_CP103620
VERSION     NZ_CP103620.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28174058
            Assembly: GCF_024917595.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10123)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 10123)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 153x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/20/2023 05:38:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,493
            CDSs (total)                      :: 5,369
            Genes (coding)                    :: 5,218
            CDSs (with protein)               :: 5,218
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 56 of 151
            Pseudo Genes (incomplete)         :: 96 of 151
            Pseudo Genes (internal stop)      :: 26 of 151
            Pseudo Genes (multiple problems)  :: 24 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103620.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10123
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="4383"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pMB6487_2"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2018-09-16"
                     /collected_by="MDACC"
                     /genotype="ST15"
     gene            1..180
                     /locus_tag="M5T08_RS27365"
                     /old_locus_tag="M5T08_27365"
     CDS             1..180
                     /locus_tag="M5T08_RS27365"
                     /old_locus_tag="M5T08_27365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021515244.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(208..738)
                     /locus_tag="M5T08_RS27370"
                     /old_locus_tag="M5T08_27370"
     CDS             complement(208..738)
                     /locus_tag="M5T08_RS27370"
                     /old_locus_tag="M5T08_27370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021515243.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLSDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPSGKK"
                     /name="hypothetical protein"
     gene            complement(745..1476)
                     /gene="mobC"
                     /locus_tag="M5T08_RS27375"
                     /old_locus_tag="M5T08_27375"
     CDS             complement(745..1476)
                     /gene="mobC"
                     /locus_tag="M5T08_RS27375"
                     /old_locus_tag="M5T08_27375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004146447.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDIIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGATGWRYLHRIALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(1476..3437)
                     /locus_tag="M5T08_RS27380"
                     /old_locus_tag="M5T08_27380"
     CDS             complement(1476..3437)
                     /locus_tag="M5T08_RS27380"
                     /old_locus_tag="M5T08_27380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_021515242.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPNFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QPEARRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(4923..5072)
                     /locus_tag="M5T08_RS27385"
                     /old_locus_tag="M5T08_27385"
     CDS             complement(4923..5072)
                     /locus_tag="M5T08_RS27385"
                     /old_locus_tag="M5T08_27385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_010891188.1"
                     /translation="MKKAKAIFLFILIVSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(5157..5414)
                     /locus_tag="M5T08_RS27390"
                     /old_locus_tag="M5T08_27390"
     CDS             complement(5157..5414)
                     /locus_tag="M5T08_RS27390"
                     /old_locus_tag="M5T08_27390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_010891189.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKPWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(5424..7109)
                     /locus_tag="M5T08_RS27395"
                     /old_locus_tag="M5T08_27395"
     CDS             complement(5424..7109)
                     /locus_tag="M5T08_RS27395"
                     /old_locus_tag="M5T08_27395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032427631.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSFSGDALSSAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADTVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPALQVSVPKGVPAAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNITTLNGRITSTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNAKLKAAQASLNAMNDALSRDKAALSGAMESRKQKEKKAKEAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            7436..7681
                     /locus_tag="M5T08_RS27400"
                     /old_locus_tag="M5T08_27400"
     CDS             7436..7681
                     /locus_tag="M5T08_RS27400"
                     /old_locus_tag="M5T08_27400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021515249.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEAAETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            7803..8369
                     /locus_tag="M5T08_RS27405"
                     /old_locus_tag="M5T08_27405"
     CDS             7803..8369
                     /locus_tag="M5T08_RS27405"
                     /old_locus_tag="M5T08_27405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008812849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein K"
                     /protein_id="WP_010891191.1"
                     /translation="MDKRTTLVALVSIIFFTSGCDDQKKGNGNAESTQITKNDITTQLK
                     LDKTQQPDDIEKKIKEIQANSTPEKIKKFNELKDDDPKILRESVVSEIKKKLPLLVDEA
                     TLMTDVSTDGGTFSYKYVTKGISASTMESDVWKDAMQKNIKNSYCSDDARLKVFRELFP
                     EGVIYNYYLSDKLIYTYKALPSICS"
                     /name="protein K"
     gene            8455..8718
                     /locus_tag="M5T08_RS27410"
                     /old_locus_tag="M5T08_27410"
     CDS             8455..8718
                     /locus_tag="M5T08_RS27410"
                     /old_locus_tag="M5T08_27410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004749445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="WP_010891192.1"
                     /translation="MAAYTSETAKFLQSVSSDQTWWKSRYNPGDSVPHSGIYRCTVCGK
                     EITSNGNDPFPPQNHHQHSQQQAIKWQLIVRTDTKGDRFGIK"
                     /name="protein L"
     gene            9064..9327
                     /locus_tag="M5T08_RS27415"
                     /old_locus_tag="M5T08_27415"
     CDS             9064..9327
                     /locus_tag="M5T08_RS27415"
                     /old_locus_tag="M5T08_27415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021515247.1"
                     /translation="MSNTTHYENANFLRELAENLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRSGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            9317..9616
                     /locus_tag="M5T08_RS27420"
                     /old_locus_tag="M5T08_27420"
     CDS             9317..9616
                     /locus_tag="M5T08_RS27420"
                     /old_locus_tag="M5T08_27420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_021515246.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHILYDVDDQARKVRVLAVVGQRQNPHTVS"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(9678..10055)
                     /locus_tag="M5T08_RS27425"
                     /old_locus_tag="M5T08_27425"
     CDS             complement(9678..10055)
                     /locus_tag="M5T08_RS27425"
                     /old_locus_tag="M5T08_27425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021515245.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQGETQERDGLRLPGETEASVSEALISEIKALREEVAGLRQEMQAMRLLEH
                     KPETADTHPETSRRWWHWGKR"
                     /name="hypothetical protein"
     BGC             424..10123
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          4240..4820
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.828"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(749..1476)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.473"
                     /coverage="100.0"
                     /evalue="7.29e-144"
ORIGIN
        1 atgacgaagc aggaaaaaac tgccctcaat atggctaaat tcattaagga ccagtcgctg
       61 ctgttgctcg agaaactgaa cgagctggat atggataccc cggccgacat gtgcgagcgg
      121 cttcacgagg atgcggaaac actctggcag gtgatgcggg ataagctcgg gaaggaataa
      181 cagcaccggc catgccggtg ctgccgttta ttttttgccg gacggccacc accggcgggc
      241 gggtttgtcc tccggcagtt caggcaccgg cacgttaaaa tagcggctgt tatattcccg
      301 catcagctcc gctttcagct tctcgttcac cgcgctttcc ccgtgccgct cgcttaccac
      361 ctcaatcatc gcgttaatct gctccggtga gcacttcccg gcatcaacca cctgcacacc
      421 gttctcgatg gcgaaccgct ccaccttgcg atagaggtaa tcactcagct tcattcctgt
      481 gggtttgtcc cgttcccatt gcggattttc accggtgaaa aagtacgtgg ctttttcgtc
      541 gctgttgagc cgctcgtcat acatcccgat ggatttcatc atcgccagat gctgggcccg
      601 gtcgacaaaa cagaaaacat acgccccggt ctgccaggcg agcaccacca tatcttcaag
      661 atgctcactg gtcggcatga tgcagcgctc gccgtcccgg tcctccgcgt cttcaataaa
      721 taccatgtac agcgccataa tccctcactc cgttgtcata aatttaaagc acgcaaacag
      781 ccgttcacga tccagctccg ccgttttgcg ggcctcgccg gtctgtggat tcacgatttc
      841 atccaccgac aggatcgccc gctgcaccct gacctgcgtc gcccggtccg ggcagatgta
      901 ccagatttca tcgatgccgt tcgcccggcg gttaaacaga tacccggaca ccacctcctg
      961 atagcgcctg cggctcttca cggtgcgctc cacctcaaaa gccaccgttc tgccgtccag
     1021 ctccgccagc gcgtccggca ccttcatccc tttcagggca atacggtgca ggtaccgcca
     1081 gccggtggcc ccccgggcct ccatggcgag gcgcagggac tgcacggcca gccggtgcgg
     1141 cagctgcgcc gccgacaccc gcgacggctg aaacggaata atgtccgtca gcggctcgtc
     1201 ctcgtcgaac gagaacgcca ccccgtgcgg cgtcagcccc cacacgctga cctgacggcc
     1261 gtcaaccggg tatttcgccc ggaccagcag ccctttcgcc accaggctgt ccaggaagcg
     1321 gtaaaccggc tgtaccgccg caatccccag ccgtcgcgca accagctccg ccgtggaaca
     1381 cagctcttcc cgcagccagc gcagggtcag ccgtgttttc tccgcgttcc gcgcctgccg
     1441 cttcgcgtgg ctgacgttgt atcgctccag tgccatcagt acagccccgc aaaatcatca
     1501 tcacccggcc gctgccgcct ggcctccggc tggtactccg ccagcgggtc cgttttcgcc
     1561 agcaccggga aggttttcag cggaatttcc cggcgatcaa cccgcagcgg gctgctgaac
     1621 gccagccgcg ccacgcccag cccggtcatc accgcgaacc cgtcaggcag atgttgcagg
     1681 gtgttcacgt caaacagcgg ccgcgcctcc ttctggatga agctgtcccc gctgatatgc
     1741 tccacgttcc ccgcctcccg gctggcccgg cggcgctcca cgtccacccg gatttccccg
     1801 gtctggcccg ccgcccactg cgcggactcc tggctggccg cccggtagaa ccagcggatc
     1861 ggcgtgttat ccagcaccgt ggttttcaca aaatcagcgg gcagatcctg gccgcactgc
     1921 ccgaagtcgc ccagcgactg gtgcgcgagc agcaggttgc agtcccggct gcgcagcgtc
     1981 cccagcgcgt tcagcacgta cttcgacagc aggtatttaa tctcatcgag catgatgctc
     2041 gcgtgcggcc acctccggaa ctcatcccgc gcaatgatga tttgtgcgca gcgggcaaac
     2101 agcatcttct gcacccggat caccgcctca tcgtccatcg agccgatgac gtacaggcag
     2161 ccgccgccgt tgattatccc cgccacgtcg atgccgctgt ccgtctgcag gacgctcagg
     2221 tcggccacgt tctccagctt ggtgatcagc cccttcacgt cctttttcag ggcctccggc
     2281 agcgcgtacg ccgccgccag cacctgctga atgtttgccc cctgcggaaa ctgctccgca
     2341 atcagcttcg ccgctttctg ctcgctgatg cggtagtggt ccgccacgtc gccggtctca
     2401 ctcaggttaa acccggcaac cagcagattc ttcagggcgt actggtcgag atcgcggaac
     2461 agattcagct gcgccggttt ccccggccgc agatccagca gggtgaagtt tgggcagtgc
     2521 gcccggaaga cgtgcggcgc ccacgcgtcc cctttggggt cgaagtacac caccgcgtcc
     2581 ccgaaatcgc gtacgcagcg aatgagcgcg ttggtggcca tcacgctctt gccgctcccg
     2641 ggcaggccga ggatctgaat gttggtcttg cgccaggtat cacgcgggac cagcaccggc
     2701 tggccctgct catccttacc cagaaacacc gcctgctgtt tccgggcctg ctccgtggaa
     2761 aaatcggcca gcgcgttgac cggtaccggc tccccgaaat gcaggtggcg gacgtcggtc
     2821 atttcaccgg tccgctggct gctgcgccgg ctctcctggt tcagtttcgc gttcaccgcc
     2881 tcgtcagcag ccgcaaaatg accgtcaagc agcaggaacg ccagcagccg cgccaacaca
     2941 atcccgacaa ccagcccgcc cgcgcaccag gcaatcccga acaggttagc cggctgcagc
     3001 aggtaataat ctgcgttcgc gtagaggtcc ggacgcagga cggtttctcc ccagctctcg
     3061 ataaacacgg cgacaaccag cccccccaga agggccagac cggcagcgcc ggtcagccag
     3121 tacgcgtagt tccagacatg ccgccgtgcc ctgaccatcc gcccgggcga atccagcgag
     3181 gccagcaccg aaaatggcgc agtccggtta ttatcgcgct tatagcgatc ggcatactcc
     3241 cgtcgctcct tatgactcag ccccacaaaa cgcatccgtc cggcccagag ttcagccaga
     3301 ccgaaaaaca ggccgataac caggaaagcc ccggccagac cgtaatactc cctgtaaagc
     3361 cagctgaccc acgggtaaat ctgccgggcc agctccggga tatgccactg ccaggcaagc
     3421 gaatccgtat taaacatgta atctctctcc ttcagacgac gggtttaccg gcgtggtgtg
     3481 cagcccccgc tgcacaagcc gcggacatgc cccaagggat ggcggcggcg ttcacgtccg
     3541 tggtgactgc gttcagcttt caccacggag gccagcctct ttccgcccca tgcggaaaag
     3601 gcgcagcccc ggaggaacaa cgagcacgcc ccagcgtgtg agaggaaaag gcggcacccc
     3661 agtgcggtct tttccggtgt tccggccccc gtcagagttc gccatagcga gcggctgacc
     3721 acccgcaaaa tcagaggcag ctttgctgcg tcgtcaggtg cgtccagcac cgtggatttt
     3781 tgtgggtggt cgggcgtgag cctgacgggg gccgggtgaa tttctggcgt ggccggcgtc
     3841 cagccggaca gagccagaaa gagggggaaa gaccgtgcgt cggtggcgtc cagccagcgt
     3901 aagcagggga tttccccctg aagtcagcca cgccacagcg gggatgactt gcatttctcc
     3961 gcaaggagaa aaaagcaaca gagataggtt aacgtaaaat gttcagcgaa aaaccgacat
     4021 ggttcaccta tcctgataat ttatcgtcag ggaatagaaa gacgtaatca ggggacaata
     4081 tcccacatca gcgcgtccgg tgaccgtctt gcaggtgccg gggaaattgc gcctcctcgc
     4141 tcactcggtc gctacgctcc gggcgtgaga ctgcggcggg cgctgcggac acatacaaag
     4201 ttacccacag attccgtgga taagcagggg actaacatgt gaggcaaaac agcagggccg
     4261 cgccggtggc gtttttccat aggctccgcc ctcctgccag agttcacata aacagacgct
     4321 tttccggtgc atctgtggga gccgtgaggc tcaaccatga atctgacagt acgggcgaaa
     4381 cccgacagga cttaaagatc cccaccgttt ccggcgggtc gctccctctt gcgctctcct
     4441 gttccgaccc tgccgtttac cggatacctg taccgccttt ctcccttacg ggaagtgtgg
     4501 cgctttctca tagctcacac actggtatct cggctcggtg taggtcgttc gctccaagct
     4561 gggctgtaag caagaactcc ccgttcagcc cgactgctgc gccttatccg gtaactgttc
     4621 acttgagtcc aacccggaaa agcacggtaa aacgccactg gcagcagcca ttggtaactg
     4681 ggagttcgca gaggatttgt ttagctaaac acgcggttgc tcttgaagtg tgcgccaaag
     4741 tccggctaca ctggaaggac agatttggtt gctgtgctct gcgaaagcca gttaccacgg
     4801 ttaagcagtt ccccaactga cttaaccttc gatcaaacca cctccccagg tggttttttc
     4861 gtttacaggg caaaagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     4921 ttctactgaa ccgcgatccc cgtcagttca gaggaggacg atggtgccac cgttccaccc
     4981 tgaacatccc ggatatagtt tgcctgacat gcgaccagaa gaaagcctga aactatcaat
     5041 ataaaaagaa aaatagcctt tgcctttttc atataccttc cttgtattca gaggcttcgg
     5101 cattcatccg tgaacaccgg aagcctgtat ttcaactcat ccttgataac ttttgcttac
     5161 cagttaccat cccggtaaac aaaggataca aagtaatcaa atttactaat atcgattaca
     5221 tttttaaagt gtggctgtaa tatcgaaacc catggttttt caacatcaaa ccaaccatta
     5281 ttaatattat cctttaaagg catccccaga ctttcaatga cagaaccatc atcaccgaag
     5341 tcttttgagt attcaccgcc tttaaactct tcggttttct tatcaaacca atgaatatgt
     5401 aatttaagcc ccatatttac ctcttaaaga tattttttaa tgtttcgttt tggatccggc
     5461 cctttaacct gcttacccgt ttttggatcg aatgcgccga ggtgttcgcc atcactggcg
     5521 cggtatcctt caagctcacc gtgctgggaa tcccattcat aaattttacg ctttttatca
     5581 ccataccatc gggcccgctt accacctcct ccttgtttag gggttttagg ttttccctct
     5641 ttcaactctc ccaacccctt aatatcttca gtcttgggat caggaaaata atcatggcca
     5701 taatctttag ttcccttgcg aggctttttc ttttcctcat ttaatttatt ttctgcctcc
     5761 ttcgcttttt tctccttttg tttccggctc tccatcgccc cggaaagcgc agccttatct
     5821 ctggataagg catcattcat tgcgtttaaa gatgcctgag cagcttttaa tttcgcattc
     5881 tgattctgaa tatccttatc aagatagcga acctgatcct gccacccgga gccatattca
     5941 tgaggagggt tataagtcac aaaatcatcc ttattcgcct ccgcctcttt caccttttta
     6001 tcagcttcct ggacagcagc cctggcgcca ggaatcgctt tttctgtcga tgtaatcctg
     6061 ccgtttaaag tcgtaatatt tttatcttcg gcatccagct cagctttcgc tttatcatat
     6121 tctcttttca gcccctcttc cggatgagcg gcgtcccatg cctgctggcg acgcttttct
     6181 tcctcctgac gctgttttac ctgtgccggg gtaagaacat ctgtcaccga cacatagacc
     6241 ggcttctgtc cggtctcttt cgggaaacga ataacggcct cacgggagtt accaccggcc
     6301 gtaaaaccag ccggacgtga atcacctttt tcagcaacaa tgccttttgg cggggctttc
     6361 gctgccggaa cacctttcgg tacgctcacc tgcagagccg ggagacccgg aatagacaca
     6421 ctgaataccc ccggacgttt tgtcggtttc gcatccacca caggaacact cattggccga
     6481 cccgcgacaa ccgcaatatg ctgccgctca tccttcacca catccacaac ccgttgtctg
     6541 acacgaaccg tcgcttggtc cagcggtaaa gtacttgccg gagtctccgt caccgtatcg
     6601 gccggtaatg acgtcataat ttttgacatc attttcggat catcttttgc aatctcagaa
     6661 ggcagtacac catagatcgc aatcccccac agaccaaact taaacggacc tttcagggca
     6721 gccagcacat cggcaacggc tgacgacagc gcatcgccgg aaaaggataa agccagtcct
     6781 tcagcccccg gtgtagccag ggcaggcaga ccgaaggcca tcgcggtggc cgaagactgt
     6841 ccaccaccag agctaccgct atgattgccg ctgttaccac cattaccacc gggtttactc
     6901 ccgccgttac caaactcccc cggattatag ttatgaatat ctccattcgg cgtatgcgtc
     6961 gtaccccagc ccggcccgct gtttggatcc gtaccaccac ctgatgaagg gccaccttta
     7021 ccagacgtac cgttcacatt cccactggcc tgaccaccat tatgtcccag acctgaatta
     7081 cccggacctc gaccgtctcc accactcata tcgttttcct ctttgacatt taaaacaata
     7141 aattaaaacc aaaatactgt atatatacac agtatgatta ttaccttact cctccagttt
     7201 tcaggtgtca atatatgaga ttagttctca tcttcaggca caaaaaagcg cccggagccg
     7261 cctgtaagca gatcgcgtct tttcccgaaa atttctcatg gcaacaaatt ttattatttg
     7321 agatattcat cacacttact tttatattgg tatagatgta cctgaaagcg aaatggcatg
     7381 aggagaggac ctgcggttaa actgttcggg aaacctgaga cgcccggagg ccactatgac
     7441 gcacctgaaa ctgactcccg ctgaagaaaa gcgcctggcg gacgcctttg tcgctcaggc
     7501 actggccatg ggcctgaaag ggaacaaaac gctgccaaag gcggtaaaac aacaggtgcg
     7561 ggaaagggtg cttcgtgcca ttcaggacga acgtgaagcc aggcgggcgc aggaaatgcg
     7621 tcgggagaaa tacgaggcgg cggaaaccac cttcacctgg cagccgcagc gccgccggtg
     7681 aaaacgattt aaggttctac tttcaggtgt atccacttcg gaagcatttt agggatatgg
     7741 tattatcgaa aatcatgctt tcggcaatcc aatgggttta tacgatttaa aaagggaaaa
     7801 acatggacaa aaggacaacg ttagtagctc tggtttctat catctttttc acttctggat
     7861 gtgatgacca gaaaaaaggg aatggcaacg ctgaatcgac acaaatcact aaaaacgata
     7921 taacaactca acttaagtta gataaaacgc aacaacctga cgatatagaa aagaaaataa
     7981 aagaaatcca ggccaactcc acccctgaaa aaataaaaaa attcaatgaa ttaaaagatg
     8041 acgaccctaa aatcctacga gagtcggttg tttcagaaat taagaaaaaa ctaccactat
     8101 tggttgacga agccacatta atgacagatg tatcaactga cggaggtaca ttctcataca
     8161 aatatgtaac caaagggatt tcagccagca cgatggaaag tgatgtgtgg aaagatgcca
     8221 tgcaaaaaaa cataaaaaat agctactgct ctgacgatgc cagactaaaa gtattcagag
     8281 agctatttcc tgaaggtgtc atttacaact actacttaag tgacaaatta atctacacat
     8341 acaaggcact tccatcgatt tgttcctaaa gtatttacca taacgtcgca agctgggcgc
     8401 cccagcgggc ggcttttcta tatctgtcct ataaattcag aaaaggaaag ctaaatggct
     8461 gcatatacca gcgaaacagc gaagttttta caaagcgtca gtagcgatca aacatggtgg
     8521 aagtcacgat ataatccagg agacagcgtt ccccattctg gcatttatcg atgtacagtc
     8581 tgcggaaaag agatcaccag taacggcaac gacccgttcc cgccgcaaaa ccaccatcag
     8641 cacagtcagc aacaagcaat caaatggcaa ctgattgtca gaacggacac taaaggtgac
     8701 cgtttcggaa tcaaatgatt gagagccatc cggagctttt tttgtacctg aaactcgaag
     8761 atatttacca taacatccct tatcggtacc gccagtaagg cactggcggc gtttccggcg
     8821 gctacgggat aaaacaagca acaacccacc cggaaaaccg ccggaaacgc gcccggtaaa
     8881 ttttatccta atgcatgcct atgcacgcaa ctgcatagca tatgttttga cgtaaaatgg
     8941 atgccgtaag cccttttttt accgactttc tgcttcatca gcctgactga cacgaccata
     9001 ttctttcaga ccgccccctg ctaaacttct tcatatcacc gcattaaccc gggaggctcc
     9061 gcaatgagca acactacaca ctacgaaaac gccaacttcc tgcgggaact ggctgaaaac
     9121 ctgccccgca tactgccgga aggtggcccg gacaaagctg cgctgttgca acgcctggct
     9181 aacgaagaac tggcccaggc agagtatgaa gatcaggttc gcgctaaagt gaccgccgcc
     9241 cgcgctgaca cgcgctctgg catgaccacg gaacaacttc gccagagact gcatggacgc
     9301 tatcaggaac tacgcgatgc cgtatgaggt ggaatggaag cagggagcca tcgaggatgt
     9361 gacagcattg tttgactaca ttgcggaaaa ttcatccctg tgggatgcgc agaacgttac
     9421 cgatcgcgtt ctcgctgcgg cggacaaact ggccgacttc ccgcgcctct atgaagtgga
     9481 ctcacgctat ggcgaggacg ttcgccggat cagcctgatg ggacagcata tcctctatga
     9541 tgttgatgac caggcccgga aagtccgggt tctggctgtg gtcggccagc gccagaatcc
     9601 ccataccgtt agctaataac cgatgacggg ggcgcaattc attgcgtggc cgtcgccggt
     9661 tggacaagcg cagcgcgtca gcgttttccc cagtgccacc accggcgcga cgtttcggga
     9721 tgtgtatctg ctgtctccgg tttgtgttcc agcagtctca tggcctgcat ttcctgccgc
     9781 agtccggcta cctcttcccg cagcgcttta atctccgaaa tcagtgcctc agacaccgat
     9841 gcttcagtct cacccggcag tctcaacccg tcccgctcct gagtctcacc ctgtcttaat
     9901 tcaccgtaca cccggataag ctcgctggta tccaccaccc gaccaccatc agcttccgta
     9961 cggtatgata cgcggccttt cgccatatca cgatacagag aactccgggc tttccccgtc
    10021 agttccgtcg cctgtctcac cgtcacccac gccattgtct cacctccggt tttgagacag
    10081 tatacccgac aggaagcaca ccacacccgg aaggacagaa aac
//
