LOCUS       NZ_CP103617             5251 bp    DNA     circular CON 20-SEP-2023
DEFINITION  Klebsiella pneumoniae strain 3571 plasmid pMB6975_3, complete
            sequence.
ACCESSION   NZ_CP103617
VERSION     NZ_CP103617.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28174036
            Assembly: GCF_024917495.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 168x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/20/2023 05:38:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,417
            CDSs (total)                      :: 5,297
            Genes (coding)                    :: 5,171
            CDSs (with protein)               :: 5,171
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 48 of 126
            Pseudo Genes (incomplete)         :: 76 of 126
            Pseudo Genes (internal stop)      :: 15 of 126
            Pseudo Genes (multiple problems)  :: 12 of 126
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103617.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="3571"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pMB6975_3"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2018-10-10"
                     /collected_by="MDACC"
                     /genotype="ST3293"
     gene            1..321
                     /locus_tag="M5T29_RS27000"
                     /old_locus_tag="M5T29_27000"
     CDS             1..321
                     /locus_tag="M5T29_RS27000"
                     /old_locus_tag="M5T29_27000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_014386475.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGDD
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            530..>961
                     /locus_tag="M5T29_RS27005"
                     /old_locus_tag="M5T29_27005"
                     /pseudo=""
     CDS             530..>961
                     /locus_tag="M5T29_RS27005"
                     /old_locus_tag="M5T29_27005"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            999..1484
                     /locus_tag="M5T29_RS27010"
                     /old_locus_tag="M5T29_27010"
     CDS             999..1484
                     /locus_tag="M5T29_RS27010"
                     /old_locus_tag="M5T29_27010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_142485265.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEHSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            1488..1700
                     /locus_tag="M5T29_RS27015"
                     /old_locus_tag="M5T29_27015"
     CDS             1488..1700
                     /locus_tag="M5T29_RS27015"
                     /old_locus_tag="M5T29_27015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2333..3688
                     /locus_tag="M5T29_RS27020"
                     /old_locus_tag="M5T29_27020"
     CDS             2333..3688
                     /locus_tag="M5T29_RS27020"
                     /old_locus_tag="M5T29_27020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498067.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_014386473.1"
                     /translation="MFVYLTIENFRSVKEKSTLNLSASGSSNHLTTHIYKNDGMNVGTL
                     MSAGIYGANASGKSNVLMAFEAIKFIATESGDLKEGKRIPCYEPYALSSATNEAPVKFE
                     AEFYSVEGNRFSYEVAFIKNRILFESLDYYPSRVKANLFTRDESDTWETIKFGGHYKGG
                     IKKIPFFPNNSYLAKAGNNAASPDIIKEAYNFFRKGIRHIGLNEKIRISSFGEREDVVN
                     ETAKVLCMVDTGVSGISVREVEAEIPIKMDEEIPQEIKEIIEEDYKYRYMFSHPTDEGG
                     SVSFPMGRESEGTQKLFEIIPLIRSAFNDSMVVIVDELDNSLHPHIADLIVKLFNDPDV
                     NKKGSQLVFSTHNMQLMAPEKMRRDQIWFCEKNKGASSLYSLDDFDKKKIKTTTPYAAW
                     YDEGRFGGVPDINYLKVASFISGDISLVMPDIDVKELSDGFFEEFDGDLSDE"
                     /name="ATP-binding protein"
     gene            3681..4301
                     /locus_tag="M5T29_RS27025"
                     /old_locus_tag="M5T29_27025"
     CDS             3681..4301
                     /locus_tag="M5T29_RS27025"
                     /old_locus_tag="M5T29_27025"
                     /inference="COORDINATES: protein motif:HMM:NF025084.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="WP_023312615.1"
                     /translation="MSKKHGNNKKVPQVMYIYCEGEKTEPLYLEAYISDRSKRTLSVFK
                     IPKTRKNTPEQLVDEAIKKKNSSSSAEGDVFWVVYDQEHLTPSSISCHQKAWNKANRHG
                     INIAISCVCFELWLLLHFGFTTKDFSSYDNLMSSSPLKKLITNYDKGSKLTYLAIRERI
                     GTAKDNARKLDIYAKTNYKNTHEPYLFPCYTSFHELLKAIDDF"
                     /name="RloB family protein"
     gene            complement(4454..4615)
                     /locus_tag="M5T29_RS27110"
     CDS             complement(4454..4615)
                     /locus_tag="M5T29_RS27110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023144844.1"
                     /translation="MHEPPVQSDRCAFSGNYHLESNPERHGSSPLAAAIGNGLEEVSLE
                     VLRQLRLN"
                     /name="hypothetical protein"
     Typing          4966..5138
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.659"
                     /coverage="100.0"
                     /evalue="9.74e-41"
     Typing          complement(4503..5019)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.269"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          312..1816
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.016"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgctcacga tgtgggtgac ggaagacgaa caccggcggc tgctcgagcg gtgcgacggc
       61 aagcagctgg cggcgtggat gcgtcagacc tgcctcgatg agaagccggc gcgtgccggc
      121 agactgccgt cgatatcgcc ggcgctgctg cgccagcttg ccggtatggg gaacaacctg
      181 aaccagatcg cccggaaagt gaatgccggt ggtgccggtc atgaccgggt gcagattgtg
      241 gccgcactga tggcaatcga cgcgggactg gagcggctgc ggcatgcggt gctggaaaaa
      301 gggggggatg atgatcgtta aatttcaccc ccgggggcgc ggtggtggtg ccggtccggt
      361 ggattacctg ctgggaaaag accggcagcg cgagggggcg accgtgctgc agggaaaacc
      421 ggaggaagtc cgggagctta tcgatgcctc gccgtatgcg aaaaagtaca cctccggcgt
      481 gctgtcgttc gcggaggctg aactgccgcc cgggcagcgg gagcagatca tggccagctt
      541 tgagcgggtt ctgatgcccg ggctcgataa agaccagtac agcattctgt gggtggagca
      601 caccgacaag gggcggctgg agctgaattt cctgatcccc aacactgaac tgctgaccgg
      661 gaaacgcctg cagccgtact acgatcgcgc cgaccggccg cgcatcgatg cctggcagac
      721 cgtggtgaac ggcaggctgg ggctgcatga tccaaacgcc ccggagaacc gacggttact
      781 ggtcacgccg tccgcactgc cggaaacgaa gctggaagcc gctcaggcga ttacacgtgg
      841 gttgctggcc ttagcctcat ccggggagct caaaacgcgc gaggacgtca caggggcgct
      901 cacagcggca ggttttgagg tggtccgcac cacaaaaaac agtatcagca ttgccgaccc
      961 ggacgggggg cgaaacatcc gactgaaggg agccatttat gagcagtctt ttagcgctgg
     1021 cgaaggactt ggagcagcaa tcgaaagcgc agcagcagag taccggcgag atgctgaaag
     1081 ccgcattcag cgagcacgag cattctgtaa aagcggaact gagcgcaagc gcgaagagaa
     1141 tcagcgacgc catcagcgcc cacgagcagg gtatgaccgc ggcgatgcag tcgaaccggc
     1201 tgagcgtgct gcgtatggtc ggccggacgt ggctgaccat caccctggtc tcggtactgc
     1261 tgatcgccac gagcgggagc attctgtggt ggcaggggca gcagatcacc gggaattacc
     1321 agactatccg ggcgcaggag cggacgcagg ccatgctgtc ggagaagaac cacggcgtgc
     1381 agctgtcgct ctgcggggag aagaaactgt cctgcgtcaa agtgaacccg aaagcgggag
     1441 cttacgggga ggaagggaac tggatggtac tggagaggaa atagcccatg acggaactgg
     1501 aaaaacagtt gctgaccgca ttagagcagc tacatcagga ctactcgcaa aggctggacg
     1561 agtgggagag cgccttcgcg gaatggcaga agatgtctgg tcttatgcaa cgggagaacg
     1621 cggcgctgaa cgagcgcgtc acgcgcttga gtcagcaggt ggcgaacttg agcaggcagt
     1681 tacagcgttt gagccagtag tgcagcggca tgaaatggcg gtggccgccg aacgggcata
     1741 cgagcagaaa cagcatgaaa aagagctggc ggaaacccgt tcccggcagc ggagctatga
     1801 cggtccgacg ctgggctgac tgcgcgcatc cagtgacaaa ccccaagaag aagggctcat
     1861 aaatttcccg ctaaatacgg tcgtgatgtt tccatcacag cgtatacaac ggagggatta
     1921 tgagactgtt taacgtgatt gagacggtgc tgaattgcct gtacatggcg ctggagatct
     1981 gggactggct ggagaagcat tttcctgttt ttaaggcgtt catccggatt ctggcctgaa
     2041 atcagaccgt atgcatctgg ctgcatgacc atgcagccgg gtaaaattta tctgtcacga
     2101 acggggcggt gtatcgggtg gtttgttgcc ggttttacct gactgccgcc ccgtttgtgg
     2161 cgaacctgtc cggagcggtg cgggcaacag atgttatggt aaatagcgtg taattataaa
     2221 tagcacaacg ttttttaagg aagatgttgt tccaaaatta aaacatatag gatatctttc
     2281 ctatgattga tttttcgctt ttttggcgaa aaatccccgt taggaggtat gcatgtttgt
     2341 ttatttgact attgaaaatt ttaggtcggt taaggaaaaa tcgacattaa acttgagcgc
     2401 atccggttct agtaaccatt taacaactca tatctataaa aatgatggaa tgaatgttgg
     2461 aacgctcatg agtgctggga tttatggtgc taatgcgtct ggtaaatcta acgttctcat
     2521 ggcttttgaa gctataaaat ttatcgcaac agaaagcggt gatttgaaag aaggtaaacg
     2581 gattccctgc tatgagccat atgctctttc ttcggctacg aatgaggctc ctgtaaagtt
     2641 tgaggctgaa ttttattctg ttgaggggaa tcggttcagt tatgaagttg cttttattaa
     2701 aaatcggatt ttatttgaat cattagacta ttatccatct agggttaaag caaatttatt
     2761 tactagagat gagagcgaca cctgggaaac aatcaagttt ggtgggcatt ataaaggagg
     2821 tattaaaaaa ataccattct tccctaataa ttcatactta gcgaaagcag gtaataatgc
     2881 cgcatcacca gacataatta aagaagctta taactttttt agaaagggaa ttagacatat
     2941 aggtttaaat gaaaagataa gaatatcttc ttttggggag agggaggacg tcgtaaatga
     3001 aacggccaag gttttatgca tggttgacac tggtgtttcc ggtatatctg tacgggaggt
     3061 tgaggcggaa ataccaataa aaatggatga ggaaatccct caagaaataa aggagataat
     3121 tgaagaagat tataaatatc gttatatgtt ctctcatcct actgatgaag gggggagtgt
     3181 ttctttccct atggggagag agtctgaagg cacccaaaaa ttatttgaaa tcatacctct
     3241 aattagatct gcgtttaatg atagtatggt tgtaattgtt gatgagttag ataatagctt
     3301 gcacccgcat attgctgact tgattgttaa gttatttaat gatcctgatg tcaataaaaa
     3361 aggctcacaa cttgtattct caacccataa tatgcagtta atggctccag agaaaatgag
     3421 aagagatcag atatggtttt gtgaaaaaaa taaaggtgcg tcgtctcttt atagcttaga
     3481 tgattttgat aagaaaaaga taaaaacaac aacaccttac gcagcttggt atgatgaggg
     3541 taggtttggt ggggttcccg acataaacta cttaaaggta gcctcattta tatctggtga
     3601 tatatccttg gtaatgccgg atattgacgt caaagaattg tctgatggtt tttttgaaga
     3661 gtttgacggg gatctttctg atgagtaaaa aacatggaaa taataagaaa gtcccacaag
     3721 taatgtatat atactgtgag ggtgagaaaa ccgaaccatt ataccttgag gcttatattt
     3781 cagacagatc taaaagaact ttaagtgtat ttaaaatacc taaaacaaga aaaaatacac
     3841 ctgagcagtt ggtggatgag gcgataaaga aaaagaattc atcatcttcc gcagaaggtg
     3901 acgtcttttg ggttgtttat gatcaagagc atctgacgcc ttcgtctatt tcatgtcatc
     3961 aaaaggcatg gaataaagct aataggcatg ggataaatat agcaatatcc tgtgtttgtt
     4021 ttgaattatg gttattgttg catttcggct tcacaaccaa agatttttcg tcgtatgata
     4081 atttgatgtc tagctccccg ctaaaaaaat taataactaa ctatgataaa ggtagtaaac
     4141 taacttacct tgctataaga gagaggattg gtactgcgaa agataacgca agaaagctag
     4201 atatttacgc taaaactaat tataaaaaca cgcacgaacc ttatctattt ccatgttata
     4261 catcattcca tgagctactt aaggcgattg acgacttttg aagtaatcca ttgaccatgg
     4321 cttattacag gctgtaatct atgaaaaata aaaaccaccg ctaccaacgg tggtttttgg
     4381 aaggtacatg agctaccaac ttatgtaccg ggtaactggc ttagagggag cgcagtcacc
     4441 aaaaattgtc ctttcagttt agccttaact ggcgcagaac ttcaagactc acttcctcta
     4501 atccgttacc aatggctgct gccagtggcg atgaaccgtg tctttccggg ttggactcaa
     4561 gatgatagtt accggagaag gcgcagcggt cggactgaac ggggggttcg tgcatacagt
     4621 ccagcttgga gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacaaacgc
     4681 ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg
     4741 gagccaccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccaccactga
     4801 tttgagcgtc agatttcgtg atgcttgtca ggggggcgga gcctatggaa aaacggcagc
     4861 ggcgcatcct ttttctgccc cgtttgtcat gccatatgcc ttcgctcttc ttcgtgtgca
     4921 ggtaacgccc gccgcagccg aacggcagga gcggcagcga ctgagtgagc gaggaggcgt
     4981 catatcctct gaacttccat tttctgcctt acgcccgcag ctgcattttc ctctgtcaca
     5041 tgaagcactt tcctgattac cgcactcatg ccaacataat aagccagtat acactccgct
     5101 agcgctacgt gactggttca gggctgcgcc ccgaaacccg ctaaaagcga ctgacgcgcc
     5161 tgcggcttgt ccaacaccgt gccgaccggg aaacatttcc cgcccgtccc ggtgttatca
     5221 ggaggctact ctggcagaca aacgcaacaa g
//
