LOCUS       NZ_CP103616             5883 bp    DNA     circular CON 20-SEP-2023
DEFINITION  Klebsiella pneumoniae strain 3571 plasmid pMB6975_2, complete
            sequence.
ACCESSION   NZ_CP103616
VERSION     NZ_CP103616.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28174036
            Assembly: GCF_024917495.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 5883)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 168x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/20/2023 05:38:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,417
            CDSs (total)                      :: 5,297
            Genes (coding)                    :: 5,171
            CDSs (with protein)               :: 5,171
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 48 of 126
            Pseudo Genes (incomplete)         :: 76 of 126
            Pseudo Genes (internal stop)      :: 15 of 126
            Pseudo Genes (multiple problems)  :: 12 of 126
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103616.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="3571"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pMB6975_2"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2018-10-10"
                     /collected_by="MDACC"
                     /genotype="ST3293"
     gene            join(5568..5883,1..11)
                     /gene="mobC"
                     /locus_tag="M5T29_RS26955"
                     /old_locus_tag="M5T29_26955"
     CDS             join(5568..5883,1..11)
                     /gene="mobC"
                     /locus_tag="M5T29_RS26955"
                     /old_locus_tag="M5T29_26955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_131091209.1"
                     /translation="MEKRTKEIKIRLTETEHQRLLDRCDRTHLAEWLRQLGLGEHTSRK
                     RRIPNVAPELLRQVSGIGNNLNQIARRLNQADSLTPSERVSLLVVLTSLDRQLGDLLEQ
                     NRDR"
                     /name="mobC"
     gene            220..>642
                     /locus_tag="M5T29_RS26960"
                     /old_locus_tag="M5T29_26960"
                     /pseudo=""
     CDS             220..>642
                     /locus_tag="M5T29_RS26960"
                     /old_locus_tag="M5T29_26960"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            689..1174
                     /locus_tag="M5T29_RS26965"
                     /old_locus_tag="M5T29_26965"
     CDS             689..1174
                     /locus_tag="M5T29_RS26965"
                     /old_locus_tag="M5T29_26965"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_131091215.1"
                     /translation="MSEILNLAKGFEEKSKQQAQDTEQSVKREFERLETFISAELQSSS
                     QSISNAIREQNGRLTGMLRTSVSTAMAWIFICFALLIGILGGIIWFQGTIITSRLSEMD
                     SYSQQLASLKAKSGAGVTIYSDDKHRNTYMVVLPKQAHGVETYTSTTDNTVVKYTAK"
                     /name="MbeB family mobilization protein"
     gene            1244..1468
                     /locus_tag="M5T29_RS26970"
                     /old_locus_tag="M5T29_26970"
     CDS             1244..1468
                     /locus_tag="M5T29_RS26970"
                     /old_locus_tag="M5T29_26970"
                     /inference="COORDINATES: protein motif:HMM:NF016769.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_241506735.1"
                     /translation="MEKQLLSALESLQTSYEQQQQAWQASYSSLQHMFEATSQALAHSD
                     RVCQHLSSQVDSLRAQVESLSRNVSRLMR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1630..1962
                     /locus_tag="M5T29_RS26975"
                     /old_locus_tag="M5T29_26975"
     CDS             1630..1962
                     /locus_tag="M5T29_RS26975"
                     /old_locus_tag="M5T29_26975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000017531.1"
                     /translation="MSHAIEFIETPMFTRQIKQIATDEELRELQKTLIESPDKGDLIQK
                     TGGLRKIRMATGNQGKSGSVRVIYFLATAEVIYLVMAYPKSTKDSLTDAEKAALKTLTQ
                     QLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1964..2248
                     /gene="nadS"
                     /locus_tag="M5T29_RS26980"
                     /old_locus_tag="M5T29_26980"
     CDS             1964..2248
                     /gene="nadS"
                     /locus_tag="M5T29_RS26980"
                     /old_locus_tag="M5T29_26980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_001697687.1"
                     /translation="MSFFDELKASLEEAVEIKNGVREPARVTRYELADVKAIRAQLNVS
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFQELAAH"
                     /name="nadS"
     gene            2440..3381
                     /locus_tag="M5T29_RS26985"
                     /old_locus_tag="M5T29_26985"
     CDS             2440..3381
                     /locus_tag="M5T29_RS26985"
                     /old_locus_tag="M5T29_26985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_131091212.1"
                     /translation="MSNAALQLFNERLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGAAIDWSDRNAPAPTLTITNPDNGHAHLLYALKTSVRTAPDGKMK
                     PLRYAAAVENALRKKLEADAGYSGLICKNPNHRHWKIAVWQPELYTLDWLADFLDLNAA
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLQACYERACAYNLQFVAPLD
                     ESEVSGVAKSIAKWTHGKFSKSSFDEYVSMTHTSPIQAARGRKSRGGGRPRVSEEKIKE
                     IIFLRKMNKSIREIADLLNLSVGCVFKHIEGI"
                     /name="replication initiation protein"
     gene            3382..4689
                     /locus_tag="M5T29_RS26990"
                     /old_locus_tag="M5T29_26990"
     CDS             3382..4689
                     /locus_tag="M5T29_RS26990"
                     /old_locus_tag="M5T29_26990"
                     /inference="COORDINATES: protein motif:HMM:NF024573.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_259388001.1"
                     /translation="MRVEIKNVGSISNFNVDISGLTVVSGENDSGKTALSKVIYSLGQS
                     SSFYKFKRKILVRNRIKNIYDKMFMLLNKYIHAEFPDENEEDLNGALTALMGFLYTARR
                     MTSADDLDFDPIYVVENTEHILRYFHISDDEVRKELTHMVLILRRIAMDNVRFADHFLS
                     SLKEEFSSDLVKKNSISEFSEITITVGGERSYVKFDNKKIIETSGDFDIPFIDSTFVDG
                     PAVFLLEKVLGRTDYVMRSKVRSSLPHHVIDLCSKIDGTRNTLSSINEDDETCWNMNDF
                     YDGSISFEPEKEDFHLKKDGYFFSGNNVSSGVKALSVMDILSRGGYLNTESLIILDEPE
                     TNLHPKWQKKYAEAIVKLSQKGVRILVNTHSPYMLESLKAYSGFFKSSAKFYFSHKVRD
                     EVSLIDTHGDISIIIDALSGPLRDLMLEMQGEPDDF"
                     /name="AAA family ATPase"
     gene            4679..5347
                     /locus_tag="M5T29_RS26995"
                     /old_locus_tag="M5T29_26995"
     CDS             4679..5347
                     /locus_tag="M5T29_RS26995"
                     /old_locus_tag="M5T29_26995"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_259388002.1"
                     /translation="MISNEKKLIDFIESQYSCFSGTLSEISKNGDNLFIDMNCNFIHLD
                     KIPMLIESGEVEANCFSVDTIVYCSVKDCLYLIEFKEGWPQGDSAKELRFKCYETISKL
                     IRNWSTHIGHRHDFFKLKIKYVVITRPPKKNIDVGGVINKSFLGVLDASKGFFKLQVLN
                     KTYVDDVRVFVEDEKIFKFLSRITKHNSMNYYHKGQHTRTEWIRDPQSGTVSSSIHTCS
                     "
                     /name="hypothetical protein"
     Typing          2..1566
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.636"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtgatcgtta aaattcattc gcgcggcgcg ggctcaggca gtggcccagt ggattacctg
       61 ctgggaaaag accggcagcg tgaacaggcc agtgtgctgc gcggcaatcc tgaacatgtg
      121 cgcgagctga tcgacggctg tgactttgcc cgcgcctaca cctccggtgt gctgtcattt
      181 caggagccgg acattgcaga cgccgagaaa tcgcgcctga tggatgaatg ggagcatacg
      241 ctgctgaccg gcttcgaccg tgaccagtac gcctgcctgt gggtggagca ccgcgacaag
      301 ggacgacttg agctgaattt tgtcatcccg aacatcgaat tgcagagcgg caaacgcctg
      361 caaccctact ttgaccgcgc cgatcgtccc cgcgtcaacg cctggcagac cctgaccaac
      421 gaccggctgg ggctgcgcga cccgaacgac ccgacttacc gccgcccact gacgcaggcc
      481 agcgatcttc cccgcgacaa acagcaggcg gcagagaaaa tcaccgccgg cctgatgaac
      541 ctgatgcagc agggtgtgat ccgcagccgg caggacgtgg tgacgcagct ggaaagctac
      601 ggcctgaccg tggcgcggga aacgaaaagc agtatcagca ttgccgatcc ggacggcggc
      661 cgaaatatcc ggctgaaagg aatgatttat gagcgagatt ttaaatttgg cgaagggctt
      721 cgaggagaaa tcgaagcagc aggcgcagga taccgagcag agcgtgaagc gcgagttcga
      781 gaggctggag acgtttatca gcgcggaact gcaatcaagc tcgcagagca tcagcaacgc
      841 tatccgcgag cagaacggca ggctgacagg gatgctcaga acctccgtct caacggcgat
      901 ggcgtggata ttcatctgct tcgccctgct gatcggcatc ctgggcggga taatctggtt
      961 tcagggcacg atcatcacct cacgcctgag cgaaatggac agctacagtc agcagctggc
     1021 gagcctgaaa gcgaaaagcg gcgcaggcgt gacgatctac agcgacgaca agcaccggaa
     1081 cacctacatg gtggtgctgc cgaagcaggc gcacggggtg gaaacctata catcgacgac
     1141 cgacaatacc gtggtgaagt acacggcgaa ataaccctgc cagagcgccc tgaaacgtca
     1201 cagcgcgttt ttaacaccga ggataagcaa aaccatgacc gagatggaaa aacagcttct
     1261 gagcgcctta gagagcttac agacaagcta cgagcaacag cagcaggcgt ggcaggccag
     1321 ctacagcagt ttgcagcaca tgttcgaggc tacctcacag gcgctggcgc acagcgaccg
     1381 ggtgtgtcag cacttgagca gtcaggtgga cagcttgcgc gcgcaggtcg agagcttgag
     1441 caggaacgtc agccgcttga tgcgctgatc aggcagcacg accgcgccta cgccgagaag
     1501 ctgcgtgagc agcagccaga gccggagccg cagaaaaaac cgacccgcta ctatggtccc
     1561 tctatgtgac ggcttttccg gaatcagaat taagtgtact atgtactcca ttgaaaacag
     1621 gagacagccg tgagtcacgc gattgagttt atcgagaccc cgatgtttac ccgtcagata
     1681 aaacagatcg ccacggatga agaactcagg gagctgcaaa agacgctgat tgagtcgccg
     1741 gacaaaggcg atctcattca gaaaaccggc gggctgcgca aaatcaggat ggcgacgggc
     1801 aaccagggaa aaagcggcag cgtgagggtg atttactttc tggccaccgc cgaggtgatc
     1861 tatctggtga tggcctaccc gaaaagcacc aaagacagcc tgacggacgc ggaaaaagcc
     1921 gcgctgaaga cgctgaccca acaactgaaa gacgaggtgt gacatgagtt tttttgacga
     1981 gctgaaagca tcgctggaag aagccgttga aatcaaaaac ggcgtcagag agcctgcccg
     2041 cgtcacccgt tacgagctgg cagacgtgaa agccatcagg gcacagctga acgtatctca
     2101 gagcgagatg gcaaaagtgc tgggaaccag cctcgacacc atcaagagct gggaaaccgg
     2161 acgccgtaac ccgaccggac tggcggcgaa ggtactggca accatccagg cgaacccgaa
     2221 attcttccag gaactggcgg cgcactaagc cgttgtgaca gaaggattcg acaggcgagg
     2281 ccgttcgcgt gtaatttaac ggcaacagaa aaaacaaagc cccggaaatc atgtcatatc
     2341 ttggcggatc gcatgataac cagggctagg tcgaaacctt gaaggaatat tagcacatga
     2401 gccgtgccag acaacagccc gccataagcg cagggcgctt tgagtaacgc tgcactccaa
     2461 ctttttaacg aacgtttacc gcacaagccg tacttttcgg acgatctgca ctttggcgta
     2521 cgcattgccg gtaaggaacg cgccattctg gcgaaataca tccagttcaa tcagcctcac
     2581 gccatgttct ggctgggatt cgacgtcgat cgcgccggtg ccgctattga ctggagcgac
     2641 cgcaatgccc ccgcgccgac actgacgatc accaaccccg acaacggaca cgcgcacctg
     2701 ctgtacgcgc tgaaaacctc tgtacgtacc gcgccggacg gaaaaatgaa gccgctgcgc
     2761 tacgccgccg ccgtggagaa tgcgctacgt aaaaaattag aggctgatgc ggggtattcg
     2821 ggactaatct gtaaaaatcc gaaccacagg cactggaaaa ttgccgtgtg gcagccggaa
     2881 ctctacacac tggactggct ggccgatttc cttgacctga acgctgcgaa cgataaagaa
     2941 atcgttgccg actacggatt aggccgtaac tgcaccctgt tcgacaaaac ccgtaagtgg
     3001 gcataccgcg ccatccgtca gggctggcct gagtatgagc agtggctgca agcctgctat
     3061 gagcgtgcct gcgcctataa cctacaattt gtagcgcctc tggatgaaag tgaggttagc
     3121 ggggttgcta aaagtattgc caagtggaca catggaaaat ttagcaaatc atcgtttgat
     3181 gagtatgttt ctatgacgca cacttccccc attcaagcgg ctagaggaag gaagagtcgc
     3241 ggaggtggaa ggcctagagt tagtgaagaa aaaataaagg aaattatttt tctacgaaaa
     3301 atgaataaat cgataagaga aatagccgat ttattaaact tgtcagttgg atgtgttttc
     3361 aagcacatcg agggaatttg aatgagagtt gaaattaaaa atgtaggatc tataagcaac
     3421 tttaatgttg atatttctgg attgacagtt gtttctggtg aaaacgactc agggaaaacg
     3481 gctctctcaa aagttattta ttccctcggt cagagttcat ctttttataa gtttaagcgt
     3541 aagattttgg taaggaacag gataaaaaat atatacgata agatgttcat gcttcttaat
     3601 aagtatatac atgctgagtt tccagatgag aatgaggaag acctaaatgg tgctttgact
     3661 gcacttatgg gatttctcta taccgcaaga cggatgacct ctgctgatga tttggatttc
     3721 gatccaattt acgtagtaga gaatactgaa cacatactgc gttacttcca tatatcagac
     3781 gacgaagtta gaaaagagct aacacatatg gtcttgattc tcagaagaat agctatggat
     3841 aacgtgcgtt ttgctgacca ttttctttca tccctaaagg aagagttttc cagcgatttg
     3901 gttaagaaaa actctattag tgaattttcc gagataacta taactgtggg tggtgaacgc
     3961 agttacgtga aattcgataa taaaaaaata atagaaacca gtggtgattt tgatataccg
     4021 tttattgatt caacatttgt tgatggccct gctgttttct tgctggaaaa ggttctagga
     4081 cgtactgatt atgtaatgcg atctaaggtt aggtctagcc tcccacatca cgtgatagat
     4141 ttgtgctcta aaatagatgg aactagaaac acactctcat caataaatga agatgatgaa
     4201 acatgctgga atatgaacga tttctatgat ggaagcatat catttgaacc agagaaagag
     4261 gacttccacc ttaaaaagga tgggtacttt ttttcaggga ataatgtttc atctggtgtg
     4321 aaagctcttt cggttatgga tattcttagc agaggagggt atttgaacac agaaagccta
     4381 ataatcctgg atgaaccaga aacaaaccta caccctaagt ggcaaaagaa atacgcagaa
     4441 gccatagtga agctttcaca gaagggagtt aggatactcg ttaatacaca tagcccttat
     4501 atgttggaat cacttaaagc atatagtggt ttttttaaat cctcggcaaa gttttacttc
     4561 tcgcataaag tacgagatga agttagtctc attgatacac atggcgatat aagcattatt
     4621 attgatgcgt tgtccggccc tcttcgtgat ttgatgcttg agatgcaggg ggaacccgat
     4681 gatttctaat gagaagaaat taatagattt catcgaaagt cagtactctt gtttttctgg
     4741 gactcttagc gagatctcga agaatggaga taatttattc attgatatga attgtaactt
     4801 cattcatcta gataaaatcc ctatgttaat agagagtgga gaggtagagg ctaattgctt
     4861 ttcagttgac actatagtct attgtagtgt gaaggactgc ctttacttga ttgaattcaa
     4921 agaaggatgg ccacaaggtg actcagctaa agaattgaga ttcaagtgtt atgagactat
     4981 atctaaatta attaggaact ggtctaccca cattggtcat cgccatgatt tttttaaatt
     5041 aaagataaaa tacgttgtta taacaagacc gccaaaaaag aatattgatg tgggcggtgt
     5101 gattaataag tcttttcttg gtgtgcttga tgcctcgaaa ggttttttca aactacaagt
     5161 tttgaataaa acgtacgttg atgatgtaag agtttttgtt gaagacgaaa aaatattcaa
     5221 gttccttagt aggataacta aacataactc aatgaattat taccacaaag gtcagcacac
     5281 tagaacggaa tggataagag acccccagtc cggaactgtc tccagctcaa tccatacgtg
     5341 ttcataaaca acctatatca gataacagcc ctctgctaaa gagggctttt tttttgcagc
     5401 cagcgcagta tctgacatac ttcattgtac cttaagccag tacacacagc gtcccctctg
     5461 atgaggtgcc tccgtgactg gttcaggggg ctcgccgccc cccgaaaccc ctggcatcca
     5521 ctgcgaaaaa ttctcacggt ggtgcgcatt ttctctcagc ggaacctatg gaaaagcgaa
     5581 cgaaagagat caaaatccgg ctcaccgaaa ccgagcatca gcgactgctc gatcgctgcg
     5641 atcggactca ccttgcggag tggttgcgtc aactcggtct gggtgaacac acttcacgca
     5701 agcgccgtat acctaatgtg gcgcccgaac tgctgcgaca ggtcagtggg attggtaaca
     5761 acctgaacca gatcgcccgt cgcctgaatc aggctgattc cctgacacct tctgaacgtg
     5821 tctcgttgct tgtcgtattg accagccttg accgtcagct tggtgaccta ctggagcaga
     5881 acc
//
