LOCUS       NZ_CP103613             5410 bp    DNA     circular CON 20-SEP-2023
DEFINITION  Enterobacter cloacae strain 3143 plasmid pMB6956_2, complete
            sequence.
ACCESSION   NZ_CP103613
VERSION     NZ_CP103613.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28174020
            Assembly: GCF_024917615.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 5410)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 109x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/20/2023 05:47:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,900
            CDSs (total)                      :: 4,787
            Genes (coding)                    :: 4,727
            CDSs (with protein)               :: 4,727
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 29 of 60
            Pseudo Genes (incomplete)         :: 33 of 60
            Pseudo Genes (internal stop)      :: 14 of 60
            Pseudo Genes (multiple problems)  :: 15 of 60
            Pseudo Genes (short protein)      :: 1 of 60
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103613.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="3143"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:550"
                     /plasmid="pMB6956_2"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2018-10-31"
                     /collected_by="MDACC"
                     /genotype="ST995"
     gene            1..324
                     /locus_tag="M5S62_RS24460"
                     /old_locus_tag="M5S62_24460"
     CDS             1..324
                     /locus_tag="M5S62_RS24460"
                     /old_locus_tag="M5S62_24460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000957084.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPARSGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            533..>967
                     /locus_tag="M5S62_RS24465"
                     /old_locus_tag="M5S62_24465"
                     /pseudo=""
     CDS             533..>967
                     /locus_tag="M5S62_RS24465"
                     /old_locus_tag="M5S62_24465"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1002..1493
                     /locus_tag="M5S62_RS24470"
                     /old_locus_tag="M5S62_24470"
     CDS             1002..1493
                     /locus_tag="M5S62_RS24470"
                     /old_locus_tag="M5S62_24470"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000096688.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            1500..1715
                     /locus_tag="M5S62_RS24475"
                     /old_locus_tag="M5S62_24475"
     CDS             1500..1715
                     /locus_tag="M5S62_RS24475"
                     /old_locus_tag="M5S62_24475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_029590204.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     MLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3247..3486)
                     /locus_tag="M5S62_RS24480"
                     /old_locus_tag="M5S62_24480"
     CDS             complement(3247..3486)
                     /locus_tag="M5S62_RS24480"
                     /old_locus_tag="M5S62_24480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609270.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_164525734.1"
                     /translation="MEPFEIPAAELADKLEVSQSALSRLLNGKTDLSYEMALRLSKVIG
                     RSAESWVNMQAQYSLSKSRKTLSETLEHLHPMFP"
                     /name="HigA family addiction module antitoxin"
     gene            3878..4159
                     /locus_tag="M5S62_RS24485"
                     /old_locus_tag="M5S62_24485"
     CDS             3878..4159
                     /locus_tag="M5S62_RS24485"
                     /old_locus_tag="M5S62_24485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000594459.1"
                     /translation="MIKNIKHKGLRSFAENGNTAGIQPHLAKKLRMILTTLSTATCLQD
                     IVDVRALGCHPLKGERKGEFSVEVNGNWRVVFKVEEPNVLDVDLEDYH"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          5125..5298
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.857"
                     /coverage="100.0"
                     /evalue="5.54e-83"
     Typing          complement(4614..5201)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.709"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          315..1807
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.2"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgctcacca tgtgggtgac ggaggacgag caccggcggc tgctcgagcg ctgtgacggc
       61 aggcagctgg cggcgtggat gcggcagatc tgcctggacg aaaagccggc acgttccgga
      121 aagctcccct cactctcgcc ggcgctgctg cgccagcttg ccggcatggg caataacctg
      181 aaccagatag cccgccgtgt taacgccggc ggcggcaccg gccatgacag ggtacagatt
      241 gtggcggcgc tgatggccat cgatgccggc cttgagcggc tgcggcacgc cgttcttgag
      301 aaaggaacgg acgatgatcg ttaaatttca tccgcgcggc cgcggcggtg gcgccgggcc
      361 ggtggactat ctgctgggta aagaccggca gcgtgaaggc gccaccgtcc tgcaggggaa
      421 gccggaagaa gtccgggagc tgattgacgc gtcgccttac gttaagaaat acacctccgg
      481 cgttctgtcg tttgcggagg ccgatctgcc tcccggccag cgggaaaaac tgatggccag
      541 cttcgagcgg gttctgatgc ccggactcga taaagatcag tacagcattc tgtgggtgga
      601 gcacgcggac aaggggcgtc tggagctgaa ctttctgatc cccaataccg agctgctgac
      661 cggcaggcgg ctccagcctt actacgaccg cgccgaccgg ccgcgcatcg atgcctggca
      721 gactatcgtg aacggcagac tggggctgca cgacccgaac gcgccggaaa accgccgggc
      781 gctggtcacg ccgtccgggc tgccgaaaac gaagcaggag gccgcagaag ccattacccg
      841 gggactgctg acccttgctt cgtccgggga gctgaaaagc cgtcaggatg tcactgacac
      901 tcttgaaggt gcaggttttg aggtggtgcg caccacgaaa agcagtatca gcattgccga
      961 cccggacggg gggcgaaaca tccgactcaa gggagccatc tatgagcagt cttttaacgc
     1021 tggcgaagga cttagagcag caatcgaaag cgcagcagca gagtaccggc gagatgctga
     1081 aagccgcatt cagcgagcac gagcagtctg tcagagcgga actgagcgca agcgcgagga
     1141 gaatcagcga cgccatcctc gcccacgagc agagtatgag cgaggcgatg gagaaaaacc
     1201 ggcggagcgt actgcggact gccgggcgga catggctcac catcctgatg gtgtcagcgc
     1261 tgctgatcgg cacgagcggg agcattctgt ggtggcaggg gcagcagata accgacaatt
     1321 acacgcatct tcgtcagcag gaagataccc tggcgaaaat gaccgcccgg acgtggggag
     1381 tgcgctatca ggagagcagc gacggccgga ggtttcttat cctgccgccg gggatgcaaa
     1441 ccgaagccat cccctacgac gggacgacat ggatccgcct gaaacaggag tagcggaaaa
     1501 tgacagagct ggaaaagcag ttgctgagcg cattagagca gctacagcag gactactcgc
     1561 aaaggctgga cgaatgggag agcgccttcg cggaatggcg gagcatgtgt gggcttatgc
     1621 aacgggagaa cgcgatgctg aacgagcgcg tgtcggactt gagcacgcag gtgctgagtt
     1681 taagcgagca gctgcgccgc ttgtccggga actgaatgcc atcgagctcc ggcagaggga
     1741 gaatgagtat cagaaaacac tggagctgga gcgccagccg cagaaaacct accgcgggcc
     1801 gacactttaa accatccagg ctgctgacaa cccgcacgaa gaggggctct tatttctccc
     1861 gctaaatagg ttcgtgatca aaaaggtcac gacttattca ggagcgggaa atgaagctgt
     1921 taaatcttat tgaatcattg cttaactgcc tgtatctggc ggcggagctg tgggactggc
     1981 tggagaagca tttgtcggtt tttaaggcat tggcgcggat tttagtgtga ctttaacatc
     2041 catgcatctg gctgcatgac tatgcatccg tgtaatattc agcagctgcg tttccggtgt
     2101 tttttcgtca tgtctgtcgc cactttctcg cgtctggcac cggaaacgca gccaaccccg
     2161 tttcagcggt gcgggcaacg gatgttatgg taaataagag cgaccgtccc ggtcgctctt
     2221 gaaagagaat tcggccttag gtcagttttc gcagatacaa tcatgtggac tgcggagaca
     2281 ttcttcgcac tcctcacata tgcactcatc cagtttttca ccgcactcgt cgcaacgtga
     2341 gcagctacag tgctcatcta aatttccaca atcgctgcag cgctcacaga cgcagtctgc
     2401 cagcttctcg ttgcagtcat cgcagaagcc acaggtacag gcgctggcag ggagggcaca
     2461 aacatcgcaa atcttatcca ttggatccat tttatctcac ttttatttta tcagtggctg
     2521 tgtgcgggcg atttccccag cgcgatagat gtggatttaa gtgccatttt tcaatgatta
     2581 aggcagctaa aaaagtggtt ttttagggct tttcaacgtg accagcactg acggggataa
     2641 atacaggtaa gcaataatgc tcagtcatga aaaggagaat ccgatggagc aacatacccc
     2701 tgaatactta cgacgtacct tagctcataa ccgtgcccta atggacgata tcattagcag
     2761 tggcatgtcc aggtactaca acacggagat tgtggatgcc gcatgtgagg cgatagaagc
     2821 tgaattgcga cgacgaggaa ttttgtaaga gacaaagccc gcttatgcgg gctttgtcgt
     2881 tactggcgct tctgttactc agtgttgtct ggggttcggt agatctgtag ccagtacctg
     2941 ccaggtgcgc tcgttggtgg tgcgaactca ttatcgcgaa tggtgcagta tcgtccatat
     3001 ttgcgggtaa cagtatgagt gtttgtgttg atgtcacatt cttggaatga gccaccatca
     3061 cgacctattt tgccatgtgg tcctacataa attgccattt tgttctcctt tttagagcct
     3121 tgtatgggca tcttattggt gttcaaacaa caatcatttc aagatctaag aactgaacta
     3181 aaaatcaaac taaaggtagg tatgcggggg ttttacggca aaaagcccgc tcgtgcgggc
     3241 ttttacttat ggaaacatcg gatgaaggtg ttctagcgtc tcggataaag tcttacgact
     3301 tttcgagagg ctgtactgag cctgcatatt aacccacgac tctgcagagc ggcctataac
     3361 tttagatagt cgaagagcca tctcataact aagatcagtt tttccattga gcaatctact
     3421 taatgcagat tgtgatactt caagcttatc agctaactct gcggcaggta tttcgaacgg
     3481 ttccatgaac atttccctga tgaattcacc ggggtgtaca ggatcgtaaa gatttatcat
     3541 gccttcctct attggttttg tatttgatta cacaaaacat tttgtttctt tcgcgttgcg
     3601 cattgcacaa tacgcaagtg tctgttattg acaaaaaaag cagtaagctt taggatgctc
     3661 ggtggagcag aaagctaccg cttatatggt gtctttaccg actttttcaa ggcgccatat
     3721 tgtacggtta gcgctcttgg aagtcaaacg aggtaatcga cctcagagac aaagttacgt
     3781 caattatttt aataatgcaa ggggttcaaa tggacgctgt caaattgttt tctgtttata
     3841 gagatagata tcgacgaaaa ttttatggag atgattgatg ataaaaaaca taaagcataa
     3901 agggcttcgg agttttgctg aaaacggtaa caccgccgga attcagcctc acttagcgaa
     3961 aaaacttcgc atgatattga ctacgctaag caccgcaaca tgtcttcaag atattgtgga
     4021 tgtaagggcg ctggggtgtc accccctaaa aggtgaacgt aaaggtgagt tttccgtaga
     4081 ggttaacgga aactggaggg tggttttcaa ggtggaagaa ccaaacgtct tagacgttga
     4141 tcttgaagat taccattagt aatcaataag taagttacta atccatccta gtggcaatat
     4201 ggatggatta gggactttat tcaaaaataa gtacctctgg aggatttatg agagccttta
     4261 tgattttgtt gttccctttg ctttgtgtag tcattgctgg ggttcttgct gttcgtggcc
     4321 tagatgggtg gggatggttc ctgtttgcag ctgttttgtg tactggtgtt gatacggtgc
     4381 agaaacatgt ctctaacaag aataattctg taagcgattg aggctttcag ggttcttcct
     4441 tgactgatta aatcgacaaa gcgtatctta tgttcgaaac gaaaaaaccg cctggggagg
     4501 cggttttttc gagggttcag taagttgggg aacttctgaa ccgtggtaac agggtgtaca
     4561 agaccgctga aaccaaattc tgtccttcca gtttagcctc agtatggctc tgacttcaag
     4621 aactctgcat cagtaatctc ttgtacaccc tcttaccaat ggccgcagcc agtggcgttt
     4681 tgttgtgtct ttccgggttg gactcaagtc gatagttacc ggataagacg ctgcggtcgg
     4741 actgaacggg gggttcgtgc atacagtcca gcttggagcg aactgccttc ccggaactga
     4801 gtgtcaggcg tggaatgaga taaacgcggc cataacagcg gagtgacacc ggtaaaccga
     4861 aaggcaggaa caggagagcg cacgagggag ccaccagggg gaaacgcctg gtatctttat
     4921 agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg
     4981 gggcggagcc tatggaaaaa cggctttgcc gtggccttct cgcttccctg ttaagcttct
     5041 tcctggcatc ctccaggaaa tttccgcccc gttcgtaagc catttccgct cgccgcagcc
     5101 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga atatatcctg tatcacatat
     5161 tctgctgacg caccggtgcc gccttttttc tcctgccaca tgaagcactt cactgacacc
     5221 ctcatccgtg ccaacatagt aagccagtat acactccgct agcgctacgt gactggttca
     5281 gggctgcgcc ccgaaacccg ccaaaagcct gacgcgcctg cggcttgtcc aaccccgcgc
     5341 cgaccgggaa aggctttccc gcccgtcccg gcgttatcag gaggccggtt tgtcagagaa
     5401 acggagcaag
//
