LOCUS       NZ_CP103603             2337 bp    DNA     circular CON 20-SEP-2023
DEFINITION  Escherichia coli strain 4848 plasmid pMB7536_7, complete sequence.
ACCESSION   NZ_CP103603
VERSION     NZ_CP103603.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28174075
            Assembly: GCF_024917355.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 58x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/20/2023 05:49:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,926
            CDSs (total)                      :: 4,805
            Genes (coding)                    :: 4,551
            CDSs (with protein)               :: 4,551
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 254
            CDSs (without protein)            :: 254
            Pseudo Genes (ambiguous residues) :: 0 of 254
            Pseudo Genes (frameshifted)       :: 86 of 254
            Pseudo Genes (incomplete)         :: 167 of 254
            Pseudo Genes (internal stop)      :: 58 of 254
            Pseudo Genes (multiple problems)  :: 52 of 254
            Pseudo Genes (short protein)      :: 3 of 254
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103603.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="4848"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMB7536_7"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2019-01-10"
                     /collected_by="MDACC"
                     /genotype="ST44"
     gene            complement(join(2271..2337,1..47))
                     /locus_tag="M5T19_RS24500"
                     /old_locus_tag="M5T19_24500"
     CDS             complement(join(2271..2337,1..47))
                     /locus_tag="M5T19_RS24500"
                     /old_locus_tag="M5T19_24500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_007772075.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPERHEKTPLAAAIGN"
                     /name="hypothetical protein"
     gene            complement(1065..1346)
                     /locus_tag="M5T19_RS24505"
                     /old_locus_tag="M5T19_24505"
     CDS             complement(1065..1346)
                     /locus_tag="M5T19_RS24505"
                     /old_locus_tag="M5T19_24505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_259384670.1"
                     /translation="MKAKFSGINGTNNIMSSQAKLRVQAGMIDNAVITRVEDGYVIIFA
                     GENSDWNGENLILSSAREPYKPRIFKSIDGAVSESERIGVKGILLDLP"
                     /name="hypothetical protein"
     gene            1485..2027
                     /locus_tag="M5T19_RS24510"
                     /old_locus_tag="M5T19_24510"
     CDS             1485..2027
                     /locus_tag="M5T19_RS24510"
                     /old_locus_tag="M5T19_24510"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_069970477.1"
                     /translation="MFGFLKKKVQEETPDTFIVGGLLFLVPRKPDDMDPIINGLVGQVE
                     KRLVSEIGIYQFFMEEIDAAGQGNDTARMLEKHSGFYPIEYKYALSQPSEMDAENSAQS
                     YLNNDVSPVLIGHFGMDIATECRCDIVAIILNKHRVLIDQIRKKVAIANHNHFVTQGDF
                     SSADKWIPVLDSLQGTS"
                     /name="hypothetical protein"
     Typing          complement(2..290)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.897"
                     /coverage="82.0"
                     /evalue="8.49e-135"
ORIGIN
        1 ttaccggata aggcgcagcg gtcggactga acggggggtt cgtgcataca gtccagcttg
       61 gagcgaactg cctacccgga actgagtgtc aggcgtggaa tgagataaac gcggccataa
      121 cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagctacc
      181 agggggaaac gcctgggatc tttatagtcc tgtcgggttt cgccaccgct gatttgagcg
      241 tcagatttcg tgatgcttgt caggggggcg gagcctatgg aaaaccggca gcccttaagt
      301 ctcttctcac acttcatacc tccacgccct ttgattgatc ccgtaacgct cgccgcagag
      361 tcatgaccga acgcagtgag cgaatgaccg aggaagcgta atttctccgg tggctgctta
      421 ggctcctgtc gctccgtact atcatctttc accacttacg ttttctgcac gtattgatgc
      481 ggacaggaaa tgtcttgtca ttaccgccgc tttgcgccag tgacaaaatc agcgggacaa
      541 gatgtgtttt tggagtaccg ccgacactcc agaatgtttc atcaggcgcc gcccgatacg
      601 ctgaatttcg tacgtacgaa atttctctgc tgttgagatc tagggcagga ttcgcaagag
      661 ggtggcggat ggcaccgtat gctgcgatgc aggatagggc aaacgccgca aaatgacgtc
      721 atttgcggcg tttgccccga aaacagaatg ttttctcctg agcctgcagc tggctctctg
      781 aacccctacc gcctcgcaaa gctcgttgct caacgcctca cgatcgcaca gtaaactgcg
      841 cgcccgttca ccgttatagg aaatgaccgt ttcctgcgaa aaagcggcct tagtgacaac
      901 atacgcgtga aactgccagc cattcacttc tgtgcatagc catgcagccg gtcagaaatc
      961 atcaggggcg tttttcaggg gtatgggcgg ggaaaggtgc cgaaaatcgg cagggggccg
     1021 cagaaacgcg ctgagaggct ctcaggcggt gcgcccaaga gatgttatgg taaatcaagt
     1081 aatattcctt taacaccaat cctttctgac tcggaaacag ctccgtctat acttttgaaa
     1141 atgcgtggtt tgtaaggttc tcgagcactg ctaagaatga ggttttcacc gttccaatct
     1201 gagttctcgc ctgcaaatat tattacatag ccatcttcta ccctagtgat gacagcattg
     1261 tcgatcatcc cagcctgaac gcgtaatttg gcttgggagg acatgatgtt atttgttccg
     1321 ttaattccag aaaattttgc tttcattatc caccttgagt attaatcaac gataatggat
     1381 atcgtatcaa ttttttaatt tgtgtcaatt tttgttttct tttgtgtaaa gtagtggctt
     1441 agttgttcaa cattgagatg tgatggctaa ctctaggggg aattatgttt ggatttttga
     1501 agaaaaaagt acaagaggaa actcctgata cttttattgt tggagggctt ctgttccttg
     1561 tgccacgtaa gccagatgac atggatccta taattaatgg acttgttggg caggttgaaa
     1621 agcgactcgt aagcgagatt ggaatttatc aattttttat ggaagaaatt gatgcagcag
     1681 gacagggtaa tgacactgcc agaatgcttg aaaaacactc tggtttttat ccgattgaat
     1741 ataaatatgc tttatcacaa ccttctgaaa tggatgctga aaatagcgct cagagctatc
     1801 tgaataatga tgtaagtcca gtcctcattg gacattttgg aatggatatt gcgacagaat
     1861 gtcgatgtga catcgttgca attattttga acaagcatcg ggtgttgatt gatcaaatca
     1921 ggaagaaagt ggcaattgcc aatcacaatc attttgtaac gcagggggat ttttcttccg
     1981 ccgacaaatg gattccagta ctggattcat tacagggaac atcataaatg tcattgacat
     2041 cgctccctca aagaaaggat cttcttgaga tcattttttt ctgcgcgtaa tctcttgccc
     2101 tgaaaacgaa aaaaccacct ggggaggtgg tttgatcgaa ggttaggtca gttggggaac
     2161 tgcttaacca ggtaactgtc ttgtgcgaga cgagtcacca aaactgtcct tccagtgtag
     2221 ccgttctcag gctcccactt caagaactcc agataaatct ctcgcacatc tcagttacca
     2281 atggctgctg ccagtggcgt tttttcgtgt ctttccgggt tggactcaag acgatag
//
