LOCUS       NZ_CP103576             1822 bp    DNA     circular CON 20-SEP-2023
DEFINITION  Escherichia coli strain 3045 plasmid pMB8093_6, complete sequence.
ACCESSION   NZ_CP103576
VERSION     NZ_CP103576.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28174015
            Assembly: GCF_024917235.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 289x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/20/2023 06:04:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,933
            CDSs (total)                      :: 4,814
            Genes (coding)                    :: 4,559
            CDSs (with protein)               :: 4,559
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 255
            CDSs (without protein)            :: 255
            Pseudo Genes (ambiguous residues) :: 0 of 255
            Pseudo Genes (frameshifted)       :: 80 of 255
            Pseudo Genes (incomplete)         :: 174 of 255
            Pseudo Genes (internal stop)      :: 52 of 255
            Pseudo Genes (multiple problems)  :: 48 of 255
            Pseudo Genes (short protein)      :: 3 of 255
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103576.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="3045"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMB8093_6"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2019-04-25"
                     /collected_by="MDACC"
                     /genotype="ST44"
     gene            1..534
                     /locus_tag="M5S79_RS24535"
                     /old_locus_tag="M5S79_24535"
     CDS             1..534
                     /locus_tag="M5S79_RS24535"
                     /old_locus_tag="M5S79_24535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005707163.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_000893324.1"
                     /translation="MLEQQFEDFEQQKRRDHQAQDQSKSAKQKRDRLNPPDWSQVTKAG
                     WNMLDSISEKVPRAFRLYSFFAQNIDKSCGAVVCDQKFLADYFGVSLRTIQYWIGDLES
                     IGAIVKIPVGIYYAYALDPYQVWRGYNKSKKYAAFNAKTLTQKDGVIDRKLRLMLSRRE
                     EFEGSLKTSNHDLD"
                     /name="replication/maintenance protein RepL"
     gene            547..834
                     /locus_tag="M5S79_RS24540"
                     /old_locus_tag="M5S79_24540"
     CDS             547..834
                     /locus_tag="M5S79_RS24540"
                     /old_locus_tag="M5S79_24540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023909281.1"
                     /translation="MRIKYESRWKLEELKLEICRSIDSLIDEYHIEYAMGINLYMNCED
                     KEFNPVDIVNEDGKNIMFVVSNNEKIQKPKKSAKKKIPAPVIELDKYRKK"
                     /name="hypothetical protein"
     Typing          complement(1077..1337)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.603"
                     /coverage="100.0"
                     /evalue="8.62e-98"
ORIGIN
        1 atgctggaac agcaatttga agatttcgaa cagcaaaaac ggcgagatca tcaggcacaa
       61 gatcaatcca aaagtgctaa gcaaaaacgc gatcggttga atccacctga ctggtcgcag
      121 gttactaagg cgggctggaa tatgttggac tctatatctg agaaggttcc cagagcattt
      181 aggctatatt ctttttttgc tcaaaatata gataaatcgt gtggggctgt tgtatgtgac
      241 caaaaattct tagctgatta tttcggtgtt agtttaagga cgatccaata ttggattgga
      301 gatttagaaa gtattggtgc catcgtgaaa ataccagtag gtatctatta tgcttatgcg
      361 ttagatccgt atcaggtatg gcgcggatat aataaatcta aaaaatatgc agcgtttaac
      421 gcaaaaacat taactcaaaa agatggcgtt attgatcgca agttgcggct catgctctct
      481 aggagagaag agtttgaagg ctcgcttaag acatctaatc atgacttgga ctaggtatga
      541 tgcgctatga gaattaaata tgaatcacgt tggaaattag aagaactaaa attagaaata
      601 tgtagatcaa tagattcgtt aattgatgaa tatcatatcg aatatgcaat gggaataaat
      661 ctatatatga attgtgaaga taaggagttt aatcccgttg atattgtcaa tgaagatgga
      721 aagaatatta tgtttgttgt tagcaacaat gaaaaaatac aaaaaccaaa aaaaagtgcg
      781 aagaaaaaaa ttcctgcacc tgtaattgag ttagataaat atcggaaaaa gtgatggcca
      841 tacagagcca gcggcaggcg gacaatccac agaatattgc atgcaatttc tagttgattg
      901 tctatcctgc attgctcttt tgccgcctca aaatcccttg cgtctttttg ctccccgttt
      961 tccagaacga ccgagccgcc acggttccgg cagcgccttg agcgcaaccg gaaaccgtct
     1021 ggggagaacc ccagaccccc atcgctgtaa catcgacggt ctacgaccac ctaccaaccg
     1081 cgtggctgcc tattccgtgc aaatgggctt tgcagggggc tgacgcggtt cctggcgacc
     1141 tgaaatcccc tggttagggg tatcggcgcg cctgacctcc taaaaccctc tgtaagcgtt
     1201 tttcgcgcct tccatgccct gatgggcatt taaaacccgt ttcgtgtgtc tgagagcttc
     1261 tgagagcgtt tgaagagcta tctggcgact catccccgaa agaacaacag cggaatcacc
     1321 gataacatgc gagaggtgtc accgctcgcc gcagccccag cgaccgaacg ttagtgagcg
     1381 agtgggcgag gaagcggaag agaaccggaa accacattga gcacgtacgc actgatgcgg
     1441 ggcgttttag cggtgaacgg actgcaaaaa catgatcgag ctgttgaacg tggagagcgc
     1501 gaaatcgggc aaaatcacgg ctgacgaggc ttgcagaaga gtaagccagt atacactccg
     1561 ctatcgctac gtgactgggt cagggcttcg ccccgacacc ccaaatgttc gatgaactaa
     1621 ttaaatcttg tgtagcaaaa aagtgctttg atagatcttt agagcaaaaa tcgacattct
     1681 cttaaaaatc aaagcattat gataccaaca tacgaaacgt tatttgcatg tgtatacgca
     1741 aacatgtttg cgtgtgttgt ttcgtaatgt acgatataaa tttcgtaagg tacgaaacaa
     1801 cgttgcaggt aaggatgcaa gt
//
