LOCUS       NZ_CP103573            12602 bp    DNA     circular CON 20-SEP-2023
DEFINITION  Escherichia coli strain 3045 plasmid pMB8093_3, complete sequence.
ACCESSION   NZ_CP103573
VERSION     NZ_CP103573.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28174015
            Assembly: GCF_024917235.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12602)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 12602)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 289x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/20/2023 06:04:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,933
            CDSs (total)                      :: 4,814
            Genes (coding)                    :: 4,559
            CDSs (with protein)               :: 4,559
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 255
            CDSs (without protein)            :: 255
            Pseudo Genes (ambiguous residues) :: 0 of 255
            Pseudo Genes (frameshifted)       :: 80 of 255
            Pseudo Genes (incomplete)         :: 174 of 255
            Pseudo Genes (internal stop)      :: 52 of 255
            Pseudo Genes (multiple problems)  :: 48 of 255
            Pseudo Genes (short protein)      :: 3 of 255
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103573.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="3045"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMB8093_3"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2019-04-25"
                     /collected_by="MDACC"
                     /genotype="ST44"
     gene            1..369
                     /locus_tag="M5S79_RS24430"
                     /old_locus_tag="M5S79_24430"
     CDS             1..369
                     /locus_tag="M5S79_RS24430"
                     /old_locus_tag="M5S79_24430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020936656.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYGL
                     NISLVAFDKALYRLRKSTDEPSLADVDSVSAAETNFSEASGPDNGFFLSGNDFMRAEEE
                     SQRVVESFRDSLSRKKGF"
                     /name="hypothetical protein"
     gene            454..1437
                     /locus_tag="M5S79_RS24435"
                     /old_locus_tag="M5S79_24435"
     CDS             454..1437
                     /locus_tag="M5S79_RS24435"
                     /old_locus_tag="M5S79_24435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.3"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_151166972.1"
                     /translation="MKKKFSIIVPVYNVEKYLRECIQSVLDQDFNDYEIICVNDGSTDG
                     SLDIILSFDDQRIKVINQENKGLSVARNVAIESSTGEYIWFLDSDDKISHGSLKLIDKI
                     TNEKNYGIIFFNTHVFFDTPELKKKGWHDYKRSTFGEMDALTFFEKEMTNKKLIVSACC
                     YVFRRELLNGNAFIPHIYHEDNHFIATLIFDNYSTICLSLDEALYLRRVRNDSIMTSKK
                     SMRHIIGYSTCIDELTKYKKTKGIQNKSFDRFVNGMVLSLFSIAETIKTKEAMNYVARY
                     KKHPDLSFKDRALINYPLIKKTYLKLKNGKVFNYLRNGIINKKSNP"
                     /name="glycosyltransferase family 2 protein"
     gene            complement(1896..2593)
                     /locus_tag="M5S79_RS24440"
                     /old_locus_tag="M5S79_24440"
     CDS             complement(join(1896..2344,2344..2593))
                     /locus_tag="M5S79_RS24440"
                     /old_locus_tag="M5S79_24440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_223368647.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEHWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            2752..4296
                     /locus_tag="M5S79_RS24445"
                     /old_locus_tag="M5S79_24445"
     CDS             2752..4296
                     /locus_tag="M5S79_RS24445"
                     /old_locus_tag="M5S79_24445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="WP_201360865.1"
                     /translation="MKNYDVFYGGIFKTITDYKLLKIQKDIKNNMTALVSSFKWVGIGQ
                     LVKIATQLVSLFYLTHLIPPSSYGLLAMSSVVMNLASIFNDMGTGAAVIQRQEASIGFY
                     NYIYRINIFTGLFVMLLVIITSPLIVVYFNTPELYPVLFLLAFSFPISSLTITHKAKLE
                     KEMNFKVIIAIEIFSSLTGMVLAIVLANLGFGVYSLIFQTLTTISISTICFLKVSGLSL
                     SLRIKNISHSDKSGVIGFSGYLLSFNLINYFSRNLDSILIGRFFSSAILGVYSIAYRIM
                     LFPLQSLTLVFSRVFLPHLSKNINNTNNTNKQDYLNALKLILSLSAPLMLGLSATSFSF
                     TRIFFDPKWHLLGDLLHWLAPTAIIQSALSTTGSVFMAYGKTRWLFFLGCIGAFLMGLS
                     FIIGVHFDIKTLVYLYFFANLINSLPAFILLKNILKFSYIELVNVFFRTISPAFLMFLL
                     IKVWQHFSFFKNASIDFSFNVALGIFSYIVFYTLFNFNEVVRTGVIHKLRCMTKLK"
                     /name="lipopolysaccharide biosynthesis protein"
     gene            complement(<4389..4653)
                     /locus_tag="M5S79_RS24450"
                     /old_locus_tag="M5S79_24450"
                     /pseudo=""
     CDS             complement(<4389..4653)
                     /locus_tag="M5S79_RS24450"
                     /old_locus_tag="M5S79_24450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /name="IS1-like element transposase"
     gene            complement(4798..5674)
                     /locus_tag="M5S79_RS24455"
                     /old_locus_tag="M5S79_24455"
                     /pseudo=""
     CDS             complement(4798..5674)
                     /locus_tag="M5S79_RS24455"
                     /old_locus_tag="M5S79_24455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946055.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(5873..6157)
                     /gene="nadS"
                     /locus_tag="M5S79_RS24460"
                     /old_locus_tag="M5S79_24460"
     CDS             complement(5873..6157)
                     /gene="nadS"
                     /locus_tag="M5S79_RS24460"
                     /old_locus_tag="M5S79_24460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_032242301.1"
                     /translation="MSFFDELKASLEEAVEIKNGTKAPARVTRYELADVRAIRENLNIT
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFDELAAH"
                     /name="nadS"
     gene            complement(6159..6491)
                     /locus_tag="M5S79_RS24465"
                     /old_locus_tag="M5S79_24465"
     CDS             complement(6159..6491)
                     /locus_tag="M5S79_RS24465"
                     /old_locus_tag="M5S79_24465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_032242302.1"
                     /translation="MSHAIEFIETTMFTRQIKQIATDDEIKELQKELIESPDKGDLIQK
                     TGGLRKIRMATGNQGKSGSARVIYFLATAEVIYLVMAYPKSTKDSITDAEKAELKKLTQ
                     RLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6636..6872)
                     /locus_tag="M5S79_RS24470"
                     /old_locus_tag="M5S79_24470"
     CDS             complement(6636..6872)
                     /locus_tag="M5S79_RS24470"
                     /old_locus_tag="M5S79_24470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_032242304.1"
                     /translation="MTEMERQLLSALESLQTSYEQQKQAWQDSYSSLQRMFETTSQALE
                     RNDRVCLNLSNQVNALHEQVERLSSDLSRLTRS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            7217..7585
                     /locus_tag="M5S79_RS24475"
                     /old_locus_tag="M5S79_24475"
     CDS             7217..7585
                     /locus_tag="M5S79_RS24475"
                     /old_locus_tag="M5S79_24475"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032242306.1"
                     /translation="MFSYLKAMYHQSKIQAELKAQIHEQTTVNAICHHPESIEIIAVCS
                     TDAYYRKRKDAAFLTTCSVLMRTLKDESVPMVLRKTAWRLLNERYQRIKLNQAYRIENF
                     LLVADFEYALEEHDELAE"
                     /name="hypothetical protein"
     gene            complement(8015..8149)
                     /locus_tag="M5S79_RS24480"
                     /old_locus_tag="M5S79_24480"
     CDS             complement(8015..8149)
                     /locus_tag="M5S79_RS24480"
                     /old_locus_tag="M5S79_24480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072003336.1"
                     /translation="MHEPPVQSDRCVLSGNYRLESNPERHAKTPLAAAIGKQDVREMQ"
                     /name="replication initiation protein"
     gene            complement(9333..9650)
                     /locus_tag="M5S79_RS24485"
                     /old_locus_tag="M5S79_24485"
     CDS             complement(9333..9650)
                     /locus_tag="M5S79_RS24485"
                     /old_locus_tag="M5S79_24485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="WP_032242326.1"
                     /translation="MKKIFILSAALIMAGCATKQYPQAVNVTGPESSAMDCKAVALEIA
                     KTRSVQQEIEETGEFDGRTVLGFLGDFGIGNGMAKGEARKKAANRLNQLEALKAAKCAD
                     Q"
                     /name="lipoprotein"
     gene            10735..10884
                     /locus_tag="M5S79_RS24490"
                     /old_locus_tag="M5S79_24490"
     CDS             10735..10884
                     /locus_tag="M5S79_RS24490"
                     /old_locus_tag="M5S79_24490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_167776965.1"
                     /translation="MPSEHDEQLDSTDTQLPGEYDLFDLMAQVTEENLHALVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            11344..12572
                     /locus_tag="M5S79_RS24495"
                     /old_locus_tag="M5S79_24495"
     CDS             join(11344..11659,11659..12572)
                     /locus_tag="M5S79_RS24495"
                     /old_locus_tag="M5S79_24495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="WP_088895425.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAASQ
                     LFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVEYG
                     RGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLR
                     IHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVPPSKR
                     AHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFNSETVQ
                     DVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGI
                     AESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRACN
                     GLSDNRCLEI"
                     /name="IS3-like element IS2 family transposase"
     Typing          10527..10700
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.571"
                     /coverage="100.0"
                     /evalue="2.6e-76"
     Typing          complement(8042..8407)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.185"
                     /coverage="89.0"
                     /evalue="2.91e-169"
     Typing          complement(10052..10560)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.51"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atggcgaatt ttaaccctgg tccaagaaca gccattgcag ctgtcaggga ttccaagaac
       61 gatatagaaa cctggaagaa aaatggtatt cgtagggaag agattgtgac tgtgctgaat
      121 gaacgttacg gcctgaatat ctcactggtt gcgtttgata aggctcttta cagattgaga
      181 aaaagtacag atgaaccgtc tcttgctgat gtggattctg tgtctgctgc agaaacgaat
      241 ttctctgaag ccagtgggcc tgacaatggt ttttttctgt ccgggaatga cttcatgcga
      301 gcagaagaag aatctcaaag agtggttgag tcattcagag atagccttag caggaagaaa
      361 ggattctgat ttattcaaca aagccgttga taaatcgcat tccataaata acaacttgat
      421 gttagtataa ctaaatattg tttggagatt actgtgaaaa aaaaattttc gataatagta
      481 cctgtatata acgttgaaaa atacttaagg gaatgtattc aatcagtatt agatcaggat
      541 tttaacgact atgagataat ctgtgtaaat gatggttcaa cagatggcag tctagatatc
      601 atactgtcat ttgatgacca aagaatcaaa gtaataaatc aggaaaataa aggcctgtcg
      661 gtggcaagaa atgtcgccat tgaatcatca actggtgaat atatatggtt tttagatagt
      721 gatgataaaa tatcacatgg aagtctaaag ttaatagaca agataaccaa tgaaaaaaat
      781 tatgggatta ttttctttaa tacacatgtt ttttttgaca ctccagaact gaaaaaaaaa
      841 ggctggcatg attataagcg ttcaacattt ggtgaaatgg atgcattaac tttctttgaa
      901 aaagaaatga cgaacaaaaa actaatagtg tcagcatgct gctatgtttt tagaagggaa
      961 ttattaaatg gcaatgcatt cattccgcat atatatcatg aggataatca ctttatagca
     1021 accctgattt ttgacaatta ttcaactata tgcctctcgt tggatgaggc gttatatctt
     1081 aggagggtaa gaaatgattc cataatgacg tctaaaaaat caatgaggca catcattgga
     1141 tattcaacat gcatcgatga gcttacgaag tataaaaaaa caaaaggcat acaaaacaag
     1201 agctttgata gatttgtgaa tggaatggtt ttgtcgttat tctctatagc ggaaacaata
     1261 aaaacaaagg aagcaatgaa ctatgtggcg agatataaaa aacaccccga cctatcattt
     1321 aaagatagag cattaattaa ttacccatta attaaaaaga cttatttaaa actaaaaaac
     1381 gggaaggtgt ttaattacct aaggaatggc attattaata aaaaaagcaa cccttaataa
     1441 aagcaaagcg ataattaata aaggtttttc tttgttgaat aaataagatt tcggataggt
     1501 atccccgtga cgggttgggt tttcaggcga tacgcacgct ttccggggct ttgttgaata
     1561 aatcgaactt ttgctgagtt gaaggatcag atcggggtca gtttggagat cggaaatttt
     1621 tgtacgttaa gcgcattatt taaccaatct tggttcgtaa ggggttgatt ttgcgtcagt
     1681 ggctatcgct tttaagttgt cgctcttgca gtatcgcatt taggttatcc gattaaggtc
     1741 aaacctctga aaatgccgta tagcgcggga gtacacccta ttgctatagg taagtctgtt
     1801 caaaaaacag gcttaccgta caataattct ctatatccaa actaacccca gatcacgcat
     1861 cttcccgaca acgcagaccg ggtaatgact ccaacttact gatagtgttt tatgttcaga
     1921 taatgcccga tgaccttgtc atgcagctcc accgattttg agaacgacag tgacttccgt
     1981 cccagccttg ccagatgttg tctcagattc agattatgtc gctcaatgcg ctgcgtatat
     2041 cgcttgctga taacgtgcag ctttcccttc aggcgtgatt catacagcgg ccagccatcc
     2101 gtcatccata ccacgacctc aaaggccgac agcaggctca gaagacgctc cagtgtggcc
     2161 agagtgcgtt caccgaagac gtgcgccaca accgtcctcc gtatcctgtc atacgcgtaa
     2221 aacagccagc gctgacgtga tttagcaccg acgtagcccc aatgttcgtc catttcagcg
     2281 cagacaatca catcacttcc cggttgtatg cgcgaggtta ccgactgcgg cctgagtttt
     2341 ttaagtgacg taaaaccgtg ttgaggccaa cgcccataat gcgtgcactg gcgcgacatc
     2401 cgacgccatt catggccata tcaatgattt tctggtgcgt accgggctga gaggcggtgt
     2461 aagtgaactg tagttgccat gttttacggc aatgagagca gagatagcgc tgatgtccgg
     2521 cagtgctttt gccgttacgc accacgcctt cagtagcgga gcaggaagga catctgatgg
     2581 aaatggaagc cacgcaagca ccttaaaatc accatcatac actaaatcag taagttggca
     2641 gcattaccca ctattggtgg agatgatcat aatgccttat aatcatacgg gatacgaact
     2701 tataaagtcc taaagtttag ctaaatcgga atctacaacg tagatgatag cttgaagaat
     2761 tatgatgtct tttatggtgg tatctttaaa accatcacgg attataaatt attgaaaatt
     2821 caaaaggata ttaaaaacaa tatgactgca ctagtcagta gttttaaatg ggttggcatt
     2881 ggacaacttg taaaaatagc aacacaattg gtttcgttat tctacttgac gcacttaatc
     2941 cccccatcga gctatggact attagcaatg tcatctgtgg ttatgaattt agcaagcata
     3001 ttcaatgaca tgggaactgg ggcggcagta attcagcgac aagaggcgtc gatcggtttt
     3061 tataattaca tatacaggat aaacattttt actgggttgt ttgttatgct gcttgtaata
     3121 ataacatccc cattaattgt cgtgtacttt aacacacccg agctataccc ggttctattt
     3181 ctattggctt ttagctttcc aatttccagc ttaacaataa ctcacaaagc aaaattagaa
     3241 aaagaaatga actttaaagt catcattgca attgaaattt tctctagcct tacaggaatg
     3301 gtgctggcaa tagttttagc taatttaggc tttggtgttt atagtttgat ttttcaaaca
     3361 ctgacaacaa tatcaatatc cacaatatgt tttttgaagg tttccgggtt gagtttatca
     3421 ttgagaatta aaaatatcag ccatagtgac aagtcaggag ttatagggtt tagtggttat
     3481 ttattgtctt ttaatttaat aaattatttt tccaggaatc ttgattctat tttaataggc
     3541 cgttttttct catccgcaat acttggggtc tattcgattg cctaccgcat tatgttattt
     3601 ccgttgcaga gcctgacgct tgttttttct agagttttct tgccacacct tagcaaaaac
     3661 ataaataaca ctaataacac taataaacaa gattacttaa atgcattaaa acttatacta
     3721 tcattaagcg cgccactgat gctaggttta tctgctacat ctttttcttt tacgaggatt
     3781 ttctttgatc ctaagtggca cctacttgga gacttattgc attggttggc acctacagcg
     3841 attatccaat cagccttgag tacaacaggt tctgttttta tggcctacgg aaaaacaagg
     3901 tggttgtttt tcttaggttg cattggcgct tttttgatgg ggttgtcttt cattataggt
     3961 gttcatttcg atataaaaac attggtctac ctttattttt ttgcaaactt aattaattca
     4021 ctcccggcat ttattttact taagaatatc ttgaagttta gttacattga attggtaaac
     4081 gtgtttttta gaactatctc cccagctttt ttgatgttct tacttattaa agtatggcag
     4141 catttttcct ttttcaaaaa tgccagcatt gatttttctt ttaatgtcgc attgggtatt
     4201 tttagttata ttgtttttta taccttgttt aatttcaatg aggttgttag gacaggagta
     4261 attcataagt taagatgcat gaccaagtta aagtgattca tttattaaat tcattaaatg
     4321 aattggtaat gactccaact tactgcttgt gttttatgtt cagataatgc ccgataccga
     4381 cttcggccta atttttttaa gtgtcgtaaa atcgtgttga ggacagtgcc cataatgcgg
     4441 gatgtggccc ggcatccaac gccattcatg gccatatcaa tgattttctg gtgtgtaccg
     4501 ggctcagagg cggtgtaagt gaactgtagt tgcttgctat gttttacgac agtgagagca
     4561 gagatagcgt tgatgtccgg tagtgctttt accgttacgc accacgcctt cagtagctga
     4621 acaggaggga taactgatgg aaatggaagc cacgggagca cctcaaaaac accattagac
     4681 actaaaccag caagttggca tcatcacaaa cagttgttac tgctggaaaa aaaacactat
     4741 caacacttaa gtaatggtga ttcaataatt aagattcagt atgtaaaata ctgaatctta
     4801 attataaatt ttcctttttc taaaaaatgt agcgcgacta atacctaaag caacccaagg
     4861 ctcccctaga gacagcctac ctcctcctga tgatttttca ccaccaactt ttcctctttt
     4921 ggcttgtata agtgaaaaat tttgcttatt aaatttcaaa aatgtccact tgcagatact
     4981 tttcgctatt cccgttactt cattctcatt caaaggcact gcaaattgca gattgtaagc
     5041 gcaggcgcgt tcatggcagg cttgtagcca ctgttcgtac tcaggccagc cctgacggat
     5101 agcgcgatat gcccactttc tggttttatc aaacagtatg cagttacgac ctaagccata
     5161 atcggcgacg atttttttat cgttcgcagc gttcaggtcg agtgattcgg ctaaccagtc
     5221 cagcgtgtag agttcaggct gccatacggg caattttcca gtgactgtgg ttcggattct
     5281 tgcaaattag ccccgaatac cccgtgtcag cgcccaattt tttacgtagc gcgctctcta
     5341 cagcagcggc gtaacgtaac ggtttcattt ttccgtctgg cgcggtgcgt atcgaggttt
     5401 tcagtgcgta cagcaggtgc gcgtgtccgt tttcggggtt ggtgatggtc agtgtcggcg
     5461 cgggggcgtt gcggtcgctc cagtcaatgg ctgcgccgat gcggtcaacg tcaaatccca
     5521 gccagaacat ggcgtgaggc tgattaaact ggatgtattt agccagaata gcgcgctctt
     5581 tccctgcgat gcggacacca aagtgtaaat catccgagaa gtacggttta tgcggcaagc
     5641 ggtcgttgaa aagttcgagt gctgcgttac gcaatgtgcc ctgaacttat ggcgggctgt
     5701 tgtctggcac ggttcatgtg ctaatatcct tcttaggttt tatacctagc cctgatttat
     5761 catgtctctt ccgccaagtt gaaatcatga ttatcggggc tttgtttttt ctgttgccgt
     5821 taaagttaca cgcgaacggc ctcgcctgtc gaatttcttc tgtcacatca gcttaatgcg
     5881 ccgccagttc atcaaaaaac tttgggttag cctgaatggt ggccagcact tttgctgcca
     5941 gtccggttgg gttacggcgt ccggtttccc agcttttgat ggtatcgagg ctggttccca
     6001 gcactttcgc catttcgctc tgagtaatat tcaggttttc tctgatagcc ctgacgtctg
     6061 ccagctcgta gcgggtcaca cgggcggggg ctttggttcc gtttttgatt tcaacggcct
     6121 cttccagaga ggctttcagc tcgtcaaaaa aactcatctt acacctcgtc tttcagtcgt
     6181 tgggtcagct tcttcagttc tgctttctct gcgtctgtga tgctgtcttt ggtgctttta
     6241 gggtaggcca tcaccagata aatcacctct gcggtggcaa ggaagtaaat cacccgcgca
     6301 ctgccgctct ttccctgatt ccccgtcgcc atcctgatct ttcgcagacc gcccgttttc
     6361 tggatgaggt cacctttgtc cggtgactcg ataagctctt tctgaagctc tttgatctca
     6421 tcatccgtgg cgatttgctt tatctgacgg gtaaacatcg ttgtctcgat aaactcaatc
     6481 gcgtgactca cggctgtctc ctgtatattt tgatgtacaa tgtacactaa gaggcgtgtt
     6541 ctgaaaagcc gcgttctgtg ccatcagacg ttacatggac tgacctcgat agcgggttgc
     6601 ttgcttctcc tgctgtaact gccgttcctg ctgctttacg agcgtgttaa tcggctcaga
     6661 tcgctgctca agcgttcgac ctgttcgtgc aaggcgttca cctgattgct caagttcaga
     6721 cataccctgt cgttgcgctc cagtgcctgt gaggtagtct cgaacatgcg ttgcaagctg
     6781 ctgtaactgt cctgccacgc ctgcttctgc tgttcgtagc ttgtttgtaa gctttctaag
     6841 gcgctcagaa gctgcctttc catctcggtc atggtttttc ctgtcctcag tgataaaaac
     6901 gtgctgtggc gtttctgtgg tgtctgacgg ggttatttcg cggtgtactt catcacggtg
     6961 tttccggctg tcgatgtgta tgtctcggtg ccatgcgctt tcttcggcag taccaccaga
     7021 taggtgttct tctgcttgtc gtccttgtaa atcgctaccc ctgcgccgct ctggggtccg
     7081 cttggaaaaa ggaaattatc ctacgctaag cgcatttttt gcacagatgg ctaccgactg
     7141 aacgtaacgt gtgagtcatg atcagaaatg tgttaactct aggaattgac acgacttact
     7201 gtttgatggg gaaatcatgt ttagctatct caaggctatg tatcatcaat caaaaattca
     7261 ggctgaactc aaggcacaga tacatgaaca aactacggtt aatgctatct gtcatcaccc
     7321 tgaatcaatc gagatcattg cggtttgttc gactgatgcg tattaccgaa aacgtaagga
     7381 cgctgctttt ctgacaacct gttctgtgct catgcgtact ttaaaagatg aatccgtacc
     7441 gatggtacta cggaaaacag catggcgatt attgaacgaa agataccaac gaattaagtt
     7501 aaatcaggct tataggatag aaaatttctt gcttgttgcc gattttgaat atgctctcga
     7561 agaacatgat gaactagcag agtagtacta gtgaggcgat gaataccgac agttaacgtg
     7621 catgtgactt aaacgtccga ttacgtacag catgtagaca acatgtccga aagtaagcaa
     7681 aaccattaac ggacagccgg atcatttcgt acatccctta cggtcagggg ttcgcgctca
     7741 atggaacgaa atcacccgtt agcttaaaac gcttaacgta ggataatttc ctttttccaa
     7801 gcggacccgc aatacagcaa ggaacttctt gagatccttt ctgttgcgtc gtaatctctt
     7861 gctctgtaaa cgaaaaaacc accctggcag gtggtttttc gaaggttagc taatcctggc
     7921 agattttcta accgcggtaa cagtcttgtg cgagacatgt caccaaatct gtcctttcag
     7981 tgtagccgca ctcaggccat cacttcaaga actcttactg catctctcgc acatcctgtt
     8041 taccaatggc cgctgccagt ggcgtttttg cgtgtctttc cggattggac tcaagacgat
     8101 agttaccgga caagacgcag cggtcggact gaacgggggg ttcgtgcata cagtccagct
     8161 tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa acgcggccat
     8221 aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagcta
     8281 ccagggggaa acgcctggga tctttatagt cctgtcgggt ttcgccacca ctgatttgag
     8341 cgtcagattc tgtgatgctt gtcagggggg cggagcctat ggaaaaccgg cagcgccgcg
     8401 gcctttcctc cctcgcttta tctcgtcttc atcgccatat gcctttgtga atattctccg
     8461 tccgctcgcc gcagccgaac gaccgagcgt agcgagtcag tgagcgagga agcggaatat
     8521 cttcggcctc acgtccggca ccttacactc cgtaactgca ttttttcata ggatgcagga
     8581 tagggctaac gccgcaaagt gacgtcacct gacgttcctg gattacaaag gttaaagcag
     8641 cggacaggaa atgttttgtg cccacagcta tgctgggtta ttcacaaaac agcgggacaa
     8701 aatgtgtttt tggagtaccg ccggcccgct aaagactcct ggttagctcc cgcatgtttg
     8761 ctatcttttt gctaccattt tgctactttt ttgctatctc cagagtcttc tggtctttcg
     8821 gttgctatcc ttttgctact tttttgctat caaaatgcta cctctccctt cttgcaataa
     8881 atgaccaagg cacgttagcg atgtatacat cgccgcgccg caagggtgcc ccttccgaat
     8941 cccgttcatc actgtgtgct gattaatcca ggttaaatcc gaccccctta gcaataaatc
     9001 ctgccatgcc tgacggcctg gccaacgcct cacggtcgca cagtaaactg cgcacccttt
     9061 caccgttatc ggtggggttc gtggtggctt tcagggcgct cgccggattt tagccgctaa
     9121 aatgacgtcc atgcgttcgt gtgcatagcc atgcagacgc gaagaaatca tcaggcgcgt
     9181 tttttcacga tatggacagg gaaagatgcc ggaaataggc aggagggcgc agaaatgccc
     9241 tgagggcgtc tgagatggtg cacgcaacgg atgttatggt aaattcagct gattggcagg
     9301 tacaaaaaaa gccccgaggg cttttttgta ccttattgat ctgcacactt tgcagccttc
     9361 agggcttcca gctgatttag ccggttagct gcttttttgc gggcttcgcc ttttgccatg
     9421 ccatttccta tcccaaaatc acccaggaaa cctaatacgg tgcgcccgtc aaattcgccg
     9481 gtttcctcaa tttcttgttg tacactgcgc gttttggcga tttccagagc taccgctttg
     9541 caatccattg cggaagattc tggaccggtg acgttcacgg cctgtggata ttgctttgtt
     9601 gcgcatcctg ccatgatcag cgctgccgat aggatgaata tttttttcaa aattatcctt
     9661 atttatttag gcatagttaa gagataccca ttttatcggc aggaaatgag ggttttttag
     9721 ttgaattttg ggaggggcaa ggttccatat tggtcgagga ttctggagct gaccggcaac
     9781 ggccagcgcg gctatgggcc atgcggtggt tgagcataga aaggatcttc ttgagttcct
     9841 ttttttctac gcgtaatctc ttgctctgaa aacgaaaaaa ccacctgggg aggtggtttt
     9901 tcgaaggtta agtcagttgg ggaactgctt aacctggtaa ctggcttcag cagagcgcag
     9961 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctata
    10021 gcaccaccta cattaactcg ctctgctaat cctgttacca gtggctgctg ccagtggcgc
    10081 tttgtcgtgt ctttccgggt tggactcaag acgatagtta ccggataagg cgcagcagtc
    10141 ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct tcccggaact
    10201 gagtgtcagg cgtggaatga gacaaacgcg gccataacag cggaatgaca ccggtaaacc
    10261 gaaaggcagg aacaggagag cgcacgaggg agccgccagg gggaaacgcc tggtatcttt
    10321 atagtcctgt cgggtttcgc caccactgat ttgagcgtca gatttcgtga tgcttgtcag
    10381 gggggcggag cctatggaaa aacggctttg ccgcggccct atcgcttccc tgttaagtat
    10441 ttccctggca tcttccagga catctccgcc ccgttcgtaa gccatttccg ctcgccgcag
    10501 tcgaacgacc gagcgtagcg agtcagtgag cgaggaagcg gaatatatcc tgtatcacat
    10561 attctgctga cgcactggtg ccgccttttt tgtcctgcca catgaagcac ttcactgact
    10621 ccctcatcag tgccaacata gtaagccagt atacactccg ctagcgctac gtgactggtt
    10681 cagggctgcg ccccgaaacc ctctaacaac ctctcagcta ctggatatca ccccatgcca
    10741 tctgaacatg atgaacaact ggattcgact gatacgcagc ttcccggcga atatgacctt
    10801 tttgatttaa tggcgcaggt tactgaagag aacctgcatg ccctggtgga ttttgggccg
    10861 cctgtcggcc gggaagcgtt atagcttttt cgcttacccg cgtacgtggt aatacgccgg
    10921 tattacatct tatttcttta ttttcatgta attacaggtg gttgtaatac gttgcgtatt
    10981 acataaataa acggggactt cagtttttat cttttaattc atgcaattgc gatgaaaatc
    11041 gcggttgcgt atggtgtatg accatttaag ggataatgta acctgacttt taaagaatga
    11101 atctatcttt ggcagcgcat gagatcgata aaaagcgttc cacatcaggc ggttcatgta
    11161 tccatgaacc ggaaaggaca aaaataagac aattgtaaga cattttttgc gttttactct
    11221 tattttggac tattatgaga caaatgtaag actagactgg ccccctgaat ctccagacaa
    11281 ccagtatcac ttaaataagt gatagtctta atactagttt ttagactagt cattggagaa
    11341 cagatgattg atgtcttagg gccggagaaa cgcagacggc gtaccacaca ggaaaagatc
    11401 gcaattgttc agcagagctt tgaaccgggg atgacggtct ccctcgttgc ccggcaacat
    11461 ggtgtagcag ccagccagtt atttctctgg cgtaagcaat accaggaagg aagtcttact
    11521 gctgtcgccg ccggagaaca ggttgttcct gcctctgaac ttgctgccgc catgaagcag
    11581 attaaagaac tccagcgcct gctcggcaag aaaacgatgg aaaatgaact cctcaaagaa
    11641 gccgttgaat atggacgggc aaaaaagtgg atagcgcacg cgcccttatt gcccggggat
    11701 ggggagtaag cttagtcagc cgttgtctcc gggtgtcgcg tgcgcagttg cacgtcattc
    11761 tcagacgaac cgatgactgg atggatggcc gccgcagtcg tcacactgat gatacggatg
    11821 tgcttctccg tatacaccat gttatcggag agctgcccac gtatggttat cgtcgggtat
    11881 gggcgctgct tcgcagacag gcagaacttg atggtatgcc tgcgatcaat gccaaacgtg
    11941 tttaccggat catgcgccag aatgcgctgt tgcttgagcg aaaacctgct gtaccgccat
    12001 cgaaacgggc acatacaggc agagtggccg tgaaagaaag caatcagcga tggtgctctg
    12061 acgggttcga gttctgctgt gataacggag agagactgcg tgtcacgttc gcgctggact
    12121 gctgtgatcg tgaggcactg cactgggcgg tcactaccgg cggcttcaac agtgaaacag
    12181 tacaggacgt catgctggga gcggtggaac gccgcttcgg caacgatctt ccgtcgtctc
    12241 cagtggagtg gctgacggat aatggttcat gctaccgggc taatgaaaca cgccagttcg
    12301 cccggatgtt gggacttgaa ccgaagaaca cggcggtgcg gagtccggag agtaacggaa
    12361 tagcagagag cttcgtgaaa acgataaagc gtgactacat cagtatcatg cccaaaccag
    12421 acgggttaac ggcagcaaag aaccttgcag aggcgttcga gcattataac gaatggcatc
    12481 cgcatagtgc gctgggttat cgctcgccac gggaatatct gcggcagcgg gcttgtaatg
    12541 ggttaagtga taacagatgt ctggaaatat aggggcaaat ccaaagacat tatgggcggt
    12601 at
//
