LOCUS       NZ_CP103566             1902 bp    DNA     circular CON 21-SEP-2023
DEFINITION  Escherichia coli O25b:H4-ST131 strain 751 plasmid pMB8236_4,
            complete sequence.
ACCESSION   NZ_CP103566
VERSION     NZ_CP103566.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28173988
            Assembly: GCF_024917975.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O25b:H4-ST131
  ORGANISM  Escherichia coli O25b:H4-ST131
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 273x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/21/2023 03:47:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,478
            CDSs (total)                      :: 5,367
            Genes (coding)                    :: 5,108
            CDSs (with protein)               :: 5,108
            Genes (RNA)                       :: 111
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 259
            CDSs (without protein)            :: 259
            Pseudo Genes (ambiguous residues) :: 0 of 259
            Pseudo Genes (frameshifted)       :: 90 of 259
            Pseudo Genes (incomplete)         :: 193 of 259
            Pseudo Genes (internal stop)      :: 43 of 259
            Pseudo Genes (multiple problems)  :: 57 of 259
            Pseudo Genes (short protein)      :: 3 of 259
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103566.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Escherichia coli O25b:H4-ST131"
                     /mol_type="genomic DNA"
                     /strain="751"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:941322"
                     /plasmid="pMB8236_4"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2019-05-06"
                     /collected_by="MDACC"
                     /genotype="ST131"
     gene            1..288
                     /locus_tag="M5R24_RS27295"
                     /old_locus_tag="M5R24_27295"
     CDS             1..288
                     /locus_tag="M5R24_RS27295"
                     /old_locus_tag="M5R24_27295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049092722.1"
                     /translation="MRIKYESRWRLEELKLEICSSIDELINKYGIEYATGINLYMNCED
                     KEFNLVDVINEDGNKIMFVVSNDEKIQKPKKIAKKKTPAPVIELDKYRKK"
                     /name="hypothetical protein"
     gene            1357..1896
                     /locus_tag="M5R24_RS27300"
                     /old_locus_tag="M5R24_27300"
     CDS             1357..1896
                     /locus_tag="M5R24_RS27300"
                     /old_locus_tag="M5R24_27300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_000893325.1"
                     /translation="MLEQQFEDFEQQKRRDHQVQDQSKNAKQKRDRLNPPDWSQVTKAG
                     WNMLDAISEKVPRAFRLYSFFAQNIDKSCGAVVCDQKFLADYFGVSLRTIQYWIGDLES
                     IGAIVKIPVGIYYAYALDPYQVWRGYNNTKKYAAFNAKTLTQKDGVIDRKLRLMLSRRE
                     EFESSLKYSNHDSEQG"
                     /name="replication/maintenance protein RepL"
     Typing          900..981
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.59"
                     /coverage="100.0"
                     /evalue="2.9e-32"
     Typing          complement(527..785)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.923"
                     /coverage="99.0"
                     /evalue="5.01e-120"
ORIGIN
        1 atgagaatta aatatgaatc acgttggaga ttagaagaac taaaattaga aatatgcagt
       61 tcaatagatg agttaattaa taaatatggt attgaatatg caacaggaat aaatctttat
      121 atgaattgtg aagataaaga atttaatctt gttgatgtta tcaatgaaga tggaaataaa
      181 ataatgtttg tcgttagcaa cgatgaaaaa atacaaaagc caaaaaaaat agcaaagaaa
      241 aaaactccag cacctgtaat tgagctagat aaatatcgaa aaaaatgacc attgagccag
      301 cggcaggcgg acaatcaggg gctacgtgtt aacgttctga ccttgattgt ctatcctgca
      361 ttgctctttt gccgcctcga atcccttgcg tgtttttgtt ccacgctctc cagaacgacc
      421 gagccgccac ggttccgata gcgccttgag cgcaaccgga aaccgtctgg ggagaacccc
      481 agacccccat cgctgtaata ccgacggtct acgaccacct accagccgaa tagctgccta
      541 tgccgtgcaa ataggctttg cagggggctg acgctgttcc tggcgacctg aaagcccctg
      601 gttaggggta tcggcgcgcc tgacctcctg agagcctctg taagcgtttt tcgcgccttc
      661 catgccctga cgggcattta aaacccgttt cgtgcgtctg agagcttctg agcgcgtttg
      721 aagggctatc tgacgactca tccctgaaag aacaacaacg ggagcaccga caacatgcga
      781 gaggtgacac cgctcgccgc aagtcgatga ccgagcgtag cgagcgaatc gacgaggaag
      841 cggaagagaa ccggaagcca cattgagcac ttacgcactg atgcggggtg tcggggtgaa
      901 gccctgacca ggtggtgaat gtctgagtgc gcgtgcgcgg tccgacattc ccacatcctg
      961 tcccgatttc tgaggctttt tagcggtgaa cggacggcaa aaccatgatg gagttgttga
     1021 acgttgagag catgaaatct ggcaaaaatc acggctggta aggcttgcag aggagtaagc
     1081 cagtatacac tccgctatcg ctacgtgact gggtcagggc tgcgccccga caccccaaaa
     1141 gcagttaggt agtgttaaat catgagcagt aaaaaaacgc tttgatagat ctttagagca
     1201 aaaattgaca tttctttaaa aatcattgac ttaagacacc ttcatacgaa acgttatttg
     1261 catgtgtata cgcaaacatg tttgcatgtg ttgtttcgta ctgtacgata taaatttcgt
     1321 aaggtgcgaa atatcattgc aggtaaggat gcaagtatgt tggaacagca atttgaagac
     1381 tttgaacagc aaaaacggcg agatcatcag gtacaagatc aatccaagaa cgctaagcaa
     1441 aaacgagatc ggttgaatcc tccagactgg tcgcaggtta ctaaggctgg ttggaatatg
     1501 ttggacgcta tatcagaaaa agttcctaga gcatttcggt tatattcttt ttttgcccag
     1561 aatatagata aatcatgtgg ggctgttgta tgtgatcaaa aattcttagc tgattatttc
     1621 ggtgttagtt taagaacgat ccaatattgg attggagatt tagaaagtat tggcgcaata
     1681 gttaaaatac cagtaggcat atattatgcc tatgcattag atccttatca ggtatggcgt
     1741 ggatataaca atactaaaaa atatgcagca tttaacgcaa aaacattaac tcaaaaagat
     1801 ggggttatag atcgtaaatt gcggcttatg ctttctaggc gtgaagagtt tgaaagttcc
     1861 ttaaagtact ctaatcatga ctcagagcag gggtgatgca ct
//
