LOCUS       NZ_CP103549             1748 bp    DNA     circular CON 21-SEP-2023
DEFINITION  Klebsiella pneumoniae strain 3987 plasmid pMB9017_1, complete
            sequence.
ACCESSION   NZ_CP103549
VERSION     NZ_CP103549.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28174045
            Assembly: GCF_024918055.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 43x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/21/2023 02:55:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,444
            CDSs (total)                      :: 5,318
            Genes (coding)                    :: 5,204
            CDSs (with protein)               :: 5,204
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 52 of 114
            Pseudo Genes (incomplete)         :: 59 of 114
            Pseudo Genes (internal stop)      :: 16 of 114
            Pseudo Genes (multiple problems)  :: 13 of 114
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103549.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="3987"
                     /isolation_source="same blood draw as MB8764"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pMB9017_1"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.706800 N 95.397100 W"
                     /collection_date="2019-07-25"
                     /collected_by="MDACC"
                     /genotype="ST307"
     gene            join(1479..1748,1..720)
                     /locus_tag="M5T27_RS27195"
                     /old_locus_tag="M5T27_27195"
     CDS             join(1479..1748,1..720)
                     /locus_tag="M5T27_RS27195"
                     /old_locus_tag="M5T27_27195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_135628868.1"
                     /translation="MKNPELQNLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDSRWMFLTLTVRNCAIGDLGDTLSRMNTAFQRLKDRKEFRPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPGMLNGKSYVKHARWVELWRECLRVSYDPNVDVRAVKPRKPKDG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKIEVKKYRRSPTKDKTESE"
                     /name="protein rep"
     Typing          complement(449..638)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.859"
                     /coverage="99.0"
                     /evalue="3.7200000000000003e-85"
ORIGIN
        1 atgtggcaag cccgtttcta ccagtccctg ccgcgtatcg tggcggatta ccccgattcc
       61 cgctggatgt tcctgaccct gaccgtgcgc aactgtgcga tcggggatct gggcgatacg
      121 ctgagccgga tgaacacggc tttccagcgg ctgaaggatc gcaaagagtt cagaccagtt
      181 cagggatgga tccgcaccac ggaagtcacc cgcggcagtg acggcagcgc acacccgcac
      241 ttccacacac tgatgatggt tccgcctggc atgcttaacg ggaagagtta cgtcaaacat
      301 gcgcgctggg ttgagctgtg gcgggagtgc cttcgggtca gctacgatcc gaatgtcgat
      361 gtccgggcag tgaaaccccg gaagccgaag gacggcgaat cgctggcctg cgcgaccgcc
      421 gaactggtgc gcggcgcggt ggccgagacc ctgaaatatt ccacgaagcc ggccgacatg
      481 gtcgccgatc ctgaatggtt cctcgaactg acccgccaga cgcacaagcg ccgctttgtg
      541 gcgaccggcg gcgcactcaa agacgtattg aaactcgatc aggaaaccga tgccgacatg
      601 gtgatcgggg atgatatttc cgagggcgat gatgacggtt cccgcatcgc gttcgagtgg
      661 aaaatcgagg tcaaaaaata ccggcgttcg ccgacgaaag ataaaaccga atcagagtaa
      721 aaacactgcg atgcaggata gggcaaacgc cgcaaaatga cgtcatccga catcattccg
      781 ccgcgttcgc ccagtgacct gcagtcggct ctggggtgtc ggggctgcgc cctgacctgg
      841 ggttttggat tgcggccatc gtgatggcgg taatacaaaa cgcacgggac aagatgtgtt
      901 tttggagtac cgccgacacg cgacggacag tccaaaatat cttggtcagg gctgcgcccc
      961 gacaccctgc caacacctca cgccgccaga ttaaaatctg ccagcgttcg ccgttcctga
     1021 aatcgtcaaa cgctaaatcg tggggtcccg ctggcttgcc agtgggttcc gatttagtga
     1081 ttgacgagcg cacccgtaaa acactgccga caggtggttg tgatcacgca gtgagcgcaa
     1141 ccacctgata agcgtttttg gggtttgtgg ggtccgattt aacgtggatt ttgggggcaa
     1201 aaaatcgcgc gcgttccttg tatttatact taagggataa ctctggcgtt aaaaataagc
     1261 ctcagccctt gtgccgcaag ggtttcagcg tgatttgcct gaaaatgact ttgaagttat
     1321 tctgtctgga gtggtcgcgt taaaagcgac ccccgcaacg acggccatcg ttcgggggcg
     1381 tgaccacctg taggaattct attttgcgac agacaacaca ggtgatacgg caatgataac
     1441 acgtcaagtg agccatcgcc cagtatgggg cggtgcgcat gaaaaatcct gaactccaga
     1501 acctcacgga ctattccccc tcagatgcgc cctgggacgc tcacagaagc gtttctgacg
     1561 acgtgggagg gatttacctg cttgcggccg aatatgagcg ctacggggcg cgtatggcct
     1621 cctgcggcgg tttactgcgt ttcgggtggt ctaccctcaa agaaaccgga gaaacgcgcc
     1681 tgaggctgcg tgaggcgcat ttctgccgtg tccggcactg tcctgtctgc cagtggaggc
     1741 gctccctg
//
