LOCUS       NZ_CP103528             1718 bp    DNA     circular CON 21-SEP-2023
DEFINITION  Escherichia coli strain 2779 - blood draw same patient as MB9267
            plasmid pMB9272_7, complete sequence.
ACCESSION   NZ_CP103528
VERSION     NZ_CP103528.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28174006
            Assembly: GCF_024918015.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 91x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/21/2023 03:46:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,256
            CDSs (total)                      :: 5,133
            Genes (coding)                    :: 4,930
            CDSs (with protein)               :: 4,930
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 203
            CDSs (without protein)            :: 203
            Pseudo Genes (ambiguous residues) :: 0 of 203
            Pseudo Genes (frameshifted)       :: 65 of 203
            Pseudo Genes (incomplete)         :: 146 of 203
            Pseudo Genes (internal stop)      :: 38 of 203
            Pseudo Genes (multiple problems)  :: 43 of 203
            Pseudo Genes (short protein)      :: 4 of 203
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103528.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2779 - blood draw same patient as MB9267"
                     /isolation_source="blood draw same patient as MB9267"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMB9272_7"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.706800 N 95.397100 W"
                     /collection_date="2019-10-28"
                     /collected_by="MDACC"
                     /genotype="ST405"
     gene            1..405
                     /locus_tag="M5S63_RS26165"
                     /old_locus_tag="M5S63_26165"
     CDS             1..405
                     /locus_tag="M5S63_RS26165"
                     /old_locus_tag="M5S63_26165"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061604172.1"
                     /translation="MKILAAMEIADCDFEHMDMDVLVGALLKLKLWDSHHHNLNMQGKR
                     SFLDDLRADGIEAIKKYEAEIESENESFAEERLILRIYQNELGLWLGRLFCGEAEIGQC
                     GVYGSADEVRTAARQSGILCDSIEIFQKDA"
                     /name="hypothetical protein"
     gene            1269..1505
                     /locus_tag="M5S63_RS26170"
                     /old_locus_tag="M5S63_26170"
     CDS             1269..1505
                     /locus_tag="M5S63_RS26170"
                     /old_locus_tag="M5S63_26170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016230891.1"
                     /translation="MTRLTNLTPAEKKFIDDAIAAAERAAGKKLNQPNRHIVLNRARAQ
                     IESQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     Typing          1028..1112
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.512"
                     /coverage="100.0"
                     /evalue="3.06e-32"
ORIGIN
        1 atgaagattt tggctgcaat ggaaatagct gattgtgatt ttgaacatat ggatatggat
       61 gtcttagtag gggctttact gaaattaaaa ttgtgggata gccaccatca caacttgaac
      121 atgcaaggta agaggagctt tctcgatgat ttaagagcgg atggtattga ggctattaag
      181 aaatacgagg ctgaaattga atctgagaat gagtcctttg cggaggagcg attaatactg
      241 aggatctatc agaacgagct tggcttatgg ttagggaggc tcttttgtgg tgaggctgag
      301 atcggtcagt gtggtgttta tggatcggct gatgaggttc gaacagctgc gcgtcaaagc
      361 ggtattttgt gcgatagcat cgaaattttt caaaaggatg cctaatgccc ccgtggtggg
      421 tacaccagaa aacggggagc ggagcgacca gcgtcaggtc gttctatgtt gttctgattg
      481 tgtgggtgtt gtgccagact gggcaatcgc atttcaccgc aactcctgcc ttgaatctca
      541 ctttatcagg tgcctgttat cgtacagcga gacggggtag agcttaactc tttcgcggct
      601 aagttacgtt tccaccgttt gcctgtttat cctgccggag tccggggcgt agacctgagt
      661 ttcaagggtg cagatatgat tttaatgtcg ttgaatggta ttaagctggt agtgacaagg
      721 ctagccagcc ctgctaggat aggattgcta cgtctgatcc tggcaggtat ctgcgaagaa
      781 ttagagtacc gaagccctgt gattctgtca cggggctttt gtacatctgt agcctgattt
      841 ttcaacaaga tccccgtcac acctcacttc atgtatgtga aaaaagttgt agtgcccgcc
      901 gcagtctcac gtccggagcg tagcgaccga gtgagcgagg aggcatcatt tcactattcg
      961 ggcatggtat tgcggacgct tctgagccac atatcacacc tgccacatga cgcactttcc
     1021 tgataaccgc acctatgcca acatattaag ccagtataca ctccgctagc gctacgtgac
     1081 tggttcaggg ctgcgccccg aaacccgcta aatccactga cgcgcctgcg gcttgtccaa
     1141 cgcctcacgg caccggatta aaatccgctc ccgttcaccg tttctgactg cctttcaccg
     1201 tcgcttacgc ttcatcgttc ctactgtgtc tggcagactg caggggctat tctcatacgg
     1261 agataaacat gacgagactg acaaacctga cccccgctga gaaaaagttt atcgacgacg
     1321 ctatagccgc tgccgaacgt gcggccggga agaagctcaa tcagcctaac cggcacatcg
     1381 tcctgaacag agcgcgtgcg cagattgagt cgcagcgtta cgccgaccgc cagcgtgcat
     1441 tacgtgagga tgagcggcag cagtccgatt ttgcctggtc acgtccgcgc gcgccgcgcc
     1501 gctgattgcg gattttggcg gtttagtggt gctgatgctg cgaaatcacc tgcggttcac
     1561 ccgcgtgcat agctatgcag tcgcgcagaa atcatcaggg gcgtttttcc gggatgtggg
     1621 tgaggaaatg tgccggaaac ggctctgatg cgtcagaatt gcgctgaggg cctgtcaggg
     1681 ggtgctgata acggatgtta tggtaaatcg gagggtgt
//
