LOCUS       NZ_CP103517            12602 bp    DNA     circular CON 21-SEP-2023
DEFINITION  Escherichia coli strain 5444 plasmid pMB9292_2, complete sequence.
ACCESSION   NZ_CP103517
VERSION     NZ_CP103517.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28174095
            Assembly: GCF_024917955.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12602)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 12602)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 209x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/21/2023 03:21:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,978
            CDSs (total)                      :: 4,858
            Genes (coding)                    :: 4,580
            CDSs (with protein)               :: 4,580
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 278
            CDSs (without protein)            :: 278
            Pseudo Genes (ambiguous residues) :: 0 of 278
            Pseudo Genes (frameshifted)       :: 87 of 278
            Pseudo Genes (incomplete)         :: 191 of 278
            Pseudo Genes (internal stop)      :: 58 of 278
            Pseudo Genes (multiple problems)  :: 53 of 278
            Pseudo Genes (short protein)      :: 3 of 278
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103517.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="5444"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMB9292_2"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2019-11-07"
                     /collected_by="MDACC"
                     /genotype="ST44"
     gene            complement(274..423)
                     /locus_tag="M5S96_RS24620"
                     /old_locus_tag="M5S96_24620"
     CDS             complement(274..423)
                     /locus_tag="M5S96_RS24620"
                     /old_locus_tag="M5S96_24620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_167776965.1"
                     /translation="MPSEHDEQLDSTDTQLPGEYDLFDLMAQVTEENLHALVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            1508..1825
                     /locus_tag="M5S96_RS24625"
                     /old_locus_tag="M5S96_24625"
     CDS             1508..1825
                     /locus_tag="M5S96_RS24625"
                     /old_locus_tag="M5S96_24625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="WP_032242326.1"
                     /translation="MKKIFILSAALIMAGCATKQYPQAVNVTGPESSAMDCKAVALEIA
                     KTRSVQQEIEETGEFDGRTVLGFLGDFGIGNGMAKGEARKKAANRLNQLEALKAAKCAD
                     Q"
                     /name="lipoprotein"
     gene            3009..3143
                     /locus_tag="M5S96_RS24630"
                     /old_locus_tag="M5S96_24630"
     CDS             3009..3143
                     /locus_tag="M5S96_RS24630"
                     /old_locus_tag="M5S96_24630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072003336.1"
                     /translation="MHEPPVQSDRCVLSGNYRLESNPERHAKTPLAAAIGKQDVREMQ"
                     /name="replication initiation protein"
     gene            complement(3573..3941)
                     /locus_tag="M5S96_RS24635"
                     /old_locus_tag="M5S96_24635"
     CDS             complement(3573..3941)
                     /locus_tag="M5S96_RS24635"
                     /old_locus_tag="M5S96_24635"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032242306.1"
                     /translation="MFSYLKAMYHQSKIQAELKAQIHEQTTVNAICHHPESIEIIAVCS
                     TDAYYRKRKDAAFLTTCSVLMRTLKDESVPMVLRKTAWRLLNERYQRIKLNQAYRIENF
                     LLVADFEYALEEHDELAE"
                     /name="hypothetical protein"
     gene            4286..4522
                     /locus_tag="M5S96_RS24640"
                     /old_locus_tag="M5S96_24640"
     CDS             4286..4522
                     /locus_tag="M5S96_RS24640"
                     /old_locus_tag="M5S96_24640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_032242304.1"
                     /translation="MTEMERQLLSALESLQTSYEQQKQAWQDSYSSLQRMFETTSQALE
                     RNDRVCLNLSNQVNALHEQVERLSSDLSRLTRS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4667..4999
                     /locus_tag="M5S96_RS24645"
                     /old_locus_tag="M5S96_24645"
     CDS             4667..4999
                     /locus_tag="M5S96_RS24645"
                     /old_locus_tag="M5S96_24645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_032242302.1"
                     /translation="MSHAIEFIETTMFTRQIKQIATDDEIKELQKELIESPDKGDLIQK
                     TGGLRKIRMATGNQGKSGSARVIYFLATAEVIYLVMAYPKSTKDSITDAEKAELKKLTQ
                     RLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            5001..5285
                     /gene="nadS"
                     /locus_tag="M5S96_RS24650"
                     /old_locus_tag="M5S96_24650"
     CDS             5001..5285
                     /gene="nadS"
                     /locus_tag="M5S96_RS24650"
                     /old_locus_tag="M5S96_24650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_032242301.1"
                     /translation="MSFFDELKASLEEAVEIKNGTKAPARVTRYELADVRAIRENLNIT
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFDELAAH"
                     /name="nadS"
     gene            5484..6360
                     /locus_tag="M5S96_RS24655"
                     /old_locus_tag="M5S96_24655"
                     /pseudo=""
     CDS             5484..6360
                     /locus_tag="M5S96_RS24655"
                     /old_locus_tag="M5S96_24655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946055.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            6505..>6769
                     /locus_tag="M5S96_RS24660"
                     /old_locus_tag="M5S96_24660"
                     /pseudo=""
     CDS             6505..>6769
                     /locus_tag="M5S96_RS24660"
                     /old_locus_tag="M5S96_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /name="IS1-like element transposase"
     gene            complement(6862..8406)
                     /locus_tag="M5S96_RS24665"
                     /old_locus_tag="M5S96_24665"
     CDS             complement(6862..8406)
                     /locus_tag="M5S96_RS24665"
                     /old_locus_tag="M5S96_24665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="WP_201360865.1"
                     /translation="MKNYDVFYGGIFKTITDYKLLKIQKDIKNNMTALVSSFKWVGIGQ
                     LVKIATQLVSLFYLTHLIPPSSYGLLAMSSVVMNLASIFNDMGTGAAVIQRQEASIGFY
                     NYIYRINIFTGLFVMLLVIITSPLIVVYFNTPELYPVLFLLAFSFPISSLTITHKAKLE
                     KEMNFKVIIAIEIFSSLTGMVLAIVLANLGFGVYSLIFQTLTTISISTICFLKVSGLSL
                     SLRIKNISHSDKSGVIGFSGYLLSFNLINYFSRNLDSILIGRFFSSAILGVYSIAYRIM
                     LFPLQSLTLVFSRVFLPHLSKNINNTNNTNKQDYLNALKLILSLSAPLMLGLSATSFSF
                     TRIFFDPKWHLLGDLLHWLAPTAIIQSALSTTGSVFMAYGKTRWLFFLGCIGAFLMGLS
                     FIIGVHFDIKTLVYLYFFANLINSLPAFILLKNILKFSYIELVNVFFRTISPAFLMFLL
                     IKVWQHFSFFKNASIDFSFNVALGIFSYIVFYTLFNFNEVVRTGVIHKLRCMTKLK"
                     /name="lipopolysaccharide biosynthesis protein"
     gene            8565..9262
                     /locus_tag="M5S96_RS24670"
                     /old_locus_tag="M5S96_24670"
     CDS             join(8565..8814,8814..9262)
                     /locus_tag="M5S96_RS24670"
                     /old_locus_tag="M5S96_24670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_223368647.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEHWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            complement(9721..10704)
                     /locus_tag="M5S96_RS24675"
                     /old_locus_tag="M5S96_24675"
     CDS             complement(9721..10704)
                     /locus_tag="M5S96_RS24675"
                     /old_locus_tag="M5S96_24675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.3"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_151166972.1"
                     /translation="MKKKFSIIVPVYNVEKYLRECIQSVLDQDFNDYEIICVNDGSTDG
                     SLDIILSFDDQRIKVINQENKGLSVARNVAIESSTGEYIWFLDSDDKISHGSLKLIDKI
                     TNEKNYGIIFFNTHVFFDTPELKKKGWHDYKRSTFGEMDALTFFEKEMTNKKLIVSACC
                     YVFRRELLNGNAFIPHIYHEDNHFIATLIFDNYSTICLSLDEALYLRRVRNDSIMTSKK
                     SMRHIIGYSTCIDELTKYKKTKGIQNKSFDRFVNGMVLSLFSIAETIKTKEAMNYVARY
                     KKHPDLSFKDRALINYPLIKKTYLKLKNGKVFNYLRNGIINKKSNP"
                     /name="glycosyltransferase family 2 protein"
     gene            complement(10789..11157)
                     /locus_tag="M5S96_RS24680"
                     /old_locus_tag="M5S96_24680"
     CDS             complement(10789..11157)
                     /locus_tag="M5S96_RS24680"
                     /old_locus_tag="M5S96_24680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020936656.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYGL
                     NISLVAFDKALYRLRKSTDEPSLADVDSVSAAETNFSEASGPDNGFFLSGNDFMRAEEE
                     SQRVVESFRDSLSRKKGF"
                     /name="hypothetical protein"
     gene            complement(11188..12416)
                     /locus_tag="M5S96_RS24685"
                     /old_locus_tag="M5S96_24685"
     CDS             complement(join(11188..12101,12101..12416))
                     /locus_tag="M5S96_RS24685"
                     /old_locus_tag="M5S96_24685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="WP_088895425.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAASQ
                     LFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVEYG
                     RGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLR
                     IHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVPPSKR
                     AHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFNSETVQ
                     DVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGI
                     AESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRACN
                     GLSDNRCLEI"
                     /name="IS3-like element IS2 family transposase"
     Typing          complement(459..632)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.143"
                     /coverage="100.0"
                     /evalue="5.58e-78"
     Typing          599..1107
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.51"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2752..3117
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.185"
                     /coverage="89.0"
                     /evalue="2.91e-169"
ORIGIN
        1 atgaaccgcc tgatgtggaa cgctttttat cgatctcatg cgctgccaaa gatagattca
       61 ttctttaaaa gtcaggttac attatccctt aaatggtcat acaccatacg caaccgcgat
      121 tttcatcgca attgcatgaa ttaaaagata aaaactgaag tccccgttta tttatgtaat
      181 acgcaacgta ttacaaccac ctgtaattac atgaaaataa agaaataaga tgtaataccg
      241 gcgtattacc acgtacgcgg gtaagcgaaa aagctataac gcttcccggc cgacaggcgg
      301 cccaaaatcc accagggcat gcaggttctc ttcagtaacc tgcgccatta aatcaaaaag
      361 gtcatattcg ccgggaagct gcgtatcagt cgaatccagt tgttcatcat gttcagatgg
      421 catggggtga tatccagtag ctgagaggtt gttagagggt ttcggggcgc agccctgaac
      481 cagtcacgta gcgctagcgg agtgtatact ggcttactat gttggcactg atgagggagt
      541 cagtgaagtg cttcatgtgg caggacaaaa aaggcggcac cggtgcgtca gcagaatatg
      601 tgatacagga tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg
      661 cggcgagcgg aaatggctta cgaacggggc ggagatgtcc tggaagatgc cagggaaata
      721 cttaacaggg aagcgatagg gccgcggcaa agccgttttt ccataggctc cgcccccctg
      781 acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa
      841 gataccaggc gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt
      901 ttaccggtgt cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt
      961 tccgggaagg cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac
     1021 tgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca cgacaaagcg
     1081 ccactggcag cagccactgg taacaggatt agcagagcga gttaatgtag gtggtgctat
     1141 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     1201 cgctctgctg aagccagtta ccaggttaag cagttcccca actgacttaa ccttcgaaaa
     1261 accacctccc caggtggttt tttcgttttc agagcaagag attacgcgta gaaaaaaagg
     1321 aactcaagaa gatcctttct atgctcaacc accgcatggc ccatagccgc gctggccgtt
     1381 gccggtcagc tccagaatcc tcgaccaata tggaaccttg cccctcccaa aattcaacta
     1441 aaaaaccctc atttcctgcc gataaaatgg gtatctctta actatgccta aataaataag
     1501 gataattttg aaaaaaatat tcatcctatc ggcagcgctg atcatggcag gatgcgcaac
     1561 aaagcaatat ccacaggccg tgaacgtcac cggtccagaa tcttccgcaa tggattgcaa
     1621 agcggtagct ctggaaatcg ccaaaacgcg cagtgtacaa caagaaattg aggaaaccgg
     1681 cgaatttgac gggcgcaccg tattaggttt cctgggtgat tttgggatag gaaatggcat
     1741 ggcaaaaggc gaagcccgca aaaaagcagc taaccggcta aatcagctgg aagccctgaa
     1801 ggctgcaaag tgtgcagatc aataaggtac aaaaaagccc tcggggcttt ttttgtacct
     1861 gccaatcagc tgaatttacc ataacatccg ttgcgtgcac catctcagac gccctcaggg
     1921 catttctgcg ccctcctgcc tatttccggc atctttccct gtccatatcg tgaaaaaacg
     1981 cgcctgatga tttcttcgcg tctgcatggc tatgcacacg aacgcatgga cgtcatttta
     2041 gcggctaaaa tccggcgagc gccctgaaag ccaccacgaa ccccaccgat aacggtgaaa
     2101 gggtgcgcag tttactgtgc gaccgtgagg cgttggccag gccgtcaggc atggcaggat
     2161 ttattgctaa gggggtcgga tttaacctgg attaatcagc acacagtgat gaacgggatt
     2221 cggaaggggc acccttgcgg cgcggcgatg tatacatcgc taacgtgcct tggtcattta
     2281 ttgcaagaag ggagaggtag cattttgata gcaaaaaagt agcaaaagga tagcaaccga
     2341 aagaccagaa gactctggag atagcaaaaa agtagcaaaa tggtagcaaa aagatagcaa
     2401 acatgcggga gctaaccagg agtctttagc gggccggcgg tactccaaaa acacattttg
     2461 tcccgctgtt ttgtgaataa cccagcatag ctgtgggcac aaaacatttc ctgtccgctg
     2521 ctttaacctt tgtaatccag gaacgtcagg tgacgtcact ttgcggcgtt agccctatcc
     2581 tgcatcctat gaaaaaatgc agttacggag tgtaaggtgc cggacgtgag gccgaagata
     2641 ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cggctgcggc gagcggacgg
     2701 agaatattca caaaggcata tggcgatgaa gacgagataa agcgagggag gaaaggccgc
     2761 ggcgctgccg gttttccata ggctccgccc ccctgacaag catcacagaa tctgacgctc
     2821 aaatcagtgg tggcgaaacc cgacaggact ataaagatcc caggcgtttc cccctggtag
     2881 ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg
     2941 gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc
     3001 tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgtcttgtcc ggtaactatc
     3061 gtcttgagtc caatccggaa agacacgcaa aaacgccact ggcagcggcc attggtaaac
     3121 aggatgtgcg agagatgcag taagagttct tgaagtgatg gcctgagtgc ggctacactg
     3181 aaaggacaga tttggtgaca tgtctcgcac aagactgtta ccgcggttag aaaatctgcc
     3241 aggattagct aaccttcgaa aaaccacctg ccagggtggt tttttcgttt acagagcaag
     3301 agattacgac gcaacagaaa ggatctcaag aagttccttg ctgtattgcg ggtccgcttg
     3361 gaaaaaggaa attatcctac gttaagcgtt ttaagctaac gggtgatttc gttccattga
     3421 gcgcgaaccc ctgaccgtaa gggatgtacg aaatgatccg gctgtccgtt aatggttttg
     3481 cttactttcg gacatgttgt ctacatgctg tacgtaatcg gacgtttaag tcacatgcac
     3541 gttaactgtc ggtattcatc gcctcactag tactactctg ctagttcatc atgttcttcg
     3601 agagcatatt caaaatcggc aacaagcaag aaattttcta tcctataagc ctgatttaac
     3661 ttaattcgtt ggtatctttc gttcaataat cgccatgctg ttttccgtag taccatcggt
     3721 acggattcat cttttaaagt acgcatgagc acagaacagg ttgtcagaaa agcagcgtcc
     3781 ttacgttttc ggtaatacgc atcagtcgaa caaaccgcaa tgatctcgat tgattcaggg
     3841 tgatgacaga tagcattaac cgtagtttgt tcatgtatct gtgccttgag ttcagcctga
     3901 atttttgatt gatgatacat agccttgaga tagctaaaca tgatttcccc atcaaacagt
     3961 aagtcgtgtc aattcctaga gttaacacat ttctgatcat gactcacacg ttacgttcag
     4021 tcggtagcca tctgtgcaaa aaatgcgctt agcgtaggat aatttccttt ttccaagcgg
     4081 accccagagc ggcgcagggg tagcgattta caaggacgac aagcagaaga acacctatct
     4141 ggtggtactg ccgaagaaag cgcatggcac cgagacatac acatcgacag ccggaaacac
     4201 cgtgatgaag tacaccgcga aataaccccg tcagacacca cagaaacgcc acagcacgtt
     4261 tttatcactg aggacaggaa aaaccatgac cgagatggaa aggcagcttc tgagcgcctt
     4321 agaaagctta caaacaagct acgaacagca gaagcaggcg tggcaggaca gttacagcag
     4381 cttgcaacgc atgttcgaga ctacctcaca ggcactggag cgcaacgaca gggtatgtct
     4441 gaacttgagc aatcaggtga acgccttgca cgaacaggtc gaacgcttga gcagcgatct
     4501 gagccgatta acacgctcgt aaagcagcag gaacggcagt tacagcagga gaagcaagca
     4561 acccgctatc gaggtcagtc catgtaacgt ctgatggcac agaacgcggc ttttcagaac
     4621 acgcctcttg gtgtacattg tacatcaaaa tatacaggag acagccgtga gtcacgcgat
     4681 tgagtttatc gagacaacga tgtttacccg tcagataaag caaatcgcca cggatgatga
     4741 gatcaaagag cttcagaaag agcttatcga gtcaccggac aaaggtgacc tcatccagaa
     4801 aacgggcggt ctgcgaaaga tcaggatggc gacggggaat cagggaaaga gcggcagtgc
     4861 gcgggtgatt tacttccttg ccaccgcaga ggtgatttat ctggtgatgg cctaccctaa
     4921 aagcaccaaa gacagcatca cagacgcaga gaaagcagaa ctgaagaagc tgacccaacg
     4981 actgaaagac gaggtgtaag atgagttttt ttgacgagct gaaagcctct ctggaagagg
     5041 ccgttgaaat caaaaacgga accaaagccc ccgcccgtgt gacccgctac gagctggcag
     5101 acgtcagggc tatcagagaa aacctgaata ttactcagag cgaaatggcg aaagtgctgg
     5161 gaaccagcct cgataccatc aaaagctggg aaaccggacg ccgtaaccca accggactgg
     5221 cagcaaaagt gctggccacc attcaggcta acccaaagtt ttttgatgaa ctggcggcgc
     5281 attaagctga tgtgacagaa gaaattcgac aggcgaggcc gttcgcgtgt aactttaacg
     5341 gcaacagaaa aaacaaagcc ccgataatca tgatttcaac ttggcggaag agacatgata
     5401 aatcagggct aggtataaaa cctaagaagg atattagcac atgaaccgtg ccagacaaca
     5461 gcccgccata agttcagggc acattgcgta acgcagcact cgaacttttc aacgaccgct
     5521 tgccgcataa accgtacttc tcggatgatt tacactttgg tgtccgcatc gcagggaaag
     5581 agcgcgctat tctggctaaa tacatccagt ttaatcagcc tcacgccatg ttctggctgg
     5641 gatttgacgt tgaccgcatc ggcgcagcca ttgactggag cgaccgcaac gcccccgcgc
     5701 cgacactgac catcaccaac cccgaaaacg gacacgcgca cctgctgtac gcactgaaaa
     5761 cctcgatacg caccgcgcca gacggaaaaa tgaaaccgtt acgttacgcc gctgctgtag
     5821 agagcgcgct acgtaaaaaa ttgggcgctg acacggggta ttcggggcta atttgcaaga
     5881 atccgaacca cagtcactgg aaaattgccc gtatggcagc ctgaactcta cacgctggac
     5941 tggttagccg aatcactcga cctgaacgct gcgaacgata aaaaaatcgt cgccgattat
     6001 ggcttaggtc gtaactgcat actgtttgat aaaaccagaa agtgggcata tcgcgctatc
     6061 cgtcagggct ggcctgagta cgaacagtgg ctacaagcct gccatgaacg cgcctgcgct
     6121 tacaatctgc aatttgcagt gcctttgaat gagaatgaag taacgggaat agcgaaaagt
     6181 atctgcaagt ggacattttt gaaatttaat aagcaaaatt tttcacttat acaagccaaa
     6241 agaggaaaag ttggtggtga aaaatcatca ggaggaggta ggctgtctct aggggagcct
     6301 tgggttgctt taggtattag tcgcgctaca ttttttagaa aaaggaaaat ttataattaa
     6361 gattcagtat tttacatact gaatcttaat tattgaatca ccattactta agtgttgata
     6421 gtgttttttt tccagcagta acaactgttt gtgatgatgc caacttgctg gtttagtgtc
     6481 taatggtgtt tttgaggtgc tcccgtggct tccatttcca tcagttatcc ctcctgttca
     6541 gctactgaag gcgtggtgcg taacggtaaa agcactaccg gacatcaacg ctatctctgc
     6601 tctcactgtc gtaaaacata gcaagcaact acagttcact tacaccgcct ctgagcccgg
     6661 tacacaccag aaaatcattg atatggccat gaatggcgtt ggatgccggg ccacatcccg
     6721 cattatgggc actgtcctca acacgatttt acgacactta aaaaaattag gccgaagtcg
     6781 gtatcgggca ttatctgaac ataaaacaca agcagtaagt tggagtcatt accaattcat
     6841 ttaatgaatt taataaatga atcactttaa cttggtcatg catcttaact tatgaattac
     6901 tcctgtccta acaacctcat tgaaattaaa caaggtataa aaaacaatat aactaaaaat
     6961 acccaatgcg acattaaaag aaaaatcaat gctggcattt ttgaaaaagg aaaaatgctg
     7021 ccatacttta ataagtaaga acatcaaaaa agctggggag atagttctaa aaaacacgtt
     7081 taccaattca atgtaactaa acttcaagat attcttaagt aaaataaatg ccgggagtga
     7141 attaattaag tttgcaaaaa aataaaggta gaccaatgtt tttatatcga aatgaacacc
     7201 tataatgaaa gacaacccca tcaaaaaagc gccaatgcaa cctaagaaaa acaaccacct
     7261 tgtttttccg taggccataa aaacagaacc tgttgtactc aaggctgatt ggataatcgc
     7321 tgtaggtgcc aaccaatgca ataagtctcc aagtaggtgc cacttaggat caaagaaaat
     7381 cctcgtaaaa gaaaaagatg tagcagataa acctagcatc agtggcgcgc ttaatgatag
     7441 tataagtttt aatgcattta agtaatcttg tttattagtg ttattagtgt tatttatgtt
     7501 tttgctaagg tgtggcaaga aaactctaga aaaaacaagc gtcaggctct gcaacggaaa
     7561 taacataatg cggtaggcaa tcgaatagac cccaagtatt gcggatgaga aaaaacggcc
     7621 tattaaaata gaatcaagat tcctggaaaa ataatttatt aaattaaaag acaataaata
     7681 accactaaac cctataactc ctgacttgtc actatggctg atatttttaa ttctcaatga
     7741 taaactcaac ccggaaacct tcaaaaaaca tattgtggat attgatattg ttgtcagtgt
     7801 ttgaaaaatc aaactataaa caccaaagcc taaattagct aaaactattg ccagcaccat
     7861 tcctgtaagg ctagagaaaa tttcaattgc aatgatgact ttaaagttca tttctttttc
     7921 taattttgct ttgtgagtta ttgttaagct ggaaattgga aagctaaaag ccaatagaaa
     7981 tagaaccggg tatagctcgg gtgtgttaaa gtacacgaca attaatgggg atgttattat
     8041 tacaagcagc ataacaaaca acccagtaaa aatgtttatc ctgtatatgt aattataaaa
     8101 accgatcgac gcctcttgtc gctgaattac tgccgcccca gttcccatgt cattgaatat
     8161 gcttgctaaa ttcataacca cagatgacat tgctaatagt ccatagctcg atggggggat
     8221 taagtgcgtc aagtagaata acgaaaccaa ttgtgttgct atttttacaa gttgtccaat
     8281 gccaacccat ttaaaactac tgactagtgc agtcatattg tttttaatat ccttttgaat
     8341 tttcaataat ttataatccg tgatggtttt aaagatacca ccataaaaga catcataatt
     8401 cttcaagcta tcatctacgt tgtagattcc gatttagcta aactttagga ctttataagt
     8461 tcgtatcccg tatgattata aggcattatg atcatctcca ccaatagtgg gtaatgctgc
     8521 caacttactg atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca
     8581 tcagatgtcc ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg
     8641 gacatcagcg ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca
     8701 ccgcctctca gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat
     8761 gtcgcgccag tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa
     8821 actcaggccg cagtcggtaa cctcgcgcat acaaccggga agtgatgtga ttgtctgcgc
     8881 tgaaatggac gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta
     8941 cgcgtatgac aggatacgga ggacggttgt ggcgcacgtc ttcggtgaac gcactctggc
     9001 cacactggag cgtcttctga gcctgctgtc ggcctttgag gtcgtggtat ggatgacgga
     9061 tggctggccg ctgtatgaat cacgcctgaa gggaaagctg cacgttatca gcaagcgata
     9121 tacgcagcgc attgagcgac ataatctgaa tctgagacaa catctggcaa ggctgggacg
     9181 gaagtcactg tcgttctcaa aatcggtgga gctgcatgac aaggtcatcg ggcattatct
     9241 gaacataaaa cactatcagt aagttggagt cattacccgg tctgcgttgt cgggaagatg
     9301 cgtgatctgg ggttagtttg gatatagaga attattgtac ggtaagcctg ttttttgaac
     9361 agacttacct atagcaatag ggtgtactcc cgcgctatac ggcattttca gaggtttgac
     9421 cttaatcgga taacctaaat gcgatactgc aagagcgaca acttaaaagc gatagccact
     9481 gaagcaaaat caacccctta cgaaccaaga ttggttaaat aatgcgctta acgtacaaaa
     9541 atttccgatc tccaaactga ccccgatctg atccttcaac tcagcaaaag ttcgatttat
     9601 tcaacaaagc cccggaaagc gtgcgtatcg cctgaaaacc caacccgtca cggggatacc
     9661 tatccgaaat cttatttatt caacaaagaa aaacctttat taattatcgc tttgctttta
     9721 ttaagggttg ctttttttat taataatgcc attccttagg taattaaaca ccttcccgtt
     9781 ttttagtttt aaataagtct ttttaattaa tgggtaatta attaatgctc tatctttaaa
     9841 tgataggtcg gggtgttttt tatatctcgc cacatagttc attgcttcct ttgtttttat
     9901 tgtttccgct atagagaata acgacaaaac cattccattc acaaatctat caaagctctt
     9961 gttttgtatg ccttttgttt ttttatactt cgtaagctca tcgatgcatg ttgaatatcc
    10021 aatgatgtgc ctcattgatt ttttagacgt cattatggaa tcatttctta ccctcctaag
    10081 atataacgcc tcatccaacg agaggcatat agttgaataa ttgtcaaaaa tcagggttgc
    10141 tataaagtga ttatcctcat gatatatatg cggaatgaat gcattgccat ttaataattc
    10201 ccttctaaaa acatagcagc atgctgacac tattagtttt ttgttcgtca tttctttttc
    10261 aaagaaagtt aatgcatcca tttcaccaaa tgttgaacgc ttataatcat gccagccttt
    10321 ttttttcagt tctggagtgt caaaaaaaac atgtgtatta aagaaaataa tcccataatt
    10381 tttttcattg gttatcttgt ctattaactt tagacttcca tgtgatattt tatcatcact
    10441 atctaaaaac catatatatt caccagttga tgattcaatg gcgacatttc ttgccaccga
    10501 caggccttta ttttcctgat ttattacttt gattctttgg tcatcaaatg acagtatgat
    10561 atctagactg ccatctgttg aaccatcatt tacacagatt atctcatagt cgttaaaatc
    10621 ctgatctaat actgattgaa tacattccct taagtatttt tcaacgttat atacaggtac
    10681 tattatcgaa aatttttttt tcacagtaat ctccaaacaa tatttagtta tactaacatc
    10741 aagttgttat ttatggaatg cgatttatca acggctttgt tgaataaatc agaatccttt
    10801 cttcctgcta aggctatctc tgaatgactc aaccactctt tgagattctt cttctgctcg
    10861 catgaagtca ttcccggaca gaaaaaaacc attgtcaggc ccactggctt cagagaaatt
    10921 cgtttctgca gcagacacag aatccacatc agcaagagac ggttcatctg tactttttct
    10981 caatctgtaa agagccttat caaacgcaac cagtgagata ttcaggccgt aacgttcatt
    11041 cagcacagtc acaatctctt ccctacgaat accatttttc ttccaggttt ctatatcgtt
    11101 cttggaatcc ctgacagctg caatggctgt tcttggacca gggttaaaat tcgccatata
    11161 ccgcccataa tgtctttgga tttgccccta tatttccaga catctgttat cacttaaccc
    11221 attacaagcc cgctgccgca gatattcccg tggcgagcga taacccagcg cactatgcgg
    11281 atgccattcg ttataatgct cgaacgcctc tgcaaggttc tttgctgccg ttaacccgtc
    11341 tggtttgggc atgatactga tgtagtcacg ctttatcgtt ttcacgaagc tctctgctat
    11401 tccgttactc tccggactcc gcaccgccgt gttcttcggt tcaagtccca acatccgggc
    11461 gaactggcgt gtttcattag cccggtagca tgaaccatta tccgtcagcc actccactgg
    11521 agacgacgga agatcgttgc cgaagcggcg ttccaccgct cccagcatga cgtcctgtac
    11581 tgtttcactg ttgaagccgc cggtagtgac cgcccagtgc agtgcctcac gatcacagca
    11641 gtccagcgcg aacgtgacac gcagtctctc tccgttatca cagcagaact cgaacccgtc
    11701 agagcaccat cgctgattgc tttctttcac ggccactctg cctgtatgtg cccgtttcga
    11761 tggcggtaca gcaggttttc gctcaagcaa cagcgcattc tggcgcatga tccggtaaac
    11821 acgtttggca ttgatcgcag gcataccatc aagttctgcc tgtctgcgaa gcagcgccca
    11881 tacccgacga taaccatacg tgggcagctc tccgataaca tggtgtatac ggagaagcac
    11941 atccgtatca tcagtgtgac gactgcggcg gccatccatc cagtcatcgg ttcgtctgag
    12001 aatgacgtgc aactgcgcac gcgacacccg gagacaacgg ctgactaagc ttactcccca
    12061 tccccgggca ataagggcgc gtgcgctatc cacttttttg cccgtccata ttcaacggct
    12121 tctttgagga gttcattttc catcgttttc ttgccgagca ggcgctggag ttctttaatc
    12181 tgcttcatgg cggcagcaag ttcagaggca ggaacaacct gttctccggc ggcgacagca
    12241 gtaagacttc cttcctggta ttgcttacgc cagagaaata actggctggc tgctacacca
    12301 tgttgccggg caacgaggga gaccgtcatc cccggttcaa agctctgctg aacaattgcg
    12361 atcttttcct gtgtggtacg ccgtctgcgt ttctccggcc ctaagacatc aatcatctgt
    12421 tctccaatga ctagtctaaa aactagtatt aagactatca cttatttaag tgatactggt
    12481 tgtctggaga ttcagggggc cagtctagtc ttacatttgt ctcataatag tccaaaataa
    12541 gagtaaaacg caaaaaatgt cttacaattg tcttattttt gtcctttccg gttcatggat
    12601 ac
//
