LOCUS       NZ_CP103507             2095 bp    DNA     circular CON 21-SEP-2023
DEFINITION  Klebsiella pneumoniae strain 5547 plasmid pMB9481_4, complete
            sequence.
ACCESSION   NZ_CP103507
VERSION     NZ_CP103507.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28174102
            Assembly: GCF_024917835.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 251x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/21/2023 03:02:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,605
            CDSs (total)                      :: 5,483
            Genes (coding)                    :: 5,334
            CDSs (with protein)               :: 5,334
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 56 of 149
            Pseudo Genes (incomplete)         :: 90 of 149
            Pseudo Genes (internal stop)      :: 24 of 149
            Pseudo Genes (multiple problems)  :: 18 of 149
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103507.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="5547"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pMB9481_4"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2019-11-24"
                     /collected_by="MDACC"
                     /genotype="ST14"
     gene            1..276
                     /locus_tag="M5R16_RS27935"
                     /old_locus_tag="M5R16_27935"
     CDS             1..276
                     /locus_tag="M5R16_RS27935"
                     /old_locus_tag="M5R16_27935"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050885152.1"
                     /translation="MASKRPRTTLLLTDELMTAIRSLSDVSGMTMSSIVVQFLEPSIPV
                     MKQLEEIVLEAKKGAAGNGRAIVGSILEGANKTLAELNREFGELDD"
                     /name="hypothetical protein"
     gene            269..1309
                     /locus_tag="M5R16_RS27940"
                     /old_locus_tag="M5R16_27940"
     CDS             269..1309
                     /locus_tag="M5R16_RS27940"
                     /old_locus_tag="M5R16_27940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01629.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid replication protein, II/X family"
                     /protein_id="WP_159174367.1"
                     /translation="MIDWITAIVPCFHETPLDSGRITKINSSGEIEWESLTAISVVGSH
                     DSSLRLKTHSINDFGNGTHIYFDGNPVKFLQGHNLFGTDNLIPLLCCVLKKITSIPELG
                     LNPTDFDVRSWEKGDFKLNRVDCTVMFDVGNTANALSWIRQAEMNATLSHRGRGQVTKG
                     STLYFGKSSRRSSVKFYAKGEEFQKHSHAAFLQLPALVDYANRGLRCEVVLRSLELNHR
                     GLSSGSAWDDLLPLQIVNDFIGRINMADITAITSDRLDGLTPSFKNVYELWKCGYDIRS
                     MHSKTAFYRYRKGLMDALGIDIATVQYKQPDNVVPFIRIIEAKPMGVPDWAIGTDLYFD
                     GAVNHG"
                     /name="phage/plasmid replication protein, II/X family"
     gene            1302..1541
                     /locus_tag="M5R16_RS27945"
                     /old_locus_tag="M5R16_27945"
     CDS             1302..1541
                     /locus_tag="M5R16_RS27945"
                     /old_locus_tag="M5R16_27945"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_074146588.1"
                     /translation="MAKKNKMTVLFCFKHNSSGLLYSRETPDNLTADDKKALYLPTVPT
                     GADFEEFLGSENARLVERMQADPAYSLRFSFRFP"
                     /name="hypothetical protein"
ORIGIN
        1 atggcatcta agcgcccgcg caccacgctt cttcttactg atgagttgat gactgcaatt
       61 cgttctcttt ctgatgtatc tggcatgacc atgtcgtcga ttgttgttca atttttagag
      121 ccatcaatcc ctgttatgaa gcagctagag gagattgtct tggaggctaa gaaaggtgct
      181 gctggtaatg gtagggctat tgttggttct attcttgagg gtgccaataa aactcttgcc
      241 gagcttaaca gggaatttgg agagttagat gattgattgg atcactgcta ttgttccttg
      301 tttccatgaa acccctttag attctgggcg aatcactaaa atcaactcat ccggtgagat
      361 tgaatgggag tctcttaccg ctatttctgt tgttggtagc catgattcat cattaagact
      421 taaaacccat tctattaatg actttggtaa cgggactcat atttattttg atgggaatcc
      481 agttaaattc cttcaaggcc ataacctttt tggaacggat aatttaattc cattgctttg
      541 ctgcgtattg aagaaaataa cctctattcc tgagcttgga cttaatccaa ctgattttga
      601 tgttcgttct tgggaaaagg gggatttcaa gttaaaccgc gttgactgta ctgttatgtt
      661 tgatgttggc aatactgcta acgcactgtc ttggattcgt caggccgaga tgaatgccac
      721 tctttctcat cgtgggcgtg gtcaggttac taaaggatct acgttatact ttggtaagtc
      781 gtcgcgccgt agctcagtga aattctatgc taagggtgag gaattccaaa agcactctca
      841 cgcagctttc ttacagcttc ctgcgctcgt tgactacgct aatcgtggtt tgcgttgtga
      901 ggttgtttta cgttcgcttg agcttaatca tcgcggtctt tcttccggtt cggcatggga
      961 tgacttgctt cctctgcaaa tagtaaatga ttttatagga cgtattaaca tggctgacat
     1021 tactgctatc acttctgata ggcttgatgg tttaacccct tcatttaaga acgtctacga
     1081 gctttggaag tgtggttatg atattaggtc tatgcactct aaaactgctt tttatcgtta
     1141 tcgtaaaggt cttatggatg ccttgggtat tgatattgcc actgtccaat acaagcagcc
     1201 tgataatgtc gttcccttca tacgcatcat agaggctaag cctatgggtg tacctgattg
     1261 ggctattggc actgatttat attttgatgg ggctgttaat catggctaaa aaaaacaaaa
     1321 tgactgttct tttttgcttt aaacataatt cctctggcct tctttattcc cgcgagacgc
     1381 ctgataattt aacggcagat gataaaaaag ccctttatct tcctaccgtt cccaccggcg
     1441 ctgattttga ggagttttta ggttctgaaa atgcccgttt ggtagagcgt atgcaagctg
     1501 acccagctta tagccttcgc tttagtttca ggttcccttg atagacgcct cgcgctctca
     1561 gattgaaatc tgacagcgtt cgccgccgcc gactcgtcaa ctgctaaatc gggggatccc
     1621 ttcagggttc cgatttagtg gttgtacgac actccctatt catcctagtt atgggtgatg
     1681 gttcacatgg tgagccatcg ccctgtaaag ccgcttttta cccccttcaa ggcgttgcct
     1741 gtggggtagg tgcgtaagcc ctgccctaca ggtgatgccg tgggggtatt ttcgttttgc
     1801 tcggtatgcc ttatttgtag atttgcaact ctgtccaggg tgatagggtt tgtcgtgcga
     1861 tcacgaatcg ggacggggat tgcccccgca gctctgcgag gaggcatacg ccgttctgat
     1921 tcgcatcctc gccattaccg atctcgatgt gcagtgtctg taatactgca catttgtccc
     1981 gattgcggaa ctactcaccc cttattgcat ttcttttttc cttcctttag tattgattca
     2041 tacggttcac accccactgc acactttgtc ccgatttcgg tacttgaggt tttat
//
