LOCUS       NZ_CP103506             9730 bp    DNA     circular CON 21-SEP-2023
DEFINITION  Klebsiella pneumoniae strain 5547 plasmid pMB9481_3, complete
            sequence.
ACCESSION   NZ_CP103506
VERSION     NZ_CP103506.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN28174102
            Assembly: GCF_024917835.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Shropshire,W.C., Konovalova,A., McDaneld,P.M., Gohel,M., Strope,B.,
            Sahasrabhojane,P., Tran,C.N., Greenberg,D.E., Kim,J., Zhan,X.,
            Aitken,S.L., Bhatti,M., Tor,S., Treangen,T., Hanson,B.M., Arias,C.A.
            and Shelburne,S.A.
  TITLE     Systematic Analysis of Mobile Genetic Elements Mediating
            Beta-lactamase Gene Amplification in Non-Carbapenemase-Producing
            Carbapenem Resistant Enterobacterales Bloodstream Infections
  JOURNAL   mSystems (2022) In press
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-AUG-2022) Infectious Diseases and Infection Control,
            University of Texas MD Anderson Cancer Center, South Campus Research
            Building 4, Houston, TX 77054, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.3-b1695
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 251x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/21/2023 03:02:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,605
            CDSs (total)                      :: 5,483
            Genes (coding)                    :: 5,334
            CDSs (with protein)               :: 5,334
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 56 of 149
            Pseudo Genes (incomplete)         :: 90 of 149
            Pseudo Genes (internal stop)      :: 24 of 149
            Pseudo Genes (multiple problems)  :: 18 of 149
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP103506.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="5547"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pMB9481_3"
                     /geo_loc_name="USA: Houston"
                     /lat_lon="29.7068 N 95.3971 W"
                     /collection_date="2019-11-24"
                     /collected_by="MDACC"
                     /genotype="ST14"
     gene            1..180
                     /locus_tag="M5R16_RS27890"
                     /old_locus_tag="M5R16_27890"
     CDS             1..180
                     /locus_tag="M5R16_RS27890"
                     /old_locus_tag="M5R16_27890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021515244.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(208..738)
                     /locus_tag="M5R16_RS27895"
                     /old_locus_tag="M5R16_27895"
     CDS             complement(208..738)
                     /locus_tag="M5R16_RS27895"
                     /old_locus_tag="M5R16_27895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938511.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(745..1476)
                     /gene="mobC"
                     /locus_tag="M5R16_RS27900"
                     /old_locus_tag="M5R16_27900"
     CDS             complement(745..1476)
                     /gene="mobC"
                     /locus_tag="M5R16_RS27900"
                     /old_locus_tag="M5R16_27900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071653262.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGASGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(1476..3437)
                     /locus_tag="M5R16_RS27905"
                     /old_locus_tag="M5R16_27905"
     CDS             complement(1476..3437)
                     /locus_tag="M5R16_RS27905"
                     /old_locus_tag="M5R16_27905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_063938523.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIVWCAGGLV
                     AGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMGTNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(5023..7212)
                     /locus_tag="M5R16_RS27910"
                     /old_locus_tag="M5R16_27910"
     CDS             complement(5023..7212)
                     /locus_tag="M5R16_RS27910"
                     /old_locus_tag="M5R16_27910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_function="GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="WP_063938521.1"
                     /translation="MNKYLGLSIVYMMYTSSVMADDSGAVSKDKNISPEETLIVHGTAV
                     SESTTEGTDSYTTQSANTASKMNLSFRDTPQSTTVITRRRMDDVAAKNIIDVVKTAPGL
                     YVSTAEGSGRPNFMSRGFNANVMYEGFSLPWNSYIPSSQTSSAIFDRVEIVRGATGLVQ
                     GAGDPSAAINMIHKRPTRQYQASTNIGAGRWNDYRGGVDVSGPLNDSATLRGRAVVDGE
                     NSETFRDSEKRRNNVIYGIMEADLTDNTLLTIGGYQQYEYNNHWWYDLPISGTGHHLNL
                     PRSTFTGNDWEFERSKSKTAFATLEQKFSNSWKLKFSTLQNWRNADLLGTATYRHKDTR
                     QSMYQQVWGSLREYNSKSYDLSLNGDYALFGRVHQFVAGTTQQKYTDKDTGYTYSPIYI
                     ENVNIFDHGSHSSKKPMKMSGDASYDVTEQKSAYAATHFLLSDNWKILLGGRLDWYEYT
                     SKGTSINTGYKVTRNITKYAGSTYNLDDHHTIYGSYTDIFNPQSSTDLNGKVIKPIVGK
                     NYETGIKGEYFNGLLNASLAVFQVDQENRARLLPDQTGCVTNGNPQCYEASGKVRTKGI
                     DFELQGMVTPLWEIGVGYTYADTRYIKDGDRSNEGKRFNTAVSPQNLFKISSMYTFDGK
                     LTNWRAGVNTYWQSKIYANITDTRDGNPVINKQKAYSTTDIIIGYTPMPQLDLQLNITN
                     VFDKVYYTSIGESIGYGTETYGEPRNVKISATYRF"
                     /name="TonB-dependent siderophore receptor"
     gene            complement(7209..8294)
                     /locus_tag="M5R16_RS27915"
                     /old_locus_tag="M5R16_27915"
     CDS             complement(7209..8294)
                     /locus_tag="M5R16_RS27915"
                     /old_locus_tag="M5R16_27915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="WP_063938519.1"
                     /translation="MAITIKSAVNVRSRVLIYSLLLFTGSAYAADIAESTFLTRDIGNG
                     LYEMSFDGKNNSLWAASSSSFDRDKGHGIVYQLEPSTLKTKSAVKIARLGFATALDEEH
                     ERLYIGNTLDGSVTVIDTLTGKELSVIPLTEGQDEAHFRPTREMVIDKKNQRLYVSSAA
                     DKGALWVIDMAVGKKIKTIDGLGHAVTGIALNKNGDNIYLVNGDGEIINLDASSYEIKK
                     RFVVEPEKKHFFLNVSLDEKNGRAFITDPDIPGVLVVDVNSGKILHRIDVINSLAILFN
                     PLRNEIYITHRNAKQVSIVDSNSYYVKTTIKTRLMPNSLSLSHDGENLYVSVKQEKKEM
                     STMKDYILKIELSKIAMEPEE"
                     /name="YncE family protein"
     gene            8671..8934
                     /locus_tag="M5R16_RS27920"
                     /old_locus_tag="M5R16_27920"
     CDS             8671..8934
                     /locus_tag="M5R16_RS27920"
                     /old_locus_tag="M5R16_27920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938517.1"
                     /translation="MSNSTHYENANFLRELAERLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            8924..9223
                     /locus_tag="M5R16_RS27925"
                     /old_locus_tag="M5R16_27925"
     CDS             8924..9223
                     /locus_tag="M5R16_RS27925"
                     /old_locus_tag="M5R16_27925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_063938515.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(9285..9662)
                     /locus_tag="M5R16_RS27930"
                     /old_locus_tag="M5R16_27930"
     CDS             complement(9285..9662)
                     /locus_tag="M5R16_RS27930"
                     /old_locus_tag="M5R16_27930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938513.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQIETHERDGLRLSDETEKMASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTSRRWWQWGKR"
                     /name="hypothetical protein"
     Typing          4238..4818
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.625"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(749..1476)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.885"
                     /coverage="100.0"
                     /evalue="3.84e-144"
ORIGIN
        1 atgactaagc aggaaaaaac tgccctcaat atggctaaat ttattaagga ccagtcgctg
       61 ctgttactcg agaaactgaa cgagctggat atggataccc cggccgacat gtgcgagcgg
      121 ctgcacgagg atgcggaaac actctggcag gtgatgcggg ataagctcgg gaaggaataa
      181 cagcaccggc atggccggtg ctgccgttta ttttttgccg aacggccacc accggcgggc
      241 gggtttgtcc tccggcagtt caggcaccgg cacgttaaaa tagcggctgt tatattcccg
      301 catcagctcc gctttcagct tctcgttcac cgcgctttcc ccgtgccgct cgctgaccac
      361 ctcaatcatc gcgctaatct gctccggtga gcacttcccg gcatcaacca cctgcacacc
      421 gttctcgatg gcgaaccgct ccaccttgcg atagaggtaa tcacacagct tcattcctgc
      481 gggtttgtcc cgttcccatt gcggattttt accggtgaaa aagtacgtgg ctttttcgtc
      541 gctgttgagc cgctcgtcat acatcccgat ggatttcatc atcgccagat gctgggcccg
      601 gtcgacaaaa cagaaaacat acgccccggt ctgccaggcg agcaccacca tatcttcaag
      661 atgctcgctg gtcggcatga tgcagcgctc gccgtcccgg tcctccgcgt cttcaataaa
      721 taccatgtac agcgccataa tccctcactc cgttgtcata aatttaaagc acgcaaacag
      781 ccgctcccgg tccagctccg ccgttttgcg ggcctcgccg gtctgtggat tcacgatttc
      841 atccaccgac aggatcgccc gctgcaccct gacctgcgtc gcccggtccg ggcagatgta
      901 ccagatttca tcgatgccgt tcgctttgcg attgaacaga tacccggaca ccacctcctg
      961 atagcgcctg cggctcttca cggtgcgctc cacctcaaac gccaccgttc tgccgtccag
     1021 ctccgccagc gcgtccggca ccttcatccc cttcagggcc atacggtgca ggtaccgcca
     1081 gccggaggcc ccccgggcct ccatggcgag acgcagggac tgcacggcca gccggtgcgg
     1141 cagctgcgcc gccgacaccc ttgacggctg aaacggaata acgtccgtca gcggttcgtc
     1201 ctcgtcgaac gagaacgcca ccccgtgcgg cgtcagtccc cacacgctga cctgacggcc
     1261 gtcaaccggg tatttcgccc ggaccagcag ccctttcgcc accaggctgt ccaggaagcg
     1321 gtaaaccggc tgcaccgccg caatccccag ccgtcgcgca accagctccg ccgtggaaca
     1381 cagctcttcc cgcagccagc gcagggtcag ccgggttttc tccgcgttcc gcgcctgccg
     1441 cttcgcgtgg ctgacgttgt atcgctccag tgccatcagt acagccccgc aaaatcatca
     1501 tcacccggcc gctgccgccc ggcctccggc tggtactccg ccagcgggtc cgttttcgcc
     1561 agcaccggga agcgctgcag cgggatttcc cggcgttcaa cccgcagcgg gctgctgaac
     1621 gccatccggg ccacgcccag cccggtcatc accgcaaacc cgtcaggcag gtgctgcagg
     1681 gtgttcacgt caaacagcgg ccgcgcctcc ttctgaatga agctgtcccc gctgatatgc
     1741 tccacgttcc ccgcctcccg gctggcccgg cggcgctcca cgtccacccg gatttccccg
     1801 gtctggcccg ccgcccactg cgcggactcc tggctggccg cccggtagaa ccagcggatc
     1861 ggcgtgttat ccagcaccgt ggttttcaca aactcagcgg gcagatcctg gccgcactgc
     1921 ccgaagtcgc ccagcgactg gtgcgcgagc agcaggttgc agtcccgact gcgcagcgtc
     1981 cccagcgcgt tcagcacgta cttcgacagc aggtatttaa tctcatcgag catgatgctc
     2041 gcgtgcggcc acctccggaa ctcatcccgc gcaatgatga tttgcgcgca gcgggcaaac
     2101 agcatcttct gcacccggat caccgcctca tcgtccatcg agccgatgac gtacaggcag
     2161 ccgccgccgt tgattatccc cgccacgtcg atgccgctgt ccgtctgcag gacgctcagg
     2221 tcggccacgt tctccagctt ggtgatcagc cccttcacgt cctttttcag ggcctccggc
     2281 agcgcgtatg ccgccgccag cacctgctga atgtttgccc cctgcgggaa ctgctccgca
     2341 atcagcttcg ccgctttctg ctcgctgatg cggtagtggt ccgccacgtc gccggtctca
     2401 ctcaggttaa acccggcaac cagcagattc ttcagggcgt actggtcgag atcgcgaaac
     2461 agattcagct gcgccggttt ccccggccgc agatccagca gggtgaagtc cgggcagtgc
     2521 gcccggaaga cgtgcggcgc ccacgcgtcc cctttggggt cgaagtacac caccgcgtcc
     2581 ccgaaatcgc gtacgcagcg aatgagcgcg ttggtgccca tcacgctctt gccgctcccg
     2641 ggcaggccga ggatctgaat attggtcttg cgccaggtat cacgcgggac cagcaccggc
     2701 tggccctgct catccttacc cagaaacacc gcctgctgtt tccgggcctg ctctgtggaa
     2761 aaatcggcca gcgcgttaac cggtaccggc tccccgaaat gcaggtggcg gacgtcggtc
     2821 atttctccgg tccgctggct gctgcgccgg ctctcctggt tcagtttcgc attcaccgcc
     2881 tcgtcagcgg ccgcaaaatg accgtcaagc agcagaaatg ccagcagccg cgccagaagg
     2941 atgccggcga caagccctcc ggcacaccag acaatcccga acaggttagc cggctgcagc
     3001 aggtaataat ctgcgttcgc gtagaggtcc ggacgcagga cggtttctcc ccagctctcg
     3061 ataaacacgg cgacaaccag cccccccaga agggccagac cggcagcgcc ggtcagccag
     3121 tacgcgtagt tccagacatg ccgccgtgcc ctgaccatcc gcccgggcga atccagcgaa
     3181 gccagcaccg aaaatggcgc agtccggtta ttatcgcgct tatagcgatc ggcatactcc
     3241 cgtcgctcct tatgactcag ccccacaaaa cgcatccgtc cggcccagag ttcagccaga
     3301 ccgaaaaaca ggccgataac caggaaagcc ccggccagac cgtaatactc cctgtacagc
     3361 cagctgaccc acgggtaaat ctgccgggcc agctccggga tatgccactg ccaggcaagc
     3421 gaatccgtat taaacatgca atctctctcc ttcagacgac gggtttaccg gcgtggtgtg
     3481 cagcccctgc tgcacaagcc gcggacatgc cccgaggcat ggcggcggcg gtcacgtccg
     3541 tggtgattgc attcagcttt caccacggag gccagcctct ttccgcccca agcggaaaag
     3601 gcgcagcccc ggaggaacaa cgagcacgcc ccagcgtgtg agaggaaaag gcggcacccc
     3661 agtgcggtct tttccggtgt tccggccccc gtcagagttc gccatagcga gcggctgacc
     3721 acccgcaaaa tcaggagcag ctttgctgct tcgtcaggtg cgttcagcac cgtgggtttt
     3781 tgtgggtggt caggcgtgag cctgacgggg gccgggtgaa tttctggcgt ggccggcgtc
     3841 cagccgagct gagccagaaa gagggggaaa gaccgtgcgt cggtggcgtc cagccagcgt
     3901 aagcagggga tttcctcctg aagtcagcca cgccccagcg gggatgacct gcatttctcc
     3961 gcaaggagaa aaaagcaaca gagataggtt aacgtaaaat attcagcaga aaaccgacag
     4021 acttcaccta tctgaatgat ttatcggcag ggaagggaaa ggcggtaaca cggtgcaata
     4081 tgtcacatcc gcgcgtccgg tggccgtctg cgggaggggg gatattgcgc ctcctccctc
     4141 actcagtcgc tacgctccgg ccgtgagact gcggcgggcg ctgctaactc tcgcaaagtt
     4201 acccacagat tccgtggata agcaggggac taacatgtga ggcaaaacgg cagggccgcg
     4261 ccggtggcgt ttttccatag gcttcgccct cctgccagaa ttcacataaa cagacgcttt
     4321 ttcggtgcat ctgtgggagc cgtgaggctc aaccatgaat ctgacagtac gggcgaaacc
     4381 cgacaggact taaagatccc caccgtttcc ggcgggtcgc tccctcttgc gctctcctgt
     4441 tccgaccctg ccgtttaccg gatacctgtt ccgcctttct cccttacggg aagtgtggcg
     4501 ctttctcata gctcacacac tggtatctcg gctcggtgta ggtcgttcgc tccaagctgg
     4561 gctgtaagca agaactcccc gttcagcccg actgctgcgc cttatccggt aactgttcac
     4621 ttgagtccaa cccggaaaga cacgcaaaaa cgccactggc agcagccatt ggtaactggg
     4681 agttcgcaga ggatttgttt agctaaacac gcggttgctc ttgaagtgtg cgccaaagtc
     4741 cggctacact ggaaggacag atttggttgc tgtgctctgc gaaagccagt taccacggtt
     4801 aagcagttcc ccaactgact taaccttcga tcaaaccacc tcaccaggtg gttttttcgt
     4861 ttacagggca aaagattacg acgaaccatg gaggatctca agaagatcct ctacatgatt
     4921 tgattaacca tcaactgaag tggcaaacag ggggttgtac gccggaacca caaaagacgg
     4981 atttataggg gcaaatccga ggagaagacc caatccgcat ggttagaatc tataggttgc
     5041 actaatttta acatttcttg gttctccata tgtttccgtg ccatatccaa tcgattcacc
     5101 tatggatgta taataaacct tatcaaagac attagttata ttgagctgta aatccagttg
     5161 tggcattgga gtatagccaa taataatgtc agttgttgaa taagcctttt gtttattaat
     5221 aaccggattg ccatcccttg tgtctgttat gttagcatat attttacttt gccagtacgt
     5281 gtttactcct gctcgccagt ttgttaattt tccgtcaaag gtgtacatgc tcgaaatctt
     5341 aaataaattt tgtggcgaca cggcagtatt gaatcgcttg ccttcattac tacgatctcc
     5401 atcttttata tatcgcgtat ctgcatatgt atatcccacc ccaatttccc agagtggcgt
     5461 aaccatgccc tgaagttcga aatctatccc ttttgtgcgt actttaccgc tagcctcata
     5521 gcattgagga ttgccattag taacacaccc tgtctgatcg ggaagcagcc tggctcgatt
     5581 ttcctggtct acctgaaata ccgccagaga ggcattaagc aagccgttaa aatactcacc
     5641 cttaatacct gtctcataat tcttaccaac aataggcttg atcactttac cattgaggtc
     5701 ggtgcttgac tgtggattga atatatcggt atagctccca taaatggtat gatgatcgtc
     5761 aagattgtat gttgaacctg catatttggt gatatttctg gtaactttat aacctgtatt
     5821 aatggatgta cctttacttg tatattcata ccagtcaaga cggccaccca gcaatatttt
     5881 ccagttatca ctaagtagaa aatgagttgc tgcgtaggcg cttttttgtt cagtgacgtc
     5941 gtaggatgca tcacctgaca tcttcatggg ttttttcgaa gaatgtgaac catgatcaaa
     6001 tatattaaca ttctcaatat atattgggga atatgtataa cctgtatctt tatcggtgta
     6061 tttttgctga gttgttccag ccacgaattg atgaacacga ccaaataaag catagtcccc
     6121 attaagcgac aggtcatagc ttttggaatt atattccctc agtgatcccc atacttgctg
     6181 atacatactt tgtcgggtat ctttatgccg gtatgttgcc gtccctaaca ggtcagcatt
     6241 tctccagttt tgcagcgttg aaaatttcag cttccagcta ttactgaact tctgctccag
     6301 agttgcaaat gcagttttac ttttacttct ttcaaactcc cagtcattgc ctgtaaacgt
     6361 cgagcgagga agattaaggt ggtgtccggt accagaaata ggtaagtcat accaccaatg
     6421 attgttatat tcatactgct gatatccccc aatcgtaagt agggtattat cagtcagatc
     6481 tgcctccata attccgtaaa tgacgttatt cctgcgtttc tcactatccc tgaaggtctc
     6541 actgttttcg ccatccacaa ctgcccttcc gcgtaaggtc gcgctgtcat tcagtggtcc
     6601 tgatacatca accccacccc ggtagtcatt ccaccgtccg gcaccgatgt tagtgctggc
     6661 ctgatattgc cgggtcggac gcttgtgtat catatttatc gcggctgaag ggtctccggc
     6721 accctgaaca agacctgttg ccccgcggac tatctccacc cggtcaaaaa tagctgaact
     6781 agtttgtgaa cttggaatgt atgaattcca cggcaggcta aatccttcat acatgacatt
     6841 agcattaaat cctctggaca taaaattcgg tcttccggaa ccttctgctg tactgacata
     6901 taatccagga gcggttttaa ccacatcaat aatatttttt gctgctacat catccattct
     6961 tcgtcgcgta atgactgtag ttgactgagg tgtatcccgg aatgacagat tcatctttga
     7021 tgctgtattg gctgactggg ttgtgtatga atctgttcct tctgttgtgc tttcagaaac
     7081 agcggtccca tgaacaatta atgtttcttc tggtgaaata tttttatctt tactaactgc
     7141 gccagaatca tcagccataa cagatgatgt atacatcata tatactatgc tcagccctaa
     7201 atatttattc attcttctgg ctccatagca attttagata attcgatttt gagtatgtaa
     7261 tccttcatag tactcatctc ttttttttct tgttttacac taacgtacag attttcaccg
     7321 tcatgtgata aagacagact gtttggcatc aggcgggttt ttatcgttgt ttttacgtaa
     7381 taagaattac tgtccacaat gctgacctgt tttgcattac ggtgcgtgat atagatttcg
     7441 ttccgtaaag gattaaataa aatcgccagt gaatttataa catcaattct gtgtagtatt
     7501 tttcccgagt ttacatcaac aaccagcacc ccgggaatat ccgggtcggt aataaatgca
     7561 cgtccgtttt tttcatccag acttacatta aggaaaaagt gttttttctc aggctctaca
     7621 acaaaccgtt tttttatttc atagctactg gcatccaggt taataatctc accatccccg
     7681 ttaaccagat aaatattatc cccattttta tttaacgcaa ttccagtaac agcgtgcccc
     7741 agtccatcaa tggttttaat tttcttcccg acagccatgt caataaccca cagagcccct
     7801 ttatctgcgg cgctggagac ataaagacgc tgattttttt tgtcaatgac catttctctg
     7861 gtcggtctga aatgagcctc atcctgtccc tcagttaacg gaattactga aagttctttt
     7921 ccggttagag tatcaatgac cgtcactgag ccgtccagag tattgccgat ataaagtcgc
     7981 tcatgttcct catccagtgc tgtggcaaac ccaagacgtg cgattttaac ggcagatttt
     8041 gttttcagag tggatggttc taactgataa acaattccgt ggcccttatc tctgtcgaat
     8101 gaagacgatg atgctgccca gagactatta tttttgccat caaaagacat ttcatacaga
     8161 ccattgccga tatcacgagt caggaatgtt gattcagcta tatcagccgc atacgctgaa
     8221 ccagtaaaca gtagtaaaga gtatattaat actctgctac ggacattaac ggcggattta
     8281 atcgtgattg ccatacttac atttccttac aataaaagaa acaagaatat agcaaaagtg
     8341 ataatgatta tcactaccat taatcacatg atttaccata acgtcgcatt acgcgcaccg
     8401 ctcagacggt ctgagggcat ttccggcggc tacgggataa aaccggcaac aaaccacccg
     8461 gaaaaccgcc ggaaacgcgc ctgataaatt ttaacccgct gcatggctat gcacgcaact
     8521 gcatgtcgtg cagtttgacg taaaatgggt gtcgtaagcc cttttttacc agctttcgtc
     8581 ttcatcagcc tgactgacac gagcatattc tttcagaccg ccccctgcta aactccttca
     8641 tatcgacgta tcagcccggg aggctccaca atgagcaaca gtacacacta tgaaaacgcc
     8701 aatttcctgc gggaactggc cgagagactg ccccgcatac tgcctgaagg tggcccggac
     8761 aaagctgcac tgttgcagcg cctggctaac gaagaactgg cccaggcaga atatgaagat
     8821 caggttcgcg ctaaagtgac cgccgcccgc gctgacacgc gcccgggcat gaccacggaa
     8881 caactccgcc agagactgca tggccgctat caggaactac gcgatgccgt atgaggtgga
     8941 atggaagcag ggagccatcg aggatgtgac agcattgttt gactacattg cggaaaattc
     9001 atccctgtgg gatgcgcaga acgttaccga tcgagttctc gctgcagcgg acaaactggc
     9061 cgacttcccg cgcctatatg aagtggactc acgctatggc gaggacgttc gccggatcag
     9121 tctgatggga cagcatgtcc tctatgatgt tgatgaccag accaggaaag tccgggttct
     9181 ggccgtggtt ggccagcgcc agaatcccca taccgttcgc tgataaccgg tgatgggggc
     9241 gcaattcatt gcgtggccgt cgccggttgg acaagcacag cgcgtcagcg ttttccccac
     9301 tgccaccatc ggcgcgatgt tccggccggt gtctctgctg tctctggttt gtgttccagc
     9361 agtctcatgg cctgcatttc ctgccgcagt ccggccacct cctccctgag tgcttttatc
     9421 tccgcgatca acgcgtcgga ggccattttt tcagtctcat ccgacagtct caacccgtcc
     9481 cgctcgtgag tctcaatctg tcttaattca ccgtacaccc ggataagttc gctggtatcc
     9541 accacccgac caccatcagc ttcagtacgg tatgatacgc ggcctttcgc catatcccta
     9601 tacagggaac tccgggcttt ccccgtcagt tccgtcgcct gtctcaccgt cacccacgcc
     9661 attgtctcac ctccggtttt gagacagtat acccgacagg aagcacacca cacccggaag
     9721 gacagaaaac
//
